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육계(Cortex of Cinnamomum cassia J.Presl) 추출물로부터 부정근 형성 유도물질 분리 및 동정
여주호,백정규,박지셩,김건우 한국자원식물학회 2024 한국자원식물학회지 Vol.37 No.1
적 요본 연구에서 육계 추출물로부터 cinnamyl alcohol을 분리· 정제하였다. 연구 결과, H3 분획100 ㎍/mL에서 부정근 형성율이 286.6%였으며, 표준품 cinnamyl alcohol 1 mM (134.2 ㎍/ mL)의 형성율이 290%로 유사한 결과를 나타내었다. 따라서 육계 methanol 추출물에 함유되어있는 cinnamyl alcohol이 녹두의 부정근 형성을 유도하는 주된 화합물일 것으로 판단된다. 육계에서 동정한 cinnamyl alcohol은 현재 식품첨가제 및 향료로써 사용되고 있으므로 안전성이 이미 보장되어 있으며, 한약재로써 사용된 육계 잔류물을 활용하여 부정근 형성 유도물질을추출한다면비용적인 측면에서 또한 긍정적인 요인으로 작용할것으로 판단된다. 따라서 육계는 유기농 농자재로써 충분히 사용 가능할 뿐만 아니라 신규 천연 식물생장조절제의 개발용 소재로서도 활용 가능할 것으로 사료된다. Abstract - In this study, as a result of exploring the physiological activity of plants useful for agriculture on various plant resources, it was possible to confirm an activity similar to auxin that promotes plant rooting in methanol extract of Cinnamon Bark (cortex of Cinnamomum cassia J.Presl). After separating the active body by applying column chromatography and HPLC to the CHCl3 active fraction obtained by solvent extraction for each polarity from the methanol extract of cinnamon bark, cinnamyl alcohol was identified through GC/MS analysis. By bioassay using cinnamyl alcohol standard and the active fraction separated and purified from the methanol extract of cinnamon bark, the rooting rate of mung bean seedlings of the cinnamyl alcohol standard was 290% compared with the untreated control at 134.2 ㎍/mL concentration, and the adventitious root formation activity similar to the rooting rate (268.6%; 100 ㎍/mL) of the active fraction was shown. In conclusion, it is believed that cinnamyl alcohol contained in methanol extract of Cinnamon Bark is the main compound that induces adventitious root formation in mung bean.
소나무 담쟁이덩굴(Parthenocissus tricuspidata (Siebold & Zucc.) Planch) 줄기 추출물의 항비만 효과
여주호(Joo Ho Yeo),유주형(Yu Ju Hyeong),최민영(Min Yeong Choi),이재원(Jae Won Lee),금나경(Na Gyeong Geum),안미연(Mi-Yun An),정철의(Chuleui Jung),정진부(Jin Boo Jeong) 한국자원식물학회 2022 한국자원식물학회지 Vol.35 No.2
In this study, we investigated in vitro anti-obesity activity of Parthenocissus tricuspidata (Siebold & Zucc.) Planch stem (PTS) in mouse adipocytes 3T3-L1 cells. PTS inhibited lipid accumulation and reduced the expression level of the proteins such as C/EBPα, PPARγ and FABP4 associated with adipogenesis in 3T3-L1 cells. In addition, PTS increased lipolysis-related protein expression such as p-AMPK, ATGL and p-HSL, and reduced perilipin-1 in 3T3-L1 cells. Taken together, PTS may have anti-obesity activity through inhibiting adipogenesis and inducing lipid lipolysis in adipocytes. Based on these results, PTS is expected to be used as a potential functional agent for anti-obesity.
고압수소환경에서 인시츄 SP시험시 수소용 강의 수소취성에 미치는 표면거칠기의 영향 평가
여주호(Ju-Ho Yeo),신형섭(Hyung-Seop Shin),배경오(Kyung-Oh Bae),백운봉(Un-Bong Back) 대한기계학회 2021 대한기계학회 춘추학술대회 Vol.2021 No.11
In order to use steels for hydrogen applications, it is important to secure safety through the evaluation of the hydrogen embrittlement (HE) behaviors. Therefore, it is important to develop a simple test method to examine the HE behaviors under high-pressure hydrogen environment. From this reasons, it is necessary to understand the effect of the surface finish condition of specimens depending on fabrication processes on the HE behaviors. In this study, in addition to austenitic steels, carbon (API-X70 steel) and alloy steels (4Cr-Mo steel) are used for in-situ SP tests in high-pressure environments. The obtained load-displacement curves and absorbed SP energy until gas leakage were obtained. In addition, the relative reduction of thickness (RRT) was evaluated by measuring the thickness of the fractured part of the specimen, and the HE sensitivity depending on the surface roughness was evaluated quantitatively. As a result, it can be found that the effect of the surface roughness of specimens on the HE sensitivity was not clearly observed in both kinds of steels with austenitic and ferritic phases.
섬괴불나무(Lonicera insularis Nakai) 추출물의 면역자극 및 항비만 활성
유주형,여주호,최민영,이재원,금나경,안미연,정진부 한국자원식물학회 2022 한국자원식물학회지 Vol.35 No.4
In this study, we investigated in vitro immuno-stimulatory and anti-obesity activity of fruit (LIF), leaves (LIL) and stems (LIS) from Lonicera insularis Nakai in mouse macrophages RAW264.7 cells and mouse pre-adipocytes 3T3-L1 cells. LIF, LIL and LIS increased the production of immunostimulatory factors such as nitric oxide (NO), inducible nitric oxide synthase (iNOS), interleukin-1β (IL-1β), cyclooxygenase-2 (COX-2), tumor necrosis factor-α (TNF-α) and activated phagocytosis in RAW264.7 cells. Inhibition of toll-like receptor 2/4 (TLR2/4) partly blocked LIF, LIL and LIS mediated production of immunostimulatory factors. In addition, inhibition of mitogen-activated protein kinases (MAPK) signaling attenuated the production of immunostimulatory factors induced by LIF, LIL and LIS. Based on these results of this study, LIF, LIL and LIS is thought to activate macrophages the production of immunostimulatory factors and phagocytosis through toll-like receptor 2/4 (TLR2/4) and MAPKs signaling pathway. In anti-obesity study, LIF reduced the lipid accumulation in 3T3-L1 cells. LIF increased the protein phosphorylation expressions such as AMP-activated protein kinase (AMPK), hormone sensitive lipase (HSL), adipose triglyceride lipase (ATGL) related to the lipolysis of the adipocytes. In addition, LIF increased the expression of proteins involved in energy metabolism and brown adipose tissues differentiation such as peroxisome proliferator-activated receptor gamma coativator 1α (PGC-1α) and PR domain-containing16 (PRDM16). These results suggest that LIF is involved in lipid accumulation inhibition through expressing the proteins such as lipolysis and differentiation of white adipocytes to brown adipocytes. 본 연구에서는 섬괴불나무 열매(LIF), 잎(LIL) 그리고 줄기(LIS) 추출물의 면역증진 활성과 섬괴불나무 열매(LIF) 추출물의 항비만 활성을 평가하였다. 섬괴불나무 열매(LIF), 잎(LIL) 그리고 줄기(LIS) 추출물은 RAW264.7 세포에서 NO, iNOS, COX-2, IL-1β, TNF-α와 같은 면역증진인자의 생성을 증가시켰으며, IL-1β의 발현은 NO생성과 관련된 것으로 보여진다. 면역증진인자은 TLR2/4를 통해 MAPKs중 p38 그리고 JNK를 자극하여 발현이 유도되는 것으로 판단된다. 항비만 실험에서, 섬괴불나무 열매(LIF) 추출물은 AMPK, HSL, ATGL의 발현 증가와 perilipin-1 발현 억제를통해 지질분해를 유도하여 세포 내지질축적을 억제하는 것으로 나타났으며, 갈색지방세포로의분화유도와 에너지 대사에 관여하는 인자인 PRDM16, PGC-1 α의 발현유도를 통해서도 지질축적을 억제하는 것으로 판단된다. 향후 섬괴불나무 추출물은 건강 보조제 및 기능성 식품으로의 활용이 가능할 것으로 판단되지만, 섬괴물나무 추출물의 어떠한 성분이 면역과 항비만 활성에 영향을 미치는지에 대한 성분분석이 필요하다. 또한, 본 연구는 세포를 이용한 실험으로정확한 분석을 위해서는 동물모델을 이용한 섬괴불나무 추출물의 면역증진 및 항비만 활성에 관한 추가적인 연구가 진행되어야 할 것이다