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腦脊髓液内의 Norepinephrine,Epinephrine 및 Dopamine 의 定量法
孫昌福,高炳鶴,白汝基 대한신경정신의학회 1982 신경정신의학 Vol.21 No.3
A direct rapid radioenzymatic assay for measuring simultaneously picogram quantities (2—5 pg) of norepinephrine, epinephrine and dopamine by a high pressure liquid chromatography has been developed. Fifty micrcliters of CSF was incubated with catechol-O-methyltransferase and S-adencsyl-L- 〔methyl-sH〕methionine to convert the three catecholamines to their O-methylated analogues. These derivatives were purified by the solvent extraction and isolated by high pressure liquid chromatography using ᄍ-Bondapak-Cis column and 0.17 M acetic acid-5% methanol—0.02 M Na2 SO4 as eluant. When the integration mark was automatically placed on the chart of data module, manual collection was started two seconds later, and each peak was collected. The norepinephrine and epinephrine derivatives fractions were oxidized to vanillin, and then the solvent extraction of the vanillin and the dopamine derivative fractions were performed and counted by liquid scintillation. A rapid high pressure liquid chromatographic separation of the three catecholamine derivatives, together with the above oxidation and the solvent extraction contributes to high sensitivity and specificity of the method and makes us to complete the differential analysis of 10 CSF samples (20 tubes) within one day. In case of norepinephrine assay alone, more than 30 samples can be completed in a day.
백승필,민기하,장의경,여기백,기미란 한국공업화학회 2014 한국공업화학회 연구논문 초록집 Vol.2014 No.1
Carbonic anhydras (CA) is a potential biocatalyst for CO<sub>2</sub> sequestration because of its ability to catalyze the conversion of CO<sub>2</sub> to bicarbonate. High production and immobilization of functional CA are required for practical application of enzymatic CO<sub>2</sub> capture system. In previous report, α type-CA (HC-aCA) of Hahella chejuensis KCTC was mostly produced as insoluble form in E. coli expression system. Here, by removal of the signal peptide (sp), we successfully set up soluble expression systems. Under the optimized conditions, 30 mg/L of purified HC-aCA (sp-) was obtained. HC-aCASP(-) displayed high pH stability in alkali condition with maximal activity at pH 10. In addition, we chemically immobilized the HC-aCA(sp-) on silica particles. HC-aCASP(-) immobilized silica particle activity retained above 90% even after 10 times reuse.
Immobilization and characterization of carbonic anhydrase with monosilicate and spermine
백승필,손려강,기미란,여기백,민기하 한국공업화학회 2014 한국공업화학회 연구논문 초록집 Vol.2014 No.1
A chemical absorption technology using amine aqueous solution to capture CO<sub>2</sub> causes by-product due to harsh condition process. It is an effective eco-friendly way to replace the amine solution with biocatalyst. But the CO<sub>2</sub> sequestration process is worked at harsh condition We found Hahella chejuensis carbonic anhydrase (HCCA) derived from domestic marine bacterium having high pH stability. To adjust CO<sub>2</sub> sequestration system, long time thermal stability and recyclable are necessary. We performed HCCA immobilization system based on silicification of a monosilicate, tetramethyl orthosilicate (TMOS), and spermine derived from organic compound having 4 amine group. Free HCCA lost their activity in 1 hour at 60 degree, in contrast, immobilzed HCCA by silica particle endured over 2hour with 80% activity. In 13 times recycling test, immobilized HCCA activity was maintained 80%. Thus monosilicate immobilization of HCCA is ready to adjust CO<sub>2</sub> sequestration system.
김성호(Sung Ho Kim),여기백(Ki Baek Yeo),박민규(Min Kyu Park),박종순(Joung Soon Park),기미란(Mi Ran Ki),백승필(Seung Pil Pack) 한국생물공학회 2015 KSBB Journal Vol.30 No.6
3D printing technology has been used in various fields such as materials science, manufacturing, education, and medical field. A number of research are underway to improve the 3D printing technology. Recently, the use of 3D printing technology for fabricating an artificial tissue, organ and bone through the laminating of cell and biocompatible material has been introduced and this could make the conformity with the desired shape or pattern for producing human entire organs for transplantation. This special printing technique is known as “3D Bio-Printing”, which has potential in biomedical application including patient-customized organ out-put. In this paper, we describe the current 3D bio-printing technology, and bio-materials used in it and present it"s practical applications.