Purification and Characterization of Antibacterial Peptides, Spodopsin Ia and Ib Induced in the Larval Haemolymph of the Common Cutworm, Spodoptera Iitura

최정식,여성문,김웅석,채권석,Choi, Chung-Sik,Yoe, Sung-Moon,Kim, Eung-Seok,Chae, Kwon-Seok,Kim, Hak R. The Korean Society for Integrative Biology 1997 Korean journal of biological sciences Vol.1 No.3

Antibacterial activity was induced in the haemolymph of the common cutworm, Spodoptera litura by the artificial injection of E, coli Ek132. Antibacterial peptides were purified from the immunized haemolymph by heat treatment, ion-exchange chromatography, gel filtration chromatography, and reverse phase FPLC, and their physicochemical characteristics were investigated. These purified antibacterial peptides designated as spodopsin la and Ib were named after Spodoptera litura. Spodopsin la and Ib had the apparent molecular masses of 3, 823 Da and 3, 886 Da, respectively, and about 20% of the sequences had basic amino acids, such as lysine and arginine but no cysteine. Also, spodopsin l was confirmed to be a new member of cecropin family having a similar amino acid sequence to cecropin of lepidopteran insects, such as Bombyx mori and Hyalophora cecropia. The purified spodopsin was active against gram-positive as well as gram-negative bacteria.

별늑대거미(Pardosa astrigera L. Koch) 혈구의 전자현미경적 연구

장병수,여성문,Chang, Byung-Soo,Yoe, Sung-Moon 한국현미경학회 1995 Applied microscopy Vol.25 No.2

The fine structure of the hemocytes in Wolf spider, Pardosa astrigera, is discribed and compared with that of similar cells in other spider species and insects. Five hemocyte types are identified in the hemolymph: prohemocyte, plasmatocyte, granulocyte, spherulocyte and adipohemocyte. Prohemocytes are small with a relatively undifferentiated cytoplasm. The nucleus is comparatively large and has a perinuclear space. Plasmatocytes and granulocytes are pinocytotic function in the hemolymph of the body. The plasmatocytes have some coated pits on the plasma membrane and well developed Golgi complex, The granulocytes appear sequence of events in the formation of coated vesicle from a coated pit on its plasma membrane. Golgi complex become well expressed and give rise to small secretory vesicles which fuse to large bodies. The spherulocytes are larger in cell size than other hemocytes. Their cytoplasm is filled with spherules. The spherules contain the floccurent materials and the helical structured materials, which are 220nm in length and 80nm in width. The adipohemocytes are oval shaped and have a number of lipid droplets.

음용수 불소가 흰쥐태아 법랑모세포의 미세구조에 미치는 영향

임도선,정문진,여성문,Lim, Do-Seon,Jeong, Moon-Jin,Yoe, Sung-Moon 한국현미경학회 1999 Applied microscopy Vol.29 No.2

The response of ameloblast to long term (3 weeks) exposure to fluoride was examined in continuously erupting mandibular incisors of pregnancy rats as compared to control rats receiving a similar diet (Teklad L-356) but no sodium fluoride in there drinking water. Rats were started on water containing 0 ppm, 100 ppm, 200 ppm, and 300 ppm NaF at the beginning of pregnancy. To examine on the ultrastructural changes of the ameloblast, electron microscopy was used. The results indicated that rat incisors expressed two major changes in normal amelogenesis that could be attributed to chronic fluoride treatment. The fluoride produces marked alteration in the fine structure of ameloblast from teeth of young rats, such as large confluent distensions of the endoplasmic reticulum and swelling of isolated mitochondria, in particular on the morphology of the rough-surfaced endoplasmic reticulum. A graded series of alterations to these organelles were produced, and the severity of the changes would seem to be dependent on dose and time. This experimental data suggested that exposure prolonged of animal to high level of fluoride appears to induce morphological changes in the normal appositional growth and initial mineralization of enamel created during amelogenesis. 장기간 투여된 불소가 흰쥐태아의 법랑모세포(ameloblast)에 미치는 영향을 알아보고자 임신한 흰쥐에 100ppm, 200ppm 및 300ppm의 불소가 함유된 음용수를 3주간 투여하였으며, 대조군에는 불소가 함유되지 않은 증류수를 섭취시켰다. 생후 11일이 경과된 어린 흰쥐를 희생하여 하악절치를 발치한 후, 법랑모세포의 미세구조적 변화를 관찰하였다. 법랑모세포의 생활주기인 전분비대, 분비대, 성숙대에서 법랑모세포를 광학 및 투과전자현미경으로 관찰한 결과, 실험군에서는 각 대의 법랑모세포에서 형태 및 구조적인 변형을 나타내었다. 즉, 미토콘드리아 기질 내에서 라멜라 형태의 동심원상의 구조가 평륜됨과 함께 조면소포체가 공포화되고 막성계의 유실로 붕괴가 관찰되었다. 특히 고농도 불소투여군에서는 법랑모세포사이에 세포막이 유실된 세포들이 관찰되었다. 따라서, 임신중인 흰쥐에 투여된 불소는 흰쥐태아의 치아발육과정에서 특히, 법랑질 형성에 관여하는 법랑모세포에 영향을 미쳐 전환주기에 변화를 주었고, 법랑모 세포의 미세구조적 및 형태적 변화를 야기하였다.

고중성지방혈증 및 비만에서 미토콘드리아 DNA D - loop 영역 ( 16289 - 16547 ) 의 다형성

김주성(Ju Sung Kim),여성문(Sung Moon Yoe),오승준(Seung Joon Oh),김성운(Seong Woon Kim),양인명(In Myung Yang),김진우(Jin Woo Kim),김영설(Young Seol Kim),최영길(Young Kil Choi),팽정령(Jeong Ryung Paeng),박혜순(Hae Soon Park) 한국지질동맥경화학회(구 한국지질학회) 2000 韓國脂質學會誌 Vol.10 No.2

N/A N/A

메뚜기 (Euprepocnemis shirakii) 성충의 혈구유형

장병수,한성식,여성문,Chang, Byung-Soo,Han, Sung-Sik,Yoe, Sung-Moon 한국현미경학회 1994 Applied microscopy Vol.24 No.2

Six types of hemocytes were identified in adult grasshopper, Euprepocnemis shirakii Bolivar. The morphological and ultrastructural characteristics of these cells were characterized by phase contrast, scanning and transmission electron microscope. The prohemocytes are small and spherical cells with large nucleus. The plasmatocytes are spindle shaped or polymorphic cells which show numerous cytoplasmic processes on the cell surface and they have lysosomes and vesicles that may be involved in phagocytic function. Especially, multivesicular bodies are observed in the polymorphic cells. The granulocytes I are spherical shaped cells. They are characterized by a number of electron dense granules measuring $0.2-0.3{\mu}m$ in average diameter and marginal band of microtubules which are always in close proximity to the cell membrane. The granulocytes II are oval or spindle-shaped cells. They contain large electron dense granules measuring $0.5-0.8{\mu}m$ in average diameter. Their cytoplasm is filled with numerous granules. The spherulocytes contain large amounts of spherules that most of their cytoplasm. Spherules filled with fine granules or flocculent materials. The oenocytoids are large spherical cells with few cytoplasmic organelles. Their cytoplasm contains peculiar aspect of motochondria and numerous polyrobosome.

메뚜기(Euprepocnemis shirakii Bolivar) 위심세포의 미세구조

余聖文,張炳秀,李成燦,林道善 단국대학교 1993 論文集 Vol.27 No.-

In Euprepocnemis shirakii Bolivar, the pericardial cells are arranged sparsely on each lateral side of the dorsal vessel. The basal lamina of the cell is about 70nm in diameter and functionally adapted for selective filtering of foreign materials from the hemolymph. Cytoplasm of the pericardial cell is structurally divided into two portions. The one is cortex area which consists with labyrinthine channel invaginated into cytoplasm, coated vesicles and α-vacuoles. The other is endoplasm which composed of nucleus, β-vacuoles and γ-vacuoles. The entrance of the labyrinthine canal fromed by invagination of the plasma membrane is junctioned by several membraneous diaphragm folding. So, we guess the membraneous diaphragm be involved in selective absorption of foreign material. Many coated vesicles are formed by pinocytosis of the labyrinthine canal, And these vesicles are then assembled to be α-vacuoles, β-vacuoles, which is present in the outskirts of the endoplasm, have two-folded outer membrane and been stuffed with electron-dense materials. γ-vacuoles consist with one membrane and have mid-electron dense materials in the membrane.

배추흰나비 幼蟲의 味覺感覺器에 관한 走査電子顯微鏡的 硏究

余聖文,張炳秀 단국대학교 1988 論文集 Vol.22 No.-

The gustatory sense organ on the labial and maxillary palps of the fifth instar larval mouthpar of Pieris rapae L. was examined with a scanning electron microscope. The larva has several types of senesory receptor on the distal segments of the labial and maxillary palps. 1. Mandible: None of the sensilla were observed at the mandible. 2. Maixlla: The maxillary palps consist of the base and three segments. Each palp bears a group of about 70 sensilla, indicating a chemoreceptive function, which are located at the apical lobe. The digitiform sensilla, pits, and pegs are scattered around the distal segment of the maxillary palps. 3. Labium: The paraglossae and glossae were not observed at the labium, and labial palps were joined to the prementum. They consist of the base and three segments, and about 60 sensilla are found on the apical lobe of the labial palps. Each gastatory sensillum appears the papilla-like ptojections. Also the digitiform sensilla, pits, and pegs are present at the third segment of the labial palps.

배추흰나비(Pieris rapae L.)의 變態에 따른 여러 器官內 lipase 活性에 관한 硏究

余聖文,朴容一,方仁錫 단국대학교 1990 論文集 Vol.24 No.-

Lipase activities in salivary glands, fat body, and haemolmymph of Pieris rapae L. were measured and compared during metamorphosis, and biochemical properties of lipase in various organs were determined using spectrophotometer UV-240. The temperature and pH optium of lipase in salivary were 30℃ and 8.5 respectively. In fat body and haemolymph pH optima were 8.5 and 8.0 respectively. Lipase activity in salivary glands was maximal in 5th instar larvae declined at Prepupal stage. Lipase activity in fat body was maximal in 5th instar larvae, but drastically reduced at prepupal stage after Which it was increased slowly. Lipase activity in haemolymph was increased continuously from 5th instar larvae to 5day pupae. Metal ions like Hg^++ and Fe^++ acted as an activator, but Ca^++, Mn^++, and Mg^++ did not. Midgut and malpighian tubules did not show any lipase activity while salivary glands, fat, and haemolymph showed lipase activity. Therefore, lipase in Pieris rapae L. is considered to be extra-digestive lipase.

풍화작용에 의한 정상모발의 형태학적 변화

홍완성,장병수,임도선,박상옥,여성문 韓國電子顯微鏡學會 2000 Applied microscopy Vol.30 No.1

성인 남성의 모발에서 정상 모간과 자연상태에서 손상된 모간의 외부형태와 미세구조적 변화를 광학현미경과 주사 및 투과전자현미경으로 관찰하였다. 정상 모간은 모소피층, 모피질, 모수질 등의 3부분으로 구성되어 있었다. 모소피층은 5-7개의 모소피세포들이 중첩되어 모피질과 모수질을 둘러싸고 있고, 길게 신장되어 모피질을 둘러싸고 있는 소피세포의 두께는 약 0.4㎛로 확인되었다. 또한, 이들 세포사이에는 약 25㎛의 세포사이 막복합체가 관찰되었다. 모피질은 잘 발달되어 각질화세포(cornified cell)들로 치밀하게 채워져 있었고, 각질화세포는 직경 약 0.5-0.8㎛인 구형 또는 타원형외 macrofibril로 구성되어 있었으며, 이들은 약 8nm 두께의 가는 microfibri이 동심원상의 규칙적인 형태로 배열되어 있었다. 그리고 macrofibril 사이에는 약 0.5㎛크기의 직경을 가진 구형의 melanin과립들이 고르게 산재되어 관찰되었다. 모수질은 공기가 채워져 있었고, 직경이 약 16㎛의 구형의 형태로 모간의 전체 직경에 1/5 정도를 차지하고 있었다. 풍화에 의해 손상된 모간은 모소피층이 박리되어 있었으며, 모피질세포의 세포질도 손상되어 melanin과립들이 표면에 노출되어 있는 것이 확인되었다. 조직표본상에서 모소피층의 박리과정은 모소피 사이에 공포가 형성되고, 이어서 모소피의 가장자리가 떨어져 나가고, 박리되지 않은 부위는 절단되었다. 모소피가 탈락되어 모피질이 노출된 부위에서는 각질화세포내에 존재하는 macrofibri이 각각 분리되고, 인접원 macrofibril 사이에 공기가 채워지게 되어 오간의 풍화과정은 더욱 촉진되었다. 그리고 노출된 macrofibril 사이에서 탈락 직전의 melanin 과립들이 산재되어 관찰되었다. The morphological changes in normal and weathering hair shafts of the human scalp were investigated by using the transmission and scanning electron microscopes. The hair shaft composed of cuticular layer, cortex and medulla. The surface of normal hairs are smooth and covered by imbricated cuticular scales. The cuticular layer consists of five to seven cuticle cells. These cells, which are flat and thin, measuring about 100 ㎛ long and 0.4 ㎛ thick, appears intercellular membrane complex in diameter 25 nm. The cortex composed of melanin granules and cornified cells, which multicomponent concentric microfibrils in diameter about 8 nm give rise to macrofibrils in diameter 0.5 ㎛ to 0.8 ㎛ encased in limiting membrane. The melanin granules are spherical shaped about 0.5 ㎛ in size and scattered between macrofibrils. The medulla in the normal hairs are 16 ㎛ in diameter centrally region of cortex. Normal hair shafts undergo progressive degenerative changes due to a variety of environmental insults. In the initial weathering process of hair, the cuticular scales became irregularly raised and broken, and then cuticle cells formed cytoplasmic vacuolation, following dissociated intercellular membrane complex, ultimately entirely lost and nuded cortex. Occasionally, transverse fissures were seen at hair shafts indicating that the hairs were deteriorated Complete removal of the cuticular layer in the heavily damaged cortex portions appeared splitting of the cortical cell into its macrofibrils and scattering of melanin granules.

고중성지방혈증 및 비만에서 미토콘드리아 DNA D-loop 영역(16289-16547)의 다형성

김주성,여성문,오승준,김성운,양인명,김진우,김영설,최영길,팽정령,박혜순 한국지질학회 2000 韓國脂質學會誌 Vol.10 No.2