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Cunninghamella blakesleeana IFO 4443의 변이주로부터 키토산의 생산
류병호(Beung-Ho Ryu),김희숙(Hee-Suck Kim),원용돈(Yong-Don Won),임복규(Bok-Gu Lim) 한국식품영양과학회 1997 한국식품영양과학회지 Vol.26 No.1
키토산을 생산하기 위하여 Cunninghamella blakesleeana IFO 4443을 자외선조사하여 생성능이 우수한 Cunninghamella blakesleeana IFO 4443-10의 변이주를 분리하였다. 본 변이주인 C. blakesleeana IFO 4443-10을 30℃에서 pH 4.5에서 3일간 발효하여 mycelia 발효액을 알칼리로 처리하여 chitosan을 얻었다. 이때 chitosan의 수율은 1012㎎/l로 높았으며 탈아세틸화는 84.6%이였다. A method for lab scale production and isolation of chitosan from mycelia of Cunninghamella blakesleeana IFO 4443 mutant was developed. Mutant of Cunninghamella blakesleeana IFO 4443-10 obtained by UV radiation was cultivated in 5L jar fermentor at 30℃ for 3days. The fungus were grown well at pH 4.5. Chitosan was readily extracted from mycelia walls with alkali treatment. The maximum yield of chitosan obtained was 1012㎎/L and degree of deacetylation was 84.6%.
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Thiobacillus neapolitanus R-10 에 의한 유황계 악취물질의 제거
원용돈,박상보,이원구,송승구 한국환경과학회 1995 한국환경과학회지 Vol.4 No.2
Thiobacillus neapolitanus R-10 isolated from sludge of night soil, showed an oxidizing activity on several malodorous sulfur compounds. The microbe successfully utilized hydrogen sulfide(H_2S), methy mercaptan(MM), dimethyl sulfide(DMS) and dimethyldisulfide(DMDS) during the batch culture reaction, of which H_2S was rather rapidly oxidized. To examine the ability for removal of malodorous sulfur compounds, various concentrations of sulfide substrates were supplemented separately to basal medium and their responses were investigated. As the concentration of sulfide was increased, growth was accelerated within three days of cultivation. 2.5mM was the most favorable substrate concentration of sulfide added for all cases tested. However, when the concentration of sulfur compounds were raised over 4mM, they behaved as a growth inhibitor.
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김희숙,원용돈,류병호 한국식품영양학회 1996 韓國食品營養學會誌 Vol.9 No.4
기능성 다이어트 식품을 개발을 목적으로, 키토산과 견 피브로인의 복합체를 만들고 성질을 조사하였다. 키토산은 대동새우로부터 추출하였고, 견사 피브로인 섬유는 누에로부터 추출하였다. 견사의 특성을 알아보기 위하여 용해도를 조사한 결과, 용해도는 8M LiBr이상의 농도와 40℃ 이상에서 가장 높았다. 견사 피브로인의 아미노산 조성은 천연견사 및 용해시킨 피브로인과 같았다. 키토산, 견사 피브로인 복합체의 아미노산 조성은 glycine, alanine, serine, tyrosine, threonine 및 glutamic acid 순서를 많았다. 또한 IR 스펙트럼 결과, 키토산·견사 피브로인 복합체는 배합비율에 따른 스텍트럼상의 차이는 거의 없었다. In order to develop the functional diet food, characteristics of chitosan and silk fibroin composite was designed. Chitosan extracted from a prawn and silk fibroin was prepared from silkworm. The silk fibroin was dissolved rapidly in the 8M LiBr at a temperatrue of more than 40℃. Amino acid composition of fibroin composite revealed the same pattern that of native silk fibroin and regenerated silk fibroin. Predominant amino acid of chitosan-fibroin composite contained glycine, alanine, serine, tyrosine, threonine, and glutamic acid in order. According to the basis on the infrared spectrum, chitosan-silk fibroin composite is not distinguished differents composite ratio of chitosan and silk fibroin.
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금속/클로로프렌 고무/플라스틱 접착 시편에서 금속의 표면처리가 접착정도에 미치는 영향
최홍석,박성수,원용돈 釜慶大學校 1999 釜慶大學校 論文集 Vol.4 No.1
To investigate the change of adhesive characteristics by surface modification of metal, peel strength of adhesive samples was measured using an universal testing machine(UTM). The adhesive samples were fabricated by bonding with chloroprene rubber adhesive between various plastic sheets (PE, PP, PS, and PVC) and various surafce modified metal sheets (steel sheet, blasted metal, Al₂O₃plasma coated metal, Al₂O₃/TiO₂plasma coated metal, and ZrO₂/Y₂O₃plasma coated metal). To explain the trend of peel strength, surface roughness, contact angle, and Vickers hardness of various surface modefied metals were measured. With increasing surface roughness and/or decreasing contact angle in various surface modified metals, the peel strength of adhesive sample increased. It was assumed that higher surface roughness and/or lower contact angle increased bonding area of adhesive.
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