數種防腐劑의 殺菌效果에 關한 實驗的 硏究

尹壽漢(Soo Han Yoon) 대한치과의사협회 1977 대한치과의사협회지 Vol.15 No.12

生活齒髓覆조術後의 治癒 結果에 對한 臨床的考察

尹壽漢(Soo Han Yoon),孫同銖(Dong Su Shon) 대한치과의사협회 1972 대한치과의사협회지 Vol.10 No.12

수아세포종 세포와 교모세포종 세포의 배양에서 all-trans와 13-cis retinoic acid의 세포증식억제 효과

윤수한(Soo Han Yoon),김세혁(Se Hyuk Kim),안영환(Young Hwan Ahn),안영민(Young Min Ahn),조기홍(Ki Hong Cho),조경기(Kyung Gi Cho),김성환(Sung Hwan Kim) 대한소아신경학회 1998 대한소아신경학회지 Vol.5 No.2

목적 : 암화학요법중에 대표적인 약물중의 하나인 레티노익 산을 이용하여 신경모세포종과 교모세포종을 포함하여 여러 암세포에서 증식억제를 이룰 수 있다고 하지만, 최근에 악성 교종 세포종에서의 임상적 시도에서 약물반응도가 낮았다는 상반된 결과를 해결하기 위해 혈중 유효약물농도에서 종양증식억제 정도를 측정해보고자 하였다. 또한, 레티노익 산의 작용이 종양세포의 분화를 촉진시키고 종양의 증식을 억제하는 것이라면 당연히 성인종양보다는 소아종양에서 효과가 기대되는 만큼 뇌종양에서는 무엇 보다도 대표적인 소아종양인 수아세포종에서 효과를 확인해보고자 하였다. 대상및 방법 : 수아세포종 환자의 생검조직에서 배양된 수아세포종과 교모세포종 373과 87세 포주를 배양하여 all-trans 와 13-cis 레티노익 산의 농도를 10¯5M에서 10¯¹¹M까지 10¯5M, 10¯6, 10¯7, 10¯8, 10¯9, 10¯10 및 10¯¹¹M등의 7단계로 나누고 4일, 7일 그리고 14일간 배양후 각 단계별 세포성장억제(cell growth inhibition) 정도를 MTT 검사에 의해 비교하였다. 결과 : 87세포주에서 13-cis 레티노익 산의 경우에 10¯8M부터 10¯7M까지에서는 10~15%의 종양증식억제가 이루어졌지만 10¯6M에서 10¯5M의 농도에서는 증식억제가 4일에는 10-22%, 7일에는 10%, 14일에는 0~12%였고, all trans 레티노익산의 경우에는 10¯6M 이하 농도에서는 4일, 7일, 그리고 14일 모두에서 5% 미만의 증식억제를 보였는데 10¯5M 농도에서는 4일과 7일에는 증식억제가 42%와 37%였지만, 14일에는 0%로서 시간 경과에 따라 증식억제가 감소하였다. 373 세포주에서는 10¯6M과 10¯5M 농도의 all-trans 레티노익 산에 의해서 10¯6M과 10¯5M 농도에서 4일과 7일에는 5% 이하의 증식억제를 보이다가 14일에는 10-15%의 증식억제를 보였으며 13-cis 레티노익 산의 경우에는 10¯6M과 10¯5M에서 4일에는 약 30%, 7일에는 약 20%, 그리고 14일에도 약 20%의 증식억제를 보였다. 수아세포종에서는 10¯6M 이하 농도의 all-trans와 13-cis 레티노익 산에 의해서 증식억제가 약 25% 이상 이루어졌으며, 10¯6M 이상 농도에서 13-cis 레티노익 산의 경우 25% 정도의 증식억제를 보였지만 all-trans 레티노익 산에서는 약 40%의 증식억제를 볼 수 있었다. 결론 : 교모세포종의 경우 인체에 투여가능한 정도의 all-trans 또는 13-cis 레티노익 산 농도에서 각 세포주의 반응도가 달라서 그 효과를 예측할 수 없지만, 수아세포종에서는 13-cis 와 all-trans 레티노익 산 모두에서 종양 증식억제 효과가 있었으며, 특히 all-trans 이형체가 효과가 더 좋았으며, 또한 지속적 투여와 저농도(10¯9M 이하)에서도 종양증식 억제에 효과가 있었다. propose : It has been reported that retinoic acid, one of the most popular agents for chemoprevention could inhibit the proliferation of many cancer cells including neuroblastoma and glioblastoma. However, there is increasing demand reaccessing its in vitro inhibitory errect on the tumor proliferation because of the poor results from recent clinical trials of retinoic acid in the malignant brain tumor. Retinoic acid promoted the diffferentiation and apoptosis of tumor cell so that its effect might be obvious in the pediatric brain tumor. Therefore we are going to confirm the effectiveness of retinoic acid to inhibit the proliferation of the tumor cells; glioblastoma and medulloblastoma in childhood. Methods : Medulloblastoma cells were derived from the primary culture of the patient's specimen, and glioblastoma cells were cell lines of 373-MG and 87-MG. We estimated growth inhibition rate of each tumor cells using MTT assay in th concentration from 10¯¹²M to 10¯5M of all-trans and 13-cis retinoic acid. Results : 13-cis retinoic acid in the concentration of 10¯6M inhibited cell growth rate 10-22% on the 4th day of incubation, 10% on the 7th day, and 0-12% on the 14th day in the concentration from 10¯6M to 10¯5M. All-trans retinoic acid inhibited cell growth rate less than 5% in the concentration less than 10¯5M though the whole incubation period, but 42% on the 4th day, 37% on the 7th day, and 0% on the 14th in the concentration of 10¯5M. 13-cis retinoic acid inhibited cell growth rate 30% on the 4th day, 20% on the 7th and 14th day in the concentration between 10¯6M and 10¯5M. All-trans retinoic acid inhibited cell growth rate less than 25% by all-trans and 13-cis retnoic acid in the concentration less than 10¯6M. 13-cis retinoic acid showed 25% growth inhibition in the concentration above 10¯6M, but all-trans retnoic acid showed 40% growht inhibition in the same concentration. Conclusion : We could not find the effect of retnoic acid in the glioblastoma cells due to variable responses of the tumor cell growht inhibition in the concentration of maximum tolerable dose. IIowever, there is a significant inhibition effect of medulloblastoma cell proliferation both in the 13-cis and all-trans retinoic acid.

Formaldehyed를 使用한 生活齒髓 切斷術後의 齒髓變化에 關한 實驗的 硏究

尹壽漢(Yoon Soo Han) 대한치과의사협회 1973 대한치과의사협회지 Vol.11 No.3

주파수의존 근관장 측정기 ROOT-ZX의 정확성에 관한 연구

박한수(Han-Soo Park),윤수한(Soo-Han Yoon) 대한치과의사협회 1997 대한치과의사협회지 Vol.35 No.5

GFP를 이용하여 in-vivo에서 추적한 Bad와 Bcl-XL의 Mitochondria 이동

윤수한,김진영,박승우,안영환,안영민,조기홍,조경기,Yoon, Soo Han,Kim, Jin Young,Park, Seung Woo,Ahn, Young Hwan,Ahn, Young Min,Cho, Ki Hong,Cho, Kyung Gi 대한신경외과학회 2000 Journal of Korean neurosurgical society Vol.29 No.10

Objectives : The subcellular locations of Bad, Bid, Bax and Bcl-XL change during apoptosis and this change is important for the regulation of cell death. The purpose this study was to elucidate binding of Bad with Bcl-XL in vivo Methods : We mads Bad with Green Fluorescent Protein(GFP) using PCR method. We transfected and overexpressed GFP-Bad with or without Bcl-XL cotransfection in living COS-7 cell. Results : Bad and Bcl- XL bind one another in healthy living cells and this association controled mitochondrial docking. In the absence of Bad-XL, Bad was mainly cytosolic and partially bound to mitochondria. Upon coexpression of Bad and Bcl-XL, most of Bad translocated to mitochondria. These should suggest that Bad binds to the mitochondrial and cytoplasmic forms of Bcl-XL and Bad bound to cytoplasmic Bcl-XL translocates to mitochondria. These in vivo findings confirm that Bad make a complexes with Bcl- XL and cause mitochondrial translocation of Bad-Bcl-XL complex.

온도변화(溫度變化)에 따른 치질(齒質)의 구조적변화(構造的變化)

윤수한,Yoon, Soo-Han 대한치과보존학회 1976 Restorative Dentistry & Endodontics Vol.2 No.1

16 healthy teeth extracted have been selected and their color and structural change have been observed at the fixed temperatures of $200^{\circ}F$., $400^{\circ}F$., $600^{\circ}F$., and $800^{\circ}F$. respectively at the intervals of 10min. 30min and 60min. The results were as follows: 1) $200^{\circ}F$ Groop: At the 60 minutes interval, crown surface shows pattern simliars to mottled teeth and roots take on light yellowish colar and interglobular dentin tends to be rough. 2) $400^{\circ}C$ Groop: at the 30 minutes interval, cracks begin to show in the direction of long axis of the teeth and crown surface have been observed on the verge of fracture at the 60 minutes interval. 3) $600^{\circ}F$ Groop: Crowns take on grayish-white color thoroughout 10, 30 and 60 minutes intervals and roots reveal black color. Moreover the seperation of enamel from dentin has been confirmed at the 60 minutes interval and inner dentin has changed black. 4) $800^{\circ}F$ Groop: Crowns take on the same grayish-white color as at the $600^{\circ}F$ at the 10, 30 and 60 minutes intervals and roots reveal gray color at the 30 and 60 minutes intervals, while parts of the crown have fractured at the 10 minutes interval. Inner dentin has turned gray at the 60 minutes interval.

계획된 행동이론을 적용한 초등학생의 칫솔질 행동에 영향을 미치는 요인

윤수길 ( Soo Gil Yoon ),한수진 ( Su Jin Han ) 한국치위생과학회 2014 치위생과학회지 Vol.14 No.4

This study was conducted to identify the factors influencing toothbrushing behaviors of elementary school students by adding the variable of ‘self-efficacy’ to the behavioral model based on the theory of planned behavior. To collect the data for an analysis, the self-administered questionnaire was distributed to 443 students in 4th to 6th grades of elementary school placed in Inchon Metropolitan City. The statistical package of PASW 18.0 was employed for the analysis. Results of the analysis revealed that the toothbrushing intention was explained by the subjective norm (b=0.343), the self-efficacy (b=0.171), the perceived behavioral control (b=0.163), and the attitude toward the tooth-brushing behavior (b=0.135). The toothbrushing behavior was explained by the toothbrushing intention (b=0.355) and the self-efficacy (b=0.113). The synthesis of such results suggested that both parents and teachers in school who would be influential to students should guide elementary school students to be habituated to the right toothbrushing. Also it was estimated that the students should understand the importance of toothbrushing through proper oral health education, and they should also become confident in spontaneous toothbrushing by providing them with an environment that could help them practicing the toothbrushing easily.

E1B-19k의 세포내 위치와 Bax와의 Dimerization에 관한 연구

윤수한,김진영,박승우,안영환,안영민,조기홍,조경기,Yoon, Soo Han,Kim, Jin Young,Park, Seung Woo,Ahn, Young Hwan,Ahn, Young Min,Cho, Ki Hong,Cho, Kyung Gi 대한신경외과학회 2000 Journal of Korean neurosurgical society Vol.29 No.6

Purpose : The subcellular localization of E1B-19k has been known cytosol or nuclear membrane by immunohistochemical staining and could dimerize with Bax to regulate cell death also known by the in-vitro immunoprecipitation. We planed to confirm this dimerization of E1B-19k with Bax in vivo in Cos-7 cells by using green fluorescent protein. Material and Method : We cloned E1B-19k and Bax into C3-EGFP. C3-EGFP-E1B-19k, C3-EGFP-Bax, and C3-EGFP-E1B-19k and pcDNA3-Bax were transfected into Cos-7 cells. We explored location of E1B-19k and Bax, and confirmed its dimerization with Bax in transfected living healthy Cos-7 cells by following green fluorescent protein of E1B-19k on the confocal microscope. Results : E1B-19k was located diffusely in cytoplasm and in nucleus but not in mitochondria. It prevented cell death from the apoptosis by staurosporine but its location was not changed. GFP-E1B-19k is not changed its intracellular location with Bax even with staurosporine. Conclusion : These results support that E1B-19k does not localize in mitochondria nor dimerize with Bax even with staurosporine. We could anticipate E1B-19k prevent cell death via the other dimerizing partner or pathways.

수두증을 동반한 거대두개증 환아에서 격자술을 이용한 One Stage Reduction Cranioplasty - 증례보고 -

원근수,윤수한,신용삼,조기홍,조경기,Weon, Keun Soo,Yoon, Soo Han,Shin, Yong Sam,Cho, Ki Hong,Cho, Kyung Gi 대한신경외과학회 2001 Journal of Korean neurosurgical society Vol.30 No.1

Reduction cranioplasty is one of the treatment modality among many treatment options for macrocephaly with hydrocephalus. The most previous techniques of reduction cranioplasty have some disadvantages such as difficult airway maintenance, pressure sore due to modified prone position, severe venous infarct due to obstruction of venous blood flow and large amount of bleeding from the dissection of superior sagittal sinus to obtain bone fragment needed. A 28-month-old girl had extreme macrocephaly. She couldn't control head rotation and keep sitting position. The operation was performed at supine position with adequate exposure of entire calvarium and the hinge was made in occipital bone fragment that covered posterior part of superior sagittal sinus. Bleeding volume and the other complications were decreased and acceptable reduction was achieved with this method.