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한의학 고문헌의 단방용례 분석을 통한 천문동의 기대효능 연구
최고야(Choi Goya),윤태숙(Yoon Taesook),추병길(Choo Byungkil),문병철(Moon Byeongcheol),채성욱(Chae Sungwook),김호경(Kim Hokyoung) 한국한의학연구원 2008 한국한의학연구원논문집 Vol.14 No.1
We referred to twenty-two Korean medicinal literatures for application of a single-medicine prescription using Asparagi Tuber(Asparagus cochinchinensis) to form the groundwork for scientific modernization of Korean medicine. Our study revealed the following. 1. Principle expectant effects of Asparagi Tuber were prolong life; elevation of stamina or activity; improvement of asthenia; remedy of epilepsy, mental disease or convulsions; relief of xerosis; treatment of tumor, abscess or intumescence; extermination of endoparasite; solution of numbness, etc. 2. The records show that 95% of directions are per oral. 3. 38% of the cases, Asparagi Tuber was used without its jacket or core. 4. Alcohol and honey were generally used as solvent and additive. We suggest to perform the further studies for the scientific verification of the expectant effects of Asparagi Tuber and its different efficacy by processing, solvent and additives.
한의학 고문헌 분석을 통한 무궁화의 약용부위와 기대효능 연구
최고야(Choi Goya),윤태숙(Yoon Taesook),추병길(Choo Byungkil),이아영(Lee Ayeong),채성욱(Chae Sunguk),주영승(Ju Youngsung),김호경(Kim Hokyoung) 한국한의학연구원 2007 한국한의학연구원논문집 Vol.13 No.2
Hibiscus syriacus is rarely used herb. For finding up new applications of this herb, the medicinal parts and expected efficacys that recorded in 24 classics of oriental medicine were inquired. As a result of the literature review, medicinal parts and expected efficacys of Hibiscus syriacus were as follows: The medicinal parts were mainly flower and bark But the root, branch, leaf and fruit were mentioned too. Some unidentified medicinal parts seemed to be branch or bark. The mentioned symptoms that prescribed Hibiscus syriacus are intestinal 'wind', red-white dysentery, thirst with fever after dysentery, insomnia, 'wind' syndrom, leukorrhea, regurgitation, etc. And this herb was mentioned that applied externally for scabies, hemorrhoids, prolapse of the rectum, headache, extracted tooth, etc. Therefore, bleeding intestinal diseases, insomnia, vaginal or uterine hemorrhage(for internal use), scabies or mycosis, hemorrhoids, anal fistula, prolapse of the rectum, headache(for external use) were presented as expected efficacys of Hibiscus syriacus. In future, studies for verifying the efficacys are needed.
멸종위기 희귀식물인 갯방풍 자생지별 유전변이 및 유전적 다양성 연구
문병철(Moon Byeongcheol),추병길(Choo Byungkil),지윤의(Ji Yunui),윤태숙(Yoon Taesook),김호경(Kim Hokyoung) 한국한의학연구원 2008 한국한의학연구원논문집 Vol.14 No.3
Glehnis littoralis Fr. Schmidt et Miquel is an important medicinal plants in East Asian countries. This plant species naturally distributed in Korea, Japan, China. and Taiwan, but it is a rare plants living in the coastal dune in Korea. To investigate the genetic variation, genetic diversity and genetic evolutionary relationships of 14 different geographical G. littorslis, ITS sequence and random amplified polymorphic DNA (RAPD) were analyzed. On the basis of ITS sequences, it was clearly showed that the ITS1 and ITS2 sequences among 14 populations are identical regardless of geographical origin excepting 2 bp in pair-wise comparison of ITS1. Furthermore, RAPD results also showed that 14 different geographical G. littoralis produce various polymorphic patterns without critical relationship among neighboring regions. These combined results suggest that the geographical variation and genetic evolution of G. littoralis is stable and provide important information on genetic diversity, and conservation of this rare plant species in situ and ex situ.
ITS 부위 염기서열과 RAPD분석을 통한 천문동의 지역별 변이 및 분자진화적 유연관계
문병철(Moon Byeongcheol),추병길(Choo Byungkil),지윤의(Ji Yunui),최고야(Choi Goya),윤태숙(Yoon Taesook),이아영(Lee Ayoung),김호경(Kim Hokyoung) 한국한의학연구원 2008 한국한의학연구원논문집 Vol.14 No.1
Phylogenetic relationship and DNA polymorphism among local populations of the Asparagus cochinchinensis have been investigated based on nuclear ribosomal DNA ITS sequences and RAPD analysis in Korea. In result, two genetically distinct groups of local populations except Geoje were recognized by the phylogenetic tree both in rDNA-ITS and RAPD. One was called ‘western coast group’ that includes the Buan 1, 2 and Taean and the other was ‘southern coast group’ that includes Haenam, Yeosu and Namhae. Thus, the geographical relationship of Asparagus cochinchinensis was two well-typified clades. These results suggest that the geographical genetic variation of Asparagus cochinchinensis is closely connected with the slow and long period of propagation via the coast in Korean Peninsula.
모시대(Adenophora remotiflora) 개체군의 식생구조에 관한 연구
추병길(Choo Byungkil),지윤의(Ji Yunui),문병철(Moon Byeongcheol),윤태숙(Yoon Taesook),채성욱(Chae Sungwook),김호경(Kim Hokyoung) 한국한의학연구원 2008 한국한의학연구원논문집 Vol.14 No.1
Objectives : This study was carried out to investigate vegetation structure of Adenophora remotiflora population found to be distributed in Jeombongsan Danmokryeong. Methods : From 2007 June until November, 2 m × 2 m quadrat was established in Adenophora remotiflora community in order to record a dominants and coverage. Results: 1. The vegetation of Adenophora remotiflora community was classified into Astilbe chinensis subcommunity and Calamagrostis langsdorffii subcommunity. Differential species of community was Angelica deacrusica and Lychnis cognata. The Adenophora remotiflora community was found in south face, high of altitude and low slope degree. 2. The value of species diversity ranged from 5.547152 to 10.077886, euenness ranged from 0.779749 to 0.986358 and dominance ranged from 0.013642 to 0.220251. 3. Aconitum jaluense was located at the higher altitudes than those of other species. Lychnis cognata, Lychnis cognata and Pedicularis resupinata was located at the lower altitudes and slope degree than those of other species. Conclusions : The vegetation of Adenophora remotiflora community was classified into Astilbe chinensis subcommunity and Calamagrostis langsdorffii subcommunity. The value of species diversity was low and community was dominated by a large number species. The Adenophora remotiflora community was found in south face, high of altitude and low slope degree.
RAPD 분석을 통한 대황(大黃)과 종대황(種大黃) 감별용 SCAR 유전자 마커 개발
문병철 ( Byeong Cheol Moon ),이영미 ( Young Mi Lee ),천진미 ( Jin Mi Chun ),이아영 ( A Young Lee ),윤태숙 ( Taesook Yoon ),전명숙 ( Myeong Sook Cheon ),추병길 ( Byung Kil Choo ),김호경 ( Ho Kyoung Kim ) 대한본초학회 2009 大韓本草學會誌 Vol.24 No.4
Objectives: Due to the morphological similarity and frequent occurrence of intermediate forms as well as morphological variations of aerial part, the correct identification between Rhei Radix et Rhizoma and Rhei Undulatai Rhizoma is very difficult. To develop a reliable method for correct identification and improving the quality standards of Rhei Radix et Rhizoma and Rhei Undulatai Rhizoma, we analyzed RAPD and developed SCAR marker. Methods: To amplify target DNA at the genomic level, 32 Operon 10-mer random primers were applied with four Rheum species, R. officinale, R. palmatum, R. tanguticum and R. undulatum. The nucleotide sequences were determined and species-specific primers were prepared depending on the species-specific RAPD amplicons after subcloned into the pGEM-Teasy vector. To develop the SCAR markers, species-specific PCR amplification and mutiplex-PCR were carried out using the single species-specific primer pairs and combinations of them, respectively. Results: We used RAPD analysis of four Rheum plant species to obtain several species-specific RAPD amplicons. From nucleotide sequences of these RAPD amplicons, we developed two SCAR markers that amplified 314 bp and 390 bp DNA fragments in only R. undulatum but not in R. officinale, R. palmatum, R. tanguticum and R. undulatum, for distinguishing Rhei Undulatai Rhizoma and Rhei Radix et Rhizoma. Furthermore, we established SCAR markers for the simultaneous discrimination of the three species within a single reaction by using multiplex-PCR. Conclusions: These genetic markers can be used for the efficient discrimination of plants species and commercial herbal medicines between Rhei Undulatai Rhizoma and Rhei Radix et Rhizoma, to ultimately prevent indiscriminate distribution and prescription of these herbal medicines.