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합성배지에서 저온성 느타리(Pleurotus ostreatus 201)의 섬유소분해효소 생산조건
이극로,강춘기 한국식품영양학회 1993 韓國食品營養學會誌 Vol.6 No.2
합성배지에서 저온성 느타리(Pleurotus ostreatus 201)의 섬유소 분해효소 생산이 미치는 배양조건 및 탄소원의 영향을 검토하였다. 효소생산에 최적인 배양온도와 배지 pH는 avicelase가 25℃, 5.5이었고 CMCase는 30℃, 5.0 이었으며 β-glucosidase는 30℃, 6.5이었다. Avicelase와 CMCase 활성은 9일 배양하였을 때 최대치를 보였으며 β-glucosidase 활성은 18일 배양하였을 때가 가장 높았다. 탄소원 중에서 avicelase생산은 cellulose powder, CMCase생산은 Na-CMC, β-glucosidase생산은 cellobiose를 첨가하였을 때 가장 좋았다. 효소 생산은 cellulose powder 1.0%에서 가장 양호하였으며 glucose는 농도의 증가에 따라 현저하게 감소되었다. Cultural conditions and carbon sources affecting the productivity of cellulolytic enzymes of Pleurotus ostreatus 201 were examined in synthetic media. The optimum cultural temperature and initial pH for the production of enzymes were 25℃ and 5.5 in avicelase, and 30℃ and 5.0 in CMCase, and 30℃ and 6.5 in β-glucosidase. Among carbon sources, cellulose powder was the best for the production of avicelase, and Na-CMC for CMCase, and cellobiose for β-glucosidase. The optimum concentration of cellulose powder was 1.0%(w/v) , and glucose depressed the production of enzymes remarkably.
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한국산 표고버섯에서 분리한 Cysteine Sulfoxide Lyase 효소의 특성
이극로,김정숙 서강대학 지역발전연구소 1996 지역발전연구 : 서강전문대 Vol.2 No.-
Properties of cysteine sulfoxide lyase from Korean Lentinus edodes have been investigated. The optimum pH and temperature of cysteine sulfoxide lyase were the pH range of 8.0 to 9.0 at temperatures from 35 to 40℃. The enzyme were stable at temperatures lower than 40℃ and pH range of neutral to weakly alkaline pH. Des-glutamyl lentinic acid was the best substrate for cysteine sulfoxide lyase.
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합성배지에서 저온성 느타리 (Pleurotus ostreatus 201)의 섬유소 분해요소 생산
이극로 서강정보대학 1999 산업과학연구 Vol.1 No.-
합성배지에서 저온성 느타리( Pleurotus ostreatus 201) 의 섬유소 분해효소 생산에 미치는 질소원. 비타민 및 무기염류의 영향을 검토하였다. 효소 생산에 양호한 질소원은 urea이었고 아질산태질소는 효소생산을 억제하였으며 효소생산에 적당한 urea의 농도는 0.054%이었다. 효소 생산은 folic acid와 thiamine-HCl 에 의해 촉진되었고 KH₂PO₄와 MgSO₄의 최적 농도는 각각 0.2%, 0.04%이었으며 그 밖의 무기염류는 효과가 없었다. Nitrogen sources, vitamins and inorganic salts affecting the productivity of cellulolytic enzymes of pleurotus ostreatus 201 were examined in synthetic medium. Urea was a good nitrogen sources, while nitrite nitrogen depressed the production of enzymes. The appropriate concentration of urea was 0.054% (w/v). The enzymes production was stimulated by folic acid and thiamine-HCl. Among the inorganic salts, optimum concentration of KH₂PO₄, and MgSO₄ were 0.2% (w/v) and 0.04% respectively, but other inorganic salts were not effective on the production of enzymes.
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이극로 서강정보대학 1992 論文集 Vol.11 No.-
Compositions of free sugar and sugar alcohols, organic acids and fatty acids of Korean shiitake mushroom (Lentiuns edodes) were analyzed by gas liquid chromatography and compositional differences of these components depending on portions (pileus and stipe) were investigated. Glucose, fructose, trehalose, glycerol, arabitol and mannitol were identified and the major components were mannitol and arabitol, respectively. The total free sugar and sugar alcohol contents were 14.48g/100g(pileus), 17. 36g/100g(stipe). Total contents of these components were higher in stipe than those in pileus. Lactic, oxalic, fumaric, succinic, malic, citiric and pyroglutamic acid were identified and the major components were malic and citric acid, respectively. The total organic acid contents of these components were higher in pileus than those in stipes. The major fatty acids were linoleic, palmitic and oleic acid. Especially pentadecanoic, stearic and oleic acid were higher in stipe than in pileus and linoleic acid were higher in pileus than in stipe.
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