재조합 인간 상피세포 성장인자(DWP 401)의 일반약리작용

천선아(Seon Ah Cheon),김상미(Sang Mee Kim),이은방(Eun Bang Lee),임승욱(Seung Wook Lim),유영효(Young Hyo Yu),박명환(Myung Hwan Park) 대한약학회 1995 약학회지 Vol.39 No.5

The recombinant human epidermal growth factor(DWP 401) was investigated on the pharmacological actions. DWP 401 had no effects on the hexobarbital-induced sleeping time, locomotor activity, rotarod test, body temperature, analgesic action and anticonvulsant action in mice. It also had no influences on the isolated tracheal muscle and ileum of guinea-pig, isolated uterus and fundus strip of rats. Slight hypotensive action with effect on respiration was revealed at a dose of 8g/kg i.v. of DWP 401 in rabbits. DWP 401 exhibited a weak inhibitory action of glucose tolerance in normal rats, significantly lowered the blood glucose contents in adrenalectomized rats at a concentration of 160g/kg, and produced a significant inhibitory effect on leucocyte migration in CMC-pouch of rats at a concentration of 32g/rat. Furthermore, DWP 401 showed a significant decrease on gastric juice volume and acidity. However, DWP 401 had no intestinal propulsion rate and influence on urine excretion.

백두옹의 소염진통작용

천선아(Seon Ah Cheon),최병기(Byung Kee Choi),김성연(Seung Yeun Kim),이은방(Eun Bang Lee) 한국응용약물학회 2000 Biomolecules & Therapeutics(구 응용약물학회지) Vol.8 No.3

The root extract of pulsatilla koreana has been used as antibacterial, antiparasitic and anti-inflammatory analgesic agents in Traditional Medicine in Korea. Thus anti-inflammatory and analgesic actions of the methanol and water extracts of the root were investigated by administration in oral and intravenous route. From the results, it is concluded that the extracts exhibited the potent anti-inflammatory and analgesic actions in intravenous administration, but did not show the actions in oral administration in animals.

백두옹엑스 분획물의 소염진통작용

천선아,최병기,정춘식,이대위,이은방,Cheon, Seon-Ah,Choi, Byung-Kee,Jeong, Choon-Sik,Li, Da-Wei,Lee, Eun-Bang 한국생약학회 2000 생약학회지 Vol.31 No.2

From our previous report, the water extract of Pulsatilla koreana root was found to have potent anti-inflammatory and analgesic actions in intravenous administration in animals. Among chloroform, ethyl acetate, butanol and water fractions which were obtained through successive fractionation of the extract, only the water fraction was found to have the antiinflammatory and analgesic actions. The fraction did not affect normal body temperature at the effective doses in mice and showed low acute toxicity of which $LD_{50}$ was less than 500 mg/kg i.v. in mice. It is interesting that its anti-inflammatory action might be attributed in part to inhibition of cyclooxygenase-1 and -2.

재조합 인간 상피세포 성장인자(DWP 401)의 흰쥐 위액분비 및 궤양에의 작용

이은방(Eun Bang Lee),천선아(Seon Ah Cheon),이은심(Eun Shim Lee),김옥경(Ock Kyung Kim) 대한약학회 1996 약학회지 Vol.40 No.4

The effects of human epidermal growth factor(EGF) which was produced by recombinant DNA technique was investigated on gastric secretion, gastric lesion and ulcer models in rats. The EGF showed significant inhibition of secretion of gastric juice and total acid output, at 0.4mg/kg, id and also inhibited Shay ulceration at 0.4mg/kg, id in rats. The lesion induced by absolute ethanol was significantly reduced by oral administration of EGF at 0.4mg/kg. Likewise, EGF caused significant inhibition of indomethacin induced gastric ulcer at oral doses of 0.2 and 0.4mg/kg. The EGF produced dose-dependent inhibition of gastric ulcer induced by acidified aspirin, but showed no significant inhibition at oral doses of 0.1, 0.2 and 0.4mg/kg. The chronic gastric ulcer induced by injection of 20% acetic acid solution was significantly reduced by oral doses of 0.1 and 0.4mg/kg of EGF. Duodenal ulcer induced by mepirizole was dose-dependently inhibited by oral doses of 0.1, 0.2 and 0.4mg/kg of EGF. These data suggest that EGF possesses pronounced inhibitory action in gastric ulcer and duodenal ulcer of rats.

유전자 재조합 Erythropoietin (LB-00014) 의 일반약리작용

이은방(Eun Bang Lee),천선아(Seon Ah Cheon),이향주(Hyang Joo Lee),조성익(Sung Ig Cho),손지영(Jee Young Sohn) 한국응용약물학회 1996 Biomolecules & Therapeutics(구 응용약물학회지) Vol.4 No.2

General pharmacological properties of LB-00014, an erythropoietin which was produced by recombinant DNA technique in Biotech Research Institute, LG Chemical Ltd. were examined. The administration of LB-00014 (60, 600, 6000 IU/㎏, iv) in mice had no effect in general behavior and central nervous system, and no influences on normal body temperature, writhing syndromes induced by 0.7% acetic acid solution and chemoshock produced by strychnine and pentetrazole solution. LB-00014 (60, 600, 6000 IU/㎏, iv) given to anesthetized rabbits showed no effect on blood pressure of carotid artery and respiration rates, and it did not influence the responses produced by injection of acetylcholine or epinephrne. The administration of LB-00014 (601, 600, 6000 IU/㎏, iv) in rats had no effect on the plasma prothrombin time, activated partial thromboplastin time and hemolytic action. The platelet aggregation induced by collagen in human plasma was not influenced by LB-00014 (10, 100, 1000 IU/㎏, iv). It showed no direct effect at 100 and 1000IU/ml in isolated stomach fundus and uterus of rats and ileum of guinea-pig, and it also had no inhibition of contraction produced by acetylcholine, oxytocin, serotonin and histamine in the above-mentioned preparations. It did not influence gastric secretion, pH and acid output at 6000 IU/㎏, iv in rats, but showed a significant increase in intestinal propulsion of mice at 6000 IU/㎏, iv. Its administration (60, 600, 6000 IU/㎏, iv) caused no effect on urine and electrolyte excretion in rats. These results indicate that LB-00014 does not exsert any of serious pharmacological effects.

재조합 부갑상선 홀몬의 일반약리작용

이은방(Eun Bang Lee),장혜옥(Hae Ock Chang),이향주(Hyang Joo Lee),천선아(Seon Ah Cheon) 한국응용약물학회 1998 Biomolecules & Therapeutics(구 응용약물학회지) Vol.6 No.2

General pharmacological properties of recombinant human parathyroid hormone (hPTH) were examined. The administration of hPTH (7, 35 and 175 IU/kg sc) in mice had no effects in general behavior and central nervous system, and no influences on normal body temperature, writhing syndromes induced by 0.7% acetic acid solution and chemoshock produced by strychnine and pentetrazol solution. hPTH (9 and 44 IU/kg, sc) given to anesthetized rabbits showed no effect on blood pressure of carotid artery and respiration, and it did not influence the responses produced by injection of acetylcholine or epinephrine. It showed no direct effect at 4.4 x 10^(-2) IU/ml in isolated stomach fundus and uterus of rats and ileum of guinea-pig, and it also showed no inhibition of contraction produced by acetylcholine, oxytocin, serotonin and histamine in the above-mentioned preparations. It did not influence intestinal propulsion at the doses of 7, 35 and 175 IU/kg sc in mice. This drug exhibited no effect on urinary excretion at the doses of 7 and 35 IU/kg sc in rats. However, at a dose of 175 IU/kg sc, it showed a decreased urination along with decreased excretion of potassium, sodium and chloride ion. These results indicate that hPTH does not exert any of serious pharmacological effects except the inhibition of urination at a highest dose used.

애엽 추출분획 , DA-9601의 일반 약리작용

고석태(Suk Tai Ko),유강준(Kang Jun Yu),김순회(Soon Hoe Kim),이은방(Eun Bang Lee),천선아(Seon Ah Cheon),신동숙(Dong Sook Shin),이은심(Eun Shim Lee),김옥경(Ok Kyung Kim),강선영(Seon Young Kang),손문호(Moon Ho Sohn) 한국응용약물학회 1996 Biomolecules & Therapeutics(구 응용약물학회지) Vol.4 No.2

The general pharmacological properties of Artemisia extract powder (DA-9601) produced from Artemisia asiatica leaves were investigated in mice, rats, guinea pigs and rabbits. DA-9601 at the dose of 800 ㎎/㎏ po had no influences on general behaviour, barbital sleeping time and motor coordination of mice. The material at the oral dose of 800 ㎎/㎏ did exhibit neither analgesic action nor hypothermic effect. Anticonvulsant action, muscle relaxant action and the effect on intestinal propulsion were not identified at 800 ㎎/㎏ po. In the isolated ileum and trachea of guinea pig, the material did not show direct effect and inhibitory action of chemically or electrically stimulated contraction at the concentration of 2 x 10^(-5) g/㎖ The sinus rates of atria and contractility of papillary muscle of guinea pig were not influenced by DA-9601 at a dose of 2 x 10^(-5) g/ml. No influences on blood pressure and respiration were observed at 40 ㎎/㎏ iv, in rabbits. However, transient decreases in blood pressure of rabbits were observed as given 120 ㎎/㎏ in iv route with slight respiratory depression, and slight diuretic effect could be found without any changes in Na^+ and K^+ excretion.

영지의 단백다당체 G009 의 일반약리작용

정훈(Hoon Jeong),이승룡(Seung Yong Lee),김수웅(Su Ung Kim),한만덕(Man Deuck Han),이은방(Eun Bang Lee),천선아(Seon Ah Cheon),김상미(Sang Mee Kim),김경란(Kyung Ran Kim),이승목(Seung Mok Lee),현익상(Ik Sang Hyun),이준우(June Woo Lee) 한국응용약물학회 1994 Biomolecules & Therapeutics(구 응용약물학회지) Vol.2 No.4

A polysaccharide, G009, isolated from Ganoderma lucidum IY 009, was subjected to investigating on general pharmacology. This material at the large oral doses of 1000 and 2000 ㎎/㎏ in mice did not exhibit any abnormal behaviors and another effects on central nervous system. It also had no influences on hexobarbital-induced sleeping time, rotarod test and spontaneous activity test at each oral dose of 1000 ㎎/㎏ in mice. No effects on the body temperature and on acetic acid induced writhing syndrome in mice were observed with its oral administration at 1000 ㎎/㎏, and the convulsions induced by strychnine and pentetrazole were not inhibited at its oral doses of 1000 ㎎/㎏ in mice. The solution of G009 as given intravenously at the doses of 30 and 60 ㎎/㎏ in rabbit had no influences on blood pressure and respiration rates and depth. In isolated organs of rat uterus and fundus muscles and guinea pig ileum and trachea, it did not show any contraction or relaxation at the concentrations of 2 X 10^(-3) g/㎖, and the contractive actions produced by oxytocin, acetylcholine, serotonin and histamine were not inhibited at the same doses. This material showed no effect on intestinal propulsion test in mice and gastric secretion in rats at the oral doses of 1000 ㎎/㎏. However, it is interesting that the material exhibited potent inhibition of acidified aspirin induced gastric damage at the doses of 500 and 1000 ㎎/㎏ in rats.

효모에서 발현된 유전자 재조합 탈메치오닌 인간 성장호르몬의 일반 약리작용

이은방(Eun Bang Lee),신국현(Kuk Hyun Shin),김운자(Oon Ja Kim),윤기영(Ki Young Yoon),천선아(Seon Ah Cheon),채윤정(Yun Jung Chae) 대한약학회 1992 약학회지 Vol.36 No.1

The general and some other pharmacological actions of growth hormone without N-terminal methionine(rhGH) were investigated in animals. The hormone had no influenced on the central nervous system and on body temperature at a high oral dose of 40IU/kg in animals. It had neither analgesic nor antiepileptic actions at the High doses. In the isolated ileum and trachea of guinea-pig and isolated stomach fundus and uterus of rat, it showed neither contractive nor relaxing effets at a concentration of 1 X 10-3IU/ml in bath, and no inhibitory action at a dose of 1 X 10-3IU/ml against the contractions produced by histamine (5 X 10-5g/ml), serotonin(1 X 10-5g/ml, acetylcholine(1 X 10-5g/ml) and oxytocin(5 X 10-3IU/ml). Furthermore, the intravenous infection of 20IU/kg rhGH had no influences on the normal blood pressure and respiration in rabbits. These negative results in pharmacological profile are thought that the hormone may not elicit serious side effects On the other hand, the rhGH exhibited a weak inhibitory action of glucose tolerance in normal rats, significantly lowered the blood glucose contents in adrenalectomiglucose tolerance in normal rats, significantly lowered the blood glucose contents in adrenalectomized rats 20 min after i.v. administration of 80IU/kg, and showed a significant inhibitory effect on in vitro glycerol release in epinephrine-stimulated epididymal fat pad segments of rats.

애엽 추출분획, DA-9601의 일반 약리작용

이은방,천선아,이은심,김옥경,고석태,유강준,신동숙,강선영,김순회,손문호 朝鮮大學校 1997 藥學硏究誌 Vol.18 No.2

The general pharmacological properties of Artemisa extract power(DA-9601) produced from Artemisa asiatica leaves were investigated in mice, rats, guinea pigs and rabbits. DA-9601 at the dose of 800㎎/㎏ po had no influences on general behaviour, barbital sleeping time and motor corrdination of mice. The material at the oral dose of 800㎎/㎏ did exhibit neither analgesic action nor hypothermic effect. Anticonvulsant action, muscle relaxant action and the effect on intestinal propulsion were not identified at 800㎎/㎏ po. In the isolated ileum and trachea of guinea pig, the material did not show direct effect and inhibitory action of chemically or electrically stimulated contraction at the concentration of 2×10^-5 g/ml. The sinus rates of atria and contractility of papillary muscle of guinea pig were not influenced by DA-9601 at a dose of 2×10^-5 g/ml. No influences on blood pressure and respiration were observed at 40㎎/㎏ iv, in rabbits. However, transient decreases in blood pressure of rabbits were observed as given 120㎎/㎏ in iv route with slight respiratory depression, and slight diuretic effect could be found without any changes in Na^+ and K^+ excretion.