http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Comparative functional venomics of social hornets Vespa crabro and Vespa analis
윤경재,김경문,Phuong Nguyen,서종복,박영한,김기영,서홍율,고영호,이시혁 한국응용곤충학회 2015 Journal of Asia-Pacific Entomology Vol.18 No.4
The hornets Vespa crabro and V. analis are widely distributed in Asia and are known to be aggressive when disturbed, resulting in frequent stinging accidents. To investigate the differences in venom properties and toxicities between these two hornets, the transcriptomic profiles of venom glands, in conjunction with the venom components, were analyzed and compared. A total of 35 venom-specific genes were identified in both venom gland transcriptomes, but their transcriptional profileswere different between V. crabro and V. analis. In addition, the major venom components were identified and confirmed by mass spectroscopy. Prepromastoparan, vespid chemotactic peptide precursor and vespakinin were the top three genes most prevalently transcribed in the venom gland of V. crabro, and their transcription rates were 112-, 16- and 161-fold higher, respectively, compared with those in V. analis, as judged by FPKMvalues. In the venomgland of V. analis, however, vespid chemotactic peptide precursor was the most abundantly transcribed gene, followed by premastoparan and vespakinin. In general, most major venom genes were more abundantly expressed in V. crabro, whereas some minor venom genes exhibited higher transcription rates in V. analis, including muscle LIM protein, troponin, paramyosin, calponin, etc. Our findings reveal that the overall venom components of V. crabro and V. analis are similar, but that their expression profiles and levels are considerably different. The comparison of venom gland transcriptomes suggests that V. crabro likely produces venom with more highly enriched major venom components, which has potentially higher toxicity compared with V. analis venom
손목 터널 증후군에서 하이드록소코발라민 근주 효과: 무작위 대조 연구
윤경재,이용택 대한근전도전기진단의학회 2013 대한근전도 전기진단의학회지 Vol.15 No.1
Objectives: To evaluate the effect of intramuscular administration of hydroxocobalamine for the carpal tunnel syndrome (CTS) on clinical symptoms and electrophysiologic changes. Subjects and Methods: 16 CTS patients were randomly assigned to hydroxocobalamine (HC) or sham group. HC group received intramuscular hydroxocobalamine (5 mg) 10 times for 20 days. Tinel score, Phalen time, Levin Questionnaire and nerve conduction study were measured 1 month after injection schedule. All patients were treated with ultrasound during trial period. Results: Total six patients (three in each group) completed follow-up. After one month, Tinel score, Phalen time, Levin Questionnaire and nerve conduction study showed no significant difference between two groups. Conclusion: Intramuscular hydroxocobalamin did not have beneficial effect on CTS for 1 month followup period. However, further data should be collected to conclude the effect of hydroxocobalamin on CTS.
Comparative bioactivities of mastoparans from social hornets Vespa crabro and Vespa analis
윤경재,김경문,Phuong Nguyen,서종복,박영한,김기영,서홍율,고영호,이시혁 한국응용곤충학회 2015 Journal of Asia-Pacific Entomology Vol.18 No.4
Vespa crabro and V. analis are social hornet species commonly found in Asia, including Korea.Mastoparan is one of themajor venompeptides of these two hornets but its amino acid sequence defers substantially. To examine the differences in the potential toxicity and bioactivity ofmastoparans between these two social hornets, differential toxicological and pharmacological activities of synthesized mastoparan were investigated. V. analis mastoparan showed a 7-fold higher hemolytic activity, suggesting its higher cytotoxic potential compared with V. crabro mastoparan. Mastoparans from both hornet species exhibited similar levels of antimicrobial activities against Staphylococcus aureus and Botrytis cinerea, whereas the mastoparan from V. analis showed more potent antimicrobial activities against Escherichia coli and Candida albicans. Nevertheless, the antimicrobial activities of mastoparans of V. crabro and V. analis were relatively lower compared with those of other wasps. Both mastoparans also exhibited some levels of antitumor activity but the activity was significantly higher in V. analis mastoparan. In summary, the hemolytic, antimicrobial, and antitumor activities of synthesized V. analis mastoparan were higher than those of V. crabro mastoparan. These differential bioactivities are likely due to the amino acid sequence differences in the mature peptides. In particular, the additional Lys residue present in V. analis mastoparan may contribute to the higher levels of bioactivity as proposed by secondary structure prediction.
Cellulase gene expression profiles in termites according to habitat and diet
윤경재,김주현,황채은,김영호,이원훈,이시혁 한국응용곤충학회 2015 Journal of Asia-Pacific Entomology Vol.18 No.3
To investigate the expression profiles of cellulase genes in termites due to differences in habitat and diet, we collected one species (Microtermes pakistanicus) fromdrywood, one species (Macrotermes gilvus) fromrotten damp wood and one species (Microtermes sp.) from cow dung. Total RNA was extracted from the hind guts containing paunch and fromother tissues fromeach collected species and used for the suppression subtractive hybridization to enrich cellulase genes. The resulting EST librarieswere sequenced, the cellulase geneswere identified by BLAST search, and their phylogenetic relationships were determined. Depending on habitat and diet, termites exhibited different expression profiles of cellulase genes. Endo-β-1,4-glucanase (glycosyl hydrolase family 9, GHF 9) was the predominant cellulase expressed in M. pakistanicus,which consume drywood. In contrast,M. gilvus, consuming rotten damp wood, utilized cellobiohydrolase (GHF 7) as the major cellulase. Additionally, β-glucosidase, a cellobiase (GHF 1), was found to be the major cellulase in Microtermes sp., which consume cow dung. Based on these findings and the supposition that raw cellulose materials are likely preprocessed by cellulolytic microorganisms in damp wood and cow dung, we hypothesized that termites consuming these preprocessed cellulose materials (i.e., cellobiase) have adapted to producemore exocellulases than endocellulases. This notionwas supported by the fact that endo-β-1,4-glucanase (i.e., endocellulase) was predominantly expressed only in M. pakistanicus, which consume the raw cellulose of drywood. An analysis of the catalytic domains of the cloned cellulases suggested that these cellulases possess enzymatic activity.
철근강화콘크리트에 대한 받음각이 없는 관통자의 수직관통거동 전산해석
윤경재,유요한,김학준,Yun, Kyung Jae,Yoo, Yoo-Han,Kim, Hak Jun 한국군사과학기술학회 2013 한국군사과학기술학회지 Vol.16 No.3
The simulation of the ballistic trajectory of penetrator into the spaced multi-layer RC targets is very important to predict the hitting condition in subsequent target. Because of perturbation by lateral load of penetrator caused by asymmetric hitting position between penetrator and steel bar reinforcement, penetrator rotates and deviates from the straight path. Therefore, penetration capability of penetrator is decreased in the subsequent targets. This paper presents the result of the penetration of steel-bar-reinforced concrete target by using the explicit finite element code LS-DYNA. A series of computations is performed and compared to experimental data and the computed results are in good agreement with the experimental results over a wide range of velocities. And then we conduct the simulation according to various RC target hitting condition and impact velocities.
외부 폭발에 의한 축소형 방폭문 변형 및 터널 내부 폭풍파 전파 거동의 수치해석
윤경재,유요한,Yun, Kyung Jae,Yoo, Yo-Han 한국군사과학기술학회 2016 한국군사과학기술학회지 Vol.19 No.4
In this paper, we present the results of the numerical analysis employing CONWEP, LS-DYNA FSI(Fluid Structure Interaction), AUTODYN FSI, LS-DYNA ALE(Arbitrary Lagrange Eulerian) and combination of CONWEP and LS-DYNA ALE for blast door fracture and wave propagation through the tunnel by the external explosion. We compared the numerical analysis results with the subscale test data and selected combination of CONWEP and LS-DYNA ALE method as adequate data generation method for the FRM(Fast Running Model) software development. It is expected to save much time and costs by using the numerical simulation data for the various test conditions.
윤경재,유홍희 한국소음진동공학회 2000 소음 진동 Vol.10 No.6
This paper presents a modeling method for the vibration analysis of rotating cantilever beams considering the elastic foundation effect. Mass and stiffness matrices are derided explicitly by considering coupling effect between stretching and bonding motion. Numerical results show that the bending direction elastic foundation stiffness influences the vibration characteristics significantly in practical range of beam configuration. The ranges of elastic foundation stiffness to avoid the dynamic buckling are also presented. The method presented in this paper can be used to predict the variations of natural frequencies of rotating cantilever beams with elastically restrained root.