토복령(土茯笭)이 RAW264.7 대식세포주에서 산화질소 억제에 미치는 영향

이강희,정준희,김이화,이재혁,Lee, Kang-Hee,Jung, Jun-Hee,Kim, Ee-Hwa,Lee, Jae-Hyuk Society for Meridian and Acupoint 2009 Korean Journal of Acupuncture Vol.22 No.2

Objectives : 본 연구의 목적은 제습, 해독, 통리관절등의 효능이 있는 토복령이 RAW264.7 대식세포주에서 lipopolysaccharide(LPS)로 처치하여 생성되는 nitric oxide(NO)와 prostaglandin $E_2$($PGE_2$)에 억제작용이 있는지 관찰하고자 하는 것이다. Methods : 토복령이 RAW264.7 대식세포주에 세포독성이 유무를 관찰하기 위하여 농도별 MTT assay를 수행하여 세포생존율을 측정하였다. 또한 LPS로 염증유발된 RAW264.7 대식세포주에서 토복령의 농도별 처치가 NO 및 $PGE_2$ 생성억제에 미치는 영향을 관찰하고자 NO 및 $PGE_2$ assay를 수행하였다. Results : 토복령의 농도별 처치가 RAW264.7 대식세포주에서 세포독성을 유발하는지 MTT assay로 측정한 결과 세포독성은 관찰되지 않았으며, 토복령은 LPS처치로 인하여 증가된 NO 및 $PGE_2$ 생성을 통계학적으로 유의하게 감소시킴을 관찰하였다. Conclusions : 본 연구를 통하여 토복령은 RAW264.7 대식세포주에서 LPS로 유도된NO 및 $PGE_2$ 생성을 효과적으로 억제시킴으로써 추후 염증질환의 치료제로서 가능성을 확인하였다.

양성자 주입 기술을 이용한 초고속 회복 다이오드의 제작

이강희,김병길,이용현,백종무,이재성,배영호,Lee, Kang-Hee,Kim, Byoung-Gil,Lee, Yong-Hyun,Baek, Jong-Mu,Lee, Jae-Sung,Bae, Young-Ho 한국전기전자재료학회 2004 전기전자재료학회논문지 Vol.17 No.12

Proton irradiation technology was used for the improvement of power diode switching characteristics. Proton irradiation was carried out at the energies of 2.32 MeV, 2.55 MeV, 2.97 MeV so that the projection range of irradiated proton would be at the metallurgical junction, depletion region and neutral region of pn diode, respectively. Dose conditions were varied into three conditions of 1${\times}$10$^{11}$ cm$^{-2}$ , 1${\times}$10$^{12}$ cm$^{-2}$ , 1${\times}$10$^{13}$ cm$^{-2}$ at each condition of energies. Characterization of the device was performed by I-V(current-voltage), C-V(capacitance-voltage) and trr(reverse recovery time) measurement. At the optimum condition of irradiation, the reverse recovery time of device has been reduced about 1/5 compared to that of original un-irradiated device.

공동주택의 관리비 증감특성 연구

이강희,채창우,박근수,Lee, Kang-Hee,Chae, Chang-U,Park, Guen-Soo 한국주거학회 2011 한국주거학회 논문집 Vol.22 No.6

The maintenance cost depends on various factors such as building volume, floor area, number of household and so on. The maintenance cost of the apartment housing is affected by the maintenance type, building physical factor, sociogeographic aspects. Among these, the maintenance characteristics is represented and made up by the total floor area and number of household which means main factor to provide the building scale roughly. In this paper, it aimed at modelling the estimation function of the maintenance cost with the total floor area and number of household and analyzing the elasticity of the two factors. Although items of maintenance cost are various in general cost, repair cost and so on, we classified these items into the 5 categories. 5 categories are a general cost, a facility maintenance cost, a utilization cost, insurance and sanitary cost. The estimation function used a power function and it has better goodness-offitness than any other estimation methods in statistics. A power function has a three curve types with concave and convex and linear style to the origin.

Electrochemical Machining of Tungsten Carbide Microshaft

이강희,Lee, Kang-Hee The Korean Society of Manufacturing Technology Eng 2010 한국공작기계학회지 Vol.19 No.3

본 연구에서는 전해가공을 이용해서 직경이 균일한 텅스텐 카바이드 미세축을 제작하는 실험을 수행하였다. 전해가공을 통해 미세축으로 사용 가능한 형상을 얻기 위한 최적의 가공 조건에 대해 고찰하였다 이 과정에서 미세축의 형상에 영향을 주는 여러 인자들을 적절하게 조절하여 최적의 형상을 얻을 수 있었다. 그리고 가공된 미세축을 이용하여 적절한 조건으로 2차, 3차 가공을 수행하여 초미세축을 가공할 수 있음을 보였다. 그리하여 실험 결과 직경 $30{\mu}m$, 길이 $500{\mu}m$의 텅스텐 카바이드 미세축을 제작하였다. Tungsten carbide microshaft is used as micro punch, electrode of micro electro discharge machining, and micro tool because of its high hardness and rigidity. In this research, tungsten carbide microshaft was fabricated using electrochemical machining. $H_2SO_4$ solution was used as the electrolyte because it can dissolve tungsten carbide and cobalt simultaneously. Experimentally studied were the effects of electrolyte concentration, machining time, and machining voltage on material removal rate and the shape of the microshaft. To eliminate the effects of bubbles and metal corrosion layer on microshaft shape, the machining was performed below the electrolysis voltage. Three step electrochemical process was suggested to fabricate the straight tungsten carbide microshaft. As a result, a straight tungsten carbide microshaft of $30{\mu}m$ in diameter and $500{\mu}m$ in length was obtained through the proposed three step electrochemical process.

교육시설 내용년한 산정 연구 - 옥상방수와 바닥마감재를 대상으로 -

이강희,채창우,Lee, Kang-Hee,Chae, Chang-U 한국교육시설학회 2017 敎育施設 Vol.24 No.6

Educational facilities have an affect to make a decently learning environment. After constructed, it needs a maintenance plan to keep the performance or function which provide the repair time, repair scope and ratio. But the fundamental data are so insufficient that the field worker can't provide the maintenance plan and has no choice use the other data which concerned with apartment or office building. Above all, the service life is indispensible to make a repair plan because the repair time and scope would be provided within the service life. This study aimed at providing the method to make a service life of component in educational facilities and applying the method into the roof proof and floor finishing. Results are shown that first, it is important to set the $1^{st}$ repair time after constructed. when it proposes the three ways with the probability approach, choice probability model and cumulative cost function. Second, the service life of roof proof is provided with about 35 years. In addition, the service life of the floor finishing is about 40 years. These result would be utilized to conduct the repair plan under the service life.

지지점 간극을 갖는 다점지지 유연관의 유동하중에 의한 시간응답 이력해석과 상용유한요소 해석코드의 적용

이강희,강흥석,신창환,Lee, Kang Hee,Kang, Heung Seok,Shin, Chang Hwan 한국압력기기공학회 2015 한국압력기기공학회 논문집 Vol.11 No.2

Time domain response analysis for vibro-impact nonlinear behavior of multi-span tube with loose supports was performed using commercial FEA code and user subroutine. Support geometry of multi-span tube with a finite gap is realistically modeled by analytical rigid surface. Model of hydrodynamic force is based on the Qusai-steady model which accounts for the inclined angle of relative flow velocity and time delay between flow force and resulting tube motion. During tube vibration from flow loading, impact and friction at the support location is simulated using commercial FEA code with master slave contact algorithm. Analysis results has reasonable agreement with those of references and test experience. Plan of further refinement of analysis model and future test verification is briefly introduced.

공동주택 공종별 수선시기 설정 연구

이강희,박근수,채창우,Lee, Kang-Hee,Park, Geun-Soo,Chae, Chang-U 한국주거학회 2011 한국주거학회 논문집 Vol.22 No.4

Apartment is settled down as a main housing type and massive supply has been conducted since 1990's. The government has focused on the housing supply to improve the housing number and cut its price. On the contrary, the management of the housing has been neglected to get an early deterioration. Since 1995's, various ways have been made to improve and maintain the building function and performance. Among these, the long-term repair plan has played a main role to manage the housing condition and plan the repair schedule for preparing the massive cost of repair such as a roof proof, elevator change. The long-tern repair plan provides the repair time and repair rate according to various construction types. Most of the management agent apply the repair standard into the field area. But the repair time could not reflect the present condition and bring into the wrong maintenance. Therefore, the repair time for the long-term maintenance plan would be revised and reflected from the material and technology development. |n this study, it aimed at improvement for the repair time in various construction types which are provided in the long-term repair plan. For this, the 22 management agents are surveyed directly. This study shows that the repair time of the painting are correspondent to the repair time of the long-term plan irregardless of the painting materials. Most of other areas would correspondently similar to the long-term plan. This study could not include the building location, material and other haracteristics. Therefore, the further study would be needed to reflect the building and materials attributes to get the detailed repair time.

강화지역 청소년의 4년간 혈청 지질의 변화와 지속성

이강희,서일,지선하,남정모,김성순,심원흠,하종원,김석일,강형곤,Lee, Kang-Hee,Suh, Il,Jee, Sun-Ha,Nam, Chung-Mo,Kim, Sung-Soon,Shim, Won-Heum,Ha, Jong-Won,Kim, Suk-Il,Kang, Hyung-Gon 대한예방의학회 1997 Journal of Preventive Medicine and Public Health Vol.30 No.1

소아에서부터 청소년기까지 혈청 지질의 지속성(tracking)은 서구에서 행해진 연구들에 의해서 이미 잘 알려져 있으나 국내에서는 이에 관한 연구가 거의 이루어지지 않았다. 본 연구는 우리나라 청소년의 혈청 지질치의 변화를 조사하고 지속성이 있는지를 분석하였다. 연구대상은 경기도 강화군에서 진행하고 있는 강화 아동 혈압 코호트(719명)의 청소년들로 12세, 14세, 16세 세 번에 걸쳐서 공복 후 혈액검사(혈청 총 콜레스테롤, 중성지방, LDL 콜레스테롤, HDL 콜레스테롤을 포함)와 신체계측을 시행하였다. 이 중 3번의 혈액 검사에서 10시간 이상의 공복이 확인된 청소년 309명 (남자 162명, 여자 147명)을 최종 연구대상으로 선정하였다. 연구대상이 된 청소년들과 연구대상에서 제외한 청소년들의 12세때 초기 검사치들을 t 검정을 이용하여 서로 비교한 결과 통계학적으로 유의한 차이는 없었다. 연구방법은 우선 혈청 지질치의 변화양상을 보기 위하여 각 검사 년도간에 반복 측정된 분산분석을 시행하였다. 각 혈청지질의 지속성을 보기 위해서는 첫께, 초기값을 바탕으로 사분위 집단으로 나누어 각 사분위 집단의 혈청지질치가 매 검사 연도마다 어떻게 변하는 지를 그림으로 나타내었다. 둘째, 혈청지질치의 검사 시기사이의 스피어맨 상관계수를 구하였다. 그리고 마지막으로 각 검사 년도의 혈청지질치를 사분위 집단으로 구분하여 초기검사에서 가장 위험도가 큰 집단에 속한 사람들이 지속적으로 위험도가 큰 집단에 속하는 백분율을 계산하였다. 연구결과 남녀 모두 4년간 세 번의 검사에서 혈청 총 콜레스테롤은 남자가 초기검사에서 $160.7{\pm}25.3mg/dl$, 최종 추적검사에서 $150.0{\pm}27.8mg/dl$, 여자가 초기검사에서 $168.8{\pm}29.7mg/dl$, 최종 추적검사에서 $168.8{\pm}29.7mg/dl$, 중성지방은 남자가 $98.0{\pm}45.7mg/dl$에서, $114.1{\pm}67.9/mg/dl$로, 여자가 $109.9\geq37.1mg/dl$에서, $104.9{\pm}47.6mg/dl$로, LDL 콜레스테롤은 남자가 $93.6{\pm}21.2mg/dl$에서, $83.0{\pm}25.0mg/dl$로 여자가 $83.0{\pm}25.0mg/dl$에서, $94.7{\pm}24.4mg/dl$로, HDL 콜레스테롤은 남자가 $47.6{\pm}9.1mg/dl$에서, $44.2{\pm}9.4mg/dl$로, 여자가 $48.8{\pm}10.0mg/dl$에서, $47.8{\pm}11.3mg/dl$ 변화하였다. 반복 측정한 분산분석 결과 남녀 모두 4년 동안 혈청 총 콜레스테롤, LDL 콜레스테롤, HDL콜레스테롤이 통계적으로 유의하게 감소하였다. 특히 남자 LDL 콜레스테롤은 두 번의 추적검사에서 모두 감소한 것으로 나타났다. 중성지방은 남자에서는 지속적으로 증가하였고 여자에서는 변화가 없었다. 같은 연령에서 남녀간의 각 혈청지질치를 비교해보면 중성지방을 제외하고는 모두 여자가 남자보다 더 컸다. 혈청 지질의 초기 검사와 최종 검사간의 스피어맨 상관분석과 각 혈청 지질치의 위험도가 가장 큰 사분위집단에 잔류하는 백분율을 보면 지속성이 존재하였다. 혈청 총 콜레스테롤과 HDL 콜레스테롤에서 각 시기의 검사치들의 상관계수는 남자가 0.6 이상, 여자가 약 0.7 이상이었으며 통계적으로 유의하였다. 첫 번께 검사에서 위험도가 가장 큰 사분위 집단에 속한 사람 중 두 번째 검사에서도 위험도가 가장 큰 사분위 집단에 속한 백분율은 여자의 중성지방(42.9%)을 제외하고는 모두 50%이상이었다. 이들이 세 번째 검사에서도 같은 사분위 집단에 잔류한 백분율은 It has been known that there is a tracking phenomenon in the level of serum lipids. However, no study has been performed to examine the change and tracking of serum lipids in Korean adolescents. The purpose of this study is to examine the changes of serum lipids in Korean adolescents from 12 to 16 years of age, and to examine whether or not there is a tracking phenomenon in serum lipids level during the period. In 1992 serum lipids(total cholesterol(TC), triglyceride(TG), LDL cholesterol(LDL-C), HDL cholesterol(HDL-C)) were measured in 318 males, 365 females who were 12 years of age in Kangwha county, Korea. These participants have been followed up to 1996 and serum lipids level were examined in 1994 and 1996. Among the participants 162 males and 147 females completed all three examinations in fasting state. To examine the effect of eliminating adolescents with incomplete data, we compared serum lipids, blood pressure and anthropometric measures at baseline between adolescents with complete follow-up and adolescents who were withdrawn. To examine the change of serum lipids we compared mean values of serum lipids according to age in males and females. Repeated analysis of variance was used to test the change according to age. We used three methods to examine the existence of tracking. First, we analyzed the trends in serum lipids over 4-year period within quartile groups formed on the basis of the first-year serum lipids level to see whether or not the relative ranking of the mean serum lipids among the quartile groups remained in the same group for 4-year period. Second, we quantified the degree of tracking by calculating Spearman's rank correlation coefficient between every tests. Third, the persistence extreme quartile method was used. This method divides the population into quartile groups according to the initial level of blood lipids and then calculates the percent of the subjects who stayed in the same group at follow-up measurement. The decreases in levels were noted during 4 years for TC, LDL-C, primarily for boys. The level of HDL-C decreased between baseline and first follow-up for both sexes. Tracking, as measured by both correlation coefficients and persistence extreme quartiles, was evident for all of the lipids. The correlation coefficients of TC between baseline and 4 years later in boys and girls were 0.55 and 0.68, respectively. And the corresponding values for HDL-C were 0.58 and 0.69. More than 50% of adolescents who belonged to the highest quartile group in TC, HDL-C and LDL-C at the baseline were remained at the same group at the examination performed 2 years later for both sexes. The probabilities of remaining at the same group were more than 35% when examined 4 years later. The tracking phenomenon of TG was less evident compared with the other lipids. Percents of girls who stayed at the same group 2 years later and 4 years later were 42.9% and 25.7%, respectively. It was evident that serum lipid levels tracked in Korean adolescents. Researches with longer follow-up would be needed in the future to investigate the long-term change of lipids from adolescents to adults.

공동주택의 관리비 추정모델 연구

이강희,양재혁,채창우,Lee, Kang-Hee,Yang, Jae-Hyuk,Chae, Chang-U 한국주거학회 2010 한국주거학회 논문집 Vol.21 No.2

The maintenance cost plays a important role to plan the scale of the apartment housing such as a number of household, building area and building type. Therefore, it is required to forecast the cost considering various maintenance characteristics. The maintenance characteristics are floor area, number of household, heating type, site area and etc.. In addition, the maintenance cost are classified into 5 area. These are a personal expense, facility maintenance cost, energy and water cost, insurance and sanitary cost. These five cost area are related with various characteristics and brought up the estimation model using the stepwise multiple regression analysis. The energy and heating cost share over the 50% in the total cost and the personal expense cost shares about 40%. The personal expense cost per area is 5,272 won/$m^2{\cdot}yr$ irregardless of heating type and the district heating type is a higher cost than other type. In facility maintenance cost, the central heating type is 2,015 won/$m^2{\cdot}yr$ and higher than other type. The estimation models have good statistics in each model. Most of the model have a determination coefficient over 0.7 and Durbin Watson value between 1.5 and 2.5.

임대공동주택 구성재의 열화도 패턴에 관한 연구

이강희,Lee Kang-Hee 한국주거학회 2006 한국주거학회 논문집 Vol.17 No.4

Most of buildings have been deteriorated with time-elapse by reflection of the building location, material, environmental circumstances and so on. The performance would go down and be demolished if anything could not be done after constructed. The maintenance should be required to preserve a decent living condition or improve a inferior condition by various plans and practices. The maintenance plan needs various data such as a repair scope, a repair time, a forecasted cost, a plan of management and so forth. Among the above required data for planning the maintenance, the deterioration characteristics of the building components would be first analyzed. The deterioration pattern would be a key role to affect and make a maintenance plan. In this paper, it aimed at classifying the deterioration patterns of building components. A deterioration pattern would be analyzed between the cumulative repair cost and time-elapse and modeled with these relations. A deterioration patterns are classified into 4 types-a accelerated type, a straight type, a temporary type and a slowly type. As a result of this research, a accelerated type includes window, window frame, general paintings, general water proofing in building components. A straight type includes the lacquer paintings, furnishings in building components and water supply pipe, boiler, sanitaries in mechanical facilities and lighting in electric facilities. Based on these research results, further study should be conducted to include any other components and an estimating model.