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붉나무 (Rhus javanica) 가지 추출물의 항노화 및 항염증 효과
안대성 ( Dae Sung An ),서수정 ( Su Jeong Seo ),김남우 ( Nam Woo Kim ),이양숙 ( Yang Suk Lee ) 대한미용학회(구 대한미용과학회) 2017 대한미용학회지 Vol.13 No.2
Anti-aging and anti-inflammatory activities of water extract (WE) and ethanol extract (EE) of Rhus javanica branches were investigated for application as cosmetics and beauty industrial materials. The collagenase inhibition rate for anti-wrinkle effects was 44.02% (WE) and 59.95% (EE) at a concentration of 1.0 mg/mL. The elastase inhibition activities of WE and EE were 69.29% and 80.66%, respectively. Measurements of the tyrosinase inhibition rate revealed 22.30% and 23.30% in WE and EE, respectively. In the cytotoxicity test of the extracts on RAW264.7 macrophages and HaCaT keratinocytes using the MTT assay, RAW264.7 macrophages viability decrease was 17.65% in the WE at 10 μg/mL, EE did not show cytotoxicity on RAW264.7 cells at the test concentration. HaCaT cell viability decreased in WE (4.33%) and EE (11.55%) at 10 μg/mL, but no cytotoxicity was observed over 20 μg/mL concentration. For xanthine oxidase inhibition, WE showed the highest value 50.42% at the concentration of 1.0 mg/mL, while the activity from EE was 47.96%. The inhibitory effect on NO production in LPS-stimulated RAW264.7 cells decreased with increasing concentration. The production of TNF-α and IL-6 were suppressed, and the production of IL-6 was inhibited over 60% after treatment with 100 μg/mL EE. These results suggested that R. javanica branches has anti-aging and anti-inflammatory properties and can be used as a natural source for the development of functional cosmetically agents and beauty industry materials.
우산나물(Syneilesis palmata) 추출물의 항산화 활성
이양숙(Yang-Suk Lee),안대성(Dae-Sung Ahn),주은영(Eun-Young Joo),김남우(Nam-Woo Kim) 한국식품영양과학회 2009 한국식품영양과학회지 Vol.38 No.11
기능성식품 소재를 개발하기 위한 연구의 일환으로 야생산채류인 우산나물의 지상부와 뿌리 추출물에 대한 플라보노이드와 폴리페놀 화합물 함량 그리고 생리활성 효과를 측정하였다. 플라보노이드는 지상부 에탄올 추출물이 31.72 ㎎/g으로 가장 많았으며, 폴리페놀은 뿌리의 에탄올 추출물에서 68.11 ㎎/g으로 가장 높았다. Xanthine oxidase에 대한 저해 효과를 측정한 결과 1.0 ㎎/mL의 농도에서 지상부 물 추출물과 뿌리의 에탄올 추출물 모두 98% 이상의 저해율을 보였으며, tyrosinase 저해는 뿌리 에탄올 추출물에서만 9.33%의 저해효과를 나타내었다. 우산나물 뿌리의 에탄올 추출물은 pH 1.2의 조건에서 70.89%의 아질산염 소거능을 보였으며, SOD 유사활성능도 13.06%의 활성을 나타내었다. 또한 전자공여능에서도 뿌리 에탄올 추출물이 98.58%로 천연항산화제인 ascorbic acid보다 높은 활성을 보였다. 이상의 결과 우산나물 뿌리의 에탄올 추출물의 폴리페놀 함량이 가장 높았고, tyrosinase 저해, 아질산염 소거, SOD 유사활성 및 전자공여능이 가장 우수하였다. 우산나물 뿌리의 물 추출물은 에탄올 추출물보다는 활성이 낮았으나 우산나물 지상부 추출물보다 높게 분석되었으며, xanthine oxidase 저해율이 가장 높았다. 본 실험결과 우산나물은 우수한 항산화 활성을 지닌 기능성식품 소재임을 확인할 수 있었으며, 식용으로 사용하고 있는 어린순 이외에 뿌리에도 다량의 폴리페놀을 함유하며, 우수한 생리활성 효과를 나타내므로 이를 항산화 활성을 지닌 기능성 건강식품 등의 재료로 활용할 수 있을 것으로 생각된다. This study was conducted to examine the antioxidant activities of the extracts from aerial parts and roots of Syneilesis palmata. The ethanol extract of aerial parts showed the highest content of flavonoid compounds (31.72 ㎎/g), and the ethanol extract of roots has the highest content of total polyphenol compounds (68.11 ㎎/g). The water extract of S. palmata roots showed the highest xanthine oxidase inhibition of 99.29% and the ethanol extract of aerial parts showed 98.48% at 1.0 ㎎/mL. The ethanol extract of roots showed the highest value of nitrite scavenging ability of 70.89% at pH 1.2, SOD-like activity of 13.06% and electron donating ability of 98.58% at 1.0 ㎎/mL concentration. The effect of tyrosinase inhibition was found at only the ethanol extracts of roots (9.33%). We found that root extracts contain abundance polyphenol compounds and their antioxidant activities were greater than those of the extracts of aerial part.
한국 동해안 독도의 해조상 및 대황(Eisenia bicyclis) 부착기의 생물상1a
박서경 ( Seo Kyoung Park ),이정록 ( Jung Rok Lee ),허진석 ( Jin Suk Heo ),안대성 ( Dae Sung An ),이행필 ( Aeng Pil Lee ),최한길 ( Han Gil Choi ) 한국환경생태학회 2014 한국환경생태학회지 Vol.28 No.6
독도 해역의 해조류는 2013년 5월과 7월에 14개 정점에서 정성채집 하였으며, 대황의 생태학적 역할을 확인하기 위하여 7월과 11월에 23개체를 채집하여 부착기에 서식하는 해조류와 동물을 동정하였다. 대황의 줄기길이와 무게의 회귀식으로 생물량을 측정하였다. 해조류는 녹조류 18종, 갈조류 35종과 홍조류 75종을 포함하여 총 128종이 관찰되었으며, 성긴분기형이 47.66%로 가장 우점하였고 안정된 환경에 서식하는 ESG I그룹에 속하는 종이 91종(71.09%)이었다. 또한, R/P, C/P, (R+C)/P는 2.14, 0.51과 2.66으로 온대성과 혼합성 해조상을 보였다. 대황의 생물량은 23.74kgm-2이고 밀도는 64 fronds m-2로 확인되었다. 한편, 대황의 부착기에서 해조류 12종과 동물 83종(환형 15종, 연체25종, 절지 34종, 극피 3종, 기타 6종)의 1,248개체가 동정되어 생태학적으로 매우 중요한 핵심종으로 확인되었으며, 넓은마디육질꼬리옆새우붙이가 538개체(43.11%)로 가장 우점하였다. 본 연구 결과 독도의 해조류 출현종수는 이전연구에 비해 증가하였고 성긴분기형과 ESG I 해조류가 우점하는 것으로 나타나 현재까지 독도 연안의 환경은 매우 안정된 상태라고 판단된다. Seaweeds were identified after qualitative sampling at 14 stations of Dokdo coasts from May to July 2013 and seaweeds and animals inhabiting 23 Eisenia bicyclis plants were examined to evaluate its ecological role. Biomass was calculated by using regression between stipe length and weight of E. bicyclis. A total of 128 species were identified, including 18 green, 35 brown, and 75 red algae. Coarsely branched form was dominant functional group occupying 47.66% and 91 species (71.09%) were in ESG I group, growing in stable environmental coast. Also, R/P, C/P, and (R+C)/P were 2.14, 0.51, and 2.66, respectively showing temperate and mixed flora. Biomass and density of E.bicyclis were 23.74kg m-2 and 64 fronds m-2. Twelve seaweeds and 83 animal species (15 Annelida, 25 Mollusca, 34 Arthropoda, 3 Echinodermata, and 6 others) were observed from 23 holdfasts and Ericthonius pugnax was dominant taxon having 538 (43.11%) of 1,248 animal individuals. These results indicate that E.bicyclis is a keystone species showing very important ecological role. In conclusion, the number of seaweeds increased because of intensive research and dominance of coarsely branched form and ESG I group seaweeds, representing that environmental condition of Dokdo is still intact.
의사위성을 이용한 광역보정정보 전송시스템에 대한 실시험
권금철(Keum-Cheol Kwon),양철관(Cheol-Kwan Yang),윤기영(Ki-Young Yun),이정훈(Jung-Hoon Lee),안대성(Dae-Sung An),김강토(Kang-To Kim),심덕선(Duk-Sun Shim) 대한전기학회 2014 정보 및 제어 심포지엄 논문집 Vol.2014 No.10
In this paper, results of real-time experiments of WA-DGNSS transmission system using pseudolite are presented. Correction data and integrity information is transmitted to users by network communication between master station and pseudolite. And performance of WA-DGNSS transmission system is tested by software and hardware GPS receiver. By results of the experiments, we show that correction data is well transmitted to GPS receiver without errors and the navigation error is decreased.
쇠미역(Costaria costata)과 미역(Undaria pinnatifida) 배우체의 생장과 성숙에 광과 온도가 미치는 영향
나연주 ( Yeon Ju Na ),전다빈 ( Da Vine Jeon ),한수진 ( Su Jin Han ),( Cyr Abel Ogandaga Maranguy ),안대성 ( Dae Sung An ),차형기 ( Hyung Kee Cha ),이재봉 ( Jae Bong Lee ),양재형 ( Jae Hyeong Yang ),이해원 ( Hae Won Lee ),최한길 한국수산과학회(구 한국수산학회) 2016 한국수산과학회지 Vol.49 No.2
We examined for differences in the growth and fertility of microscopic stages between Costaria costata and Undaria pinnatifida in crossed temperature×irradiance (12, 17, 22℃×20, 60 μmol photons m-2s-1) and daylength×irradiance (8, 12, 16, 24 h×20, 60 μmol photons m-2s-1) experimental designs. After 13 days in culture, maximum gametophyte growths of the two species were obtained under the following combinations of factors: 17℃×60 μmol photons m-2s-1 and 24 h daylength×60 μmol photons m-2s-1. C. costata gametophytes produced sporophytes about 6 days earlier than those of U. pinnatifida. Sporophyte densities were highest in the 12 h daylength×60 μmol photons m-2s-1 combina-tion after 20 days in C. costata (9.7 plants mm-2) and 26 days in U. pinnatifida (9.1 plants mm-2). However, optimal growth conditions for the microscopic sporophytes differed between species: 17℃×60 μmol photons m-2 s-1 for C. costata, 12℃×60 μmol photons m-2 s-1 for U. pinnatifida. Thus, C. costata gametophytes grew faster and produced sporophytes earlier than those of U. pinnatifida, even though the haploid gametophytes of the two species responded similarly to environmental conditions. Optimal growth temperatures for the microscopic sporophytes also differed between species.