Nitric Oxide 의 합성 억제제인 N-Nitro-L-Arginine 의 항이뇨작용 기전에 관한 연구

고석태(Suk Tai Ko),유강준(Kang Jun Yu) 한국응용약물학회 1998 Biomolecules & Therapeutics(구 응용약물학회지) Vol.6 No.3

This studies were performed for investigation of mechanism on central antidiuretic action of L^G-Nitro-L-arginine (L-NOARG), nitric oxide synthase inhibitor, in dog. Antidiuretic action of L-NOARG infused into the carotid artery was not affected by renal denervation but inhibited by pretreatment with arginine, NO precusor. Furthermore, L-NOARG inhibited the diuretic action of dopamine induced by hemodynamic development. Above results suggest that antidiuretic actions of L-NOARG mediated by endogenous substances not associated with renal nerve. Therefore, it is demonstrated that those endogenous substances might be associated with NO which mediate the diuretic action of dopamine.

니트릭옥사이드의 합성 억제제인 NG-니트로-L-아르기닌의 신장작용

고석태(Suk Tai Ko),유강준(Kang Jun Yu),황명성(Myung Sung Hwang) 大韓藥學會 1998 약학회지 Vol.42 No.5

This study was performed in order to investigate the effect of renal function of NG-nitro-L-arginine (L-NOARG), inhibitor of nitric oxide (NO) synthase, in dog and rabbit. L-NOARG, when given intravenously in dogs, exhibited the decrease in urine flow (vol), renal plasma flow (RPF), osmolar clearance (Cosm) and amounts of sodium and potassium excreted in urine(ENa, EK). These renal functions of L-NOARG showed the same aspect in rabbit, too. L-NOARG, when administered into a renal artery, showed the same pattern as was obtained when given intravenously in both experimental and control kidney in dog. L-NOARG administered into the carotid artery showed the decrease in Vol, RPF, ENa, in a low doses that did not show any effect when given intravenously. Above results suggest that L-NOARG produces antidiuretic action in dog and rabbit, and these antidiuretic actions may be mediated by central action.

한쪽 신동맥내 Debrisoquin 의 항이뇨작용기전

고석태(Suk Tai Ko),유강준(Kang Jun Yu),신동숙(Dong Sook Shin),이수연(Soo Hyan Lee) 한국응용약물학회 1995 Biomolecules & Therapeutics(구 응용약물학회지) Vol.3 No.2

This study was performed in order to certify the antidiuretic action and to investigate the mechanism of antidiuretic action of debrisoquin infused into a renal artery in dog. Debrisoquin, when infused into a renal artery, exhibited the antidiuretic action accompanied the reductions of glomerular filtration rate and renal plasma flow, and the decreased amounts of sodium and potassium excreted in urine, limited only to the infused side, while control kidney function remained unchanged at all. The antidiuretic action of debrisoquin infused into a renal artery was blocked by pretreament of prazosin, α₁-adrenergic blocking agent, or reserpine, catecholamine depleting agent. These results suggest that debrisoquin infused into a renal artery elicits antidiuretic action through indirect stimulation of renal sympathetic nerves.

애엽 추출분획 , DA-9601의 일반 약리작용

고석태(Suk Tai Ko),유강준(Kang Jun Yu),김순회(Soon Hoe Kim),이은방(Eun Bang Lee),천선아(Seon Ah Cheon),신동숙(Dong Sook Shin),이은심(Eun Shim Lee),김옥경(Ok Kyung Kim),강선영(Seon Young Kang),손문호(Moon Ho Sohn) 한국응용약물학회 1996 Biomolecules & Therapeutics(구 응용약물학회지) Vol.4 No.2

The general pharmacological properties of Artemisia extract powder (DA-9601) produced from Artemisia asiatica leaves were investigated in mice, rats, guinea pigs and rabbits. DA-9601 at the dose of 800 ㎎/㎏ po had no influences on general behaviour, barbital sleeping time and motor coordination of mice. The material at the oral dose of 800 ㎎/㎏ did exhibit neither analgesic action nor hypothermic effect. Anticonvulsant action, muscle relaxant action and the effect on intestinal propulsion were not identified at 800 ㎎/㎏ po. In the isolated ileum and trachea of guinea pig, the material did not show direct effect and inhibitory action of chemically or electrically stimulated contraction at the concentration of 2 x 10^(-5) g/㎖ The sinus rates of atria and contractility of papillary muscle of guinea pig were not influenced by DA-9601 at a dose of 2 x 10^(-5) g/ml. No influences on blood pressure and respiration were observed at 40 ㎎/㎏ iv, in rabbits. However, transient decreases in blood pressure of rabbits were observed as given 120 ㎎/㎏ in iv route with slight respiratory depression, and slight diuretic effect could be found without any changes in Na^+ and K^+ excretion.

Methoxyverapamil 의 신장작용에 미치는 신 신경제거의 영향

고석태(Suk Tai Ko),이수정(Soo Jeong Lee),유강준(Kang Jun Yu) 한국응용약물학회 1994 Biomolecules & Therapeutics(구 응용약물학회지) Vol.2 No.3

In dogs, renal denervation did not affect the diuretic action accompanied with renal hemodynamic changes and inhibition of electrolytes reabsorption rates in renal tubules by methoxyverapamil infused into the vein or into a renal artery, while renal denervation blocked the antidiuretic action due to the decreased free water and osmolar clearances along with the reduced sodium amounts excreted in urine by methoxyverpamil infused into the carotid artery. These experimental results suggest that methoxyverapamil may cause diuresis by direct action in kidney but the antidiuretic action through central function mediated by renal nerves.

나프록센의 항이뇨작용 기전

고석태(Suk Tai Ko),이한구(Han Koo Lee),유강준(Kang Jun Yu) 대한약학회 1995 약학회지 Vol.39 No.3

This study was attempted to investigate the mechanism of retention of sodium and water by naproxen which is a drug among nonsteroidal anti-inflatnmatory drugs in dogs. Napoxen, when given intravenously in doses ranging from 30mg to 100mg/kg, elicited antidiuresis accompanied with the decrease of osmolar clearance(Cosm) and amounts of sodium excreted in urine(ENa), with the increase of sodium reabsorption rate in renal tubule(RNa) and ratio of potassium against sodium (K/Na). Naproxen infused into a renal artery in doses ranging from 1.Omg to 3.Omg/kg/min produced both diuretic action in infused kidney and antidiuretic action in control kidney. Naproxen injected into carotid artery in doses ranging from 1O.Omg to 30.Omg/kg exhibited antidiuretic action. Changes of renal function in the circumstances of above two antidiuresis were the same with aspect of intravenous naproxen. Antidiuretic action of naproxen injected into carotid artery was not affected by renal denervation, was blocked by pretreatment with i.v. arachidonic acid, prostaglandin precursor, or i.v. indomethacin, cyclooxygenase inhibitor. Naproxen injected into carotid artery abolished the diuretic action of i.v. spironolactone, aldosterone antagonist, and i.v. spironolactone blocked the antidiuretic action of naproxen given into carotid artery. The results suggest that naproxen produced antidiuresis, and sodium and water retention through the central system, the mechanism being related to the prostaglandin biosynthetic inhibition and aldosterone like action.

바소프레신의 이뇨작용

고석태(Suk Tai Ko),윤재경(Jae Kyoung Yun),유강준(Kang Jun Yu) 대한약학회 1996 약학회지 Vol.40 No.4

Vasopressin which is an antidiuretic hormone in human body produced the diuretic action in dog. This study was investigated in order to certify the diuretic action and to search out the mechanism of the action on the vasopressin. Vasopressin, when given in a dose of 10.0mU/kg, bolus+1.0mU/kg/min intravenously, exhibited the increase of urine flow(Vol), renal plasma flow(RPF), osmolar clearance (Cosm) and amounts of sodium and potassium excreted in urine (ENa, EK), the decrease of reabsorption rate of sodium and potassium in renal tubules (RNa, RK), and then elevated the mean arterial pressure(MAP). Vasopressin given in a increased dose to 30.0mU/kg, bolus+1.0mU/kg/min intravenously elicited the same aspect with that exhibited by a small dose in changes of Vol. and all renal function and potentiated the change rates, whereas this time MAP did not change at all when compared with control value. Vasopressin, when administered into a renal artery, did not induce the changes of Vol and all renal function in experimental (administered) kidney, but increased slightly the Vol, glomerular filtration rate(GFR), ENa and EK expected the no change of RNa and RK in the control (not administered) kidney. Vasopressin, when infused into carotid artery, showed the increase of Vol. GFR, ENa, EK and no change of RNa and RK in a dose of 1/5 of intravenous dose. Diuretic action of vasopressin administered into carotid artery was not influenced by renal denervation. Above results suggest that vasopressin produced diuretic action by hemodynamic changes in dogs. These hemodynamic changes may be mediated by central endogenous substances not associated with renal nerve.

Ditiazem 의 신장작용에 대한 신신경제거의 영향

고석태,김해석,유강준 한국응용약물학회 1993 Biomolecules & Therapeutics(구 응용약물학회지) Vol.1 No.1

This study was performed to elucidate the mechanism of antidiuretic action of diltiazem by infusion into the vein and carotid artery, of diuretic action into a renal artery in dog. Renal denervation caused a reversal of the effect of diltiazem from the antidiuretic to the diuretic when infused into vein or carotid artery, and potentiated the diuretic effect when infused into a renal artery. The changes of renal function in diuretic circumstances as described above included the increase in renal plasma flow, osmolar clearance, the amounts of sodium and potassium excreted in urine and the decrease in reabosrption rate of sodium and potassium in renal tubules. Above results suggest that antidiuretic action of diltiazem may be mediated by central nervous system, not by endogenous substance, diuretic action by direct renal action.

바소프레신의 이뇨작용

고석태,윤재경,유강준 朝鮮大學校 1997 藥學硏究誌 Vol.18 No.2

Vasopression which is antidiuretic hormone in human body produced the diuretic action in dog. This atudy was investigated in order to certify the diuretic action and to search out the mechanism of the action on the vasopression. Vasopressin, when given in a dose of 10.0mU/㎏, bolus+1.0mU/㎏/min intravenously, exhibited the increase of urine flow(Vol), renal plasma flow(RPF), osmolar clearance(Cosm) and amounts of sodium and potassium excreated in urine(E_Na, E_K), the decrease of reabosorption rate of sodium and potassium in renal tubules(R_Na, R_K), and then elevated the mean arterial pressure(MAP). Vasopressin given in a increased dose to 30.0 mU/㎏,bolus+1.0mU/㎏/min intravenously elicited the same aspect with that exhibited by a small dose in changes of Vol. and all reansl fuction and potentiated the change rates, whereas this time MAP did not change at all when compared with control value. Vasopressin, when adminstered into a renal artery, but increased slightly the Vol, glomeruler filtration rate(GFR). E_Na and E_K excepted the no change of R_Na and R_K in the control (not administered)kidney. Vasopressin. when infused into carotid artery, showed the increase of Vol. GFR,E_Na, E_K and no change of R_Na and R_K in a dose of 1/5 of intravenouse dose. Diuretic action of vasopressin administewred into carotid artery was not influenced by renal denervation. Above results suggest that vasopressin produces diuretic action by hemodynamic changes in dog. These hemodynamic changes may be mediated by central endogenous substances not associated with renal nerve.

Enalapril이 개의 賢臟機能에 미치는 影響

高錫太,金海石,柳康俊 조선대학교 약학연구소 1993 藥學硏究誌 Vol.15 No.1

Enalapril, angiotensin converting enzyne(ACE) inhibitor, when injected into the vein in dog, produced diuretic action accompanied with the icrease of renal plasma flow(RPF), glomerular filtration rate (GFR), osmolar substance clearace(Cosm) and excretion rates of sodium and potassium in urihne(E_Na, E_K), but in this time, reabsorption rates of sodium and potassium in renal tubules(R_Na, R_K) were not changed at all. Enalapril, when infused into carotid artery, exhibited the same aspect with changes of renal function by intravenous enalapril. Enalapril infused into a renal artery did not affect the renal function of experimental kidney as a matter of control kidney. Above resalts suggest that enalapril produced diuretic action by renal hemodynamic inprovement through the central function