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배양액의 에너지원 조성이 생쥐 초기배 발달에 미치는 효과에 관한 연구
이종범(Jong Bum Lee),김주환(Ju Hwan Kim),고지환(Jee Hwan Ko),오영균(Young Kun Oh),손성경(Song Kyong Son),서영석(Young Seok Seo),노흥태(Heung Tae Noh),강길전(Kil Chun Kang),송해범(Hai Bum Song),이기환(Ki Hwan Lee) 대한산부인과학회 2002 Obstetrics & Gynecology Science Vol.45 No.3
N/A Objective: The objective of this study was to examine the effect on development of mouse preimplantation embryos in culture media with different composition of energy sources in vitro culture. Methods: Two hundred and seventy one two-cell embryos were cultured in four different culture system for 96 hours. Group I (n=61)was cultured in DMEM-G (DMEM with glutamine) only, group II (n=64)was cultured in DMEM-GGP (DMEM with glutamine, glucose and pyruvate) only, group III(n=72) was cultured for 48hoursinDMEM-G and then transferred to DMEM-GGP and group IV (n=74)was cultured for 48 hours in DMEM-GGP and then transferred to DMEM-G. Development of embryos in each group was observed every 24 hours. Results: After 24 hours, the rate of development ≥3-cell was significantly higher in group II(87.5%) and IV(86.5%)compared with group I(59.0%)and III(62.5%).After 48 hours, the rate of development into ≥morula stage was significantly higher in Group II(79.7%)and IV(86.5%)compared with group I(34.4%) and III(37.5%).After 72 hours, the rate of development into blastocyst was significantly higher in group IV(74.3%)compared with group I (49.2%)and III (45.8%).After 96 hours, the rate of development into ≥expanded blastocyst was significantly higher in group IV(70.3%)compared with group I (32.8%), II ( 53.1%), and group III (40.3%) Conclusion: Mouse preimplantation embryos development was the most effective in culture system with DMEM-GGP for 48 hours and then transferred to DMEM-G.
고지환(Jee Hwan Ko),이종범(Jong Bum Lee),오영균(Young Kun Oh),손성경(Song Kyong Son),서영석(Young Seok Seo),이기환(Ki Hwan Lee),남상륜(Sang Lyun Nam),노흥태(Heung Tae Noh),강길전(Kil Chun Kang) 대한산부인과학회 2001 Obstetrics & Gynecology Science Vol.44 No.8
N/A Objective : The study was designed to find out current state of conservative treatment modalities for cervical pregnancies in korean. Method : 64 cases of cervical pregnancies reported by the joining of The Korean Society of Obstetrics & Gynecology from 1960 to 2000 and the 10 cases at Chungnam National University Hospital were analyzed retrospectively. Results : The average age of the 74 cases was 31.7±6.6 years, and the average gestational age at the time of diagnosis was 8.3±3.4 weeks. The average duration of hospitalization was 8.2±4.8 days. Most (67/74) of the patients had previously experienced delivery or abortion. Out of the 74 cases, 36 had total hysterectomy; 4 cases had curettage; 12 were used only methotrexate; 11 had combined curettage and methotrexate; 10 cases had Foley catheter ballooning after curettage. From the 1960's to the end of 1980's, 33 (84.6%) cases out of 39 were performed hysterectomy. Whereas mainly conservative methods, in particular methotrexate treatment, were performed in the 1990's. Among the conservative modalities, Foley catheter ballooning after curettage had the shortest length of hospitalization with 4.3 days. Conclusion : After comparing the conservative methods for treating cervical pregnancies, it was found that methotrexate treatment was performed the most; however, Foley catheter ballooning after curettage seems to be an excellent treatment option.