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박준구,최병돈,김승철,염곤,Park, Joon-Koo,Choi, Boung-Don,Kim, Seung-Chul,Ryeom, Kon 환경독성보건학회 1993 환경독성보건학회지 Vol.8 No.3
An Actinomycete strain isolated from Mt. Dea-Dun had a strong antifungal activity. The culture brith produced by isolated strain showed only antifungal activity against fungi with the exception of yeast and bacteria. It was heat stable, dissolved in ehtylacetate. The concentrated antifungal agent showed cytotoxicity against HEP-2 and HeLa as tumor cell line, and showed weak cytotoxicity against VERO 36 as normal cell line. Morphological and physiological characteristics were tested with isolated strain. The spore color of isolated strain was gray. It had a short chain and produced brown colored lytic substance in yeast extract-malt agar. The cell wall of isolated strain was composed of meso-DAP, and we suggested it as genus Actinomadura. In the existing of chemical inhibitor, isolated strain grew on the condition of 0.0001% crystal violet, 0.1% phenol, 0.01% sodium azide and 10% sodium chloride. Carbon utilization of isolated strain was shown that glucose, sucrose, manitol and sodium citrate were well utilized.
Streptomyces 속 야생균주들이 생산하는 세포독성물질과 plasmid 와의 연관성에 관한 연구
김미용(Mi Yong Kim),신석우(Suck Woo Shin),최병돈(Boung Don Choi),염곤(Kon Ryeom) 한국응용약물학회 1995 Biomolecules & Therapeutics(구 응용약물학회지) Vol.3 No.2
We isolated Streptomyces spp. from Korean soil, which showed high cytotoxicity against tumor cell lines, L1210 and P388D1. Among 30 strains, three strains (DKM 104, DKM 128, DKM 409) were appeared to possess plasmid. Strain DKM 104 and DKM 128 had two CCC(Covalently Closed Circular) form plasmid, about 20 Kb in size and about 1 Kb compared with λ Hind III DNA size marker. And strain DKM 409 had three plasmids, among which two plasmid were CCC form about 1 Kb and about 20 Kb compared with same size marker. To find out whether plasmid involved in production of antitumor agent or not, we performed to curing experiment. Comparing cytotoxicity between culture filtrate of plasmid-containing strains and cured strains, we knew that only the cytotoxic activity of the strain DKM 128 was involved in plasmid.
최병돈,김승철,염곤 단국대학교 대학원 1992 學術論叢 Vol.16 No.-
A wild strain was isolated through screening method from soil sample and identified by ISP system. This strain was shown to be similar to Streptomyces noboritoensis. The antibiotic which produced by wild strain showed only activity against the gram(+) bacteria. The antibiotic was purified by butanol extraction, column chromatography and paper chromatography. This antibiotic was soluble in alcohols and was colored by ferric chloride, anisaldehyde-sulfuric acid, acetic anhydride-sulfuric acid and antimony(Ⅲ) chloride. The chemical structure through color reaction. UV spectrum, IR spectrum, mass spectrum was assumed as a terpene being alcoholic hydroxyl group and the molecular weight was 211.