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한국 Streptomyces sp. 로 부터 분리한 방향족 화합물과 지질 화합물의 세포독성 연구
신석우(Suck Woo Shin),염곤(Kon Ryeom) 한국응용약물학회 1997 Biomolecules & Therapeutics(구 응용약물학회지) Vol.5 No.2
In an effort to screen new selective antitumor agents from the broth of soil microorganism, cytotoxicity oriented screening was performed against tumor cells and 3 compounds (Compound 1, 2 and 3) were isolated from Streptomyces parvullus ISP 5048 and their chemical structures were determined. Among these compounds, Compound 2 showed the highest cytotoxicity against P388D1 and L1210. While the IC_(50) values of compound 2 against P388D1 and L1210 were 0.073 ㎍/㎖ and 0.07 ㎍/㎖, respectively, and the IC_(50) value of Compound 3 was 0.17 ㎍/㎖ against human lung cancer cells, A549, the cytotoxicity of Compound 2 and 3 against normal cell line, Vero E6 cell was about 4- and 8-fold lower than that of adriamycin. Based on the chemical analysis data, Compound 3 was octacosamicine A, a known antibiotic, which was reported by Dobasih et al. (1988). Taken together the results demonstrated that Compound 2 and Compound 3 has the possibility to be developed as antitumor agent because of its potent cytotoxicity as well as high selectivity against various cancer cell lines.
Streptomyces 속 야생균주들이 생산하는 세포독성물질과 plasmid 와의 연관성에 관한 연구
김미용(Mi Yong Kim),신석우(Suck Woo Shin),최병돈(Boung Don Choi),염곤(Kon Ryeom) 한국응용약물학회 1995 Biomolecules & Therapeutics(구 응용약물학회지) Vol.3 No.2
We isolated Streptomyces spp. from Korean soil, which showed high cytotoxicity against tumor cell lines, L1210 and P388D1. Among 30 strains, three strains (DKM 104, DKM 128, DKM 409) were appeared to possess plasmid. Strain DKM 104 and DKM 128 had two CCC(Covalently Closed Circular) form plasmid, about 20 Kb in size and about 1 Kb compared with λ Hind III DNA size marker. And strain DKM 409 had three plasmids, among which two plasmid were CCC form about 1 Kb and about 20 Kb compared with same size marker. To find out whether plasmid involved in production of antitumor agent or not, we performed to curing experiment. Comparing cytotoxicity between culture filtrate of plasmid-containing strains and cured strains, we knew that only the cytotoxic activity of the strain DKM 128 was involved in plasmid.
항암성 생약제로 부터 항암성분의 추출 및 항암활성 측정
이영기,신석우,염곤 단국대학교 신소재기술연구소 1993 신소재 Vol.3 No.-
암치료에 많이 사용되는 생약제 16종을 대상으로 항암활성을 규명하고자 in vitro에서 세포 독성능을 측정하여 다음과 같은 결과를 얻었다. HEP 2 cell에 대해 세포 독성능을 높게보인 생약제는 하고초>고삼>화누>선복화 순이었고, HeLa cell에 대해 세포독성능을 높게 보인 생약제는 선복화>아출>포공영>화누>구맥>하고초>고삼>오공>금은화>천문동 순 이었다. RAW 71 cell에 대해 세포독성능을 높게 보인 생약제는 구맥>화누>고삼>호모초>천문동 순 이었고, VERO E6 cell에 대해 세포 독성능을 높게 보인 생약제는 선복화>하고초 순 이었다. 또한 Herbal drug 처리에 의한 HEP 2와 HeLa 세포의 세포 독성능과 Staphylococcus aureus의 항균력간의 관계를 알아본 결과 HEP 2 cell은 약 43%, HeLa cell은 약 71%가 일치하였다. We tested the cytotoxic ability on the tumor cell line by 16 kinds of herbal drugs of which contains the alkaloids and flavoids which shows high antitumoral activity. This has been done on the condition of in vitro. the conclusion are as follows. 1. The herbal drugs which have shown the strong cytotoxic activity to HEP 2 cell are as follows. Prunella vulgaris has the strongest activity, followed by Sophorra angustifolia>Trichoosanthes kirilowii>Inula brontannaca. 2. The herbal drugs which have shown the strong cytotoxic activity to HeLa cell are as follows. Inula britannica has the strongest activity, followed by Curcuma zedoooaria>Tarazacum platycarpum>Trichosanthes kirilowii>Dianthus sinesis>Prunella vulgaris>Sophra angustifolia>Scolopendra subspinpes>Lonicera Japonica>Asparagus cochinensis. 3. the herbal drugs which have shown the strong cytotoxic activity to RAs 71 cell are as follows. Dianthus sinesis has the strongest activity, followed by Trichosanthes kirilowii>Sophra angustifolia>Corispermum stauntoni>Asparagus cochinensis 4. The herbal drugs which have shown the strong cytotoxic activity to VERO E6 cell are as follows. Inula britannica has the strongest activity, followd by Prunella vulgaris. 5. After the treatment with herbal drugs, the relationshipbetween the cytotoxic activity to HEP 2 and HeLa cell, the antimicrobial activity of Staphylococcus aureus was found. these two activites show the similarity of about 43% in HEP 2 cell and 71% in HeLa cell.