Hamster 수정란(受精卵)의 동결보존(凍結保存) 및 생존성(生存性)에 관한 연구(硏究)

오원진,이규승,김영묵,Oh, Won Jin,Lee, Kyu Seung,Kim, Young Mook 충남대학교 농업과학연구소 1986 Korean Journal of Agricultural Science Vol.13 No.1

본(本) 실험(實驗)은 hamster 수정란(受精卵)의 동결보존시(凍結保存時) 가장 적합(適合)한 동결(凍結)및 융해온도(融解溫度)를 구명(究明)하기 위(爲)하여 실시(實施)하였다. 수정란(受精卵)은 hamster 두당(頭當) 30 IU의 PMSG를 복탈내(腹脫內)에 투여(投與)하고 4일후(日後)에 교미(交尾)를 시켰으며 교미(交尾) 3일후(日後)에 도살(屠殺)하여 자궁(子宮)과 난관(卵管)을 관류(灌流)하여 회수(回收)하였다. 회수(回收)된 수정란(受精卵)의 동결(凍結)에 사용(使用)한 보존액(保存液)은 Dulbecco's phosphate buffered saline(PBS)이었으며, 동해방지제(凍害防止劑)로는 Dimetyl sulfoxide (DMSO)로서 3단계(段階)로 최종농도(最終濃度)가 1.5M이 되게 하였다. 수정란(受精卵)의 동결방법(凍結方法)은 실온(室溫)에서 $-6^{\circ}C$까지 $1^{\circ}C/min$의 속도(速度)로 하강(下降)시켜 수직(水植)하고 3 분간(分間) 방치(放置)시킨 다음 $0.30^{\circ}C/min$ 속도(速度)로 $-35^{\circ}C$까지 냉각(冷却)시켰으며, 다음에 $-70^{\circ}C$까지는 $0.1^{\circ}C/min$, $1^{\circ}C/min$ 및 $10^{\circ}C/min$의 3 처리(處理)로 냉각(冷却)시킨후 $-196^{\circ}C$의 액체질소(液體窒素)에 보존(保存)하였다. 융해(融解)는 $4^{\circ}C/min$과 $12^{\circ}C/min$의 속도(速度)로 $37^{\circ}C$ 까지 가온(加溫)하는 방법(方法)과 $37^{\circ}C$의 water bath에서 2 분간(分間) 침지(沈漬)시키는 방법(方法)을 이용(利用)하였다. 평균배란점(平均排卵點)은 35.1개(個)인데 대하여 회수(回收)된 수정란(受精卵)은 27.0개(個)로서 회수율(回收率) 77.0% 였으며, 수정란(受精卵)중에서 8세포기(細胞期) 배(胚) 24.8개(個)로서 그 비율(比率)은 70.6%였다. 수정란(受精卵)의 동결속도(凍結速度)에 따른 생존율(生存率)은 $0.1^{\circ}C/min$으로 동결(凍結)했을 때 55.5~67.7%, $1^{\circ}C/min$에서는 58.8~64.9%, $10^{\circ}C/min$에서는 40.5~44.7%였는데 $10^{\circ}C/min$의 속도(速度)로 동결(凍結)한 것이 유의(有意)하게 나쁜 성적(成績)이었다. 융해방법(融解方法)에 따른 수정란(受精卵)의 평균생존율(平均生存率)은 $4^{\circ}C/min$에서 53.5%, $12^{\circ}C/min$에서 53.7%, $37^{\circ}C$ water bath 융해(融解)에서 59.1%를 나타내어 water bath 융해(融解)가 가장 좋은 성적(成績)이었으나 큰 차이(差異)는 인정(認定)되지 않았다. 본(本) 실험(實驗)의 결과(結果)를 종합(綜合)해 볼 때 hamster 난자(卵子)의 동결(凍結)에는 실온(室溫)에서 $-6^{\circ}C$까지는 $1^{\circ}C/min$, $-35^{\circ}C$까지 $0.3^{\circ}C/min$, $-70^{\circ}C$까지 $0.1^{\circ}C/min$ 또는 $1^{\circ}C/min$의 동결속도(凍結速度)를 유지(維持)하고, $37^{\circ}C$의 water bath에서 2 분간(分間) 침관(沈漬)하여 융해(融解)하는 것이 가장 높은 생존율(生存率)을 얻었다. This experiment was carried out to determine the optimum freezing and thawing rates of the hamster embryos. The female hamsters were induced to superovulate by intraperitoneal injections of 30 i.u. PMSG and mated with males of the same strain of 4 days the PMSG injection. They were killed and embryos were flushed from the oviduct and uterine horn on 3 days after mating. Embryos were flushed with a modified Dulbecco's phosphate-buffered saline and equilibrated with 1.5 M-dimethylsulphoxide by a 3-step procedure. The freezing rates of the samples were $1^{\circ}C/min$ from room temperature to $-6^{\circ}C$ and the samples were seeded at $-6^{\circ}C$. After being held for 3 min at the seeding temperature, the rates were $0.3^{\circ}C/min$ from $-6^{\circ}C$ to $-35^{\circ}C$. From $-35^{\circ}C$ to $-70^{\circ}C$, the rates were divided into $0.1^{\circ}C/min$, $1^{\circ}C/min$ and $10^{\circ}C/min$, respectively. At $-70^{\circ}C$ the samples were plunged directly into liquid nitrogen. The samples were thawed at $4^{\circ}C/min$ and $12^{\circ}C/min$ from $-196^{\circ}C$ to $37^{\circ}C$, and for 2 min in $37^{\circ}C$ water bath, respectively. The average numbers of ovulation points and embryos recovered were 35.1 and 27.0 appearing 77.0% recovery rates. Eight cell embryos in the embryos recovered were 24.8. The survival rates of embryos according to the freezing rates were 55.5~67.7% at $0.1^{\circ}C/min$, 58.8~64.9% at $1^{\circ}C/min$ and 40.5~44.7% at $10^{\circ}C/min$, respectively. The survival rates at $10^{\circ}C/min$ were significantly low. The survival rates of embryos according to the thawing rates were 53.5% at $4^{\circ}C/min$, 53.7% at $12^{\circ}C/min$ and 59.1% in $37^{\circ}C$ water bath. The survival rates, in $37^{\circ}C$ water bath were slightly higher, but we did not find any differences among them. In conclusion, the best freezing rates of hamster embryos were $1^{\circ}C/min$ from the room temperature to $-6^{\circ}C/min$, $0.3^{\circ}C/min$ from $-6^{\circ}C/min$ to $-35^{\circ}C$ and $-0.1^{\circ}C/min$ or $1^{\circ}C/min$ from $-35^{\circ}C$ to $-70^{\circ}C$. The hamster embryos thawed for 2 min in $37^{\circ}C$ water bath showed the best survival rates.

영역기반정합에 의한 수치영상의 해상도 강화에 관한 연구

오원진(Oh, Won-Jin),배연성(Bae, Yeon-Soung),주영은(Joo, Young-Eun) 한국측량학회 2000 한국측량학회지 Vol.18 No.3

현미경에 의한 초근접 수치사진측량

오원진(Oh Won-Jin),손세원(Shon Sae-Won),배연성(Bae Yeon-Soung) 한국지적정보학회 2000 한국지적정보학회지 Vol.2 No.-

최근 각광을 받고 있는 수치사진측량분야는 하드웨어와 소프트웨어의 발달에 힘입어 그 활용 영역이 광범위하게 확대되고 있다. 특히, 신소재나 치 의학과 같은 연구 분야에서는 현미경을 통한 정량적인 3차원 해석이 매우 유용한 수단으로 제시될 수 있어 수치사진측량기법의 도입은 매우 중요한 의미를 지닌다. 현미경 사진측량의 경우 사진기의 위치가 고정되어 있고, 시야 각이 좁아 적당한 기선거리비로 입체시를 얻을 수 없을 뿐만 아니라, 대상물이 작아 기준점을 부착하고 3차원 좌표를 관측할 수 있는 공간을 확보하는데 제약이 따른다. 또한 광섬유를 조명으로 사용하므로 특별한 주의가 요망된다. 이와 같이 비정상적인 촬영 기하학으로 인하여 기준점 성과의 획득은 물론 결과의 객관적인 평가에도 어려움이 있어 이들 난제를 해결하기 위한 연구가 선행되어야 한다. 본 연구에서는 입체시를 얻기 위하여 대상물을 기울여 촬영하고, 기준점 좌표의 획득, 결과의 독립적인 평가 및 영상 기하학의 검증을 위하여 ADAM MPS 해석도화기를 사용하였으며, 수치사진측량 시스템인 VirtuoZo를 이용하여 해석함으로써 등고선도, 정사사진 및 DTM을 효율적으로 생성할 수 있어 추후 이에 대한 폭넓은 활용이 기대된다. As for the digital Photogrammetry which is being highlighted recently, the utilization domain is being expanded extensively thanks to the development of the computer hardware and software. Especially, in the study fields such as material sciences or dentistry, the introduction of digital photogrammetry has very important meaning, as the quantitative 3-dimensional analysis through microscope may be suggested as very useful means. In case of digital photogrammetry, it is impossible to obtain stereoscopic with proper ratios of base-to-object-distance, as the position of camera is being fixed and the field of view is narrow. Besides, as the object is small, restriction follows in securing the space to be able to attach control point and observe 3-dimensional coordinates. In addition, special attention is demanded, fiber optic is used as light. As three is the difficulty in the objective evaluation of result as well as the acquisition of result of control point due to the photographic geometry to be abnormal like this, the study for solving these difficult problems should precede. In this study, this researcher photographed by leaning object for obtaining stereoscopic and used ADAM MPS analytical plotter for the acquisition of coordinates of control point, the independent evaluation of result and the verification of imaging geometry. And, as analysis by using VirtuoZo which is digital photogrammetry system may generate contour map, ortho image and DTM efficiently, the wide utilization about this is expected afterwards.

Hamster 受精卵의 凍結保存 및 生存性에 관한 研究

吳元鎭(Won Jin Oh),李揆丞(Kyu Seung Lee),金榮默(Young Mook Kim) 충남대학교 농업과학연구소 1986 農業技術硏究報告 Vol.13 No.1

z현미경 수치사진측량의 효용성에 관한 연구

오원진(Won-Jin Oh) 한국지적정보학회 1999 한국지적정보학회지 Vol.1 No.-

Multiple Method에 의한 Strip 조합해석(組合解析)에 관한 연구(硏究)

강준묵,오원진,한승희,Kang, Joon Mook,Oh, Won Jin,Han, Seung Hee 대한토목학회 1989 대한토목학회논문집 Vol.9 No.3

대부분의 구조물(構造物)들은 전면해석(全面解析)을 요(要)하는 경우가 많으므로 이에 대한 정확(正確)하고도 이상적(理想的)인 해석기법(解析技法)의 개발(開發)이 요망(要望)되며, 정밀측정(精密測定)을 위(爲)하여 비측정용(非測定用) 카메라를 사용(使用)하거나 비측정용(非測定用) 카메라와 측정용(測定用) 카메라를 조합(組合)하여 사용(使用) 할 수 있다면 경제적(經濟的)이고 결속(決速)하게 대상물(對象物)을 정밀측정(精密測定) 할 수 있을 것이다. 본(本) 연구(硏究)에서는 전면해석(全面解析)을 요(要)하는 원형(圓形) 구조물(構造物)을 대상(對象)으로 수렴(收斂) 다중사진측량(多重寫眞測量)을 실시(實施), bundle 조정법(調整法)에 의한 원형(圓形) 스트립해석(解析)의 가용성(可龍性)을 제시(提示)하고, 회전요소(回轉要素) ${\omega}$, ${\varphi}$를 변화(變化)하여 촬영(撮影)한 tm트립을 동시(同時) 해석(解析)함으로써 정확도(正確度)의 향상(向上)을 꾀함은 물론 측정용(測定用) 카메라와 비측정용(非測定用) 카메라에 의한 스트립의 조합해석(組合解析)을 실시(實施)하여 이의 적용(適用) 타당성(妥當性)을 제시(提示)하는데 그 목적(目的)이 있다. As most structures need all sided analysis, development of precise and ideal analytical techniques are required. In non-metric camera alone or metric and non-metric camera can be used for the purpose of measurement, the object will be analyzied economically and quickely. The purposes of this study are to suggest possibility of analysis of circular strip by means of Bundle Adjustment and to investigate efficiency of analytical method by combination of metric and non-metric camera. For these purposes, cylindrical structure was taken as the object and multiple convergent photographs changing ${\omega}$ and ${\phi}$, by which circular strip was formed, were analysed.

비측량용(非測量用) 사진기(寫眞機)에 의(依)한 근접사진(近接寫眞) 해석(解析)의 정확도(正確度) 향상(向上)에 관(關)한 연구(?究)

강준묵,오원진,한승희,Kang, Joon Mook,Oh, Won Jin,Han, Seung Hee 대한토목학회 1992 대한토목학회논문집 Vol.12 No.2

근거리 사진측량에서 정오차의 보정은 정확도 향상에 중요한 영향을 미치며, 특히 비측량용사진기를 정밀측량에 이용하기 위해서는 정오차에 대한 보정이 선행되어야 한다. 이를 위해 비측량용사진기에 지표를 설치하고 collimator검정방법에 의해 렌즈의 PPS, PPA, EFL, CFL 및 각 상한별 렌즈의 방사방향 왜곡계수를 구하였고, plumb line 검정방법에 의해서도 렌즈의 방사방향 및 접선방향에 대한 왜곡계수를 구하여 정오차 보정을 효율적으로 시도하였다. 이 검정된 비측량용사진기를 이용하여 근접사진측량을 실시하고, 정오차를 보정하므로써 정확도 향상을 꾀할 수 있었다. In close-range photogrammetry, to employ non-metric camera especially for the purpose of precise measurement, systematic errors must, first of all, be corrected as they have a great influence on accuracies of results. For it, fiducial marks was built in non-metric camera and the factors such as PPS, PPA, EFL, CFL, and radial lens distortion coefficients for each quadrant were calibrated through collimator test. Also, the coefficients of both radial and tangential lens distortion were calibrated from analytical plumb line method and therefore main systematic errors could be effectively corrected. Using the calibrated non-metric camera, close-range photogrammetry could be successfully accomplished and accuracies could be improved sharply.

MDPS 로직 파라미터에 따른 스티어링 성능 영향도의 실험적 연구

이동원(Dong Won Lee),한상호(Sang Ho Han),천세원(Se Won Cheon),오원진(Won Jin Oh) 한국자동차공학회 2011 한국자동차공학회 부문종합 학술대회 Vol.2011 No.5

Motor Driven Power Steering which is broadly used in vehicle industry has a lot of advantages and tuning potential. To define the effect of tuning parameters on steering performance, subjective and objective evaluations were performed. As a result, the relationship and the tuning direction of each parameter were obtained. In addition, desirable steering performance was discussed with reference vehicles. With the result of parameter study, the logic tuning was performed and the steering performance of tuning vehicle was successfully closed to target vehicles. A further study will be needed to enhance the relationship between steering performance and mechanical characteristics of MDPS system.

해체 디지털목업시스템 아키텍쳐 구현

박희성,김성균,이근우,오원진,Park Hee-Seoung,Kim Sung-Kyun,Lee Kune-Woo,Oh Won-Jin 한국방사성폐기물학회 2005 방사성폐기물학회지 Vol.3 No.3

It is necessary to forecast the various dismantling activities prior to dismantling nuclear facilities by using various software instead of a physical mock-up system because the dismantling in a contaminated with radioactivity cause the results of an unexpected situation. The component that needs to develop a dismantling mock-up system was examined. There are many component systems such as a decommissioning database system,3D dosimetric mapping that represents a distribution of a radionuclide contamination, a component of modeling for nuclear facility and devices include the decontamination and decommissioning. The research of software architecture about these components was carried out because these component systems that have been independently doesn't describe not only to visual an activities of Decontamination and Decommissioning(D&D) but also to evaluate it. The result was established an architecture that consist of an visualization module which could be visualized an D&D activities and a simulation module which can be evaluated a dismantling schedule and decommissioning cost.

PFC제염폐액 내의 미립자 제거를 위한 여과막의 특성 연구

김계남,이성열,원휘준,정종헌,오원진,박진호,Kim Gye-Nam,Lee Sung-Yeol,Won Hui-Jun,Jung Chong-Hun,Oh Won-Zin,Park Jin-Ho 한국방사성폐기물학회 2005 방사성폐기물학회지 Vol.3 No.2

PFC(Perfluorocarbon) decontamination process is one of best methods to remove hot particulate adhered at inside surface of hot cell and surface of equipment in hot cell. It was necessary to develop a particulate filtration equipment to reuse PFC solution used on PFC decontamination due to its high cost and to minimize the volume of second wastewater. Contamination characteristics of hot particulate were investigated and then a filtration process was presented to remove hot particulate in PFC solution generated through PFC decontamination process. The removal efficiency of PVDF(Poly vinylidene fluoride), PP(Polypropylene), Ceramic(Al$_{2}$O$_{3}$ filter showed more than 95$\%$. The removal efficiency of PVDF filter was a little lower than those of other kiters at same pressure(3psi). A ceramic filter showed a higher removal efficiency with other filters, while a little lower flux rate than other filters. Due to inorganic composition, a ceramic filter was highly stable against radio nuclides in comparison with PVDF and PP membrane, which generate H$_{2}$ gas in e-radioactivity atmosphere. Therefore, the adoption of ceramic filter is estimated to be suitable for the real nitration process.