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Purification and Serology of Potato Virus S
이순형,이기운,정봉조,Lee Soon Hyung,Lee Key Woon,Chung Bong Jo Korean Society of Applied Entomology 1977 한국식물보호학회지 Vol.16 No.3
감자바이러스 S(PVS)의 진단, 동정 및 씨감자의 검정에 이용할 항혈청을 만들기 위하여 이병주로부터 PVS를 순수분리 순화하여 항혈청을 제조하였다. PVS는 지표식물파 전자 현미경으로 순수 분리하여 Nicotiana debneyii에서 증식하여 순화하였다. 순화된 PVS의 순화도는 1.18mg/ml이었으며 이것을 1.5ml씩 7일 간격으로 5회 .토끼에 주사하였으며 마지막 주사후 10일에 채혈하여 항혈청을 분리하였다. 제조된 PVS항혈청의 역가는 미량침강법에 의하여 1/2048로 나타났다. he study was conducted to produce an antiserum of potato virus S for identification and screening of seed-potatoes. Potato virus S was isolated from infected plants and identified by means of indicator plants and electro microscopy. Isolated potato virus S was multiplied in Nicotiana deebneyii and the virus was purified by a modified method that was developed through this study. The purity of potato virus S was 1.18mg/ml. Purified potato virus S was injected into rabbit intravenously once a week for 5 weeks. Antiserum was collected 10 days after the last injection. The produced antiserum was determined to have a titer of, 1/2048 by means of microprecipitin tests.
이영훈 ( Yeong Hoon Lee ),임승택 ( Seung Taek Lim ),윤영남 ( Young Nam Yoon ),전명기 ( Myeong Gi Jeon ),윤홍태 ( Hong Tae Yun ),고종민 ( Jong Min Ko ),이수헌 ( Su Heon Lee ),이기운 ( Key Woon Lee ),백인열 ( In Youl Baek ) 한국콩연구회 2013 韓國콩硏究會誌 Vol.29 No.1
국내 콩에서는 SMV, SbDV, AMV, CMV, CPMV, SYMMV, SYCMV와 PSV가 보고되었다. 과거 이들 바이러스 중에서 SMV가 심각한 피해를 입히고 있으며, 90%이상 우점하는 것으로 보고되었다. 하지만, 최근 SMV의 발생률은 50%정도로 낮아졌으며, SYMMV, SYCMV와 PSV 등에 의한 여러 가지 바이러스 병해가 피해를 주고 있는 것으로 확인되었다. 최근 기후 및 재배환경의 변화와 국제 농산물 교역으로 인해 병 발생 양상이 급속하게 변하고 있음을 나타내는 단적인 예인 것이다. 또한, 새로운 매개층과 전염원의 발생으로 신종 또는 미보고 바이러스 발생이 늘어나고 있지만, 콩과 같은 주요 작물의 병해 발생 상황 및 피해 양상 구명에 대한 연구는 미흡한 실정이다. 이러한 이유로 국내 콩에서 발생하는 바이러스 병해의 확인을 위해 8도19지역에서 193점의 시료를 채집하였다. 채집된 시료들은 SMV, SYMMV, SYCMV, SbDV, PSV, BCMV, AMV, PEMV, CMV, CCMV, TSV, BBWV2, BYMV와 CPMV 14종에 대한 정밀 진단을 위하여 각각의 종특이 프라이머를 이용하여 RT-PCR 진단이 수행되었다. 그 결과 채집된 시료의 86%가 바이러스에 감염된 것으로 확인 되었으며, SMV 141, SYMMV 14, PSV 8과 SYCMV 5점이 확인되었다. 대구와 나주에서 채집된 2점의 시료들에서는 SYMMV와 SYCMV가 복합감염 되어있었다. 나머지 미동정 시료에 대한 정밀 분석과 지속적인 발생상황 조사가 이루어 져야 할 것이다. It had been reported that soybean viral diseases are Soybean mosaic virus (SMV), Soybean dwarf virus (SbDV), Alfalfa mosaic virus (AMV), Cucumber mosaic virus (CMV), Cowpea mosaic virus (CPMV), Soybeun yellow rnottle mosaic virus (SYMMV), Soybean yellow common mosaic virus (SYCMV) and Peanut stunt virus (PSV) in Korea. Among these viral diseases, SMV caused severe damage to soybean in Korea, Although SMV occurred more than 90% in the past, recently several viruses such as SYMMV, SYCMV, SbDV and PSV have been reported in Korea, It means that the incidence of viral diseases are changing in soybean. To identify the viruses infecting soybean in Korea, the 193 samples with viral symptoms were collected in 19 areas of 8 provinces. And then the RT-PCR assay was conducted to detect 14 different viruses such as SMV, SYMMV, SYCMV, SbDV, PSV, BCMV, AMV, PEMV, CMV, CCMV, TSV, BBWV2, BYMV and CPMV. The results indicated that about 86% of samples were identified as Virus-infected, Among 193 soybean samples, 141 SMV, 8 PSV, 14 SYMMV, 5 SYCMV were detected. Two samples were coinfected with SYMMV and SYCMV. The rest of them were likely to express the mosaic similar to virus-induced symptoms. Therefore, the identifications of the unknown samples have been performed by the dererminatlon of the nucleotide sequences of the genomic RNAs.
李起運 慶北大學校 1984 論文集 Vol.38 No.-
This experiment was conducted to obtain basic informations for an integrated control of the disease by studying the occurrence, damage disease cycle comprising virusinsect vector-plant, host range, and, testing methods for varietal resistance. The results obtained are as follow. It is expected that the disease be limited to southern area below the line connecting Daejon, Boeun and Uljin in Korea because viruliferous vectors are not able to overwinter in the area above the line. As a result, rice dwarf does not occur in seedling bed in the northern area although the disease may be found in the paddy field by infection by viruliferous vectors moved from southern area. High yield strains have been planted extensively for their high yield. In southern area ratoons of the high yield strains remain alive 8-30 days after harvest. The ratoons of infected plants serve insect vectors for acquisition of the virus by feeding and contribute to the increased number of the overwintered viruliferous insect vectors. Foot path serves as a habitat for insect vectors and contribute to higher disease occurrence near the foot path than in the middle of the field. When rice plants were inoculated at different leaf stages with the virus, high yield strains showed less infection than japonica strains in vegetative growth stage, but difference between the two strains was minor inreproductive growth stage. Incubation period in the plant was also longer in high yield strains than in Japonica strains. Inoculation of japonica strain rice at 3-9 leaf stage resulted in a high percentage of withering, whereas high yield strains inoculated at 5 leaf stage or later showed relatively low percentage of withering. Heading was possible only when rice plants of both high yield and japonica strains were inoculated at 7 to 9 leaf stages or later and ripening only at 9 leaf stage or later. Thirty five species of plants including cereals and weeds were tested for host of the rice dwarf virus. Twenty nine of them were identified to be hosts of the virus. There were Oryza sativa L. Nagdongbyeo, Hordeum sativum Jess. Olbori, Triticum aestivum L. Dahong mil, Avena sativa L. Zea mays L. Suwoen╋29, Setaria italica Beauv, Alopecurus aequalis Sosbol, Echinochloa hispidula Nakai, Echinochloa echinata Nakai, Glyceria acutiflora, Panicum miliaceum, Phleum pratense L., Poa annua L., Echinochloa crusgalli var. frumentosa, Secal cereal L. Digitaria sanguinalis L. var. cilialis, Digitaria sanguinalis Scopoli var. aultinervis Honda, Eleusine indica Gaert., Zoysis japonica Steudel, Arthraxon hispidus (Thunberg), Leersia sayanuka, Setaria viridis Beauvis var. purpurascens, Eragrostis curvular Mees, Eleocharis acicularis L., Eriocaulon atrvm Nakai, Eriocaulon robustius Nees., Elymus arenarius L. Coix agrestis Lour, Isachne globosa Kuniz. When the rice dwarf virus was double inoculated with other viruses such as rice stripe virus and rice black-streaked dwarf virus, only 6.7 to 17.0% of double infection was obtained. But the rate was relatively low compared to single inoculation indicating that infection is inhibited by double inoculation. Only 0.5 to 1.6% of barley seedlings infected with rice dwarf virus servived winter. This suggests that overwintering of the virus on infected plants is almost impossible. In fluctuation in occurrence of vectors, peak period was late July. Percentage of infected hills rapidly increase early in July and late in August. This suggests that overwintered adults, nymphs of second generation, and nymphs of third generation served as transmission of the virus. The percentage of viruliferous vectors was the highest in the second generation ones. Insect vectors can acquire infectivity by feeding infected plants only for 48 hours. Incubation period in the insect body was about 10 to 18 days. Vectors could be infective by injection with juice of infected plants or viruliferous insects. The transmission activity was high in nymphal stage and virus affinity was relatively highl in ecotype Ⅱ-a vectors collected from southern part of the peninsula. Inoculating seedlings at 3 leaf stage with an inoculum level of 1 to 2 insects was effective. On the physiopathological ecology of viruliferous vectors, viral infection reduced oviposition period of female vectors, and the number of hatched nymphs from oviposited eggs. Especially infected insects were sensitive to alternating temperature with low Causal virus of the rice dwarf disease was purified by an improved method. Purity was 3.24㎎/ml. Purified virus was injected to rabbit as an antigen and an antiserum with a titer of 1/4096 was obtained. When viruliferous vector and infected leaves were used as an antigen, detection of the virus was possible upto 1/8192 dilution by enzymelinked immunosorbent assay(ELISA) test. Seedlings of ninety lines of rice were inoculated for testing resistance. Eight lines including Milyang 30 were resistance, 23 lines including Nampung were moderate resistance and 60 lines including Kaya were susceptible. In mass screening of seedlings, of the 20 lines tested, Milyang 30 was resistance Naekyung and Nampung were moderate resistance and all the rest 17 lines were susceptible. In field screening, of the 64 lines tested, 20 lines including Milyang 30 were resistance, 15 lines including Baekyang were moderate resisance and 29 lines including Kaya were susceptible. temperature.