初等敎員 個人中心 自律硏修 實態調査

辛在玉 건국대학교 교육대학원 1995 敎育論叢 Vol.23 No.-

심장근육모세포주에서 Adriamycin으로 유발된 세포 사멸에 대한 Cardiotrophin-1의 효과

신재옥,주은선,송현미,윤수현,임병관,최진호,김덕경,전은석 대한심장학회 2008 Korean Circulation Journal Vol.38 No.5

Background and Objectives: Adriamycin (doxorubicin, ADR) is a highly effective anti-neoplastic drug, but its clinical use is limited by its adverse side effects on the heart. Cardiotrophin (CT-1), a potent cardiac survival factor, is capable of inhibiting apoptosis in cardiac myocytes. The aim of this study was to investigate the cytoprotective effects of CT-1 against ADR-induced apoptosis in vitro. Materials and Methods: We determined a reasonable ADR concentration for inducing cell death by utilizing a cell survival test performed in a dosedependent manner. To determine the requirements for apoptosis in ADR-treated cardiac myocytes (H9c2 cells), we examined the effect of CT-1 on survival and apoptotic changes using a cell counting kit (CCK), RT-PCR, and Western blotting. Results: In analyzing cell survival as determined by CCK, ADR-induced cell death was found to occur in a dose-dependent manner (50% death at 24 hours after 2 μM of ADR), and ADR was shown to decrease procaspase-3. On RT-PCR, expression of Bax-α mRNA increased and Bcl-2 decreased during the 24 hours after ADR treatment. Consequently, the ratio of Bax-α/Bcl-2 mRNA peaked at 24 hours after ADR treatment. In contrast, CT-1 effectively attenuated the ADR-induced cell death in a dose-dependent manner. The changes in Bax-α and Bcl-2 mRNA expression after ADR treatment were reversed by CT-1 (1 ng/mL) treatment. The protein levels of procaspase-3 decreased after ADR treatment, an effect which was reversed by CT-1 treatment. Akt phosphorylation was also increased by CT-1, demonstrating that CT-1 inhibited apoptosis induced by ADR. Conclusion: These data demonstrated that ADR-induced apoptosis of cardiomyocytes can be prevented by CT-1; therefore, it may be possible to use CT-1 as a cardioprotective agent during ADR chemotherapy in patients with cancer. Background and Objectives: Adriamycin (doxorubicin, ADR) is a highly effective anti-neoplastic drug, but its clinical use is limited by its adverse side effects on the heart. Cardiotrophin (CT-1), a potent cardiac survival factor, is capable of inhibiting apoptosis in cardiac myocytes. The aim of this study was to investigate the cytoprotective effects of CT-1 against ADR-induced apoptosis in vitro. Materials and Methods: We determined a reasonable ADR concentration for inducing cell death by utilizing a cell survival test performed in a dosedependent manner. To determine the requirements for apoptosis in ADR-treated cardiac myocytes (H9c2 cells), we examined the effect of CT-1 on survival and apoptotic changes using a cell counting kit (CCK), RT-PCR, and Western blotting. Results: In analyzing cell survival as determined by CCK, ADR-induced cell death was found to occur in a dose-dependent manner (50% death at 24 hours after 2 μM of ADR), and ADR was shown to decrease procaspase-3. On RT-PCR, expression of Bax-α mRNA increased and Bcl-2 decreased during the 24 hours after ADR treatment. Consequently, the ratio of Bax-α/Bcl-2 mRNA peaked at 24 hours after ADR treatment. In contrast, CT-1 effectively attenuated the ADR-induced cell death in a dose-dependent manner. The changes in Bax-α and Bcl-2 mRNA expression after ADR treatment were reversed by CT-1 (1 ng/mL) treatment. The protein levels of procaspase-3 decreased after ADR treatment, an effect which was reversed by CT-1 treatment. Akt phosphorylation was also increased by CT-1, demonstrating that CT-1 inhibited apoptosis induced by ADR. Conclusion: These data demonstrated that ADR-induced apoptosis of cardiomyocytes can be prevented by CT-1; therefore, it may be possible to use CT-1 as a cardioprotective agent during ADR chemotherapy in patients with cancer.

全子宮腔內 癒着에 起因된 外傷性 子宮性 無月經 : Asherman's Syndrome

申再玉 大韓産婦人科學會 1965 大韓産婦人科學會雜誌 Vol.8 No.8

正常마우스 卵巢組織 生後發育에 關한 自記放射法的 硏究

申再玉,羅福瑛 고려대학교 의과대학 1974 고려대 의대 잡지 Vol.11 No.1

Postaatal growth and aging effect on the cell renewal system of ovary were observed by means of microradioautography. The 39 female mice were divided into 3 age groups, namely suckling young, young adult and old adult. The age ranges from 4-day to more than 50-week. The mice were treated with a single dose of H³-thymidine, 0.8 microcurie per gram ef body weight, intraperitoneally. The tissues were removed 2-hour after the treatment, and were examined histologically and labeling index were calculated after the necessary procedures. 1. Cell renewal of the ovary showed a generally high rate throughout the various postnatal ages. The highest labeling was observed in 4-day old group and thereafter gradual downcast was seen during suckling stage. At the young adult, the radioactive indices reached nearly maximal value when it was estrus. On the contrary, old adult showed lowest cell renewing during diestrus. 2. The ovarian cycle has much to do with the renewal patterns of all the cellular elements and quite an active patters was demonstrated during estrus, except in connective tissue. 3. All the connective tissue element appeared scantily in the younger age and gradually increased their amount with age proceed. At the old adult, many of the structures were replaced by abundant blood vessels and connective tissue. 4. The primordial follicle and the primary follicle should be considered separately as a different unit under the histological base. 5. None of the labeling on the oocytes was noted throughout this observation.

실리콘 산화공정에 대한 실험적 연구

신재옥,구경완,홍봉식 충남대학교 공업교육연구소 1986 論文集 Vol.8 No.3

Dry Oxidation and Wet Oxidation process of (100) P-type silicon have been examined experimentally. The oxidation temperature was 1,100℃ and Oxygen flow rate was changed from 200 cc/min to 1000 cc/min. From the experimental measurements, moxid₄atmon temperature, time and Oxygen flow rate have been graphed for oxide layer 0.24 μ∼0.9 μ in thickness. The optimum value of O₂ flow rate has been found to be 1.4 cm/sec. The quality of the grown oxide layer has been investigated in terms of the dielectric constant, breakdown voltage, fixed surface charge density(Ωf), and mobile ion charge density(Ωm/g). From these measurements, it is concluded that the quality of the oxide layer is sufficient to expect the normal operation of MOS transistors.

正常마우스 卵巢組織 生後發育에 關한 自記放射法的 硏究

申再玉 고려대학교 의과대학 1974 고려대학교 의과대학 잡지 Vol.11 No.1

한국부인(임신말기임부)의 X선골반 계측치에 관하여

신재옥 ( JO Shin ) 대한산부인과학회 1967 Obstetrics & Gynecology Science Vol.10 No.4

N/A X-ray pelimetry by Colcher-Sussman method in 80 primigravids within 2 weeks from E.D.C., who were admitted for delivery since June 1964 till June 1966, was taken. The measurements of each diameter at three pelvic were as following; 1)Pelvic inlet M+-δcm. i)Anatomical true conjugate 11.82+-1.02 ii)Obstetrical ture conjugate 11.34+-1.03 iii)A-P diamter of actual inlet 12.17+-0.96 iv)Transverse diamter 12.60+-0.75 2)Mid-pelvis i)Interspinous diamter 10.12+-0.98 ii)A-P diamter 11.43+-0.82 3)Pelvic outlet i)Intertuberous diameter 9.99+-0.89 ii)Posterior sagittal diameter 7.26+-0.92

전자궁강내 유착에 기인된 외상성 자궁성 무월경

신재옥(JO Shin) 대한산부인과학회 1965 Obstetrics & Gynecology Science Vol.8 No.8

There cases of traumatic intrauterine adhesions (Asherman`s syndrome) which developed at an interval of 10 months to 6 years after curettage were studied. Amenorrhea and sterility occurred in all the cases. And its definition, incidence, etiology, pathology, pathologic anatomy, symptoms, diagnosis, treatment, prognosis, and prevention are reviewed briefly.

제방형 웨어의 마루길이 및 유량변화에 따른 수리특성의 실험적 해석

권선도,신재옥,김희수,송부호,이종석 한국수자원학회 2001 한국수자원학회 학술대회 Vol.2001 No.1

非·接觸性 流速計를 利用한 水深平均流速 換算係數의 決定

이종석,愼在玉,金喜洙,姜泰印,金光範 한밭대학교 건설안전기술연구소 2001 건설안전기술논문집 Vol.1 No.-

This study is determined a optimum conditions of apparatus operations and estimated a conversion factor of depth-averaged velocity (DAVCF) in puting a DAVCF to practical use through the field tests of a electromagnetic wave surface velocimeter (EWSVM). As a result the efficiency of a EWSVM show excellent in comparison with water-submerged current meters for a peculiarity of mechanical and a capacity at field tests. It show in case of a facile operations of vertical angle and declination as well as a convenience of measured method at performance tests. Even though field test are three stations of restricted conditions, the DAVCF values showed 0.472∼0.663, 0.732∼0.869, and 0.736∼1.047 for variations of vertical angle when velocity and bed material conditions are lower of gravel, medium of clay, and higher of gravel, respectively. Those ranges of flow velocity conditions are 0.3∼0.5㎧, 0.5∼1.3㎧ and 1.3∼1.5㎧ for lower, medium and higher in river channel, respectively. And the mean value of a DAVCF showed 0.757 for three conditions, but excluding condition of lower velocity, it showed 0.833, and only it showed 0.875 for the higher velocity condition.