밤바구미(Curculio dentipes Roelofs)의 유충.용의 혈단백질에 관한 연구

이경로,신병식 한국곤충학회 1988 Korean journal of entomology Vol.18 No.2

밤바구미의 각 시기에 따른 혈단백질 패턴과 함량을 SDS-polyacrylamide gel electrophoresis법과 spectrophotometer를 사용하여 분리측정하였다. 1. 혈단백질 함량은 월동전 유충에서 최고의 함량을 나타내었다. 월동중 감소하다가 월동후 다시 증가하였으며 종령유충기부터 계속 감소하여 응시기에 최저 함량을 나타냈다. 2. 혈단백질 패턴은 전시기에 걸쳐 최고 230,200 daltons에서 최저 15,900 daltons까지 모두 44개의 밴드가 나타났으며 가장 염색강도가 강한 밴드는 분자량 77,900 doltons으로 모든 시기에서 나타났다. 특히 용시기에서 단백질의 변화가 크게 나타났다 The pattrens and concentration of hemolymph protein in the chestnut weevil, Curculio dentipes (Roelofs) according to development stages were determined by SDS-polyacrylamide gel electrophoresis and spectrophotometric method. The protein contents were found to be the maximum at the nonoverwintering larva and decreased at the wintering larva and the overwintering larva and then reached minimum level at pupa stages. The denatured proteins were separated into 44 bands between the highest M.W. 230,200 daltons and lowest M.W. 15,900 daltons during all stages. The band of M.W. 77,900 daltons appeared during all stages and showed greatest color density. Particulary, at overwintering larva and pupal stages, large variation were observed in protein bands.

『동의수세보원(東醫壽世保元』에 나타난 한열증(寒熱證)에 대한 고찰(考察)

이경로,김경요,Lee, Kyung-ro,Kim, Kyung-yoo 사상체질의학회 1999 사상체질의학회지 Vol.11 No.1

1. 목적 네 가지 유형의 사상체질에 대한 한열증을 알아보고, 이것이 각 체질에 대한 생리, 병리의 이해와 진단, 치료에 도움을 주고자 하였다. 2. 방법 동의수세보원에 나타난 생리, 병리와 몇 가지 증후를 통한 한열증의 개념과, 기존의학에서의 한열증의 개념을 고찰해 보았다. 3. 결과 및 결론 병증과 편명을 중심으로, 비신의 수곡출납의 과정에 따라, 소음인은 '이한'한 체질적 소인을 바탕으로 표열 이한증으로 소양인은 '이열'한 체질적 소인을 바탕으로 표한 이열증으로 나타난다. 한편, 간폐의 기액내외 호산에 따라, 태음인은 '소병한다자'와 '소병열다자'로 나누어지는 경향이 있으며, 태양인은 한증이나 열증으로 치우쳐진 경향이 아닌 '불한불열'의 특정을 나타낸다. 몇가지 증후(편비, 설사, 한출, 소변, 구토)를 중심으로 각 체질의 한열 속성에 따라 각 증후의 한열 개념이 정해지는 것을 알 수 있는데, 이러한 것은 '체질적' 변증 방법을 제시한 것으로 사료된다. 1. Purpose : This studied Cold and Heat syndromes about Four-form Constitutional Physicalogy. It is for help the comprehension, diagnosis and treatment of physicalogy and pathology about each type. 2. Method : I studied the conception of Cold and Heat sydrome in the existing Oriental Medicine before Constitutinal Medicine, through a few symptoms(constipation, diarrhea, sweat, urine, vomiting) and physicalogy, pathology in Dongyi-Soose-Bowon. 3. Results & Conclusion : In the viewpoint of Pathological Disease and a Chapter Name, Shaoyin has the Exterior-Febrile and Interior-Cold Disease, as the constitutional primary cause of the Interior-Cold Disease, Shaoyang has the Exterior-Cold and Interior-Febrile Disease, as the Interior-Febrile Disease, accoreing to the process of the promote incomings and outgoings of food stuffs of the Spleen and Kidney. By the way, Taiyin is classified into 'One who had had the Cold Disease Primarily and the Febrile', Taiyang has the nature of 'No Cold, No Heat' that is not tendency to The Cold or Heat sydromes, according to promote the respiration of air and fluid of the Liver and Lung. In the viewpoint of a few symptoms(constipation, diarrhea, sweat, urine, vomiting), we are known that cold and heat connception of the each symptome is fixed, according to nature of cold and heat in each type. These express the methood of 'constitutional demonstration'.

한출변화과정(汗出變化過程)을 통(通)한 태음인(太陰人) 만성해수(慢性咳嗽) 치험례(治驗例)

이경로,이상기,송정모,Lee, Kyung-Ro,Lee, Sang-Gi,Song, Jung-Mo 사상체질의학회 2004 사상체질의학회지 Vol.16 No.3

1. Objectives In this study, it is experienced that the process of sweating is important in chronic cough of Taeumin. In this case report, the patient has been simproved throuth reaction of sweating improvement. In "Dongyi Soose Bowon", The process of sweating change is described in detail. "Hamkeylhae" is similar to chronic cough in "Dongyi Soose Bowon", it is cough that is hard to spit. The chronic cough of Taeumin is related to the pathology of Liver and Lung, sweating condition is also related to condition of Lung in Sasang constitutional medicine. So the study for the process of sweating is needed. 2. Methods One patient diagnosed as chronic cough was practiced with Sasang constitutional medicine. And We observed sweating change process during treatment. 3. Results As sweating condition improved, chronic cough also decreased, but not improved, not decreased. 4. Conclusions So treating of Taeumin's chronic cough is closely connected with sweating change process.

솔잎혹파리의 변태에 따른 용기의 체액분석

이경로,이종진,이상석,고진복 한국곤충학회 1982 Korean journal of entomology Vol.12 No.2

The concetration of total protein and non protein nitrogen, lipid, trehalose, acid$.$alkaline phosphatase, glutamic oxaloacetic transaminase, glutamic pyruvic transaminase and mineral salts during the pupal stage in the pine leaf gall midge, Thecodiptosis japonensis Uchida et Inouye, were measured by using micro-Kjeldahl method, Thin layer chromatographic method, Anthrone method, Reitman-Frankel method, Oser method and EEL dame photometric method respectively. Healthy specimens were chosen as samples of each developmental stages; the prepupa and the late pupa. The concentration of total protein was 88.139mg/g in the prepupa and was 120.719mg/g in the late pupal stage. Non protein nitrogen contents was 2.292mg/g in the prepupa add was 4.225mg/g in the late pupal stage. In the late pupal stage total protein and non protein nit-rogen contents was higher than those in the prepupal stage. Total lipid, phospholipid, menoacylgly cerol, free fatty acid, sterol and ester cholesterol were identified in the prepupa and the late pupal stage. The concentration of total lipid was 150.0mg/g in the prepupa and was 162.8mg/g in the late pupal stage. With the exception of total lipid, phospholipid, monoacylglycerol, free fatty acid, sterol and ester cholesterol contents were higher than in the late pupa those in the prepupal stage. Rf value of trehalose analyzed on thin layer chromatogram was 0.714 in both stages, and the concentration of trehalose was 3.640mg/g in the Prepupa and was 3.553mg/g in the late pupal stage. The activity of acid phosphatase was 0.272unit/mg in the prepupal and was 0.281unit/mg in the late pupal stage, and alkaline phosphatase was 0.413unit/mg in the prepupa and was 0.546unit/mg in the late pupal stage. The activities of glutamic oxaloacetic transaminase and glutamic pyruvic transaminase showed the same patterns wi th acid . alkaline phosphatase. Mineral salts present were calcium, phosphorus, sodium and potassium, which were identified in both stages. All kinds of mineral salts contents in the late pupa were higher than those in the prepupal stage. In view of controlling pest, the patterns of the physiological activties indicate that control will be the most effective at the time of the worst physiological manifested at the prepupal stage. 솔잎혹파리 (Thecodiplosis japonensis Uchida et Inouye)의 변태기 중 전용과 후용의 액체 변화를 분석하고 그 활성을 측정코자 micro-Kjeldahl법, Thin layer chromatography법, Anthrone법, Reitman-Fra-nkel법, Oser법, EEL flame photometer법으로 측정분석하였다. 1)전용과 후용에서 총단백질과 비단백성질소, 지질, 탄수화물, phosphatase, GOT, GPT, 무기염류의 함량과 화성도가 측정되었다. 2) 총단백질과 비단백성질소 모두 후용에서 높은 함량의 변화를 나타냈다. 3) 분석된 지질은 총지질, 인지질, monoacylalycerol, 유리지방산, sterol, triacylglycerol, ester cholesterol이였으며 triacylglycerol을 제외하고 후용에서 각 지질의 함량이 전용에서 보다 높았다. 4) trehalose의 함량은 큰 차이는 없었으나 전용에서 다소 높았다. 5) 효소 활성도의 변화는 acid.alkaline phosphatase, GOT, GPT 모두 후용에서 높았으며, GOT는 GPT에 비해 매우 높은 활성을 보였다. 6) 무기염류는 칼슘, 인, 나트륨, 칼륨이 분석되었으며, 칼륨이 가장 높은 함량을 나타냈다. 7) 전용과 후용의 생리적 활성을 비교하면 전용기가 생리적 기능하강기로 나타나 이 시기가 생물학적 방제의 최적기로 이용될 수 있을 것이다.

한국산 밤나무흑벌의 변태에 따른 지방산에 관한 연구

이경로 한국통합생물학회 1965 동물학회지 Vol.8 No.2

Fatty acid components of the Chestnut gall wasp (Dryocosmus kuriphilus YASUMATSU) were analyzed by the method of gas chromatogrphy at various developmental stages. 1. The fatty acids identified at each stage are as follows : Larva stage : acetic acid , benzoic acid and capric acid. Prepupa stage : acetic acid, benzoic acid and capric acid. Pupa stage : acetic acid, benzoic acid and capric acid. 2. Through the development of the wasp, there were large amount of acetic acid.

솔잎혹파리 幼蟲의 血淋파 變化

李敬魯,李鍾鎭 건국대학교 1977 論文集 Vol.5 No.1

The concentration of free amino acids, total protein, trehalose, lipid, acid·alkaline phosphatase and glutamic oxaloacetic transaminase and glutamic pyruvic transaminase during the larval stage of the pine leaf gall midge, Thecodiploses japonensis Uchi. et Inouye were measured using Paper chromatographic method, micro-Kjeldahl method, Anthrone method, Thin layer chromatographic method, Bessey-Lowry method and Reitman-Franked method, respectively. Healthy specimens were chosen as samples of each developmental stages : the larve in gall, larva in soil and over wintered larva. Amino acids presentin the alcoholic extracts were alanine, aspartic acid, glutamic acid, glycine, histidine, methionine, proline, serine, threonine, tryptophan and valine. The total protein concentration reached to 195.925 mg/g during the larva in gall and the larval stage in soil of the value was decreased to 181.419 mg/g. In the over wintered larval stage the concentration reached a maximum that was value to 224.200 mg/g. The hemolymph sugar, trehalose value for over wintered larva was about two times the value for larva in gall. Total lipid, phospholipid, monoacylglycerol, triacylglycerol, sterol, free fatty acid and ester cholesterol were identified at each stages. Triacylglycerol concentration reached high level in contrast with other lipid contents during the larva in gall, larva in soil and over wintered larva. Monoacylglycerol, sterol and free fatty acid expert decal·eased lipid contents during at each stages. The activity of acid phosphatase generally decreased at larva in soil and increased at the over wintered larval stage. And in the throughout all stages there significant differences between both control and treatments group of acid allkaline phosphatase. The activities of glutamic oxaloacetic transaminase and glutamic pyruvic transaminase decreased gradually from larva in gall until the larva in soil and increased at over wintered larva stage. Hence, changes in free amino acids, total protein, acid phosphatase, glutamic oxaloacetic transaminase and glutamic pyruvic transaminase through the course of the physiological cycle form a U-shaped curve when graphed against the chemical composition and activity of hemolymph during the larva in gall, larva in soil and over wintered larva.

Methoprene 처리에 따른 매미나방(Lymantria dispar L.) 유충의 중장상피와 혈림프단백질 함량

박혜숙,이경로 建國大學校基礎科學硏究所 1991 理學論集 Vol.16 No.-

매미나방(Lymantria dispar L.) 유충에 JH mimics인 methoprene을 처리하여 형태학적·생리학적 영향을 조사한 결과는 다음과 같다. 중장상피의 형태학적인 변화는 대조군과 처리군 유충의 원주세포(columnar cell)와 배상세포(goblet cell)에서 관찰하였다. 처리군의 원주세포는 지질성 과립수가 현저하게 증가하였고 핵은 커지고 조면소포체(rough endoplasmic reticulum)는 원형으로 팽대되였다. 세포표면의 미세융모(microvilli)는 거의 영향을 받지 않았다. 미토콘드리아가 내재된 배상세포의 세포질 돌출부는 거의 해체되고 세포질성의 많은 조각(또는 swirled membrane bodies)들이 배상세포의 내강(goblet cavity)으로 배출되었다. 생리학적인 변화는 대조군과 처리군에서 혈림프단백질 함량을 측정하여 비교하였다. 혈림프단백질의 함량 변화는 대조군과 처리군에서 암·수 모두 유사한 양상을 나타내었다. 그러나 전반적인 함량의 변화는 처리군이 대조군에서 보다 낮은 경향을 나타냈다. 또한 methoprene을 처리한 유충은 그 유충기간이 정상 발생군보다 연장되었으며 7령의 수컷이 유도되었다. The morphological and physiological effects of the juvenile hormone mimics, methoprene on the larvae of Lymantria dispar were investigated. Morphological changes were observed in the columnar and goblet cells in the mid gut of the treated Lymantria dispar larvae. In the columnar cells, the amount of lipid droplets were extremely increased and the nucleus was enlarged and the endoplasmic reticulum assumed the vaculoe-like configuration. Microvilli on the cell surface, however, were mostly preserved intact. In the goblet cells, the mitochondria-filled cytoplasmic projections were completely disorganized and the numerous fragments(or swirled membrane bodies) of plasma membrane were discharged into the goblet cavity. The protein concentrations of hemolymph of the normal and treated larvae were measured to determine the physiological effects of methoprene. The similar pattern of changes in protein concentrations of hemolymph was observed throughout the developments of the male and female larvae of two groups. The overall protein concentration of the treated larvae was lower than that of the normal larvae. It was found that the methoprene prolonged the larval stage and induced the male 7th instar larvae.

한국산 화랑곡 나방의 유리 아미노산에 관한 연구

이경로 한국통합생물학회 1964 동물학회지 Vol.7 No.1

The free amino acid content of Indian meal moth (Plodia interpunctella HUBNER) was analysed at various developmental stages by means of paper chromatography. 1) The free amino acids : present are alanine , arginine, aspartic acid, glutamic acid, glycine, histidine, leucine, methionine, proline, serine, threonine, tyrosine and valine. 2) Proline was detectable only in the acid-hydrolyzed Indian meal moth. 3) Arginine was clearly detected only in the larva stage. 4) Tyrosine methionine and valine were increased in the pupa stage. 5) Serine, glycine and tyrosine were present in high concentration in all stages.

매미나방(Lymantria dispar) 유충의 혈림프 유리아미노산의 함량 변화

이경로,강정호,이해풍 한국곤충학회 1990 Entomological Research Vol.20 No.1

매미나방(Lymantria dispar) 유충의 발생에 따른 혈림프 유리아미노산을 HPLC를 사용하여 분석하였다. 유충의 혈림프에서 필수아미노산을 포함한 18종의 유리아미노산을 분리하였고, aspartic acid는 특이하게 3령 유충에서만 검출되었다. 전 발생단계에서 histidine이 최고함량을 나타냈고 다음으로 arginine과 lysine이 높게 나타났다. 영별 아미노산의 함량변화는 수컷 3령 유충에서 가장 높게 나타난 후 계속 감소하였으며, 암컷은 4령 유충에서 최고치를 보였고 감소하다가 7령 유충에서 다시 증가하였다. 한편, 총함량은 암컷이 수컷에서 보다 높게 나타났다. 일별 아미노산도 양적 변화를 보여 histidine이 전시기에 걸쳐 50% 이상이었고, arginine, lysine, serine등도 비교적 높았다. 일별 변화에서 총함량은 암 수 모두 5령 유충의 3일에서 가장 높게 나타났다. 수컷은 1일에서 최저치였으나 암컷은 2일과 5일에서 거의 비슷한 수준이었으며, 성별 비교에서는 암컷이 높아 영별 변화와 일치하였다. Free amino acids in the hemolymph during larva stages of the gypsy moth, Lymantria dispar were determined by HPLC. A total of 18 free amino acids including 10 essential amino acids were found but aspartic acid was present at 3rd instar larva only. Histidine was predominant amino acids, then arginine and lysine were shown to have high concentration at all stages. In male the concentration of total free amino acids decreased gradually from 3rd to 6th instar larva. At the 4th female larva, that of total free amino acids marked maximum. But it declined rapidly to 6th instar larva, and then followed by increase at 7th instar larval stage. During 5th instar larva development, free amino acids also showed changes in concentration 3 old day larva contained the highest content at both sexes. 1 old day male larva appeared the minimum level, whereas 2 old and 5 old day larvae marked low level at female.

밤바구미(Curculio dentipes) 월동 중 휴면 유충의 Ecdysteroid의 농도 변화

유진수,이충언,이경로 建國大學校基礎科學硏究所 1996 理學論集 Vol.21 No.-

월동 중인 밤바구미(Curculio dentipes) 휴면 유충의 발생에 따른 ecdysone과 20-hydroxyecdysone의 농도를 high performance liquid chromatography(HPLC)를 이용하여 측정하였다. Ecdysone 농도는 pre-diapausing larvae(19.81ng/g)에서 최저치를 나타내었고, diapausing larvae(29.97ng/g)에서 최대를 보였으며, post-diapausing larvae(25.71ng/g)에서 약간 감소하였다. 그러나 20-hydroxyecdysone은 pre-diapausing larvae(29.29ng/g)에서 가장 높은 농도를 보인 후, diapausing larvae(5.05ng/g)에서 급격히 감소하였고, post-diapausing larvae(12.53ng/g)에서 다시 증가하는 경향을 보여 생리기능상 역관계를 나타내었다. The concentration of ecdysteroid was measured in the pre-diapausing, diapausing, and post-diapausing larvae of the chestnut weevil. Curculio dentipes. Ecdysteroid titers were measured by high performance liquid chromatography(HPLC) during developmental stages. The concentration of ecdysone was the minimum(19.81ng/g) at pre-diapausing larvae and the maximum(29.97ng/g) at diapausing larvae, and it decreased to 25.71ng/g at post-diapausing larvae, and then kept relatively at high level. But the amount of 20-hydroxyecdysone was the highest value at pre-diapausing larvae(29.29ng/g) and minimum(5.05ng/g) at diapausing larvae, but it increased to the 12.53ng/g at post-diapausing larvae. In conclusion, the relationship between ecdysone and 20-hydroxyecdysone titer was shown to be inverse.