http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
생물 유기분자 유도 실리카 합성실험을 통한 생체내 유무기 복합체 형성이해
기미란,민기하,백승필 한국생물공학회 2019 KSBB Journal Vol.34 No.4
The discovery of the principle molecules involved in biosilicification both in diatoms and sponges brings out a new paradigm for silica synthesis at environmentally benign conditions. Here we introduce the biosynthetic method of silica using organic molecules for the undergraduate students. First, the biosilica synthesized was assessed through both a colorimetric method for quantification of silica and structural analysis by SEM image observation. Next, facile immobilization of an enzyme was demonstrated. Specifically, bovine carbonic anhydrase II (bCAII) was efficiently immobilized within silica matrix by simply adding itself to the reaction mixture containing polyamines and tetramethyl orthosilicate in phosphate buffer, obtaining 97% immobilization to initial amount of bCAII. The immobilized bCAII retained 40% of initial activity at 90℃ whereas free form did less than 10% of initial activity at 70℃ and the total yield from the 5 times reuses of immobilized bCAII was twice that of free bCAII. This experimental content that is hard to find in general biology or biochemistry lab could contribute to students' interest in knowledge expansion and application of new materials.
백승필,민기하,장의경,여기백,기미란 한국공업화학회 2014 한국공업화학회 연구논문 초록집 Vol.2014 No.1
Carbonic anhydras (CA) is a potential biocatalyst for CO<sub>2</sub> sequestration because of its ability to catalyze the conversion of CO<sub>2</sub> to bicarbonate. High production and immobilization of functional CA are required for practical application of enzymatic CO<sub>2</sub> capture system. In previous report, α type-CA (HC-aCA) of Hahella chejuensis KCTC was mostly produced as insoluble form in E. coli expression system. Here, by removal of the signal peptide (sp), we successfully set up soluble expression systems. Under the optimized conditions, 30 mg/L of purified HC-aCA (sp-) was obtained. HC-aCASP(-) displayed high pH stability in alkali condition with maximal activity at pH 10. In addition, we chemically immobilized the HC-aCA(sp-) on silica particles. HC-aCASP(-) immobilized silica particle activity retained above 90% even after 10 times reuse.
Shipra Kumari,민기하,KANTH BASHISTHA KUMAR,장의경,백승필 한국생물공학회 2020 Biotechnology and Bioprocess Engineering Vol.25 No.5
Biogenic TiO2 diatoms can be prepared by metabolic incorporation of TiO2 on the frustule surface of the diatoms. To utilize the photo-catalytic activity of biogenic TiO2 for practical applications, high production of TiO2- deposited diatoms is required. Here, we evaluated the growth rate and biogenic TiO2 production of several domestic diatom species. Among the tested diatoms, Caloneis schroederi (CS) showed high biomass production (4.01 g/L) in a twostage cultivation system and proper exoskeletal rigidity for efficient production of biogenic TiO2. The dry weight of the TiO2-deposited CS frustules (biogenic CS-TiO2) was 4.52 g/L. Biogenic CS-TiO2 showed significant photocatalytic degradation of Congo Red in water with an efficiency of 27.8%, which is higher than that of standard TiO2 (17.4%). In addition, chloroform could be removed from water through the photo-catalytic activity of biogenic CS-TiO2 (91%). Therefore, the biogenic CS-TiO2 developed in this study can be employed for the treatment of pollutantcontaminated wastewater.