환경시료 중 $^{90}Sr$의 분석을 위한 분리농축법 연구

원미숙,조규봉,윤장희,이대원,심윤보,Won, Mi Sook,Cho, Kyu Bong,Yoon, Jang Hee,Lee, Dae Won,Shim, Yoon Bo 한국분석과학회 2001 분석과학 Vol.14 No.1

환경 시료인 토양, 우유, 해수, 해조류, 어패류, 솔잎 및 해저토 중에 존재하는 $^{90}Sr$의 농도를 결정하기 위한 새로운 형태의 분리농축법을 확립한 후 ICP-AES를 사용하여 각 분석과정을 검토하였다. 처리 과정을 단축시킨 발연 질산법 및 이온교환수지법을 혼합한 새로운 형태의 분석과정으로 $^{90}Sr$의 농도를 정량한 결과 효과적으로 Ca를 완전히 제거할 수 있었으며, 발연질산법 만을 사용했을 경우보다 스트론튬의 회수율이 약 10% 이상 높게 나타났다. 이 방법은 토양, 우유, 해조류, 어패류, 솔잎, 및 해저토 등 환경 시료들 중의 $^{90}Sr$ 결정에 적용할 수 있음을 확인하였다. 분석 결과, 토양 중의 $^{90}Sr$ 농도가 가장 높았으며 모든 시료 중의 $^{90}Sr$의 농도는 환경 기준치 이하로 나타났다. New process to determine $^{90}Sr$ in the environmental samples was established by investigating the existing methods. The environmental samples included soil, milk, seaweed, fishes and clams, pine needles, and marine sediment. Using the developed method combined with fuming nitric acid and ion exchange resin, which could be reduced the treatment step, we could be removed Ca effectively for the determination of $^{90}Sr$. The recovery yield of $^{90}Sr$ in this method was 10% higher than those of using the fuming nitric acid only. This method could be applied to all environmental samples we choose. The content of $^{90}Sr$ in soil was the highest value in the tested environmental samples.

N'-phenyl-N-(2-chloroethyl)-N-nitrosourea 유사체의 전기화학적 거동 및 N-aminourea의 합성

원미숙,김정균,심윤보,Won, Mi-Sook,Kim, Jeong-Gyun,Sim, Yoon-Bo 대한화학회 1991 대한화학회지 Vol.35 No.6

수은 pool 전해조를 사용하여 (5:3) EtOH/4N-HCl 용액계에서 선택적인 -N-N=O기의 전기화학적 환원에 의해 N'-phenyl-N-(2-chloroethyl)-N-nitrosourea로부터 N'-phenyl-N-(2-chloroethyl)-N-aminourea를 합성하였다. 전기화학적 환원에 앞서 반응의 최적조건을 검토하기 위하여 몇 가지 N'-aryl-N-(2-chloroethyl)-N-nitrosourea 유사체에 대하여 pH변화에 따른 환원전위를 순환전압전류법으로 조사한 결과 pH에 따라 $E_p$값이 "-"쪽으로 이동하였으며 aryl기의 치환기 효과는 -N-N=O기의 환원 전위에 크게 영향을 미치지 않는 것으로 나타났다. 용액계의 pH에 따른 반응성을 조사한 결과 강산성 용액(pH<1)에서 반응이 가장 잘 진행되며 -0.7 V vs. Ag/AgCl/4 N-HCl에서 N-N=O기의 선택적인 4전자 환원반응에 의해 N-N$H_2$가 생성됨을 확인하였다. N'-phenyl-N-(2-chloroethyl)-N-aminourea has been prepared from N'-phenyl-N-(2-chloroethyl)-N-nitrosourea by means of the electrochemical reduction with the mercury pool electrolytic cell. In order to find out the optimum condition of the reaction, the voltammetric behaviors for N'-aryl-N-(2-chloroethyl)-N-nitrosourea derivatives have been investigated by the cyclic voltammetry and polarography. The peak potentials was shifted to the negative direction as the pH value of the solution decrease. The substituent effects of phenyl ring on the peak potential were not observed in this case. (5:3) EtOH/4 N-HCl mixed solution was employed for the electrolysis. The applied potential was -0.7 V vs. Ag/AgCl/4 N-HCl electrode. The number of electrons participated to the reduction process was 4, respectively. The product was identified by FT-IR, NMR, mass and/or elemental analysis data.

N'-aryl-N-alkyl-N-nitrosourea 유도체의 환원반응에 대한 전기화학적 거동

원미숙,김정균,정의덕,심윤보,Won, Mi Sook,Kim, Jack C.,Jeong, Euh Duck,Shim, Yoon-Bo 대한화학회 1995 대한화학회지 Vol.39 No.11

유리질 탄소전극을 사용하여 조사한 몇가지 N'-aryl-N-alkyl-N-nitrosourea 유도체들의 전기화학적 환원반응은 확산지배적이고 비가역적인 반응이었다. 이들 유도체들의 환원 반응시의 교환속도 상수 $k_0$값은 $1.48{\times}10^{-6}{\sim}5.32{\times}10^{-7}\;cm/sec.$의 값을 나타내었었다. Aryl기 및 alkyl기의 치환기에 따른 교환속도상수는 $k_0$ N'-aryl-N-alkyl-N-nitrosourea 에서 aryl기가 phenyl일 경우, 다른 치환기보다 $k_0$값이 1.3-2.8배였다. N'-aryl-N-alkyl-N-nitrosourea 와 N'-aryl-N-(2-chloroethyl)-N-nitrosourea의 두 화합물에서 aryl기의 치환기가 같을 경우는 비슷한 값을 나타내었다. N'-aryl-N-methyl-N-nitrosourea 유도체는 pH값이 높아짐에 따라 $E_p$값이 음전위쪽으로 이동하며 각 환원 반응에 참여한 $H^+$의 수는 4-5개였다. 이경우 aryl기의 치환기 효과는 환원전위에 크게 영향을 미치지 않는 것으로 나타났다. The electrochemical reduction reactions of N '-aryl-N-alkyl-N-nitrosourea derivatives with a glassy carbon electrode were diffusion controlled and irreversible. The exchange kinetic constant ko values for reduction reaction of the N '-aryl-N-alkyl-N-nitrosoureas were at the range of $1.48{\times}10^{-6}{\sim}5.32{\times}10^{-7}\;cm/sec.$ The $k_0$ values for phenyl substituted on the aryl position were about 1.3∼2.8 times higher than that of other substituents. The same substituent for aryl groups on the both of N '-aryl-N-alkyl-N-nitrosourea and N '-aryl-N-(2-chloroethyl)-N-nitrosourea exhibited same value. The $E_p$ value was shifted to the negative direction as pH increased. The number of protons participated to the reduction was 4∼5, respectively. The substituent effect of aryl group on the reduction potential was not observed in this case.

치주 골내낭에서 anorganic bovine-derived hydroxyapatite matrix(ABM)/cell binding peptide(P-15)의 임상적효과

원미숙,백정원,김창성,조규성,채중규,김종관,최성호,Won, Mi-Sook,Paik, Jeong-Won,Kim, Chang-Sung,Cho, Kyoo-Sung,Chai, Jung-Kiu,Kim, Chong-Kwan,Choi, Seong-Ho 대한치주과학회 2002 Journal of Periodontal & Implant Science Vol.32 No.3

The ultimate goal of periodontal therapy is the regeneration of periodontal tissue which has been lost due to destructive periodontal disease. To achieve periodontal regeneration, various kinds of methods have been investigated and developed, including guided tissue regeneration and bone graft. Bone graft can be catagorized into autografts, allografts, xenografts, bone substitutes. And materials of all types have different biological activity and the capacity for periodontal regeneration, but ideal graft material has not been developed that fits all the requirement of ideal bone graft material. Intensive research is underway to identity, purify, synthesize a variety biologic modulators that may enhance wound healing and regeneration of lost tissues in periodontal therapy. The present study evaluates the effects of ABM/P-15 on the periodontal regeneration in intrabony defects of human. We used thirty four 2-wall or 3-wall osseous defects in premolars and molars of chronic peridontitis patient that have more than 5mm pockets and more than 3mm in intrabony defect. 12 negative control group underwent flap procedure only, 11 positive control group received DFDBA graft with flap procedure, and 11 experimental group received ABM/P-15 graft with flap procedure. The changes of probing pocket depth, loss of attachment and bone probing depth following 6months after treatment revealed the following results: 1. The changes of probing pocket depth showed a statistically significant decrease between after scaling and 6months after treatment in negative control(2.0${\pm}$0.9mm), positive control(3.0${\pm}$0.9mm), and experimental group (3.4${\pm}$1.5mm) (P<0.01). Significantly more reduction was seen in experimental group compared to negative control group (P<0.05). 2. The changes of loss of attachment showed a statistically significant decrease between after scaling and 6months after treatment in positive control(2.0${\pm}$0.6mm), and experimental group (2.2${\pm}$l.0mm) except negative control group(0.1${\pm}$0.7mm) (P<0.01). Significantly more reduction was seen in both experimental and positive control group compared to negative control group(P<0.05). 3. The changes of bone probing depth showed a statistically significant decrease between after scaling and 6months after treatment in positive control(2.7${\pm}$l.0mm), and experimental group (3.4${\pm}$1.3mm) except negative control(0.l${\pm}$0.9mm) (9<0.01). Significantly more reduction was seen in both experimental and positive control group compared to negative control group (P<0.05). The results suggest that the use of ABM/P-15 in the treatment of periodontal intrabony defects can reduce loss of attachment and bone probing depth more than flap operation only. It suggests that ABM/P-15 may be an effective bone graft material for the regeneration of periodontal tissue in intrabony defects.

수은막 GCRRDE를 이용한 해수 중 중금속의 동시 종분리 정량

원미숙 ( Mi Sook Won ),최만식 ( Man Sik Choi ),윤장희 ( Jang Hee Yoon ) 한국환경분석학회 2004 환경분석과 독성보건 Vol.7 No.3

환경시료 중 ^(90)Sr의 분석을 위한 분리농축법 연구

원미숙(Mi Sook Won),조규봉(Kyu Bong Cho),윤장희(Jang Hee Yoon),이대원(Dae Won Lee),심윤보(Yoon Bo Shim) 한국분석과학회 2001 분석과학 Vol.14 No.1

L-Sparteine 수식전극을 사용한 Hg (II) 이온의 정량

정의덕,원미숙,심윤보,Euh Duck Jeong,Mi-Sook Won,Yoon-Bo Shim 대한화학회 1991 대한화학회지 Vol.35 No.5

A mercury ion-sensitive carbon-paste electrode (CPE) was constructed with l-sparteine. Mercury (II) ion was chemically deposited by the complexation with l-sparteine onto the CPE. The surface of CPEs was characterized by cyclic voltammetry and anodic stripping voltammetry in an acetate buffer solution, separately. Exposure of CPEs to acid solution could regenerate surface and reuse it for deposition. In 5 deposition/measurement/regeneration cycle, the response was reproducible and in licnear up to $2.0\;{\times}\;10^{-6}$ M with linear sweep voltammetry. In case of using the differential pulse technique, we have obtained the linear response up to $7.0 {\times}10^{-7}$ M with relative standard deviation of ${\pm}5.1$%. The detection limit was $5.0{\times}10^{-7}$ M for 20 minutes of the deposition. We have investigated the interference effect of various metal ions, which are expected to form the complex with ligand. Silver (I) ion of these has interfered with the analysis of Hg (II) ions. However, pretreatment of the silver (I) ion with potassium chloride led to no interference on the analysis of mercury ions in aqueous solution. 수은 이온의 정량을 위해 l-sparteine으로 처리한 carbon paste 전극(CPE)을 제작하였다. 수용액중의 수은 이온을 CPE에 수식 시킨 l-sparteine과 반응시켜 착화합물 상태로 전극표면에 석출시킨 후 이 착물을 벗김 전압-전류곡선법을 사용하여 정량하였다. 또한 수식된 CPE의 전극반응을 순환 전압 전류 곡선법을 사용하여 초산/초산염 완충용액에서 조사하였다. 한번 사용한 전극은 산 용액으로 처리하여 5회 이상 재사용할 수 있었다. 선형주사 전압전류법을 사용하였을 경우 $2.0{\times}10^{-6}$ M 농도까지 정량이 가능하였다. 시차펄스 전압전류법으로 실험할 경우 $7.0 {\times}10^{-7}$ M 농도까지 직선적으로 감응함을 알 수 있었으며 그 때의 상대 표준편차는 ${\pm}$5.1% 이었다. 이 경우 검출 한계는 $5.O{\times} 10^{-7}$ M이었다. 리간드와 착물을 형성할 것으로 예상되는 여러 금속이온에 대한 방해 작용을 조사하였다. 그 결과 은(I) 이온이 방해를 하지만 염화칼륨으로 사전 처리하여 방해작용을 제거할 수 있었다.

${\iota}$ -Sparteine으로 변성된 Carbon Paste 전극을 사용한 Co(II) 이온의 펄스 차이 전압-전류법 정량

정의덕,원미숙,박덕수,심윤보,최성낙,Eu-Duck Jeong,Mi-Sook Won,Deog-Su Park,Yoon-Bo Shim,Sung-Nak Choi 대한화학회 1993 대한화학회지 Vol.37 No.10

코발트(II) 이온의 정량을 위해 ${\iota}$-sparteine(SP)으로 변성된 탄소 반죽 전극(carbon paste electrode : CPE)을 제작하였다. 수용액 중의 코발트 이온을 CPE에 처리시킨${\iota}$-sparteine과 반응시켜서 착화합물 상태로 전극표면에 석출시킨 후, 이 착물을 전압-전류법을 사용하여 정량하였다. 또한 변성된 CPE의 전극반응을 순환 전압-전류법을 사용하여 아세트산/아세트산염 완충용액에서 조사하였다. 한번 사용한 전극은 산용액으로 처리하여 5회 이상 재사용할 수 있었다. CPE에 흡착된 Co(II)-SP 착물의 산화환원 전위는 0.17V / 0.27V였으며 산화피이크는 0.17V의 환원피이크를 지나야 나타나는 피크이다. 이는 전극표면의 SP와 용액내의 Co(II)이온이 착물을 형성하여 흡착되고, 이 전극표면에 형성된 착물이 산화환원되어 나타나는 피이크이다. 펄스차이 전압-전류법을 사용할 경우 $2{\times}10^{-7}$M 농도까지 직선적으로 감응함을 알 수 있었으며, 그 때의 상대표준 편차는 ${\pm}$5.6%이었다. 이 경우 검출한계는 $1.0{\times}10^{-7}$M이었다. 리간드와 착물을 형성할 것으로 예상되는 여러 금속이온에 대한 방해작용을 조사하였다. A cobalt(II) ion-selective carbon-paste electrode (CPE) was constructed with ${\iota}$-sparteine. Cobalt(II) ion in aqueous solution was chemically deposited through the complexation with ${\iota}$-sparteine onto the CPE. The surface of CPEs were characterized by cyclic voltammetry and differential pulse voltammetry in an acetate buffer solution, separately. Exposure of the CPEs to an acid solution could regenerate surface to reuse it for the deposition. In more than 5 deposition / measurement / regeneration cycles, the response was reproducible and linear up to $5.0{\times}10^{-6}$M with linear sweep voltammetry. The peaks at 0.17V / 0.27V were correspond to the redox of Co(II)-SP complex deposited on CPE. The anodic peak of which appeared after scan over the cathodic peak of 0.17 V to more negative scan. In case of using the differencial pulse voltammetry (DPV), we have obtained the linear response $2.0{\times}10^{-7}$M with relative standard deviation ${\pm}5.6%$. The detection limit was $1.0{times}10^{-7}$M for 20 minutes of the deposition. We have also investigated the interference effect of various metal ions, which are expected to form the complex with the ligand on the electrode.

산화전위수 양치용액 사용이 만성 치주질환에 미치는 효과에 대한 연구

조규성,원미숙,정현철,정정학,최성호,채중규,김종관,Cho, Kyoo-Sung,Won, Mi-Sook,Chul, Hyun-Chul,Jung, Jung-Hwak,Choi, Seong-Ho,Chai, Jung-Kui,Kim, Chong-Kwan 대한치주과학회 1997 Journal of Periodontal & Implant Science Vol.27 No.4

The purpose of this study was to assess the antimicrobial and clinical effects of acid water mouthrinse prepared by an electrolysis apparatus on chronic periodontitis and to evaluate the lasting period of these effects. The change' in the pattern of colonization of bacteria within the subgingival pockets was monitored by phase contrast microscopy, in 40 patients, over a period of 8 weeks. In addition, changes in the clinical parameters of the diseased sites were also monitored. Site of pocket ${\geq}$ 5mm was selected in each patient randomly divided into two groups. As a test group, acid water mouthrinse was used twice a day in 20 patients. As control, no mouthrinse was used in 20 patients The results were as follows : 1. The suppression of motile bacteria was maintained for up to 3-4 weeks at test group. 2. Two groups did not differ significantly in proportion of bacteria in subgingival plaque over a period of 8 weeks. 3. Loss of attachment showed a significant difference in test group and in test group compared with control group (P<0.05), but there was no significant difference in control group. 4. No statistical difference was shown in two groups concerning the gingival index, plaque index, bleeding index. The results suggest that acid water mouthrinse is effective for reducing subgingival bacteria. It can be concluded that acid water may be useful as an mouthrinsing agent.

One-Chip 마이크로컴퓨터를 사용한 휴대용 페놀 감지 시스템의 개발

심윤보,김경희,원미숙,박현,진창수,Sim, Yoon Bo,Kim, Kyung Hee,Won, Mi Sook,Park, Hyun,Jin, Chang Soo 대한화학회 1996 대한화학회지 Vol.40 No.5