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廉곤,李長勳 한국환경독성학회 1986 환경독성보건학회지 Vol.1 No.1
The determination of fungal flora in some kinds of cereals have been carried out in other to obtain an appropriate information of the population of fungi and toxin productivity. The results were summarized as follow; 1. The predominant genera were Aspergillus, Penicillium, Mucor, Rhizopus, Alternaria, Fusarium. 2. Six of Aspergillus flavus were aflatoxin-producing strains. 3. Sample barleys were found to contain the highest content of aflatoxin. 4. In electron microscopic studies of liver cells from mouse which had been injected with crude toxin, the liver cells showed the cytoplasmic change.
患者로부터 分離同定한 病原性 大腸菌의 性狀에 관한 硏究
염곤,劉承兆,李善煥 成均館大學校 科學技術硏究所 1984 論文集 Vol.35 No.1
Strains of Pathogenic Escherichia coli isolated from the diarrheal patients in the Seoul were 9 serotypes that were documented previously in the international literature as associated with Pathogenic E. coli. Of 13 strains, there are 9 serotypes and 3 strains are Enterotoxigenic E. coli. The serotypes of Enterotoxigenic E. coli were 06 : K15, 0148 : K, 025 : Kl. In order to detect the heat-stable enterotoxin of Enterotoxigenic E. coli, supernants of CA-YE culture were administered into the intestines of suckling mice and fluid accumulation in the intestine were measured after 4 hrs., by weighing. In the results of suckling mice test, it have proved that 2 strains (06 : K15, 00148 : K) had produced positive tests. In the results of the antibiotics susceptibility test, Nitrofurantion and Nalidixic acid about the urinary tract infection have susceptibility but Tetracyclines such as Doxycycline, Oxytetracycline have high resistance. Although Neomycin had been treated skin or wounds, generally it had resistance because we suggest that it have cross-resistance with kanamycin which is similar activity and widely treated the disease in the pathogenic E. coli.
염곤,劉承兆,李長勳 成均館大學校 科學技術硏究所 1985 論文集 Vol.36 No.1
The pathogenesis of gastrointestinal infections caused by E. coli has been attributed to the ablility of some strains to produce enterotoxins or to invade the intestinal mucosa directly. Enterotoxigenic strains may produce either a heat-labile toxin(LT), a heat-stable toxin(ST) or both LT and ST. The heat-labile enterotoxin of E. coli causes a diarrhea with a delayed onset and similalar to cholera toxin in its mode of action and immunogenicity. In contrast to LT, ST is of low molecular weight, nonimmunogenic, and not neutralized by cholera or LT antitoxin. The infant mouse test was used for the detection of ST and EnzymeL-inked Immunosorbent Assay was used to dettect LT, and serological test was used to detect Enteropathogenic E. coli and Enteroinva sive E. coli. And biochemical examinations were done. 1. Strains of pathogenic E. coli isolated from the diarrheal patients in Seoul were identifided in 68 (21.3%) of 319 cases. 2. Stranis of enterotoxigenic E. coli was detcetected in 56 cases; 49 were positive for heat-labile enterotoxin and 1 was positive for only the heat-stable enterotoxin. 3. Strains of enteropathogenic E. coli was detected in 10 cases. 4. The serotypes of enteropathogenic E. coli were 026 : K60, 055 : K59, 0111 : K58, 0119 : K69, 0126 K71, 0128 : K67, 01 : K51. 5. D-mannitol, lactose, maltose were used for growth in toxigenic E. coli. Malonate utilization, urease activity, gelatin liquefaction were negetive in enteropathogenic E. coli, enterotoxigenic E. coli and enteroinvasive E. coli.
Enzyme Linked Immunosorbent Assay를 이용한 진엽독소 검출에 관한 연구
염곤,유승북,이장훈,Ryeom, K.,Yu, S.J.,Lee, J.H. 환경독성보건학회 1990 환경독성보건학회지 Vol.5 No.3
Aflatoxins, produced by strains of Aspergillus flavus and Aspergillus parasiticus, can be found worldwide in corn, barley, peanuts, and other commodities. Among this group of toxins, aflatoxin B$_1$was realized to be one of the most potent environmental carcinogens, mutagens and teratogens. It is routinely monitored by methods such as thin layer chromatography, liquid chromatography, fluorodensitometric technique and radioimmunoassay. However, these assays are expensive, necessitate radioactive reagents, and require overnight incubation. In this study, the determination of fungal flora in several sorts cereals has been carried out in order to obtain an appropriate information of the population of fungi. The quantitative analysis of aflatoxin B$_1$has been carried out by High Performance Liquid Chromatography (HPLC) method and Enzyme Linked Immunosorbent Assay (ELISA). The results were summarized as follow: 1) From the 100 samples,313 colonies of fungi were isolated. Among the 313 colonies, 274 were possible to identify into 11 genera. The identified genera were Aspergillus Penicillium, Mucor, Rhizopus, Alternaria, Cladosorium, Fusarium, Circinella, Chrysosporium, Paecilomyces and Phoma. 2) Six of Aspergillus flavus were aflatoxin-producing strains. Aspergillus flavus isolated from sample barleys was contained the highest content (21.8 $\mu\textrm{g}$/ml) of aflatoxin B$_1$. 3) The yield of aflatoxin B$_1$-oxime compound was appromately 75%. Aflatoxin B$_1$-oxime-Human serum albumin was approved by formal consent as complete antigen. 4) Direct competitive ELISA permitted detection of 0.15 ng levels. In the quantitative microanalysis, ELISA was superior to HPLC method.
염곤,최병돈,Ryeom, Kon,Choi, Byung-Don 한국생약학회 2000 생약학회지 Vol.31 No.1
These studies were designed to determine the potential cytotoxic activity of MeOH extracts of 65 crude drugs against leukemia L1210 and $P388D_1$, cell line in vitro, of which 25 samples were selected. The n-BuOH extracts of 25 samples were measured using the same method and nine were selected. These samples were measured for the potential antitumor activity against $P388D_1$, for life span in vivo, and against sarcoma 180 for tumor weight. On the basis of results, Notoginseng Radix, Anemarrhenae Rhizoma, Albizziae Cortex, Portulacae Herba, Bupleuri Radix were evaluated the effective plant on the antitumor activity. In addition, the mixture of Notoginseng Radix and Albizziae Cortex was evaluated to enhance the antitumor activity in vivo.