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멸종위기 희귀식물인 갯방풍 자생지별 유전변이 및 유전적 다양성 연구
문병철(Moon Byeongcheol),추병길(Choo Byungkil),지윤의(Ji Yunui),윤태숙(Yoon Taesook),김호경(Kim Hokyoung) 한국한의학연구원 2008 한국한의학연구원논문집 Vol.14 No.3
Glehnis littoralis Fr. Schmidt et Miquel is an important medicinal plants in East Asian countries. This plant species naturally distributed in Korea, Japan, China. and Taiwan, but it is a rare plants living in the coastal dune in Korea. To investigate the genetic variation, genetic diversity and genetic evolutionary relationships of 14 different geographical G. littorslis, ITS sequence and random amplified polymorphic DNA (RAPD) were analyzed. On the basis of ITS sequences, it was clearly showed that the ITS1 and ITS2 sequences among 14 populations are identical regardless of geographical origin excepting 2 bp in pair-wise comparison of ITS1. Furthermore, RAPD results also showed that 14 different geographical G. littoralis produce various polymorphic patterns without critical relationship among neighboring regions. These combined results suggest that the geographical variation and genetic evolution of G. littoralis is stable and provide important information on genetic diversity, and conservation of this rare plant species in situ and ex situ.
모시대(Adenophora remotiflora) 개체군의 식생구조에 관한 연구
추병길(Choo Byungkil),지윤의(Ji Yunui),문병철(Moon Byeongcheol),윤태숙(Yoon Taesook),채성욱(Chae Sungwook),김호경(Kim Hokyoung) 한국한의학연구원 2008 한국한의학연구원논문집 Vol.14 No.1
Objectives : This study was carried out to investigate vegetation structure of Adenophora remotiflora population found to be distributed in Jeombongsan Danmokryeong. Methods : From 2007 June until November, 2 m × 2 m quadrat was established in Adenophora remotiflora community in order to record a dominants and coverage. Results: 1. The vegetation of Adenophora remotiflora community was classified into Astilbe chinensis subcommunity and Calamagrostis langsdorffii subcommunity. Differential species of community was Angelica deacrusica and Lychnis cognata. The Adenophora remotiflora community was found in south face, high of altitude and low slope degree. 2. The value of species diversity ranged from 5.547152 to 10.077886, euenness ranged from 0.779749 to 0.986358 and dominance ranged from 0.013642 to 0.220251. 3. Aconitum jaluense was located at the higher altitudes than those of other species. Lychnis cognata, Lychnis cognata and Pedicularis resupinata was located at the lower altitudes and slope degree than those of other species. Conclusions : The vegetation of Adenophora remotiflora community was classified into Astilbe chinensis subcommunity and Calamagrostis langsdorffii subcommunity. The value of species diversity was low and community was dominated by a large number species. The Adenophora remotiflora community was found in south face, high of altitude and low slope degree.
한의학 고문헌의 단방용례 분석을 통한 천문동의 기대효능 연구
최고야(Choi Goya),윤태숙(Yoon Taesook),추병길(Choo Byungkil),문병철(Moon Byeongcheol),채성욱(Chae Sungwook),김호경(Kim Hokyoung) 한국한의학연구원 2008 한국한의학연구원논문집 Vol.14 No.1
We referred to twenty-two Korean medicinal literatures for application of a single-medicine prescription using Asparagi Tuber(Asparagus cochinchinensis) to form the groundwork for scientific modernization of Korean medicine. Our study revealed the following. 1. Principle expectant effects of Asparagi Tuber were prolong life; elevation of stamina or activity; improvement of asthenia; remedy of epilepsy, mental disease or convulsions; relief of xerosis; treatment of tumor, abscess or intumescence; extermination of endoparasite; solution of numbness, etc. 2. The records show that 95% of directions are per oral. 3. 38% of the cases, Asparagi Tuber was used without its jacket or core. 4. Alcohol and honey were generally used as solvent and additive. We suggest to perform the further studies for the scientific verification of the expectant effects of Asparagi Tuber and its different efficacy by processing, solvent and additives.
ITS 부위 염기서열과 RAPD분석을 통한 천문동의 지역별 변이 및 분자진화적 유연관계
문병철(Moon Byeongcheol),추병길(Choo Byungkil),지윤의(Ji Yunui),최고야(Choi Goya),윤태숙(Yoon Taesook),이아영(Lee Ayoung),김호경(Kim Hokyoung) 한국한의학연구원 2008 한국한의학연구원논문집 Vol.14 No.1
Phylogenetic relationship and DNA polymorphism among local populations of the Asparagus cochinchinensis have been investigated based on nuclear ribosomal DNA ITS sequences and RAPD analysis in Korea. In result, two genetically distinct groups of local populations except Geoje were recognized by the phylogenetic tree both in rDNA-ITS and RAPD. One was called ‘western coast group’ that includes the Buan 1, 2 and Taean and the other was ‘southern coast group’ that includes Haenam, Yeosu and Namhae. Thus, the geographical relationship of Asparagus cochinchinensis was two well-typified clades. These results suggest that the geographical genetic variation of Asparagus cochinchinensis is closely connected with the slow and long period of propagation via the coast in Korean Peninsula.
갯방풍의 기내부정아 형성에 미치는 식물생정조절물질의 영향
추병길(Choo Byungkil),지윤의(Ji Yunui),문병철(Moon Byeongcheol),최고야(Choi Goya),이혜원(Lee Hyewon),이아영(Lee Ayeong),김호경(Kim Hokyoung) 한국한의학연구원 2008 한국한의학연구원논문집 Vol.14 No.1
Petiole explant of Glehnia littoralis Fr. Schmidt was in vitro cultured MS plant medium(DUCHEFA co.) supplemented with various plant growth regulators and examined to find out their optimum combination and concentration for plantlet regeneration. We investigated optimal condition for efficient plant regeneration through adventitious shoot formation on MS plant medium with various kinds of plant growth regulators. Embryogenic calli and adventitious shoot formation were greatly influenced by plant growth regulators. Embryogenic calli induction showed a good response on MS medium supplemented with NAA and BA than others. Especially, combination of 1.0 ㎎/L NAA and 0.5 ㎎/L BA on MS medium led to the greatest frequency in adventitious shoot. The results suggest that plant regulator selection be important factor to achieve an efficient regeneration.
최고야(Choi Goya),추병길(Choo Byungkil),문병철(Moon Byeongcheol),이혜원(Lee Hyewon),이아영(Lee Ayeong),김호경(Kim Hokyoung) 한국한의학연구원 2007 한국한의학연구원논문집 Vol.13 No.3
Haedae(海帶, haidai) is representative herb from seaweeds. but its origin is still confusing. Thus we analyzed its origin on the basis of classics of oriental medicine. The scientific name of Haedae corresponds to Zostera marina, according to the folkloric use. But, it is Laminaria japonica. according to its habitat, morphological feature, and the korean name on the medical books in Joseon since 17c. In the remote past, it seemed that two groups were segragated by the name of Zostera marina Haedae and Laminaria japonica Haedae. However, it is thought that this two trends were mixed up when medical knowledge was compiled in Song Dynasty era. Documental support inclined to Laminaria japonica, but it is too early to conclude that Laminaria japonica is the Haedae. To prove this hypothesis, further study is required to investige whether Laminaria japonica or Zostera marina has mentioned efficacies of Haedae used for the treatment of wen. San syndromes, edema. scrofula, etc. And we have need of research that solve the origin problem of Gonpo(Laminariae thallus) on the assumption that Laminaria japonica is Haedae.
이혜원(Lee Hyewon),박소영(Park Soyoung),이아영(Lee Ayoung),채성욱(Chae Sungwook),최고야(Choi Goya),추병길(Choo Byungkil),김호경(Kim Hokyoung) 한국한의학연구원 2008 한국한의학연구원논문집 Vol.14 No.1
Objectives : Cynanchum wilfordii(Asclepiadaceae) has been traditionally used as a tonic, the prevention and treatment of various geriatric diseases in Korea. Acetophenone derivatives from C. wilfordii showed neuroprotective activity. In this study, two acetophenones were isolated and quantitative determination of acetophenones from C. wilfordii has been developed for quality stand. Methods : Three acetophenone derivatives were isolated from methanol extract of C. wilfordii by the chromatographic separation. Their structures were identified as cynandione A, 2,5-dihydroxy acetophenone and cynanchone A on the basis of spectral data(MS, ¹H-NMR, ¹³C-NMR) and chemical analysis. HPLC analysis was performed to determine the contents of cynandione A and 2,5-dihydroxyacetophenone in C. wilfordii. Results: According to the results, the contents of cynandione A and 2,5- dihydroxyacetophenone were 0.274%, 0.035% by HPLC, respectively. Conclusions: In these results, we have determined the contents of cynandione A and 2.5-dihydroxyacetophenone in Cynanchum wilfordii, respectively. We hope that this study will contribute to the standardization and quality control of herbal medicine.
한의학 고문헌 분석을 통한 무궁화의 약용부위와 기대효능 연구
최고야(Choi Goya),윤태숙(Yoon Taesook),추병길(Choo Byungkil),이아영(Lee Ayeong),채성욱(Chae Sunguk),주영승(Ju Youngsung),김호경(Kim Hokyoung) 한국한의학연구원 2007 한국한의학연구원논문집 Vol.13 No.2
Hibiscus syriacus is rarely used herb. For finding up new applications of this herb, the medicinal parts and expected efficacys that recorded in 24 classics of oriental medicine were inquired. As a result of the literature review, medicinal parts and expected efficacys of Hibiscus syriacus were as follows: The medicinal parts were mainly flower and bark But the root, branch, leaf and fruit were mentioned too. Some unidentified medicinal parts seemed to be branch or bark. The mentioned symptoms that prescribed Hibiscus syriacus are intestinal 'wind', red-white dysentery, thirst with fever after dysentery, insomnia, 'wind' syndrom, leukorrhea, regurgitation, etc. And this herb was mentioned that applied externally for scabies, hemorrhoids, prolapse of the rectum, headache, extracted tooth, etc. Therefore, bleeding intestinal diseases, insomnia, vaginal or uterine hemorrhage(for internal use), scabies or mycosis, hemorrhoids, anal fistula, prolapse of the rectum, headache(for external use) were presented as expected efficacys of Hibiscus syriacus. In future, studies for verifying the efficacys are needed.
천진미(Chun Jinmi),장설(Jang Seol),심지훈(Shim Jihoon),이아영(Lee Ayeong),전원경(Jeon Wonkyung),이혜원(Lee Hyewon),추병길(Choo Byungkil),김호경(Kim Hokyoung) 한국한의학연구원 2006 한국한의학연구원논문집 Vol.12 No.3
This study was investigated to determine the quality monitoring of distributed materials from Glycyrrhizae Radix (26 samples), Atractylodis Rhizoma Alba (24 samples) according to storage period after 1-3 year. We have estimated by identification, purity, loss on drying, ash, acid insoluble ash, extract content, essential oil content, assay and microbial contamination, As a result, Glycyrrhizae Radix (26 samples) were satisfied with the standard of KP. (Korean Pharmacopoeia) and WHO's microbial contamination limit standard. In the Atractylodis Rhizoma Alba (24 samples), 2 samples were not satisfied with the standard of KP.(Korean Pharmacopoeia) and WHO's microbial contamination limit standard. The results make practical application of the basic data for the quality control of herbal medicine in storage.
고병섭,이미영,김호경,전원경,정계진,주영승 한국한의학연구원 2000 한국한의학연구원논문집 Vol.6 No.1
The purpose of this study is to encourage the circulation of good quality of oriental medicine and to improve national welfare. Investigation for the induction of quality inscription system was conducted by mail and interview. The data were analyzed by SAS. The major results were as follows; The portion of subjects agree to necessity of quality inscription was 78.5%. In the question of side effect against quality inscription, the rate of negative was 67.7%. The responses answered that the necessity of quality inscription was not economical profit but authority of knowledge and improvement on health(37.8%), established market for herbal medicine(34.7%) and effect of clinical care(23.9%), respectively. These results might be useful information for establishing of quality inscription system for herbal medicine.