콩 첨가 증편의 Dextran과 내부구조에 관한 연구 ( Study on the Dextran and the Inside Structure of Jeung-Pyun of Adding Soybean )

신광숙,우경자 동아시아식생활학회 2001 동아시아식생활학회지 Vol.11 No.2

해바라기(Helianthus annus L.) 배양세포의 Cellular Peroxidase 활성을 억제하는 비병원성 Elicitor의 확인

송지연,신광숙,이인철 대전대학교 기초과학연구소 1999 自然科學 Vol.10 No.2

효모(Sacharomyces cerevisiae)의 세포벽 성분은 가장 잘 알려진 nonpathogenic elicitor이다. 이런 종류의 elicitor가 식물세포의 생리적 변화를 유도하는 기작을 규명하기 위하여 해바라기(Helianthus annus L.)의 배양 세포에 투여한 후 peroxidase 활성 변화를 측정하였다. 선행된 실험에서 보여주는 바 장미나 담배의 배양세포에서 yeast-elicitor는 peroxidase 의 활성, 특히 extracellular peroxidase 의 활성을 증가시켰으며, 기존의 peroxidase 와 구분되는 isoperoxidase 의 de novo synthesis를 유도한다는 증거를 얻은 바 있다. 그러나 해바라기 배양세포에서는 동일한 eliciting에 대하여 extracellular peroxidase의 활성변화는 관찰되지 않았으며 cellular peroxidase가 오히려 감소되는 결과를 얻었다. 효모 세포벽 성분을 DEAE-cellulose, CM-sepharose 및 concanavalin A-sepharose chromatography를 통하여 분획한 결과 DEAE-celluose로부터 용출된 분획과 concanavalin A로부터 용출된 분획이 peroxidase 의 활성을 억제하였으며 이러한 억제는 시료를 투석하여 glucan의 농도가 감소하는 것에 비례적으로 감소하였다. 그러므로 상기와 같은 반응을 유도하는 성분은 oligosaccharides라고 판단되었다. 이러한 결과는 동일한 elicitor에 대하여 식물의 종에 따라 반응하는 양식이 다름을 보여주는 결과라고 생각된다. Cell wall components of yeast (Sacharomyces cerevisiae) is well known nonpathogenic elicitor in some plant. To identify the chemical properties of yeast elicitor, the elicitor was isolated by ethanol precipitation followed by DEAE-cellulose, CM-sepharose and concanavalin A-sepharose chromatography. In contrast to the effect of elicitor in cultured cells of rose or tobacco, same elicitor from yeast has been found to suppress the peroxidase activity in cultured sunflower(Helianthus annus L.) cells. The inhibitory elicitor on peroxidase activity was detected in fractions eluted from DEAE-cellulose and from concanavalin A-sepharose column. The inhibitory effect of DEAE-fraction, however, was decreased by dialysis against distilled water using dialysis tubing with molecular cut off 1,000 Da. which suggested the yeast elicitor might be a oligosacharide. These results showed that plant might respond in different physiological ways to the same elicitor according to the plant species.

콩첨가에 따른 증편의 품질과 표면구조 변화

우경자,신광숙 한국조리과학회 1999 한국식품조리과학회지 Vol.15 No.3

Jeung-Pyun is a traditional fermented Korean food with rice flour, water, sugar, salt and unrefined rice wine(Takju). In order to investigate how the addition of soybean has an influence on Jeung-Pyun fermentation, changing to adding amount of soybean 0%, 5%, 10%, 15%, 20% based on rice weight, we carried out sensory evaluation and measured the physicochemical properties, instrumental characteristics and degree of gelatinization. The specific volume of Jeung-Pyun was increased as the more soybean was added. The pH of Jeung-Pyun batter was decreased as the fermentation time was longer, but it was increased as more soybean was added. The degree of gelatinization of Jeung-Pyun was decreased as storage day was longer, but it was high as more soybean was added compared to control(0%). In sensory evaluation, Jeung-Pyun added 5∼10% of soybean was good generally. Correlation coefficient between hardness and degree of gelatinization was high. In instrumental characteristics, hardness was increased only in control for room temperature storage(20℃), but at 4℃ it was less increased than control(0%) as more soybean was added. Cohesiveness was decreased generally as storage day was longer at 4℃, but it was less decreased as more soybean was added. In brittleness at 4℃, Jeung-Pyun of adding soybean was higher compared to control. In surface structure of Jeung-Pyun observerd by SEM, air pore size was small and distributed regularly as more soybean added. In conclusion, it can be suggested that the addition of soybean improve the quality of Jeung-Pyun.

콩첨가 증편의 미생물 변화와 품질 특성

우경자,신광숙,한영숙 동아시아식생활학회 1998 동아시아식생활학회지 Vol.8 No.2

The effect of soybean addition to the preparation of Jeung-Pyun(Korean fermented rice cake) and the fermentational characteristics of Jeung-Pyun dough were determined. Jeung-Pyun dough composed of rice 100g, soybean 20g, water 40g, salt 0.8g, sugar 15g and Tak-ju 30g was fermented for up to 20 hours. The numbers of total microbes, yeasts and lactic acid bacteria in the dough during fermentation increased rapidly during 5 hours of fermentation, but Leuconostoc mesenteroides during 7 hours of fermentation. Generally, there were many more microbes in the Jeung-Pyun dough with soybeans than in the Jeung-Pyun dough with rice. The volume of 7 hour fermented Jeung-Pyun was most massive especially in the soybean-added Jeung-Pyun. In 1% dextran-added rice Jeung-Pyun, the volume of steamed cake was increased more than that of rice Jeung-Pyun, but it was not as bulky as in the soybean-added cake. In the sensory evaluation of the test cake products, the 7 hour fermented cake was preferred by most. As for instrumental characteristics, the 7 hour fermented Jeung-Pyun showed the lowest values for hardness and elasticity, brittleness and chewiness. The rice Jeung-Pyun showed higher values for hardness and brittleness than in soybean-added Jeung-Pyun. When the Jeung-Pyuns were stored at 20℃, the hardness, brittleness and cohesiveness of rice Jeung-Pyun were respectively increased but not in the soybean-added Jeung-Pyun. However, the hardness of rice Jeung-Pyun kept at 4℃ increased rapidly after 5 days of storage. The cohesiveness, elasticity, brittleness, chewiness, gumminess of both rice Jeung-Pyun and soybean-added Jeung-Pyun were decreased with storage time.

슬관절 전치환술에서 최근 논란 사안에 대한 환자 선호도

최진배(Jin Bae Choi),박관규(Kwan Kyu Park),신광숙(Kwang Sook Shin),장종범(Chong Bum Chang),정헌(Heon Jung),성상철(Sang Cheol Seong),김태균(Tae Kyun Kim) 대한정형외과학회 2007 대한정형외과학회지 Vol.42 No.2

목적: 슬관절 전치환술에 있어서 최근 논란 사안들에 대한 환자의 사전 지식과 선호도를 조사하고, 의료진에 의한 설명이 환자의 선택에 미치는 영향을 조사하고자 하였다. 대상 및 방법: 본원에 슬관절 전치환술을 시행하기 위하여 내원한 총 100명의 환자들을 대상으로 하여 양측동시 수술, 컴퓨터 항법 인공관절술과 최소 침습 인공관절술 그리고 세라믹 인공관절술에 대한 사전지식의 정도와 선호도를 평가한 뒤 다시 본원에서 작성한 설명문을 통하여 연구자가 환자들에게 그들의 장단점에 대하여 설명한 뒤 환자로 하여금 수술 방법을 다시 선택하게 하여 설명 전과 설명 후의 차이를 분석하여 보았다. 결과: 환자들은 대부분 충분한 사전 지식을 지니지 못하였고 또한 비전문가를 통한 정보에 의존하고 있었다. 환자들은 대부분 설명 전에는 새로운 기법을 선호하였지만 설명을 듣고 난 후에는 대부분 안전하고 정확하며 입증된 방법을 선택하였다. 또한 남성 환자들은 양측 동시 수술을 선호하는 것으로 나타났고 젊은 환자들의 경우 세라믹 소재를 선호하는 것으로 나타났다 결론: 환자들은 슬관절 전치환술의 최신 치료방법에 대하여 아주 제한된 지식을 가지고 있으며 그들의 선호도는 의료진에 의하여 대부분 좌우된다. 그러므로 환자들의 올바른 선택을 위해서는 균형 잡힌 수술 전 설명이 필요할 것이다. Purpose: To examined the level of patient knowledge and their perspectives about the controversial issues in total knee arthroplasty (TKA), and to evaluate the effect of a relevant explanation about the issues on the patient preferences for their particular option. Materials and Methods: One hundred patients who visited our clinic and decided to undergo TKA were asked to complete a questionnaire asking about their knowledge and preference for 4 controversial issues: 1) surgical timing of the bilateral TKAs, 2) use of computer assisted surgery, 3) use of minimal invasive surgery, and 4) use of ceramic femoral component. The patients completed the same questionnaire after the advantages and disadvantages of each option had been explained using an explanatory document designed based upon what was documented in the literature. Results: The patients were not well-informed about the issues and received their information through a non-professional source. The patients tended to prefer new options with claimed promises before an explanation. The patients preferred the options with safety, accuracy, and proven evidence after an explanation. Male patients tended to prefer simultaneous TKAs more than female patients. Younger patients preferred ceramic femoral component claimed to have better longevity more than older patients did. Conclusion: This study demonstrates that patients' knowledge of the current controversial issues is very limited, and that patient preferences would be significantly changed if they were given an explanation from a physician. Balanced information should be given to patients in order for them to reach a fair decision.

사이토카인 유전자 함유 바이러스 유사입자의 제조

오유경(Yu Kyoung Oh),손태종(Tae Jong Son),신광숙(Kwang Sook Sin),강민정(Min Jeong Kang),김정목(Jung Mogg Kim),김남근(Nam Keun Kim),고정재(Jung Jae Ko),김종국(Chong Kook Kim) 한국약제학회 2001 Journal of Pharmaceutical Investigation Vol.31 No.3

N/A Human papillomavirus (HPV) infection is known to cause cervical cancers. Human papillomavirus-like particles (VLP) have been studied as preventive vaccines of cervical cancers. To develop VLP as a therapeutic gene carrier, we studied the method to encapsulate cytokine genes in virus-like particles. HPV type 16 capsid L1 genes were amplified by polymerase chain reaction and cloned into T vector. L1 gene was then inserted into baculovirus transfer vector. The clone of baculovirus encoding L1 gene was isolated and used to express L1 protein in Sf 21 insect cells. VLP were purified by CsCl density gradient and ultracentrifugation. VLP were disassembled to capsomer units by treatment of a reducing agent. Given that interleukin-2 (IL-2) genes have been used in anticancer gene therapy and as a molecular adjuvant, IL-2 cytokine plasmids were chosen as a model gene. IL-2 plasmids were incubated with the disassembled capsomer suspension. To reassemble the particles, the mixture of capsomers and cytokine plasmids was dialyzed. The disassembly and reassembly of VLP were confirmed by transmission electron microscopy. The entrapment of cytokine plasmids in reassembled VLP was tested by the stability of plasmids against DNase I. After treatment of reassembled virus-like particles with DNase I, discrete IL2 DNA band was observed. Our results indicate that IL-2 cytokine plasmid (3.5 kb size) can be encapsulated in the viruslike particles, suggesting the potential of VLP as a gene delivery system. Moreover, VLP containing the adjuvant cytokine plasmids might function as more effective subunit vaccines.

연구논문 : 생명과학 ; Polyethylenimine를 매개체로 한 cytokine plasmid DNA의 세포포집, 핵 전이 및 익스프레션

오유경 ( Yu Kyoung Oh ),서동철 ( Dong Chul Suh ),김정목 ( Jung Mogg Kim ),최한곤 ( Han Gon Choi ),신광숙 ( Kwang Sook Shin ),고정재 ( Jung Jae Ko ) 영남대학교 약품개발연구소 2003 영남대학교 약품개발연구소 연구업적집 Vol.13 No.-