밤바구미(Curculio dentipes Roelofs)의 유충.용의 혈단백질에 관한 연구

이경로,신병식 한국곤충학회 1988 Korean journal of entomology Vol.18 No.2

밤바구미의 각 시기에 따른 혈단백질 패턴과 함량을 SDS-polyacrylamide gel electrophoresis법과 spectrophotometer를 사용하여 분리측정하였다. 1. 혈단백질 함량은 월동전 유충에서 최고의 함량을 나타내었다. 월동중 감소하다가 월동후 다시 증가하였으며 종령유충기부터 계속 감소하여 응시기에 최저 함량을 나타냈다. 2. 혈단백질 패턴은 전시기에 걸쳐 최고 230,200 daltons에서 최저 15,900 daltons까지 모두 44개의 밴드가 나타났으며 가장 염색강도가 강한 밴드는 분자량 77,900 doltons으로 모든 시기에서 나타났다. 특히 용시기에서 단백질의 변화가 크게 나타났다 The pattrens and concentration of hemolymph protein in the chestnut weevil, Curculio dentipes (Roelofs) according to development stages were determined by SDS-polyacrylamide gel electrophoresis and spectrophotometric method. The protein contents were found to be the maximum at the nonoverwintering larva and decreased at the wintering larva and the overwintering larva and then reached minimum level at pupa stages. The denatured proteins were separated into 44 bands between the highest M.W. 230,200 daltons and lowest M.W. 15,900 daltons during all stages. The band of M.W. 77,900 daltons appeared during all stages and showed greatest color density. Particulary, at overwintering larva and pupal stages, large variation were observed in protein bands.

한출변화과정(汗出變化過程)을 통(通)한 태음인(太陰人) 만성해수(慢性咳嗽) 치험례(治驗例)

이경로,이상기,송정모,Lee, Kyung-Ro,Lee, Sang-Gi,Song, Jung-Mo 사상체질의학회 2004 사상체질의학회지 Vol.16 No.3

1. Objectives In this study, it is experienced that the process of sweating is important in chronic cough of Taeumin. In this case report, the patient has been simproved throuth reaction of sweating improvement. In "Dongyi Soose Bowon", The process of sweating change is described in detail. "Hamkeylhae" is similar to chronic cough in "Dongyi Soose Bowon", it is cough that is hard to spit. The chronic cough of Taeumin is related to the pathology of Liver and Lung, sweating condition is also related to condition of Lung in Sasang constitutional medicine. So the study for the process of sweating is needed. 2. Methods One patient diagnosed as chronic cough was practiced with Sasang constitutional medicine. And We observed sweating change process during treatment. 3. Results As sweating condition improved, chronic cough also decreased, but not improved, not decreased. 4. Conclusions So treating of Taeumin's chronic cough is closely connected with sweating change process.

한국산 밤나무흑벌의 변태에 따른 지방산에 관한 연구

이경로 한국통합생물학회 1965 동물학회지 Vol.8 No.2

Fatty acid components of the Chestnut gall wasp (Dryocosmus kuriphilus YASUMATSU) were analyzed by the method of gas chromatogrphy at various developmental stages. 1. The fatty acids identified at each stage are as follows : Larva stage : acetic acid , benzoic acid and capric acid. Prepupa stage : acetic acid, benzoic acid and capric acid. Pupa stage : acetic acid, benzoic acid and capric acid. 2. Through the development of the wasp, there were large amount of acetic acid.

한국산 화랑곡 나방의 유리 아미노산에 관한 연구

이경로 한국통합생물학회 1964 동물학회지 Vol.7 No.1

The free amino acid content of Indian meal moth (Plodia interpunctella HUBNER) was analysed at various developmental stages by means of paper chromatography. 1) The free amino acids : present are alanine , arginine, aspartic acid, glutamic acid, glycine, histidine, leucine, methionine, proline, serine, threonine, tyrosine and valine. 2) Proline was detectable only in the acid-hydrolyzed Indian meal moth. 3) Arginine was clearly detected only in the larva stage. 4) Tyrosine methionine and valine were increased in the pupa stage. 5) Serine, glycine and tyrosine were present in high concentration in all stages.

솔잎혹파리 幼蟲의 血淋파 變化

李敬魯,李鍾鎭 건국대학교 1977 論文集 Vol.5 No.1

The concentration of free amino acids, total protein, trehalose, lipid, acid·alkaline phosphatase and glutamic oxaloacetic transaminase and glutamic pyruvic transaminase during the larval stage of the pine leaf gall midge, Thecodiploses japonensis Uchi. et Inouye were measured using Paper chromatographic method, micro-Kjeldahl method, Anthrone method, Thin layer chromatographic method, Bessey-Lowry method and Reitman-Franked method, respectively. Healthy specimens were chosen as samples of each developmental stages : the larve in gall, larva in soil and over wintered larva. Amino acids presentin the alcoholic extracts were alanine, aspartic acid, glutamic acid, glycine, histidine, methionine, proline, serine, threonine, tryptophan and valine. The total protein concentration reached to 195.925 mg/g during the larva in gall and the larval stage in soil of the value was decreased to 181.419 mg/g. In the over wintered larval stage the concentration reached a maximum that was value to 224.200 mg/g. The hemolymph sugar, trehalose value for over wintered larva was about two times the value for larva in gall. Total lipid, phospholipid, monoacylglycerol, triacylglycerol, sterol, free fatty acid and ester cholesterol were identified at each stages. Triacylglycerol concentration reached high level in contrast with other lipid contents during the larva in gall, larva in soil and over wintered larva. Monoacylglycerol, sterol and free fatty acid expert decal·eased lipid contents during at each stages. The activity of acid phosphatase generally decreased at larva in soil and increased at the over wintered larval stage. And in the throughout all stages there significant differences between both control and treatments group of acid allkaline phosphatase. The activities of glutamic oxaloacetic transaminase and glutamic pyruvic transaminase decreased gradually from larva in gall until the larva in soil and increased at over wintered larva stage. Hence, changes in free amino acids, total protein, acid phosphatase, glutamic oxaloacetic transaminase and glutamic pyruvic transaminase through the course of the physiological cycle form a U-shaped curve when graphed against the chemical composition and activity of hemolymph during the larva in gall, larva in soil and over wintered larva.

파노라마 이미지 기반 실내 장소 인식 실험 데이터세트 구축 및 검증 방법

이경로,이종훈 한국멀티미디어학회 2023 멀티미디어학회논문지 Vol.26 No.12

As interest increases in robotics, virtual reality, and indoor navigation systems, indoor location information extraction systems are becoming important. Among them, visual place recognition technology is being actively researched. Existing methods mainly use monocular images outdoors to recognize places. However, indoor monocular images alone provide insufficient information. Therefore, in this study, we build an indoor place recognition experimental data set based on panoramic images containing more information and propose a verification method. The proposed method showed better place recognition results when using panoramic images than monocular images. Therefore, this proposed method is expected to be helpful in developing an indoor location information extraction system and can be used in various fields.

『동의수세보원(東醫壽世保元』에 나타난 한열증(寒熱證)에 대한 고찰(考察)

이경로,김경요,Lee, Kyung-ro,Kim, Kyung-yoo 사상체질의학회 1999 사상체질의학회지 Vol.11 No.1

1. 목적 네 가지 유형의 사상체질에 대한 한열증을 알아보고, 이것이 각 체질에 대한 생리, 병리의 이해와 진단, 치료에 도움을 주고자 하였다. 2. 방법 동의수세보원에 나타난 생리, 병리와 몇 가지 증후를 통한 한열증의 개념과, 기존의학에서의 한열증의 개념을 고찰해 보았다. 3. 결과 및 결론 병증과 편명을 중심으로, 비신의 수곡출납의 과정에 따라, 소음인은 '이한'한 체질적 소인을 바탕으로 표열 이한증으로 소양인은 '이열'한 체질적 소인을 바탕으로 표한 이열증으로 나타난다. 한편, 간폐의 기액내외 호산에 따라, 태음인은 '소병한다자'와 '소병열다자'로 나누어지는 경향이 있으며, 태양인은 한증이나 열증으로 치우쳐진 경향이 아닌 '불한불열'의 특정을 나타낸다. 몇가지 증후(편비, 설사, 한출, 소변, 구토)를 중심으로 각 체질의 한열 속성에 따라 각 증후의 한열 개념이 정해지는 것을 알 수 있는데, 이러한 것은 '체질적' 변증 방법을 제시한 것으로 사료된다. 1. Purpose : This studied Cold and Heat syndromes about Four-form Constitutional Physicalogy. It is for help the comprehension, diagnosis and treatment of physicalogy and pathology about each type. 2. Method : I studied the conception of Cold and Heat sydrome in the existing Oriental Medicine before Constitutinal Medicine, through a few symptoms(constipation, diarrhea, sweat, urine, vomiting) and physicalogy, pathology in Dongyi-Soose-Bowon. 3. Results & Conclusion : In the viewpoint of Pathological Disease and a Chapter Name, Shaoyin has the Exterior-Febrile and Interior-Cold Disease, as the constitutional primary cause of the Interior-Cold Disease, Shaoyang has the Exterior-Cold and Interior-Febrile Disease, as the Interior-Febrile Disease, accoreing to the process of the promote incomings and outgoings of food stuffs of the Spleen and Kidney. By the way, Taiyin is classified into 'One who had had the Cold Disease Primarily and the Febrile', Taiyang has the nature of 'No Cold, No Heat' that is not tendency to The Cold or Heat sydromes, according to promote the respiration of air and fluid of the Liver and Lung. In the viewpoint of a few symptoms(constipation, diarrhea, sweat, urine, vomiting), we are known that cold and heat connception of the each symptome is fixed, according to nature of cold and heat in each type. These express the methood of 'constitutional demonstration'.

먼지시료채취 숙련도 시험 점수 분포에 따른 시험소별 부적합 사례 분석

이경로,고상호,김지혜,전수아,이진주,이혜리,황종연,최종우 환경독성보건학회 2019 한국독성학회 심포지움 및 학술발표회 Vol.2019 No.10

솔잎혹파리의 변태에 따른 용기의 체액분석

이경로,이종진,이상석,고진복 한국곤충학회 1982 Korean journal of entomology Vol.12 No.2

The concetration of total protein and non protein nitrogen, lipid, trehalose, acid$.$alkaline phosphatase, glutamic oxaloacetic transaminase, glutamic pyruvic transaminase and mineral salts during the pupal stage in the pine leaf gall midge, Thecodiptosis japonensis Uchida et Inouye, were measured by using micro-Kjeldahl method, Thin layer chromatographic method, Anthrone method, Reitman-Frankel method, Oser method and EEL dame photometric method respectively. Healthy specimens were chosen as samples of each developmental stages; the prepupa and the late pupa. The concentration of total protein was 88.139mg/g in the prepupa and was 120.719mg/g in the late pupal stage. Non protein nitrogen contents was 2.292mg/g in the prepupa add was 4.225mg/g in the late pupal stage. In the late pupal stage total protein and non protein nit-rogen contents was higher than those in the prepupal stage. Total lipid, phospholipid, menoacylgly cerol, free fatty acid, sterol and ester cholesterol were identified in the prepupa and the late pupal stage. The concentration of total lipid was 150.0mg/g in the prepupa and was 162.8mg/g in the late pupal stage. With the exception of total lipid, phospholipid, monoacylglycerol, free fatty acid, sterol and ester cholesterol contents were higher than in the late pupa those in the prepupal stage. Rf value of trehalose analyzed on thin layer chromatogram was 0.714 in both stages, and the concentration of trehalose was 3.640mg/g in the Prepupa and was 3.553mg/g in the late pupal stage. The activity of acid phosphatase was 0.272unit/mg in the prepupal and was 0.281unit/mg in the late pupal stage, and alkaline phosphatase was 0.413unit/mg in the prepupa and was 0.546unit/mg in the late pupal stage. The activities of glutamic oxaloacetic transaminase and glutamic pyruvic transaminase showed the same patterns wi th acid . alkaline phosphatase. Mineral salts present were calcium, phosphorus, sodium and potassium, which were identified in both stages. All kinds of mineral salts contents in the late pupa were higher than those in the prepupal stage. In view of controlling pest, the patterns of the physiological activties indicate that control will be the most effective at the time of the worst physiological manifested at the prepupal stage. 솔잎혹파리 (Thecodiplosis japonensis Uchida et Inouye)의 변태기 중 전용과 후용의 액체 변화를 분석하고 그 활성을 측정코자 micro-Kjeldahl법, Thin layer chromatography법, Anthrone법, Reitman-Fra-nkel법, Oser법, EEL flame photometer법으로 측정분석하였다. 1)전용과 후용에서 총단백질과 비단백성질소, 지질, 탄수화물, phosphatase, GOT, GPT, 무기염류의 함량과 화성도가 측정되었다. 2) 총단백질과 비단백성질소 모두 후용에서 높은 함량의 변화를 나타냈다. 3) 분석된 지질은 총지질, 인지질, monoacylalycerol, 유리지방산, sterol, triacylglycerol, ester cholesterol이였으며 triacylglycerol을 제외하고 후용에서 각 지질의 함량이 전용에서 보다 높았다. 4) trehalose의 함량은 큰 차이는 없었으나 전용에서 다소 높았다. 5) 효소 활성도의 변화는 acid.alkaline phosphatase, GOT, GPT 모두 후용에서 높았으며, GOT는 GPT에 비해 매우 높은 활성을 보였다. 6) 무기염류는 칼슘, 인, 나트륨, 칼륨이 분석되었으며, 칼륨이 가장 높은 함량을 나타냈다. 7) 전용과 후용의 생리적 활성을 비교하면 전용기가 생리적 기능하강기로 나타나 이 시기가 생물학적 방제의 최적기로 이용될 수 있을 것이다.

밤나무혹벌의 신생태종에 관한 연구(II)

이경로,이종진,신병식 한국곤충학회 1985 Korean journal of entomology Vol.15 No.2

재래종 및 저항성 밤나무에 기생하는 밤나무혹벌의 전 변태기 동안에 단백질의 패턴과 분자량을 SDS-polyacrylamide gel electrophoresis법을 이용하여 측정하였다. 1. 각 변태기중 유충기와 성충기 보다 용시기에서 더많은 단백질 밴드가 분리되었고, 같은 시기에 고분자단백 질의 밴드수가 증가되었다. 2. 전 변태단계에서 분자량 약 90,000~80,000과 50,000~40,000 부위가 2~3개의 주된 단백질 밴드로 분리되었다. 3. 재래종 밤나무에 기생하는 밤나무혹벌의 성충기에서는 다른 두 저항성 밤나무에 기생하는 밤나무혹벌 보다 더 많은 밴드가 분리되었으나, 밴드의 농도는 낮아서 다른 것과 현저한 차이가 나타났다. 4. 유충기의 경우, 재래 종은 축파와 단택에 기생하는 밤나무혹벌의 복합된 단백질 패턴을 보였다. 5. 밤나무혹벌간의 단백질 패턴의 차이는 기주식물에 따른 새로운 밤나무혹벌의 출현을 시사하며, 이 사실은 유전적 변이와 신생태종을 확인하는 기초자료가 된다. During metamorphosis, the protein patterns and major proteins molecular weight of chestnut gall wasp, Dryocosnus kuriphilus Yatsumatsu parasiting in susceptible chestnut and resistant chestnut were determinded by SDS-polyacrylamide gel electrophoresis. During pupal stage, more protein bands than those of the larval stages and adult stages were separated and the number of high molecular weight protein bands was increased at the same stage. Two or three major protein bands were distributed, whose molecular weights are about 80,000 to 90,000 and 40,000 to 50,000 at all developmental stages. A larger number of protein bands than those of two resistant strains were separated at the adult stage of susceptible strain and these protein bands showed remarkable differentation owing to lower color density. In case of larval stages, the susceptible strain showed the pattern of protein mixed with Cheuk Pa ana Dan Tak. It is conspicuous that changes of protein pattern of D. kuriphilus according to the host plants are caused by genetic variation and will he give the basal data in relation to the ecospecies.