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신현근,박철우,이대섭,김우현,권우현 한국조명·전기설비학회 2012 한국조명·전기설비학회 학술대회논문집 Vol.2012 No.10
When harmonic pass the power system, it make some problems such as decrease capacity and increase heat of transformer etc. These problem can be caused destruction of distribution transformer that result in grand scale blackout. Therefore, this paper suggests a system to analyze of harmonic through distribution transformer in real time. The analyzer set is installed on distribution transformer and contribute to power supply safely.
한국 이유자돈에서 검출된 돼지 유행성 설사 바이러스의 유전자 서열 분석
신현근 ( Hyun Geun Shin ),김영훈 ( Yeon Hun Kim ),서태원 ( Tae Won Seo ),한정희 ( Jeong Hee Han ) 한국가축위생학회 2009 韓國家畜衛生學會誌 Vol.32 No.1
Porcine epidemic diarrhea virus (PEDV), an enveloped single stranded RNA virus in the family Coronaviridae, causes acute viral enteric disease in piglets. Recently outbreaks of porcine epidemic diarrhea (PED) have been rare in Europe but frequent in Asia. In Korea, the increase of PED prevalence is showing specially in postweaning pigs. The purpose of this study was to investigatenucleotide sequence of nucleocapsid protein gene of PEDV field isolates from postweaning pigs in Korea and get more information about the viruses. A total of 15 postweaing pigs clinically suspected of PEDV infection by severe watery diarrhea and dehydration were used in this study. Viral RNA was extracted from small intestines and stools of the pigs. The N gene was amplified by nested RT-PCR, purificated, sequenced, analyzed and then compared with published sequences of other PEDV strains. Three PEDVs were isolated from the suspected postweaning pigs. The N gene of three PEDV field isolates consisted of 483 nucleotides. These PEDV field isolates showed nucleotide sequence homology range from 99.6% to 95% with Chinese strains, from 99.8% to 95.2% with Korean strains, from 97.3% to 95.7% with Japanese strains and from 96.5% to 95.7% with Belgium and British strains. The encoded pritein shared range from 98.8% to 95.6% with Chinese strains, from 99.4% to 95% with Korean strains, from 97.5% to 96.3% with Japanese strains, from 95.6% to 95% with Belgium and British strains. By phylogenetic tree analysis based on nucleotide sequence, three PEDV field isolates were clustered into two groups which were Chinese isolate groups and other Korean isolate groups. These results indicated that some of PEDV field isolates prevailing in Korean postweaning pigs may be associated with those of Chinese strains and other Korean strains.