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자궁경부암 유전자 치료를 위한 재조합 p53 아데노 연관 바이러스 플라스미드 ( pAAVCMVp53 )의 제조
신봉영(Bong Young Shin),한유진(You Jin Han),조규남(Kyou Nam Cho),안웅식(Woong Shick Ahn),김진우(Jin Woo Kim),이준모(Jun Mo Lee),남궁성은(Sung Eun Namkoong),김수평(Soo Pyung Kim),이헌영(Hun Young Lee),김승조(Seung Jo Kim),김종국(Chong 대한산부인과학회 1998 Obstetrics & Gynecology Science Vol.41 No.11
N/A Objective: Human papillomavirus (HPV) has been identified in the majority of invasive cervical cancer patient and has been found to contribute in a significant way to the genesis of human cervical cancer. HPV has two transforming genes that encode the oncoproteins E6 and E7, E6 can form complexes with p53 and promote p53 degradation, E7 inhibit retinoblastoma protein (RB). The p53 protein is as a phosphoprotein which co-immunoprecipitated with the SV40 T-Antigen. The wild type p53 protein is capable of suppressing the tumorigenic phenotype and regulating cell cycle. Adeno-associated virus(AAV) is a linear single stranded DNA parvovirus which is dependent upon cotransfection by a second unrelated virus to undergo productive infection. It has been well documented that AAV DNA integrates into cellular DNA as one to several tandem copies joined to cellular DNA through the termini. In order to introduce wild type p53 through AAV virus into a cervical cancer patient for gene therapy, we had constructed recombinant p53 adeno associated virus plasmid (pAAVCMVp53). Methods: pAAVCMVp53 was created new AAV-vector system, pRc/CMVp53 including p53 cDNA and AAV-derivative vector, pASPA-AAV-CMV-polyA were made to HindIII/blunt fragments. Eluated 1.8 kb fragment of wild type p53 cDNA was ligated to pAAV-CMV-polyA, 4.9 kb fragment deprived hASPA cDNA. Result: Recombinant AAVCMVp53 was constructed by using pRc/CMVp53 andpASPA-AAV-CMV-polyA. This pAAVCMVp53 was confirmed by various restriction enzyme-digestions and Southern-blotting. This new vector system will be studied on expression, stability in cervical cancer cell lines and animals. Conclusion: This system will be one of the useful vector system for cervical cancer gene therapy.
정상 임신과 비정상 임신의 초기 혈중 CA - 125 수치에 관한 연구
노덕영(Duck Yeong Ro),김도강(Do Kang Kim),김수평(Soo Pyung Kim),문희봉(Hee Bong Moon),강규섭(Gyu Sub Kang),황지영(Jee Young Hwang),신봉영(Bong Young Shin),장병우(Byeung Woo Jang) 대한산부인과학회 1998 Obstetrics & Gynecology Science Vol.41 No.11
N/A A prospective study was initiated to compare maternal serum concentration of CA-125 during the first trimester of normal and abnormal pregnancies. Serum specimens were obtained from 87 women with a normal intrauterine pregnancy and 47 women with abnormal pregnancies which were ended in spontaneo abortion or pathologically confirmed to be missed abortion. In normal pregnancies, the mean serum CA-125 concentrations were increased significantly from amenorhea 6 weeks (139.838.7 IU/ml), and were higher statistically than the values tested in the same weeks of abnormal pregnancies. In abnormal pregnancies serum CA-125 concentations were relatively lower than those of normal pregnancies. But these differences were not statistically significant except the values tested in amenorhea 6weeks. So serum levels of CA-125 may not be proved useful in monitoring of early pregnancies outcome.