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安武植 慶北大學校 醫科大學 1980 慶北醫大誌 Vol.21 No.2
尿路系의 長期入院患者 49名에서 57例의 尿를 採取하여 菌培養을 하였던바 46例(81%)에서 10^5/㎖ 以上의 菌이 檢出되었다. 이들 患者는 膀胱의 破裂, 損傷 其他 尿路疾患이 36例였고 13例는 他疾患에 의한 排尿障碍가 있어 導尿하고 있는 患者였다. 尿中 菌陰性例는 發病後 1∼3個月以內의 患者이며 導尿를 하지 안한 患者에서는 8∼11個月이 되어도 菌陰性이었다. 同一尿中에서 1∼3種의 菌이 檢出되었다. 分離菌 76洙中 Pseudomonas 屬이 24株로 가장 많았고 Klebsiella 및 Serratia, Acinetobacter, Enterobacter, Citrobacter, E. coli, Proteus의 順으로 적어지며 Gram 陽性菌으로는 Staphylococcus만이 6株였다. 混合感染에 關與하는 菌 亦是 Pseudomonas가 가장 많았고 Klebsiella, Serratia 및 Acinetobacter도 比較的 많았다. 分離菌의 51%가 amikacin에 耐性이었고 trimethoprim, nalidixic acid, refampin의 順으로 耐性菌이 많아지나 70% 以下였고 其他抗菌劑에는 77∼91%의 菌이 耐性이었다. Staphylococcus는 cloxacillin, cephaloridine 및 minocycline에는 全部가 感受性이며 trimethoprim에는 1株만이 耐性이었으나 其他抗菌劑에는 大部分이 耐性이었다. 同一患者에서 反復하여 檢尿한 例에서의 菌種의 變動을 보면 Pseudomonas와 Klebsiella은 계속해서 分離되나 其他菌種은 交替되는 傾向이 있었다. Fifty-seven urines collected from 49 long-term inpatients suffering from diseases related to urology clinics were cultured bacteriologically, and 46 (81%) urines were found to be infected with more than 10^5 microorganisms per ㎖. These patients consisted of 36 cases suffering from rupture or damage in bladder, and other urologic diseases, and 13 cases suffered voiding difficulty due to diseases unrelated to urologic diseases. Catheterized patients were culture negative during the period up to one to three months after the onset of diseases, and non-catheterized ones were negative even after 8 to 11 months of diseases. Mixed infections with two to three species of microorganisms were found in about a half of contaminated urines. Among 76 strains of microorganisms isolated, Pseudomonas species were most frequently identified with 24 strains, and the number decreased in the following order: Klebsiella and Serratia, Acientobacter, Enterobacter, Citrobacter, E. coli, and Proteus. Only six strains of Gram positive bacteria (Staphylococcus) were isolated. Approximately a half of isolated organisms were resistant to amikacin, and the number of strains resistant to drugs decreased in the following order of trimethoprim, nalidixic acid, and rifampin, but they were not more than 70%. Approximately 77 to 91% of organisms were resistant to the other drugs tested. All Staphylococcus were susceptible to cloxacillin, cephaloridine, and minocycline, and only one was resistant to trimethoprim. Almost all of them were resistant to the other drugs including methicllin. Repeated cultures of urines from same patients showed a tendency of changing patterns in bacterial species, but Pseudomonas and Klebsiella persisted throughout the study in almost all cases.
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安武植,鄭在奎,全燾基 慶北大學校 醫科大學 1979 慶北醫大誌 Vol.20 No.2
The activity of various antifungal agents to standard and isolates of Candida sp. were studied. Minimum inhibitory cocentrations (MICs) of gentian violet ranged from 2.5 to 5㎍/ml, and those of nystatin were from 1.6 to 3.3㎍/ml to standard strains, but commercially available topical agents, such as siccarin, canesten and miconazole, were inactive with MICs of 64 to 128㎍/ml. MICs of gentian violet and nystatin to our isolates were almost the same as those to standard strains, but 8 susceptible strains to canesten and five susceptible strains to miconazole were found among 40 strains. Some our isolates of C. guillermondii were also susceptible to the topical antifungal agents tested. Nystatin revealed strong fungicidal action to most Candida sp. but not to C. krusei, whereas miconazole nitrate showed weak fungicidal action to Candida sp.
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鄭在奎,安武植 慶北大學校 醫科大學 1979 慶北醫大誌 Vol.20 No.1
試驗管內 difolafan의 抗菌作用을 Candida, Cryptococcus, Sporotrichum, Trichophyton 等 諸菌腫에 對하여 檢査하여 他種 藥劑와 比較하였다. 이 物質은 Candida腫 特히 Trichphyton mentagrophytes 및 Trichophyton rubrum에 對해서 强한 發育抑制作用을 나타내었으며 現用의 治療劑보다 월등 强力하였으며, Cryptococcus 및 Sporotrichum腫에 對해서도 抗菌作用을 나타내었다. 殺菌作用에 있어서도 이들 菌腫에 對해서 phenylmercuric acetate 보다 多少 弱하나 miconalze 보다는 顯著히 强한 殺菌力을 發揮하였다. (本 硏究의 一部는 1978年度 文敎部 學術硏究 造成費에 依하였음). The antifungal effects of difolatan (N-(tetrachlorethyl thio)-4-cyclohexene-1,2-dicarboximide), an agricultural fungicidal compound, were evaluated in vitro on the common pathogenic fungi found in Korea such species as Candida, Cryptococcus, Sporotrichum and Trichophyton, and these actions were compared to the currently available therapeutic durgs. This compound exhibited a stronger funglistatic effects against Candida species and especially against both species of Trichophyton mentagrophytes and Trichophyton rubrum than any kind of therapeutic agents, also showed a moderate susceptibility to some strains of Cryptococcus neoformans and Sporotrichum schoenckii. Although the fungicidal action of difolatan was somewhat less strong than phenyl-mercuric acetate, still for stronger than miconazple nitrate against the strains tested.
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