Nuclear factor I-C가 치근발생 과정에서 Hertwig's 상피초 형성에 미치는 영향

신인철,박주철,정문진,오현주,박선화,이창섭,김흥중,Shin, In-Cheol,Park, Joo-Cheol,Jeong, Moon-Jin,Oh, Hyun-Ju,Park, Sun-Hwa,Lee, Chang-Seop,Kim, Heung-Joong 대한소아치과학회 2005 大韓小兒齒科學會誌 Vol.32 No.3

치아의 형성은 상피-간엽간의 상호작용을 통해 조절되어지는 복잡한 발생과정이다. 지금까지 치관의 발생에 관여하는 유전자 및 그들의 신호전달경로에 관한 연구는 다수 진행되어 왔지만 치근의 발생을 조절하는 기전에 대해서는 별로 알려진 것이 없다. 최근에 NFI-C knock out 생쥐에서 정상치관에 비정상적인 치근을 가지는 치아가 보고되었다. 본 연구의 목적은 NFI-C가 어떻게 치근의 형태와 상아모세포의 분화에 관여하는지를 규명하는 것이다. NFI-C knock out 생쥐의 치근 발생동안에 HERS의 역할을 연구하고자 cytokeratin 면역조직화학적방법과 치근상아질의 특성을 규명하기 위해 DSPP mRNA in-situ hybrydization법을 수행하였다. 1. NFI-C knock out 생쥐의 치근형성시 HERS의 역할 Wild type과 knock out type 모두에서 cytokeratin은 모든 HERS 세포들과 반응하였고, HERS와 법랑상피 사이의 양성반응세포들의 연속성은 치경부 부위에서 소실되었다. Knock out type에서 치근상아질이 침착된 후, cytokeratin 양성-HERS 세포들은 치경부에서 불규칙한 배열과 극성의 상실을 보였다. 2. NFI-C knock out 생쥐의 치근상아질의 특성 DSPP mRNA의 발현은 wild type에서 치관과 치근상아질의 상아모세포 모두에서 강한 발현을 보인 반면, knock out type에서는 치관부위 상아질의 상아모세포에서만 강한 발현을 보였다. 3. NFI-C knock out 생쥐의 치근 발생과정에서 HERS는 치관으로부터 정상적인 확장을 보인 반면, 치근부위에서의 상아 모세포 분화는 실패하였다. 위의 결과들로 보아 NFI-C는 치근형성 과정에서 상아모세포 분화에 중요한 역할을 하는 것으로 사료된다. Tooth formation is a complex developmental process that is mediated through a series of reciprocal epithelial-mesenchymal interactions. Several signal pathways and transcription factors have been implicated in regulating molar crown development, but relatively little is known about the regulation of root development. It was reported that NFI-C knockout mice showed abnormal root formation with normal crown. The aims of this study are to elucidate how the NFI-C regulate the determine of root shape and odontoblasts differentiation. We carried out immunohistochemistry using cytokeratin to investigate the role of Hertwig's epithelial root sheath and DSPP mRNA in-situ hybridization to conform the nature of root dentin during root development in NFI-C knockout mice. Cytokeratin reacted with all the HERS cells and the continuity of cytokeratin positive cells between the HERS cells and enamel epithelium was lost in the cervical region both wild and K/O types. After root dentin deposition cytokeratin positive-HERS cells showed irregularity and loss of polarity in the cervical region in K/O type. DSPP mRNA was strongly expressed in odontoblasts of crown and root dentin in wild type mice, whereas expression of DSPP mRNA was restricted in odontoblast of crown dentin in the K/O type. During root formation in NFI-C knockout mice, HERS normally grow out of the crown but fail to induce odontoblast differentiation in root portion. These results suggest that NFI-C may play important roles in odontoblast differentiation during root dentin formation.

Glycerol이 흰쥐 신장에서의 Malondialdehyde 함량과 Superoxide Dismutase 활성도 및 요중 단백질 배설량과 N-acetyl-β-D-glucosaminidase 활성도에 미치는 영향

신인철(In Chul Shin),고현철(Hyun Chul Koh) 대한약리학회 1996 대한약리학잡지 Vol.32 No.2

In an attempt to dofine the early biochemical determinants that participate in the pathogenesis of glycerol-induced nephrotoxicity, especially focusing on oxygen free radicals and N-acetyl-β-D-glucosaminidase(NAG) activity, we studied 24-hours urine outflow, 24-hours urinary protein excretion and urinary NAG activity after the injection of glycerol and also we studied malondialdehyde(MDA) level and superoxide dismutase(SOD) activity in the kidney of rats at 24hr after the injection of glycerol. Sprague-Dawley albino rats weighing 240 to 260 gm were injected intramuscularly with a 50% solution of glycerol(2ml/kg, 4ml/kg and 8ml/kg). The group treated with glycerol showed significantly lower urine outflow level and urinary protein excretion level and higher urinary NAG activity after the injection as compared to those of control group. Also the group treated with glycerol showed significantly higher MDA level and lower SOD activity at 24hr after the injection as compared to those of control group. These results suggest that the excessive oxygen free radicals resulting from the depression of SOD activity is an important determinant in the pathogenesis of glycerol-induced nephrotoxicity and higher urinary NAG activity is an index of renal tubular cell damage in the glycerol-induced nephrotoxicity.

SDN을 통한 스마트그리드 복원력(Resilience) 향상 이슈

신인철(Incheol Shin) 한국정보보호학회 2015 情報保護學會誌 Vol.25 No.5

SDN (Software Defined Networking)은 동적 재설정(Dynamic Reconfiguration)기능을 통해 지금껏 존재하지 않았던 유연성(Flexibility)을 IP(Internet Protocol)에 제공한다. 또한, 네트워크 관리, QoS (Quality of Service) 최적화, 시스템 복원력(Resilience) 강화를 위한 다양한 응용프로그램을 지원한다. 스마트그리드(Smart Grid)시스템에 SDN을 적용하기 위한 다양한 연구가 진행 중이며, 본 문서에서는 다양한 사고(Failures) 혹은 불법적인 공격으로부터 해당 시스템 복원력향상을 위한 이슈에 대해 언급한다. 이와 같은 문제점들에 대한 논의 없이 전력회사는 SDN의 장점을 충분히 활용하지 못할 가능성이 높다. 본 문서를 통해, SDN을 통한 스마트그리드 복원력향상, SDN으로 인한 추가적인 보안위협 등에 대해 논의할 것이다.

마태공동체의 포괄적 성격을 통하여 본 마태복음의 구속사

신인철(In-Cheol Shin) 한국신약학회 2007 신약논단 Vol.14 No.4

권면의 관점에서 본 데살로니가 교회박해자(살전 2:13-16)

신인철(Shin, In-Cheol) 한국신약학회 2013 신약논단 Vol.20 No.3

살전 2:13-16은 데살로니가 교회의 박해 상황을 이해하는 데 중요한 역할을 한다. 이 단락에 대한 주된 논쟁 가운데 하나는 데살로니가 교회를 박해한 주체가 누구인지를 밝히는 것이다. 학자들은 데살로니가 교회를 박해한 주체를 디아스포라 유대인이거나 아니면 이방인으로 본다. 본 논문은 살전 2:13-16을 권면의 관점에서 해석함으로 데살로니가 교회를 박해한 주체가 누구인지를 드러내는 것이다. 이 가설을 증명하기 위해 먼저 살전 2:13-16의 역사성을 다룰 것이다. 그리고 데살로니가 교회의 박해 상황을 살펴볼 것이다. 마지막으로 바울이 사용한 권면의 유형을 살펴보고, 이러한 권면의 유형이 살전 2:13-16에 어떻게 적용되었는지를 통해 데살로니가 교회를 박해한 주체가 누구인지를 확인할 것이다. 1 Thessalonians 2:13-16 is the core to understand the persecution of the Thessalonians church. One of the main debates that has been going on for a long time in this text is who is the persecutor of the Thessalonians Church. This discussion made two views. One side confirmed that the Thessalonians church was persecuted by the diaspora Jewish. The other side considers the church was persecuted by gentiles who were living in Thessalonians as a neighbor of the church. However, the assumption of this articles is that the gentiles were the persecutors of the Church. Thus, the assumption of this article will be proven in three ways, First, it is considered that the text is a genuine letter of Paul. Some scholars denied that the text is Paul’s own writing. If the text is not written by Paul, it is not Paul’s exhortation because the main focus of this article is to look at the persecution of the Thessalonians church in a exhortation perspective. The second way is the situation of the persecution of the Thessalonians church. It depends on whether the Jewish community in the Synagogue or Gentiles residing in Thessalonians was connected. It shows us that after Paul left Thessalonia, Jewish persecution against the Christian community in Thessalonians slowly stopped. However, Gentile persecution rose against the Thessalonians church. The Gentiles persecuted the Church because they were afraid of the growing number of Christians and because the Christians did not cooperate with their city policy of religion and social culture. Third, who the persecutor of Thessalonians church was will be analyzed through the exhortation perspective. Especially, the method of Paul’s exhortation model was imitation and recollection. Therefore, the purpose of telling the suffering Thessalonians church about how God’s church in Judea suffered from the Jews is to exhort the Thessalonians church to overcome like God’s church. The contribution of this article is how it analyzed and confirmed that the Thessalonians Church was persecuted by the Gentiles through the exhortation perspective.

호텔기업의 업무스킬교육이 경력개발 및 업무성과에 미치는 영향에 관한 연구

신인철 ( Inchul Shin ),김형일 ( Hyeong Kim ) 한국호텔리조트학회(구 한국호텔리조트카지노산학학회) 2015 호텔리조트연구 Vol.14 No.4

The hotel industry required the high levels of the human resources because of the employees faces customers in every work point. Therefore, for obtaining the high quality of service the skill education could be an one of the most important factors. This study is about the relationship between career development, organizational achievement, personal achievement by education. The survey was from 1, August to 15, August in 2015 within hotel employees who works in the five star hotels in Seoul and Incheon. The study used total 258 for final analysis and applied SPSS 18.0 for technique statistics analysis, frequency analysis, factor analysis, reliability analysis, correlation analysis and regression analysis. According to result the work skill education effected on career development, organizational development and personal development. Also, the career development effected on the organizational development. Therefore, the employee``s education is very significant in the hotel organizations for deliver the high quality of service.

염화 제 2 수은이 흰쥐 간장에서의 지질 과산화와 Oxygen Free Radical 제거 효소 활성도에 미치는 영향

신인철(In Chul Shin),고현철(Hyun Chul Koh) 한국응용약물학회 1994 Biomolecules & Therapeutics(구 응용약물학회지) Vol.2 No.3

Wistar albino rats were injected subcutaneously with mercuric chloride (5mg/kg) to define the early biochemical determinants that participate in the pathogenesis of mercuric chloride-induced hepatotoxicity, especially focusing on oxygen free radicals, we studied malondialdehyde(MDA) level and the activities of catalase and superoxide dismutase in the liver of rats at 24, 48 and 72 hr after the injection of mercuric chloride. MDA levels at 24, 48 and 72 hr after the injection of mercuric chloride increased as compared with that of control group. The activities of catalase and superoxide dismutase at 24, 48 and 72hr after the injection of mercuric chloride decreased as compared with that of control group. These results suggest that the depression of the activities of catalase and superoxide dismutase resulting from excessive oxygen free radicals is an important determinant in pathogenesis of mercuric chloride-induced hepatotoxicity.

산겨릅나무 추출물의 이화학적 특성과 암세포 성장 억제 효과

신인철 ( In Cheol Shin ),사재훈 ( Jae Hoon Sa ),심태흠 ( Tae Heum Shim ),이진하 ( Jin Ha Lee ) 한국응용생명화학회 2006 Applied Biological Chemistry (Appl Biol Chem) Vol.49 No.4

백부장의 하인 치유기사에 함의된 마태공동체의 이방인 선교(마 8:5-13)

신인철(Shin, In-Cheol) 침례신학대학교 출판부 2019 복음과 실천 Vol.64 No.1

트랜스포머 기반 압타머-단백질 상호작용 예측 분류 모델 설계

신인철(IN CHEOL SHIN),최정훈(JEONG HOON CHOI),김태영(TAE YOUNG KIM),송인혁(IN HYUK SONG),손동현(DONG HYUN SON),유승태(SEUNG TAE YOO),송길태(GIL TAE SONG) 한국정보과학회 2021 한국정보과학회 학술발표논문집 Vol.2021 No.12