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볏짚 - 계분 발효사료 급여시 비유효과에 미치는 영향 및 발효사료의 안전성에 관한 연구
윤칠석,박전홍,오태광,이남형,김춘수,지설하,오대균,김준식 ( C . S . Yoon,J . H . Park,T . K . Oh,N . H . Lee,Chu S . Kim,S . H . Chee,D . K . Ohh,J . S . Kim ) 한국축산학회 1985 한국축산학회지 Vol.27 No.8
This experiment was carried out to evaluate the effects of feeding straw-manure silage compared with corn silage, and were consisted of trial I by Holstein cows and trial II by sheep with change-over feeding. And trial II was used to evaluate the digestibility and ruminal characteristics. The ratio of formula feed to each silage during experimental period was maintained at 40: 60 (w/w) as DM basis. Data from trial I on corn and straw-manure silage group, respectively, were; daily milk yield 17.23, 17.05㎏; milk fat 4.06, 4.19%; milk protein 3.89, 3.92%; milk lactose 5.27, 5.17%; SNF 10.36, 10.38%. These values were not significantly different between treatments. The digestibility of ration components on trial II showed that the digestibility of proximate composition of corn silage group tended to be slightly increased. But the fibrous matetial digestibility of straw-manure silage showed more increased results (p$lt;.05). Ruminal parameters on pH and VFA concentration were similar with two silage group. However, straw-manure silage resulted in stepwise increase in propionate and valerate throughout the sampling time. Blood parameters and parasites were observed to elucidate the possible harzardness of straw-manure silage feeding in dairy cattle. No difference was observed in PCV, Hb, plasma prote in, and BUN. Cholesterol and ALT were appeared slightly high value in straw-manure silage feeding group, however, plasma glucose was neverse. Nematodes survived up to 40 fermentaton days, and mites up to 30 days. Protozoa died within 10 fermentation days.
In Situ 방법에 의한 국내이용 단백질 사료원에 대한 단백질 분해율에 관한 조사
윤칠석(C . S . Yoon),김덕영(D . Y . Kim),이남형(N . H . Lee) 한국축산학회 1990 한국축산학회지 Vol.32 No.5
The protein degradability of common feedstuffs utilized in Korea was evaluated in situ. The protein degradability was determined in nylon bags suspended in the rumen of sheep for 2, 5, 8, 12, 24, 48 and 72h. The used nylon bags have 625um² of pore size and l2x7cm of bag size. And experimental diets were consisted of 900g concentrates, 400g 3cm-chopped rice straw and 400g alfalfa pellet, and this diet was fed twice a day. The protein degradability of cottonseed oil meal, corn germ meal and coconut oil meal was greatly influenced when the rate of rumen turnover was changed from 0.01/h to 0.10/h. The degradabilities of fish meal, corn gluten meal and meat and bone meal were low compared with other protein feedstuffs. Especially, the degradability of perilla oil meal was similar with fish meal and corn gluten meal and it was evaluated as protein source with low degradability of protein.
Nylong Bag Test 에서 제1위 미생물에 의한 오염도 및 오염된 미생물 단백질을 보정한 사료단백질 분해율의 측정
윤칠석(C . S . Yoon),김덕영(D . Y . Kim),이남형(N . H . Lee) 한국축산학회 1991 한국축산학회지 Vol.33 No.1
This experiment was conducted to evaluate the extent of rumen microbial contamination of feed residues in the nylong bag test and estimates of microbially corrected protein degradability were made with diaminopomelic acid (DAPA) content of values for microbial protein contribution. Nylon bag (a pore size of 625u㎡, 6×12cm internal dimensions) containing 4.0-4.5g sample were incubated in the rumen for 5, d, 12 and 24h. The bacterial marker for the calculation of the proportion of microbial protein in the residues after rumen incubation was DAPA. The calculation method for microbially corrected values was presented as 1) DAPA correction of residues and 2) DAPA correction of feed and residues. The content of DAPA in residues increased with incubation time. And the proportion of residual N as microbial origin also increased linearly with time in the rumen for soybean meal and cottonseed meal, but this tended to be decreased with increased incubation time for fish meal andcorn gluten meal. The mean difference of N degradability between uncorrected and corrected values for microbial contamination in the residue was 16% at plant protein sources (soybean meal, cottonseed meal, and corn gluten meal) and this was 9.2% at animal protein sources (fish meal, and. meat and bone meal). This mean difference for amino acids degradability was 20.6% at plant protein sources and 7.7% at animal protein sources, respectively. And the corrected degradability by DAPA content in the residue for N, nonessential and essential amino acids was simular with the calculation method of DAPA correction of feed and residues at soybean meal, corn gluten meal and meat ant bone meal.
회장에 (回腸) Cannula 를 설치한 돼지에서 아미노산의 생체내 이용성
박병성(B . S . Park),윤칠석(C . S . Yoon),이남형(N . H . Lee),지규만(K . M . Chee) 한국축산학회 1989 한국축산학회지 Vol.31 No.10
Bioavailability of dietary amino acids were measured in swine fitted with ileal T-cannula. Three swine with average body weight 50± 1.7kg were fed diets containing soybean meal or fish meal as the only N sources. After 5 days of preliminary feeding period, ileal digesta and anal excreta were collected for two consecutive days. N-free diet composed of glucose, corn oil, α-cellulose and vitamin-mineral premix were also fed to evlauate amount of endogenous amino acids. Amount of amino acids disappeared were generally larger in fecal excreta than in ileal digesta with a significant difference(p $lt;0.05) for fish meal. Apparent availabilities of amino acids in soybean meal and fish meal were 86.7 and 83.4% for ileal digesta, and 91.7 and 91.1% for feces, respectively. True availabilities of amino acids in soybean meal and fish meal were 89.5 and 86.5% for ileal diegesta, and 92.7 and 92.2% for fecal excreta, respectively. Amount of amino acids in soybean meal appeared better utilized than those in fish meal evaluated in ileal digesta. No such trend, however, was observed in fecal excreta. It appeared that in vivo bioavailability of dietary amino acids in swine should be studied with ileal digesta rather than fecal material.