사회심리학적인 요인들, 흡연습관, 운동습관이 음주운전 의도에 미치는 영향

이기형,권용만,Lee, Ki Hyeong,Kwon, Yong Man 한국데이터정보과학회 2015 한국데이터정보과학회지 Vol.26 No.1

본 연구의 목적은 음주운전으로 인한 자동차 사고를 줄이기 위한 방법으로 음주운전의 의도에 영향을 주는 여러 가지 요인들을 조사하였다. 본 논문에서는 흡연습관과 운동습관과 같은 운전자의 생활방식뿐만 아니라 사회심리학적인 요인들을 조사에 포함시켰다. 조서결과 음주운전자의 사회심리학적인 특성 중에서 행동통제력지각이 음주운전의 의도에 가장 큰 영향을 미치고, 뒤이어 음주운전의 의도에서 흡연습관, 운동습관 등의 순으로 영향을 미치는 것으로 나타났다. 이러한 연구 결과는 흡연습관과 운동습관과 같은 운전자의 생활방식이 음주운동에 대한 태도나 주관적인 규정보다 음주운전의 의도에 더 큰 영향을 미친다는 것으로 해석될 수 있다. 따라서 음주운전자 교육과정에 금연습관과 운동습관을 포함시킴으로서 음주운전을 감소시킬 수 있도록 프로그램을 개선하여야 한다. The purpose of this paper was to investigate various factors influencing the intentions of drink-driving from multiple perspectives, in order to uncover ways to reduce the number of motor accidents caused by drink-driving. We examined sociopsychological aspects as well as driver's life styles such as smoking habit and exercise habit. Perception of behaviour controls among drink-driver' sociopsychological characteristics had the highest influence on the intentions of drink-driving, followed by influence of smoking and exercise on the intentions of drink-driving. This finding indicates that driver' life style such as smoking habit or exercise habit influences more on the intentions of drink-driving than attitude toward drink-driving or subjective regulations, which affirms that driver' life style such as smoking habit or exercise habit has significant effects on the intentions of drink-driving. Therefore, it is concluded that rehabilitative curriculum for drink-drivers should include a program to diminish drink-driving through nonsmoking and exercise habit.

진행성 핵상 마비에서의 국소 뇌혈류

이명철(Myung Chul Lee),이경한(Kyung Han Lee),윤병우(Byung Woo Yoon),이원용(Won Yong Lee),이기형(Ki Hyeong Lee),이상복(Sang Bok Lee),전범석(Beom S . Jeon) 대한핵의학회 1996 핵의학 분자영상 Vol.30 No.1

N/A Progressive supranuclear palsy (PSP) is a parkinson-plus syndrome characterized clinically by supranuclear ophthalmoplegia, pseudobulbar palsy, axial rigidity, bradykinesia, postural instability and dementia. Presence of dementia and lack of cortical histopathology suggest the derangement of cortical function by pathological changes in subcortical structures in PSP, which is supported by the pattern of behavioral changes and measurement of brain metabolism using positron emission tomography. This study was done to examine whether there are specific changes of regional cerebral perfusion in PSP and whether there is a correlation between severity of motor abnorma- ility and degree of changes in cerebral perfusion. We measured regional cerebral perfusion indices in 5 cortical and 2 subcortical areas in 6 patients with a clinical diagnosis of PSP and 6 healthy age and sex matched controls using Tc-99m-HMPAO SPECT. Compared with age and sex matched controls, only superior frontal regional perfusion index was significantly decreased in PSP (p〈0.05). There was no correlation between the severity of the motor abnormility and any of the regional cerebral perfusion indices (p〉0.05). We affirm the previous reports that perfusion in superior frontal cortex is decreased in PSP. Based on our results that there was no correlation between severity of motor abnormaility and cerebral perfusion in the superior frontal cortex, nonmotoric symptoms including dementia needs to be looked at whether there is a correlation with the perfusion abnormility in superior frontal cortex.

후코이단에 의한 니코틴의 코티닌 전환 효과

이경호,이기형,Lee, Keyong Ho,Rhee, Ki-Hyeong 한국식품영양학회 2014 韓國食品營養學會誌 Vol.27 No.4

본 연구에서는 nicotine 분해에 효과에 대하여 해양식물 유래의 후코이단을 이용하여 시험관에서 직접 혼합법 및 세포주 시험에서 nicotine의 cotinine으로의 전환능을 측정하였다. 직접 혼합법 시험결과, 후코이단 $1{\mu}g/mL$에서 시간이 경과함에 따라서 nicotine의 cotinine으로의 전환 정도가 대조군 대비 증가하여 시험 종료 시점에서 15배의 증가율을 나타내었다. 세포주을 이용한 nicotine의 분해능 시험 결과, 시험 종료 시점에서 대조군 대비 6배의 cotinine 증가율을 나타내었다. 양성 대조군으로 사용한 녹차 추출물 대비 nicotine 분해능은 우수한 것으로 평가되었다. 그러나 후코이단의 항산화능은 녹차 추출물과 대비하여 낮았으며, 항산화 주요 기능을 갖는 폴리페놀 및 플라보노이드 성분 역시 낮았다. 본 시험 결과가 나타내는 바는 후코이단의 nicotine의 cotinine으로의 전환능에 대한 입증을 하였으며 이는 금연 보조 역할을 할 수 있는 기능성 소재로 사료된다. This study aims to find the correlation the low-molecule fucoidan for cancer prevention with an accelerated formation of cotinine. In the presence of fucoidan, a nicotine to cotinine coversion was studied in established assay, direct mixture method and Hep-G2 cell line method. Fucoidan of $1{\mu}g/mL$ showed the potential effect for converting nicotine to cotinine in the direct mixture method compared to control. Increase of conversion rate at the treatment of fucoidan is exhibited as 15 times compared to control. In Hep-G2 cell treatment, fucoidan showed the potential activity for converting nicotine to cotinine as 6 times compared to control. Therefore, fucoidan was shown to be effective in the conversion of nicotine into cotinine even though it is not higher content of polyphenol and flavonoid than its of green tea extract.

MAPKs의 활성화 기전을 나타내는 항염증 소재의 탐색

이경호,이기형,Lee, Keyong Ho,Rhee, Ki-Hyeong 한국식품영양학회 2015 韓國食品營養學會誌 Vol.28 No.3

Lonicera japonica 에탄올 추출물의 처리에 의하여 LPS에 의해서 활성화된 RAW 264.7 세포에서 NO의 생성량과 TNF-${\alpha}$, IL-$1{\beta}$ 및 IL-6와 같은 염증성 사이토카인의 분비를 억제하였고, MAPK family인 ERK, p38 및 JNK의 인산화와 $I{\kappa}-B{\alpha}$의 분해를 억제하였다. LPS로 유도한 endotoxin shock 동물실험에서 Lonicera japonica 에탄올 추출물 20 mg/kg에서 LPS로 유도한 endotoxin shock에 대한 생존율을 3배 이상 증가시켰으며, 생존시간도 1.3~1.4배 증가시켰다. The aim of this study was to evaluate the potential anti-inflammorty properties of herbs via MAPKs such as ERK, p38, JNK. Fifty-one kinds of each herbal medicine, that were extracted with ethanol, were used in the inhibitory assay of cytokine (TNF-${\alpha}$, IL-$1{\beta}$ and IL-6) and NO. of these, 10 species of herbal medicines, Angelica dahurica, Atractylodes lancea, Cnidium officinale, Duchesnea chrysantha, Oldenlandia diffusa, Lonicera japonica, Paeonia lactiflora, Pinus thunbergii, Rehmannia glutinosa and Rubus coreanus, were screened as potential inhibitors (< $300{\mu}g/mL$) of NO, TNF-${\alpha}$, IL-$1{\beta}$ and IL-6. Among the 10 species, Lonicera japonica showed potential anti-inflammatory effects Lonicera japonica extract of $200{\mu}g/mL$ inhibited the phosphorylation of ERK, p38 and JNK. In addition, Lonicera japonica extract at 20 mg/kg increased survival rate from LPS-induced endotoxin shock by 3 fold.

포공영 추출물이 납 및 수은에 노출된 랫드 혈청의 중금속 및 혈액지표에 미치는 영향

이경호 ( Kyeong Ho Lee ),이기형 ( Ki Hyeong Rhee ) 한국환경농학회 2012 한국환경농학회지 Vol.31 No.3

본 연구에서는 포공영의 에탄올 추출물의 중금속 중독에 의한 손상 억제 효과를 측정하였다. 현대 사회에서 환경으로부터 오염이 우려되는 납과 수은을 실험동물에 노출시켰고, 납과 수은에 노출된 흰쥐에서 포공영이 이러한 중금속 노출로 영향을 받는 체중변화, 간 및 신장에 미치는 영향을 혈액세포분석 및 혈액 내의 생화학적 분자에 대한 조사하였다. 실험 결과, 포공영을 투여 받은 실험군은 중금속 투여로 인하여 발생하는 체중감소의 개선효과가 나타났고, 혈액세포의 변화에 있어서도 중금속 노출로 감소되었던 RBC의 수를 증가시켰고, 증가된 WBC 및 platelet의 수를 정상수준으로 회복시켰다. 간 및 신장의 손상 지표들로 측정한 Bilirubin, ALP, GOT, GPT, LDH, ceatinine 및 BUN에 있어서도 중금속 노출로 증가된 수치를 농도의존적으로 감소시켰다. 또한 혈액내에서의 중금속 잔존량을 측정한 결과, 포공영 추출물 투여로 인하여 농도의존적으로 그 잔존량이 감소하였다. 따라서, 포공영 에탄올 추출물은 체내 노출된 납과 수은에 대한 독성을 완화시켜주는 것으로 사료된다. BACKGROUND: The purpose of this study was that serum metabolic and hematological variables were measured to investigate the detoxication effect of Taraxacum mongolicum extract on male albino rat exposed with lead and mercury. METHODS AND RESULTS: For this study, Pb and Hg-exposed(50 ppm) albino rats was used, and the treatments were carried out in three doses of 100 mg/kg, 200 mg/kg and 500 mg/kg as dry weight of T. mongolicum extract. T. mongolicum extract could improved the body weight gain and feed efficiency ratio, except to food intake. The levels of biochemical factors elevated by Pb-Hg mix exposure, which are Bilirubin, Alkaline phosphatase(ALP), glutamic oxaloacetate transaminase(GOT), glutamic pyruvate transaminase (GPT), lactate dehydrogenase(LDH), ceatinine and blood urea nitrogen(BUN), were significantly reduced in all treated groups as compared to Pb-Hg mix exposure alone. T. mongolicum extract was shown to suppress the accumulation of Hg and Pb in serum by dose dependent manner, CONCLUSION: Therefore, this study suggest that T. mongolicum extract might have the potential effect to minimize the toxic effects of Pb and Hg.

감초 추출물의 Dextran Sulfate Sodium 유도 마우스 궤양성 대장염 억제효과

이경호 ( Keyong Ho Lee ),이기형 ( Ki Hyeong Rhee ) 한국식품영양학회 2010 韓國食品營養學會誌 Vol.23 No.4

The aim of this study was to evaluate the anti-inlfammatory effects of glycyrrhiza glabra Linne extract on ulcerative colitis induced by 3% dextran sulfate sodium in mice. The experimental animals were divided into six groups: control(normal), DSS-induced colitis(control), 1㎎/㎏, 10㎎/㎏, and 100㎎/㎏ of glycyrrhiza glabra Linne extract, and 150㎎/㎏5-aminosalicylic acid(5-ASA)(positive control). We evaluated the pathological disease activity index(DAI), change in weight, colon mucosa damage and myeloperoxidase(MPO) in colon mucosa. Treatment with 10㎎/㎏ and 100㎎/㎏ of glycyrrhiza glabra Linne extract led to significant loss of body weight, the decrease of MPO activity and clinical symptoms such as DAI and histological change. In particular, 100㎎/㎏ Glycyrrhiza glabra Linne extract led to markedlygreater improvement than 150㎎/㎏ 5-ASA treatment. These results suggest that glycyrrhiza glabra mediated anti-inflammatory action on colorectal sites may be a useful therapeutic approach to ulcerative colitis.

GSTM1과 GSTT1, 그리고 CYP1A1, CYP2E1 다형성이 폐암발생에 미치는 영향에 대한 환자-대조군연구

남홍매,강종원,배장환,최강현,이기형,김승택,원중희,김용민,김헌,Nan, Hong-Mei,Kang, Jong-Won,Bae, Jang-Whan,Choe, Kang-Hyeon,Lee, Ki-Hyeong,Kim, Seung-Taik,Won, Choong-Hee,Kim, Yong-Min,Kim, Heon 대한예방의학회 1999 예방의학회지 Vol.32 No.2

1997년 3월부터 1998년 6월까지 충북대학교병원 내과에 입원하여 치료를 받은 폐암환자 98명과 암 아닌 다른 질환을 가진 대조군 98명을 대상으로 흡연, 음주, 여러 가지 질병과거력 등을 포함한 생활습관과, GSTM1과 GSTT1, 그리고 CYP1A1, CYP1E1 유전자 다형성 양상을 조사하여 다음과 같은 결론을 얻었다. 1. GSTM1의 결손은 환자군이 67.01%, 대조군이 58.16%로 확인되었으며, OR(95% CI)이 1.46(0.82-2.62)으로 폐암 발생에 대해 유의한 영향을 미치지 않는 것으로 나타났다. 2. GSTT1의 결손은 환자군이 58.76%, 대조군이 50.00%로 확인되었으며, OR (95% CD가 1.43(0.81-2.51)으로 폐암 발생과 관련이 없는 것으로 나타났다. 3. CYP1A1 유전자 다형성은 Ile/Ile, Ile/Val, Val/Val 환자군이 각각 59.18%, 35.71%, 5.10%, 대조군이 각각 52.04%, 45.92%, 2.04%로 CYP1A1 유전자 다형성과 폐암 위험도 사이의 관련성은 유의하지 않은 것으로 나타났다$(x^2trend=0.253,\;p-value>0.05)$. 4. CYP1E1 유전자 다형성은 c1/c1, c1/c2, c2/c2 형 이 환자군에서 각각 50.00%, 42.86%, 7.14%, 대조군에서 각각 66.33%, 30.61%, 3.06%로 CYP1E1 활성이 폐암 발생에 유의한 영향을 미치는 것으로 나타났다$(x^2trend=5.783,\;p-value<0.05)$. 특히 환자군이 대조군에 비하여 아주 드문 대립유전자인 c2형이 더 많은 것으로 나타났다. 5. 폐암과 밀접한 연관이 있는 흡연습관의 OR(95% CI)이 3.03(1.58-5.81)으로 확인되어, 폐암의 위험인자로 재확인 되었다. 6. GSTM1, GSTT1, CYP1A1, CYP2-E1과 흡연습관을 포함한 다변량 분석에서 흡연습관만이 유의한 폐암의 위험인자로 나타났다. 이 결과로부터 위의 4가지 유전자의 다형성이 폐암발생에 미치는 영향은, 흡연을 포함한 환경적 요인에 비하여 크지 않을 것으로 판단된다. Objectives: This study was performed to investigate sweets of genetic polymorphisms of glutathione S-transferase M1 (GSTM1), glutathione S-transferase M1 (GSTT1), cytochrome P450 1A1 (CYP1A1) and cytoehrome P450 2E1 (CYP2E1) on lung cancer development. Methods: Ninety-eight lung cancer patients and 98 age-sex matched non-cancer patients hospitalized in Chungbuk National University Hospital form March 1997 to August 1998, were the subjects of this case-control study. Direct interview was done and genotypes of GSTM1, GSTT1, CYP1A1 and CYP2E1 were investigated using multiplex PCR or PCR-RFLP methods with DNA extracted from venous blood. Effects of the polymorphisms of GSTM1, GSTT1, CYP1A1 and CYP2E1, lifestyle factors including smoking, and their interactions on lung rancor were statistically analyzed. Results: GSTM1 was deleted in 67.01% of the cases and 58.16% of the controls, and the odds ratio(95% CI) was 1.46(0.82-2.62). GSTT1 deletion was 58.76% for the lung cancer patients and 50.00% for the controls[OR:1.43(0.81-2.51)]. The frequencies of lle/lle, lle/Val and Val/Val of the CYP1A1 polymorphisms were 59.18-18%, 35.71%, and 5.10% for the cases, and 52.04%, 45.92%, 2.04% for the controls, respectively. Risk of lung cancer was not associated with polymorphism of CYP1A1 ($x^2trend=0.253$, p-value>0.05). The respective frequency of c1/c1 c1/c2, c2/c2 genotypes for CYP2E1 were 50.00%, 42.86%, 7.14% for the lung cancer patients, and 66.33%, 30.61%, 3.06% for the controls $(x^2trend=5.783,\;p<0.05)$. c2 allele was a significant risk factor for lung cancer. We also observed a significant association of cigarette smoking history with lung cancer risk. The odds ratio(95% Cl) of cigarette smoking was 3.03(1.58-5.81). In multiple logistic analysis including genotypes of GSTM1, GSTT1, CYP1A1 and CYP2E1, and smoking habit, only snaking habit came out to be a significant risk factor for lung cancer. Conclusion: Genetic polymorphisms of GSTM1, GSTT1, CYP1A1 and CYP2E1 are not so strongly associated with lung cancer as lifestyle factors including cigarette smoking.

Hollow Fiber Assay을 이용한 고량강 추출물의 항종양 효과

이경호 ( Keyong Ho Lee ),이기형 ( Ki Hyeong Rhee ) 한국식품영양학회 2011 韓國食品營養學會誌 Vol.24 No.4

The purpose of this investigation was to evaluate anti-tumor activity and detect what compounds affect its activity form Alpinia officinarum Hance. Two fractions, methanol and ethylacetate, were isolated by Amberlite XAD-2 resin column chromatography from methanol extract of the rhizomes of Alpinia officinarum. In hollow fiber assay, the methanol extract and methanol fraction were found to inhibit the tumor growth against colon tumor cell lines such as Colo-320, HCT116 and WiDr. Three diarylheptanoids [5-hydroxy-1,7-diphenyl-3-heptanone, 5-hydroxy-7-(4``-hydroxy-3``-methoxyphenyl)-1-phenyl- 3-heptanone and 3,5-dihydroxy-1,7-diphenylheptane] and two flavonoids [galangin and kaempheride] were isolated and identified from the methanol fraction, which is higher activity than ethylacetate fraction. Among these diarylheptanoids and flavonoids, 3,5-dihydroxy-1,7-diphenylheptane, galangin and kaempheride as active components on anti-tumor activity were mainly posited in methanol fraction.

증례 : 혈액종양 ; 백혈병의 증거 없이 발견된 과립구 육종 2예

이규호 ( Kyu Ho Lee ),김현 ( Hyun Kim ),최관용 ( Guan Yong Choi ),이주희 ( Ju Hee Lee ),한혜숙 ( Hye Suk Han ),이기형 ( Ki Hyeong Lee ),김승택 ( Seung Taik Kim ) 대한내과학회 2009 대한내과학회지 Vol.77 No.2

과립구 육종은 백혈병의 진행 도중 또는 이후에 합병증으로 생기는 경우가 대부분이나 드물게 백혈병의 증거 없이 비전형적으로 발견되는 경우가 있을 수 있다. 저자들은 혈액이나 골수에서 백혈병의 증거 없이 소장 및 부신을 포함한 다발성 복강 내 종괴로 내원한 과립구 육종 1예와 흉추에 발생하여 양측 하지 마비를 유발한 과립구 육종 1예를 경험하였기에 보고하는 바이다. Granulocytic sarcoma (GS) is a rare extramedullary tumor composed of immature myeloid precursor cells. GS is associated with acute or chronic myeloid leukemia; it has rarely been reported in non-leukemic patients. We report two cases of GS with unusual presentations; neither had bone marrow involvement. The first case was a GS presenting with multiple intra-abdominal masses in a 43-year-old man. Computed tomography (CT) of the abdomen showed masses in the small bowel and right adrenal gland. The second case was a patient with a GS presenting with symptoms of spinal cord compression in a 32-year-old man. Magnetic resonance imaging (MRI) showed an extradural mass in the spinal canal at the T3-T5 levels. Blood smear and bone marrow studies were normal. These cases show that GS should be included in the differential diagnosis of intra-abdominal masses and extradural spinal cord tumors, even in the absence of leukemia. (Korean J Med 77:236-240, 2009)

향나무 추출물의 Galectin-3 저해에 의한 암 전이 억제 효과

이경호 ( Keyong Ho Lee ),이기형 ( Ki Hyeong Rhee ) 한국식품영양학회 2012 韓國食品營養學會誌 Vol.25 No.4