구강편평세포암에서 telomerase 활성도의 임상적 연관성에 관한 연구

심유진,김명진,남동석,이종호,Shim, Yu-Jin,Kim, Myung-Jin,Nahm, Dong-Seok,Lee, Jong-Ho 대한구강악안면외과학회 2001 대한구강악안면외과학회지 Vol.27 No.4

Telomerase is a ribonucleoprotein that synthesizes telomere repeats. It has been reported that activation of telomerase was associtated with immortalization, proliferative activity and carcinogenesis. Recently, telomerase activity has been extensively studied in many kinds of malignant tumors for clinical diagnostic and/or prognostic utilities. In neuroblastoma, breast carcinoma, gastric carcinoma, non-small cell lung carcinoma, close relationship has been reported between high telomerase activity and lymph node metastasis, tumor aggressiveness and poor prognosis. The purpose of this study is to investigate the clinical implication of telomerase activity assay as an adjunctive factor in decision-making on neck node management, speedy pre-operative judging on histologic malignancy grading. Thus we performed semi-quantitative assay of telomerase activity using Telomerase PCR ELISA $kit^{(R)}$(Boeringer Manheim, Germany) and evaluated correlation between telomerase activity and tumor size, neck node metastasis, Anneroth malignancy score and influence of pre-operative chemotherapy on its activity in 27 cases of oral squamous cell carcinomas and 18 cases of normal oral epithelium. Also, correlation between telomerase activities and PCNA indices was evaluated. The results were obtained as follows: 1. The telomerase activities were detected in 24 specimens out of 27 oral squamous cell carcinoma specimens (88.9%) and in 5 specimens out of 18 normal oral epithelium specimens (27.8%). The mean value of telomerase activities was $0.9793{\pm}0.3428$ in 24 oral squamous cell carcinoma specimens and $0.4855{\pm}0.1117$ in 5 normal oral epithelium specimens. The positivity rate and mean value of telomerase activities in oral squamous cell carcinoma specimens were significantly higher than those of normal oral epithelium specimens (p<0.05). 2. There was no significant correlation between total Anneroth malignancy score and telomerase activity (p>0.05), but points of mitosis index and depth of invasion were significantly correlated with telomerase activities (p<0.05). 3. The positive immunohistochemical staining for PCNA(proliferating cell nuclear antigen) was observed in 26 specimens out of 27 oral squamous cell carcinoma specimens and mean value of PCNA indices of 26 specimens was $53.67{\pm}26.46$. PCNA indices were significantly correlated with telomerase activities (p<0.05). 4. The mean value of telomerase activities was significantly higher in pathologic T3/T4 group than in T1/T2 group (p<0.01). There was no significant difference of mean value of telomerase activities between pathologic neck node positive group and negative group (p> 0.05). Pre-operative chemotherapy significantly lowered the telomerase activities (p<0.05). The above results suggested telomerase activity could be used as diagnostic marker and adjunctive parameter for judging on histologic malignancy in oral squamous cell carcinoma.

음성인식을 이용한 자동 호 분류 철도 예약 시스템

심유진,김재인,구명완,Shim Yu-Jin,Kim Jae-In,Koo Myung-Wan 대한음성학회 2004 말소리 Vol.52 No.-

This paper describes the automated call routing for train ticket reservation aid system based on speech recognition. We focus on the task of automatically routing telephone calls based on user's fluently spoken response instead of touch tone menus in an interactive voice response system. Vector-based call routing algorithm is investigated and mapping table for key term is suggested. Korail database collected by KT is used for call routing experiment. We evaluate call-classification experiments for transcribed text from Korail database. In case of small training data, an average call routing error reduction rate of 14% is observed when mapping table is used.

음성인식을 이용한 고객센터 자동 호 분류 시스템

심유진,김재인,구명완,Shim, Yu-Jin,Kim, Jae-In,Koo, Myung-Wan 한국음성학회 2005 음성과학 Vol.12 No.2

This paper describes the automated call routing for call center system based on speech recognition. We focus on the task of automatically routing telephone calls based on a users fluently spoken response instead of touch tone menus in an interactive voice response system. Vector based call routing algorithm is investigated and normalization method suggested. Call center database which was collected by KT is used for call routing experiment. Experimental results evaluating call-classification from transcribed speech are reported for that database. In case of small training data, an average call routing error reduction rate of 9% is observed when normalization method is used.

ROCK 억제제를 통한 사람 치유두 조직 유래 단일 사람 유도만능줄기세포의 생존성 향상

심유진(Yoo-Jin Shim),강영훈(Young-Hoon Kang),김현지(Hyeon-Ji Kim),김미정(Mi-Jeong Kim),이현정(Hyeon-Jeong Lee),손영범(Young-Bum Son),이성호(Sung-Ho Lee),전병균(Byeong-Gyun Jeon) 한국생명과학회 2019 생명과학회지 Vol.29 No.8

이 연구는 단일 세포로 분리된 유도만능줄기세포(induced pluripotent stem cells, iPSCs)에 anoikis 세포사멸을 억제할 수 있는 Rho-associated protein kinase (ROCK)의 억제제를 처리하여 iPSCs의 세포 생존성을 향상하고자 하였다. Episomal plasmid 방법으로 확립된 iPSCs를 단일세포로 분리한 후, ROCK 억제제 Y-27632 dihydrochloride (Y-27632)를 0 uM, 0.5 uM, 1 uM, 2.5 uM, 5 uM, 7.5 uM 및 10 uM 농도별로 5주일 동안 각각 처리하였을 때, 5 uM 이상의 농도에서 세포의 생존율이 유의적으로 향상되었고, 10 uM의 Y-27632을 0일, 1일, 2일, 3일, 4일 및 5일 동안 처리하였을 때, Y-27632의 노출 기간이 길어질수록 세포의 생존율이 유의적으로 향상되는 것을 관찰하였다. 그러나, Y-27632의 노출 후, iPSCs의 형태학적 분화가 관찰되어 10 uM의 Y-27632에서 5일 동안 iPSCs 에 처리 한 후, 줄기세포학적인 특성을 비교 조사하였다. 우선, octamer-binding transcription factor 4 (OCT-4), homeobox protein NANOG (NONOG) 및 SRY-box 2 (SOX-2) 줄기세포 특이 유전자의 발현은 Y-27632를 처리한 실험군은 Y-27632를 처리하지 않은 대조군에서 서로 유의적인 차이를 나타내지 않았다. 또한, Y-27632를 처리한 실험군은 Y-27632를 처리하지 않은 대조군과 비교하여 telomerase 활성과 이것의 활성과 관련된 telomerase reverse transcriptase (TERT) 및 telomerase RNA component (TERC)의 유전자 발현에는 유의적인 차이가 없었다. 이상의 결과로 보아, iPSCs에 Y-27632를 처리하였을 때, iPSCs의 줄기세포의 특정을 유지하면서 anoikis에 의한 세포사멸을 감소시켜 세포 생존율이 증가한다는 것을 알 수 있었다. The aim of the present study was to improve the cell viability of human dental papilla derived single-induced pluripotent stem cells (iPSCs) using a Rho-associated protein kinase (ROCK) inhibitor, Y-27632. The iPSCs were produced using an episomal plasmid-based reprogramming method. After cell separation using trypsin, the iPSCs were treated with 0, 0.5, 1, 2.5, 5, 7.5, or 10 μM Y-27632 for 5 d. Cell viability increased significantly following the 5 μM Y-27632 treatment (p<0.05). When the iPSCs were exposed to medium containing 10 μM Y-27632 for 0, 1, 2, 3, 4, and 5 d, the cell viability rate increased significantly in accordance with the cell viability rate (p<0.05). To evaluate the effect of the Y-27632 treatment on stemness characteristics, the expression of stem cell-specific transcripts and telomerase activity were investigated in the iPSCs treated with 10 μM Y-27632 for 5 d. The expression levels of stem cell-specific transcripts, such as OCT-4, NONOG, and SOX-2, and telomerase activity were not significantly different in the iPSCs treated with 10 μM Y-27632 as compared with those of untreated control iPSCs (p>0.05). Taken together, the results demonstrated that cell viability can be improved by treatment with the ROCK inhibitor Y-27632, without losing iPSC stemness characteristics.

양성 유방 종양과 유방암 환자의 혈청 인지질 지방산 조성

심유진(Shim Eugene),안세현(Ahn Sei Hyun),황유정(Hwang You-Jeong),이양자(Lee Yang Cha) 韓國營養學會 2009 Journal of Nutrition and Health Vol.42 No.3

본 연구에서는 유방암과 유방 양성종양 환자의 혈청 인지질 지방산 조성을 대조군과 비교, 분석하였으며, 유방암 군을 병기에 따라 네 개의 군으로 분류하거나 림프절 전이여부에 따라 두 군으로 나누어 혈청 인지질 지방산 조성에 차이가 있는지를 조사하였다. 그 결과를 요약하면 아래와 같다. 1) 출산 자녀수에서 양성 유방 종양과 유방암 환자군이 대조군에 비하여 유의하게 적었다. 2) 대조군과 양성 유방 종양환자에 비해 유방암 환자에서 ω3계 PUFA인 혈청 인지질 DGLA와 AA의 수준이 높았으며 ω3계 PUFA인 eicosatrienoic acid는 대조군에 비하여 양성 유방종양군과 유방암군에서 낮았으며, DPA는 대조군에 비하여 유방암 환자에서 낮은 수준이었다. 3) 총 SFA의 합은 대조군과 양성 유방종양 환자에 비하여 유방암 환자에서 높은 것으로 나타났다. Palmitic acid는 대조군과 양성 유방 종양 환자군에 비하여 유방암 환자군에서 높았으나, stearic acid는 대조군에 비하여 양성 유방 종양 환자군과 유방암 환자군에서 낮은 것이 특징적이었다. 4) SI로서 C18:0/C18:1 비율은 대조군에 비하여 양성 유방 종양 환자군과 유방암 환자군에서 낮았다. 5) AA 수준은 0기에 비하여 Ⅰ, Ⅱ, Ⅲ기에서 높았으며, 림프절 전이 음성인 군과 양성인 군을 비교했을 때 양성인군에서 높은 것으로 나타났다. Stearic acid의 수준은 네 개의 군 중에서 가장 낮은 수준의 병기인 0기와 가장 높은 수준의 병기인 Ⅲ기 간에 유의한 차이가 있었으며 0기에 비하여 Ⅲ기에서 stearic acid가 낮은 것으로 나타났다. 이상에서와 같이 혈청 인지질의 지방산 조성은 유방암 환자군에서 변화되어 있었으며 특히 양성 유방종양 단계에서 변화가 나타난 지방산도 있었다. 또한 AA와 stearic acid 등 일부 개별 지방산들은 유방암의 진행 단계와도 관련되어 있었다. 따라서 혈청 인지질의 지방산 조성은 유방암의 예방 뿐 아니라 진행의 단계에 따라 유의 깊게 관찰해야 할 중요한 요소로 사료된다. Studies on the relationship between blood fatty acids and the risk of breast cancer have not yielded definite conclusions. The role of fatty acids in the development and progression of breast cancer is unclear. We conducted a case-control study to determine serum phospholipid fatty acid composition in benign breast tumor and breast cancer. Subjects consisted of 27 benign breast tumor and 68 breast cancer patients, and 28 matched controls. The levels of fatty acids were measured by gas chromatography. Higher arachidonic and palmitic acids were observed in breast cancer patients as compared with control and benign breast tumor patients. The percentage of total saturated fatty acids in breast cancer was higher than in control and benign breast tumor patients. The level of stearic acid was lower in benign breast tumor and breast cancer patients. Saturation index, the ratio of stearic to oleic acid, was lower in benign breast tumor and breast cancer patients compared to the control. Moreover, stearic acid was negatively and arachidonic acid was positively correlated with the cancer stage. In conclusion, our results support that serum phospholipid compositions of specific fatty acids are associated with the risk of benign breast tumor as well as breast cancer. Further studies are necessary to investigate mechanisms linked to the breast cancer etiology.

들숨근 훈련이 경수손상환자의 폐기능 향상에 미치는 영향

심유진(Yu-Jin Shim),문옥곤(Ok-Kon Moon),최완석(Wan-Suk Choi),김보경(Bo-Kyung Kim) 한국엔터테인먼트산업학회 2013 한국엔터테인먼트산업학회논문지 Vol.7 No.4

본 연구는 남자 경수손상환자들에게 들숨근 강화를 목적으로 한 호흡운동이 폐기능 향상에 미치는 영향을 알아보기 위해 실시하였다. 마비수준 C4-C6의 아급성, 만성 경수손상 환자 중 현재 흡연을 하지 않는 남자환자 17명이었으며, 주 3회, 총 6주간 들숨근 강화훈련을 실시하였다. 들숨근 훈련의 효과는 spirometer를 이용하여 측정하였고, 통계 분석은 대응표본 T검정을 실시하였으며 유의수준은 α=.05로 하였다. 연구 결과 아급성군의 PEF, FEV1, FVC, FEV1/FVC, Pi max, MV 등 모든 항목에서 운동 전후 유의한 증가를 보였다(p <.05, p <.01). 만성군은 PEF, FEV1, FEV1/FVC, Pi max, MV에서 유의한 증가(p <.05, p <.01)를 보였으나 FVC는 통계적으로 유의하지는 않았다. 본 연구를 통해 들숨근 강화훈련이 아급성 및 만성 경수손상환자의 폐기능 향상에 효과적임을 확인하였다. This research was carried out to find out the influence on the improvement of lung functions for male patients with cervical spinal cord injuries carrying out respiratory exercises with the purpose of strengthening inspiratory muscles. There were 17 currently-non-smoking male subjects who are patients with sub-acute or chronic injuries in the cervical spinal cords with paralysis levels between C4-C6, where they practiced the inspiratory muscle strengthening training for three times a week for six weeks in total. The effect of the inspiratory muscle training was measured using a spirometer, the statistical analysis was carried out using a matching sample of T-test, and the significance level was set at αα=.05. As a result of the study, significant increases (p <.05, p <.01) were observed after the exercise compared to before, for the sub-acute groupwith every item of: PEF, FEV1, FVC, FEV1/FVC, Pi max and MV. For the chronic group, statistically significant increases(p <.05, p <.01) were observed in: PEF, FEV1, FEV1/FVC, Pi max and MV, while the FVC did not show a statistically significant change. The study confirmed that the inspiratory muscle strengthening training is effective in improving the lung function for patients with sub-acute or chronic cervical spinal cord injuries.

암과 운동에 관한 문헌적 고찰

심유진(Shim, Yoo-Jin),정승삼(Jung, Seung-Sam),김아람(Kim, A-Ram),최승욱(Choi, Seung-Wook) 한국체육과학회 2013 한국체육과학회지 Vol.22 No.6

The purpose of this study is to understand effects of physical exercise program on improvements in mental and physical function and the quality of life through a review of the literature, to provide information about the importance and benefits of exercise for cancer patients, and in order to offer basic data for the development of an exercise program to meet the physical needs of each patient. By taking into consideration the review of the domestic and international literatures, the following results were obtained: First, positive effects of physical exercise program on physical function of cancer patients were reported. Since physical exercise can improve physical function, such as cardiovascular endurance, muscular strength, flexibility, and aerobic capacity, and reduced fatigue and improved vitality in cancer patients were observed. In addition, physical activity and exercise have effects on improvements in physical and mental capabilities; they reduce patient distress, anxiety, depression, stress, confusion, and mistrust associated with the emotional mental health improvement that can contribute to improve the quality of life of cancer patients.

연령대별 여성의 심혈관질환 위험요인 및 유산소성 능력 비교 분석

심유진(Shim, Yoo-Jin),김아람(Kim, A-Ram),김규태(Kim, Kyu-Tae) 한국체육과학회 2017 한국체육과학회지 Vol.26 No.4

This study aims to compare and analyze the body composition, cardiovascular diseases risk factors and aerobic function of adolescent and middle aged women in order to prevent cardiovascular disease of adolescent and middle aged women. As the result of comparative analysis on body composition, abdominal fat ratio of middle aged women was 0.1kg higher than adolescent women which showed significant difference(p〈.05). However, the skeletal muscle mass(kg), body fat(kg), lean body mass(kg), BMI(kg/m²), body fat percentage(%), and basal metabolism did not show significant difference. As the result of comparative analysis on branchial-ankle pulse wave velocity and ABI, the risk factors of cardiovascular diseases, middle aged women had about 290.42(s/cm) higher right branchial-ankle pulse wave velocity, which showed significant difference(p〈.05). Also, middle aged women had about 283.8(s/cm) higher the left branchial-ankle pulse wave velocity, which showed significant difference(p〈.05). As the result of comparative analysis on aerobic function, middle aged women had about 177,75(m) lower than adolescent women, and showed significant difference(p〈.05). Likewise, when compared to adolescent women, middle aged women are exposed to cardiovascular disease risk factors such as rapid changes in body composition or decrease in aerobic function. Thus, it requires women to take care of health and maintain the condition since their adolescence, and need particular attention to maintain and improve the aerobic function which poses positive influence of cardiovascular system.

운동프로그램이 보조적 항암화학요법을 받는 대장암 환자의 신체조성 및 스트레스에 미치는 영향

심유진(Shim, Yoo-Jin),최승욱(Choi, Seung-Wook) 한국체육과학회 2019 한국체육과학회지 Vol.28 No.4

The purpose of this study is to develop an exercise program for those colon cancer patients by applying an exercise program to those colon cancer patients who undergo adjuvant chemotherapy and thereby making a comparative analysis on the changes to their body composition and stress. The comparison of the body composition before and after the auxiliary anticancer chemotherapy showed that the exercise group registered a significant increase of about 1.25㎏ in the bone size and the muscle mass (p<.05) and the non-exercise group registered an insignificant increase of about 1㎏ (p>.05), while the body fat percentage decreased by about 2.25%, an insignificant difference in the exercise group (p>.05). It significantly increased by about 3.95% in the non-exercise group (p<.01). The muscle mass significantly increased by about 1.9㎏ in the exercise group (p<.05) and significantly by about 1.68㎏ in the non-exercise group as well (p<.05). The body fat percentage increased significantly by about 1.93㎏ in the exercise group (p<.05) and significantly increased by about 1.78㎏ in the non-exercise group as well (p<.05). Visceral fat increased significantly by about 8.32㎠ (p<.05) and increased significantly by about 22.45㎠ in the non-exercise group (p<.05). The comparison showed that the stress before and after the adjuvant chemotherapy decreased significantly by about 2.34 points (p<.05) whereas it did not register a significant difference in the non-exercise group even though it increased by about 2.5 points (p>.05). The current study confirmed that those cancer patients who underwent therapy experienced physically and mentally positive effects, which is expected to decrease the relapse rate and improve the quality of life for cancer patients.

운동이 위암환자에게 미치는 영향에 관한 문헌적 고찰

심유진(Shim, Yoo-Jin),김규태(Kim, Kyu-Tae) 한국체육과학회 2019 한국체육과학회지 Vol.28 No.6

This study aims to prove effects of exercise on stomach cancer patients through literature review and provide basic materials for development of programs of systematic exercise. In the study of Na et el.(2001) that carried out an aerobic exercise program oriented to stomach cancer patients after operation, NK cells increased in the exercise group. In the study of Choi J ini et al.(2012), according to the result of an 8-week-long exercise program oriented to stomach cancer patients under oral chemotherapy after operation, patients showed positive effects in terms of fatigue and anxiety, and improvement in NK cells and quality of life. On the other hand, the study of Sohn Yun-seon(2013) that carried out an exercise program in the complex anaerobic-aerobic form oriented to early-stage stomach cancer patients demonstrated improvement in maximal oxygen uptake. In the study of Wiebke Jansen et al.(2014), the results of an exercise program in the complex anaerobic-aerobic form oriented to gastrointestinal cancer patients under the palliative chemotherapy showed decreased level of fatigue of patients and increased sleeping hours and augmented level of muscular strength in the anaerobic exercise group. The studies confirmed that application of exercise programs for stomach cancer patients gave positive influences on them in all stages; after the operation, during the treatment and after the treatment. However, there are a small number of studies regarding development of customized exercise programs for stomach cancer patients. It is estimated that the number of stomach cancer patients will increase even more in the future because the rate of stomach cancer is steadily on the rise and the relative survival rate is also increasing after operation and treatment. It is considered highly important to provide exercise-based management for patients, during the post-diagnosis cancer treatment process and after treatment.