형질전환 감자 소괴경의 발달단계에 따른 Patatin Promoter-GUS 유전자의 발현 분석

염정원,김미선,강원진,정혁,김현순,전재흥,이병찬 한국식물생명공학회 2003 JOURNAL OF PLANT BIOTECHNOLOGY Vol.30 No.1

Effects of the Enhanced Recovery After Surgery (ERAS) Program for Colorectal Cancer Patients Undergoing Laparoscopic Surgery

염정원,임희숙 한국임상영양학회 2022 Clinical Nutrition Research Vol.11 No.2

This study sought to investigate the effects of the enhanced recovery after surgery (ERAS) program on postoperative recovery and nutritional status in patients with colorectal cancer undergoing laparoscopic surgery. A total of 37 patients were included: 19 in the experimental group and 18 in the control group. The experimental group was supplemented with carbohydrate drinks before and after surgery, and the control group was maintained with fasting and water intake in the traditional method. Both care management and nutrition education were implemented for both groups. Patients were evaluated for physical condition, clinical indicators, blood tests, pain, length of stay, nutritional status, and nutrient intake. Use of the ERAS program for the experimental group resulted in shorter length of stay (p = 0.006), less pain (p < 0.001), and a lower rate of malnutrition (p = 0.014) compared with controls. In conclusion, carbohydrate drinks provide great advantages by reducing discomfort, such as pain or thirst, during fasting in patients after colon cancer surgery, helping patients to eat comfortably and actively, minimizing insulin resistance, maintaining nitrogen balance, and reducing infection and anastomosis leakage. For use of ERAS as a standardized program, repeated and expanded research is needed, and a Korean-style ERAS should be prepared by using this approach for various diseases.

창업기업의 지적자본이 혁신성과에 미치는 영향: 고객 참여의 직‧간접효과와 시장변동성의 조절효과를 중심으로

염정원,김경환 대한경영학회 2022 大韓經營學會誌 Vol.35 No.8

The digitalization of all industries and the subsequent start of the 4th Industrial Revolution are having an impact not only on changes within industries but also on the relationship between industries and their customers. As digital communication becomes more advanced, customers knowledge retention increases, greatly resolving the information asymmetry issue. As a result, customer demand rises and customer behavior reflects a pattern of more active participation. Joint innovation activities are being carried out to involve customers in corporate management activities as corporate innovation efforts to cope with rapid business environment changes become more crucial than ever. Academic research on customer participation activities is also being actively conducted. In order for startups experiencing resource limitations to create innovation, it is meaningful for startups’ innovation strategies to examine the relationship between intellectual capital, an internal resource, and the role of customers, an external resource, and the effects of market volatility, an environmental factor. In this study, I researched if customer participation has an impact on the inventive performance of start-ups and a moderating effect of market volatility. I also investigated whether intellectual capital has relevance in developing innovative performance from the perspective of start-ups. In order to analyse the direct and indirect effects of creative performance of customer participation by intellectual capital and type, 307 response data from executives and employees of start-ups during 7 years of establishment were analyzed. As a consequence, it was first established that start-up companies’ intellectual capital is a crucial condition for their success in terms of innovation. Second, intellectual capital as used in all customer engagement activities among start-up companies’ intellectual capital, demonstrating the value of each employee’s personal capital in knowledge-sharing and customer contact. Third, customer participation activities all had a substantial impact on market performance, demonstrating the value that can be created by gathering data that is relevant to the market as a result of customer participation. Fourth, it was discovered that start-up companies’ activities including customer participation in their intellectual capital and innovation performance had a major impact on their performance in the market and their ability to operate effectively. Lastly, it was found that customer participation activities function better and have a bigger impact when the market is more volatile. It is meaningful to suggest the need for customer value and customer utilization strategy as a cooperative innovation partner along with strategic management of intellectual capital to improve innovative performance of start-ups.

중소벤처기업 기술사업화 영향요인 사례연구

염정원,홍세영,김경환 한국기업경영학회 2021 기업경영연구 Vol.28 No.2

글로벌금융위기 이후 세계경제 전반에 걸쳐 나타난 불확실한 경영환경, 새로운 성장패러다임의 등장으로 R&D의 중요성이 커진 가운데, 국내의 연구개발비중도 점차 확대되어 가고 있다. 국내기업 대다수를 차지하는 중소기업의 혁신역량 강화를 위해 정부도 매년 기술사업화 지원규모를 지속적으로 늘려가고 있으나, 상시 내부 자원 부족문제를 겪고 있는 중소기업의 사업화 성공률이 기술개발성공률 대비 저조한 것이 현실이다. 기술사업 화 성과를 창출하기 위한 기업의 영향요인과 유의한 성공요인들을 기존문헌연구를 통해 살펴보고 각 영향요인 별 전략적 대응방안을 대학과 금융회사간의 연계지원한 기업들의 기술사업화 과정에서 확인해보았다. 기술사 업화를 추진하는 과정에서 기업요인, 시장요인, 정책요인간 영향은 각각 분절적으로 기능하기보다는 상호 영향 을 주고 있어 이에 대한 전략적 대응이 주요하다고 볼 수 있다. 본 연구의 시사점은 기업에게 있어서 자체 역량강화와 외부환경에 대한 탐색 뿐만 아니라, 기술사업화 단계별 정부정책을 다양하게 탐색하여 활용하는 것이 내부자원 부족 문제를 해결하면서 효과적으로 기술사업화를 가능하게 한다는 점이다. 아울러 기술사업화 를 지원하는 주체들 간의 협업이 갖는 의미도 크다. 효과적인 상호작용이 이루어지기 위해서는 기업과 시장, 정책의 각 주체들 간의 성과중심적 파트너십 형성이 중요하다. 기술사업화 정책목표를 공유하는 플랫폼에 참여 함으로써 상호의존적인 네트워크를 구성하여 협업의 선순환을 창출한다면, 중복투자의 우려와 창업가들의 탐 색비용을 줄이면서 기술사업화 제고를 도모할 수 있을 것이다. The importance of R&D has increased due to the emergence of a new growth paradigm and the uncertain business environment unfolding throughout the global economy since the global financial crisis, and the proportion of domestic research is also increasing. In order to strengthen the innovation capabilities of SMEs, which account for the majority of domestic companies, the government is also increasing the scale of support for technology commercialization every year. The influencing factors and significant success factors of companies to create technology commercialization outcomes were examined through existing literature studies, and strategic countermeasures for each influencing factor were identified in the process of technology commercialization of companies that supported linkages between universities and financial companies. In the process of promoting technology commercialization, the impacts between corporate, market, and policy factors do not function as a segment, but rather influence each other, so the strategic response to this can be seen as a major factor. The implications of this study are that for entrepreneurs, searching and utilizing government policies in a variety of ways will enable more effective technology commercialization while solving the problem of lack of internal resources. In addition, the significance of collaboration between the main agent supporting technology commercialization is great. In order for effective interaction to get accomplished. it is important to form a performance-oriented partnerships between the main agent of the enterprise, the market, and the policy. If you create a virtuous cycle of cooperation by constituting an interdependent network by participating in a platform that shares the policy goals of technology commercialization, you will be able to promote technology commercialization while reducing the fear of redundant investment and the exploration cost of entrepreneurs.

노인 장루보유자의 영양상태 관련 요인

염정원,신수진 한국노인간호학회 2017 노인간호학회지 Vol.19 No.1

Purpose: The aim of this study was to identify nutritional status of older adults receiving ostomy care and to determine risk factors related to malnutrition Methods: The participants were 122 older adults with an ostomy. Data collection was conducted using a structured questionnaire. Collected data were analyzed using descriptive statistics, x2 test, Fisher's exact test, and logistic regression with the SPSS 22.0 program. Results: Results from Mini Nutritional Assessment showed that for nutritional status, 45.9% of respondents had good nutritional status, 32.0% were at risk for malnutrition and 22.1% were considered malnourished. Factors related to nutritional status were occupation (p=.005), economic status (p<.001), stoma retention period (p=.002), and ostomy care provider (p=.005). The reason for ostomy surgery was the most important determinant of malnutrition (odds ratio 3.27; 95% CI, 1.13~9.49). An additional predictive patient factor was ostomy care provider (OR, 3.07; 95% CI, 1.08~8.76). Conclusion: These results indicate that risk factors related to malnutrition in older patients are the reason for the ostomy surgery and the ostomy care provider. Therefore, in developing nursing education programs for older adults with an ostomy consideration must be given to the characteristics and problems of malnutrition and nutrition interventions.

DNA barcoding of Schisandraceae in Korea

염정원,한상욱,서선원,임채은,오상훈 한국식물분류학회 2016 식물 분류학회지 Vol.46 No.3

The establishment of a DNA barcode database at the regional scale and assessments of the utility of DNA barcodes are crucial for conservation biology and for the sustainable utilization of biological resources. Schisandraceae is a small family consisting of ca. 45 species. It contains many economically important species, such as Schisandra chinensis, which is widely used as a source in tonic beverages and in oriental medicine. In Korea, three species, S. chinensis, S. repanda, and Kadsura japonica, are distributed. We evaluated the level of variation of the DNA sequences of rbcL, matK, and the ITS regions from 13 accessions representing the dis- tributional range of the three species. The three DNA barcode regions were easily amplified and sequenced. The minimum values of the interspecific genetic distances among S. chinensis, S. repanda, and K. japonica either separately or in combination are 4- to 23-fold higher than the maximum value of the intraspecific distance, showing that there is a clear DNA barcoding gap in the regions for Korean Schisandraceae. Phylogenetic anal- yses of the three DNA barcode regions, separately and simultaneously, indicate that all of the DNA barcode regions are useful for identifying a species of Schisandraceae in Korea. The distinctiveness of the three species of Schisandraceae was also supported at the species level when Chinese and Japanese populations were added. The results of this study indicate that three concatenated regions constitute the best option for DNA barcoding in Schisandraceae in Korea.

Rotavirus VP6 유전자의 감자식물체내로의 도입과 형질전환체의 발현분석

염정원,전재흥,정재열,이병찬,강원진,김미선,김철중,정혁,김현순,Youm, Jung-Won,Jeon, Jae-Heung,Jung, Jae-Yeol,Lee, Byoung-Chan,Kang, Won-Jin,Kim, Mi-Sun,Kim, Chul-Joong,Joung, Hyouk,Kim, Hyun-Soon 한국식물생명공학회 2002 식물생명공학회지 Vol.29 No.2

바이러스 설사병의 원인인 VP6유전자를 감자에 형질전환 시키기 위하여 CaMV 35S promoter와 kanamycin 항생제 내성을 갖는 식물발현벡터 pMBP-1에 subcloning하고, 이 재조합 벡터를 A. tumefaciens LBA4404에 도입시킨 후, freeze haw방법을 이용하여 감자에 형질전환 시켰다. 공동배양된 감자의 잎절편은 2,4-D가 2.0 mg/L첨가된 배지에서 2일간 배양 후, 0.01 mg/L NAA, 0.1 mg/L GA$_3$, 2.0 mg/L Zeatin, 100.0 mg/L kanamycin, 500.0 mg/L carbenicillin이 첨가된 선발배지에서 재분화시켰다. 이 때 유도된 신초는 100.0 mg/L의 kanamycin이 포함된 배지에 옮겨준 후, 왕성한 생육을 위해 MS 기본배지에서 다시 배양하였다. 기내배양시 외부유전자의 도입에 의한 외형적인 변화는 찾을 수 없었으며, 형질전환체는 NPT primer를 사용한 PCR방법으로 1차선별 하였다. DIG 표지된 probe를 이용하여 total RNA를 분석한 결과 개체별로 발현양의 차이는 있었으나, 95% 이상의 안정성을 보였고, genomic DNA를 추출해 Southern blot hybridization했을 경우 1~3개의 copy수를 보임으로써 형질전환 식물체에 외래유전자인 VP6유전자가 안정적으로 도입되었음이 확인되었다. A VP6 fragments was subcloned with BamHI in the binary pMBP-1 vector under Califlower Mosaic Virus (CaMV) 355 promoter and neomycin phosphotransferase II (npt II) gene. The recombinant binary vector was mobilized into Agrobacterium-tumefaciens LBA4404 by the freeze-thaw method and potato (Solanum tubensum L. cv Desiree) was transformed by modified leaf-disc cocultivation. Shoots were induced on MS medium with 0.01 mg/L NAA, 0.1 mg/L GA$_3$, 2.0 mg/L Zeatin, 100.0 mg/L kanamycin, 500.0 mg/L carbenicillin. In order to identify the copy number of VP6 into potato plant, total genomic DNA was isolated from transgenic potato and analysed by Southern blotting. Genomic DNA and total mRNA analysis demonstrated the incorporation of the foreign gene into the potato genome, as well as their transcription.

Rotavirus VP6 유전자의 감자식물체내로의 도입과 형질전환체의 발현분석

염정원,강원진,김미선,김현순,김철중,정혁,전재흥,정재열,이병찬 한국식물생명공학회 2002 JOURNAL OF PLANT BIOTECHNOLOGY Vol.29 No.2

유치원 교육실습생에 대한 유아의 생각

염정원,엄정애 이화여자대학교 교육과학연구소 2024 교육과학연구 Vol.55 No.1

본 연구는 유아교육 현장에서 생활하는 유아들이 교육실습생에 대해 어떠한 생각을 가지고 있는지를살펴보고자 하였다. 이를 위해, 2023년 10월 1주에서 4주까지 서울특별시 소재 사계유치원의 만4세, 만5세반 네 학급을 참여관찰하고 해당 학급에 재원 중인 75명의 유아들과 일대일 면담을 실시하였다. 연구결과 첫째, 유아들은 교육실습생을 함께 놀이하는 친구로 인식하며 교육실습생이 학급에서 진행한 대소집단활동 역시 놀이의 일환으로 여기기도 하였다. 둘째, 유아들은 교육실습생이 학급 내에서 일과 전반에 걸쳐 유아들과 담임교사를 도와주는 사람이라고 생각하였다. 셋째, 유아들은 교육실습생이 진짜 선생님인 담임교사에 의해 가르치는 일을 지시받고 이를 일시적으로 수행하기 때문에 진짜 선생님은 아니라고 생각하였다. 넷째, 다수의 유아들은 교육실습생이 비록 잠시 머물렀다 가는 사람이지만 상호 간의 신뢰를 토대로 하여 깊은 정서적인 애착을 느낌으로써 친밀한 타인으로 여겼으며, 일부 유아들은 교육실습생에게 다소 거리감을 느낌으로써 낯선 손님으로 여기기도 하였다. 이상의 결과를 바탕으로 본 연구에서는 유아와 교육실습생이 놀이를 통해 상호작용할 수 있도록 지원하고, 보다 장기적인 관점에서 의미 있는 관계를 맺을 수 있도록 해야 할 필요성을 제시하였다. The purpose of the study was to explore the perspectives of children on kindergarten pre-service teachers. This study was conducted with 75 children, aged 4 and 5 years old who were enrolled in Sagye Kindergarten in Seoul, Korea. The methodology involved one-on-one interviews and participatory observations to capture the children’s thoughts about pre-service teachers. The findings of this study were as follows. First, children thought pre-service teachers as playmates, also considering the group activities organized by pre-service teachers in the classroom as part of play. Second, children perceived pre-service teachers as helpers. They acknowledged that pre-service teachers provided various assistance to them throughout the day, not only to the children but also to the supervising teachers. Third, children recognized that pre-service teachers were not yet full-fledged teachers, because pre-service teachers perform teaching tasks temporarily under the guidance of the supervising teacher. Fourth, many children developed deep emotional attachment and considered pre-service teachers as familiar individuals, highlighting the trust, affection, and intimacy built during these interactions. On the other hand, some children maintained a certain distance from pre-service teachers, perceiving them as somewhat unfamiliar guests. They emphasized the separation of pre-service teachers from the classroom by addressing them differently, and suggested a lack of interaction by mentioning limited experiences of playing together. Based on these findings, the study suggests the importance of supporting interaction between children and pre-service teachers through play and fostering meaningful relationships from a long-term perspective. It also indicates the need for efforts not only from pre-service teachers but also from supervising teachers, educational institutions, and teacher training programs to enhance the quality of these relationships.

항원 생산 기반으로서의 식물 연구

염정원,전재흥,정혁,김현순,Youm, Jung-Won,Jeon, Jae-Heung,Joung, Hyouk,Kim, Hyun-Soon 한국식물생명공학회 2010 식물생명공학회지 Vol.37 No.3

The expression of vaccine antigens in transgenic plants has the potential to provide a convenient, stable, safe approach for oral vaccination alternative to traditional parenteral vaccines. Over the past two decades, many different vaccine antigens expressed via the plant nuclear genome have elicited appropriate immunoglobulin responses and have conferred protection upon oral delivery. Up to date, efforts to produce antigen proteins in plants have focused on potato, tobacco, tomato, banana, and seed (maize, rice, soybean, etc). The choice of promoters affects transgene transcription, resulting in changes not only in concentration, but also in the stage tissue and cell specificity of its expression. Inclusion of mucosal adjuvants during immunization with the vaccine antigen has been an important step towards the success of plant-derived vaccines. In animal and Phase I clinical trials several plant-derived vaccine antigens have been found to be safe and induce sufficiently high immune response. Future areas of research should further characterize the induction of the mucosal immune response and appropriate dosage for delivery system of animal and human vaccines. This article reviews the current status of development in the area of the use of plant for the development of oral vaccines.