섬유소 분해효소 생성을 위한 Trichoderma viride 조절 변이주의 개발

송은경,허태린,이세영 ( Eun Kyong Song,Tae Lin Huh,Se Yong Lee ) 생화학분자생물학회 1982 BMB Reports Vol.15 No.3

An attempt were made to isolate regulatory mutants of Trichoderma viride using a plating technique devised to screen constitutive cellulase mutants and high cellulase producing mutants. With U.V. mutagenesis, 70 mutants which exhibited high cellulase activity in the presence of 2% glucose were initially isolated from Trichoderma viride QM9414, a high celluase producing U.S. Army Natick Laboratory Strain. Upon continuous culture in the liquid medium, however, most of these mutants. were reverted to the glucose sensitive strain. One of the mutant, L40, was stable even after long period of culture in the liquid medium and still showed a high cellulase activity and relative insensitivity to glucose for cellulases production. The characteristics of the mutant L40 and cellulases produced by this mutant were as. follows: 1. When cellulases were induced by Avicel, a crystalline cellulose, the mutant L40 produced approximately 47 % higher cotton activity than the parental strain QM 9414. The induction time for the cotton activity was also shortened appreciably in. the mutant. CMC activity and β-glucosidase activity, however, were not changed in their total activity or induction time. 2. The cellulases induction by Avicel were less sensitive to glucose in mutant L40 than in the parental strain QM9414. 3. When cellulases were induced by carboxy methyl cellulose, a noncrystalline cellulose, there were no appreciable changes in cellulases activities, nor in induction times. 4. When cellulases were induced by cellobiose, the mutant L40 produced approximately 40 % higher cotton activity than the parental QM9414 and the induction time was shortened appreciably in the mutant. The CMC activity was also changed in the mutant but no change in β-glucosidase activity was observed. 5. The enzymic properties of cellulases and the growth characteristics of mutant L40 and the parental QM9414 were the same.

섬유소 분해효소 생성을 위한 Trichoderma viride 조절 변이주의 개발

송은경,허태린,이세영,Song, Eun-Kyong,Huh, Tae-Lin,Lee, Se-Yong 생화학분자생물학회 1982 한국생화학회지 Vol.15 No.3

구성변이주와 높은 섬유소 분해력을 갖는 돌연변이주를 검색하는 데 이용되는 평판배양법을 써서 Trichoderma νiride의 조절 변이주를 선별하고자 하였다. U.V.를 돌연변이 유발원으로 사용하여, U.S. Army Natick Laboratory균주로써 높은 섬유소 분해력을 갖고 있는 Trichoderma νiride QM9414로부터 2%의 glucose존재하에서도 높은 섬유소 분해력을 나타내는 돌연변이 균주 70개를 선별해서 액침배양한 결과 이들 중 대부분이 glucose에 의해 repression을 받는 것으로 나타났으나 돌연변이 균주의 하나인 L40은 오랜동안의 액침 배양 후에도 높은 섬유소분해력을 보여주었으며, 섬유소 분해효소 생성에 있어서 glucose에 의해 비교적 repession을 덜 받는 것으로 나타났다. 변이주 L40과 변이주 L40에 의해 생성된 섬유소 분해효소의 특성은 다음과 같다. 1. 결정형 섬유소인 Avicel을 유도기질로 사용하여 섬유소 분해효소를 유도하였을 때 결정형 섬유소 분해력인 cotton activity가 약 47 % 증가하였으며 효소 유도시간도 모균주인 QM9414보다 상당히 단축되었다. 비결정형 섬유소 분해력인 CMC activity와 cellobiose를 glucose로 분해하는 ${\beta}$-glucosidase activity에는 큰 변화가 없었다. 2. Avicel을 유도기질로 사용하여 섬유소 분해효소를 유도하였을 경우 돌연변이 균주인 L40이 모균인 QM9414보다 glucose에 의해 repression을 덜 받았다. 3. 비결정 섬유소인 carboxymethyl cellulose로 섬유소 분해효소를 유도했을 때는 섬유소 분해효소의 활성이나 시간이 모균주인 QM9414와 거의 비슷했다. 4. Cellobiose로 섬유소 분해효소를 유도했을 때 돌연변이주 L40의 결정형 섬유소분해력이 모균주 QM9414보다 약 40%증가하였고 효소 유도시간도 약간 단축되었으며 비결정형 섬유소 분해력인 CMC activity도 약간 증가하였으나 유도시간에는 변화가 없었고 ${\beta}$-glucosidase activity는 그 역가에 있어서나 유도시간에 있어서 별다른 변화가 없었다. 5. 변이주인 L40이 모균인 QM9414에 있어서 섬유소 분해효소의 효소적 특성과 성장상의 특정의 차이점은 없었다. An attempt were made to isolate regulatory mutants of Trichoderma viride using a plating technique devised to screen constitutive cellulase mutants and high cellulase producing mutants. With U.V. mutagenesis, 70 mutants which exhibited high cellulase activity in the presence of 2% glucose were initially isolated from Trichoderma viride QM9414, a high celluase producing U.S. Army Natick Laboratory Strain. Upon continuous culture in the liquid medium, however, most of these mutants. were reverted to the glucose sensitive strain. One of the mutant, L40, was stable even after long period of culture in the liquid medium and still showed a high cellulase activity and relative insensitivity to glucose for cellulases production. The characteristics of the mutant L40 and cellulases produced by this mutant were as. follows: 1. When cellulases were induced by Avicel, a crystalline cellulose, the mutant L40 produced approximately 47 % higher cotton activity than the parental strain QM 9414. The induction time for the cotton activity was also shortened appreciably in. the mutant. CMC activity and ${\beta}$-glucosidase activity, however, were not changed in their total activity or induction time. 2. The cellulases induction by Avicel were less sensitive to glucose in mutant L40 than in the parental strain QM9414. 3. When cellulases were induced by carboxy methyl cellulose, a noncrystalline cellulose, there were no appreciable changes in cellulases activities, nor in induction times. 4. When cellulases were induced by cellobiose, the mutant L40 produced approximately 40 % higher cotton activity than the parental QM9414 and the induction time was shortened appreciably in the mutant. The CMC activity was also changed in the mutant but no change in ${\beta}$-glucosidase activity was observed. 5. The enzymic properties of cellulases and the growth characteristics of mutant L40 and the parental QM9414 were the same.

부모와의 경제적 교류여부 및 교류액의 결정요인

김지경(Kim Ji-Kyung),송은경(Song Eun-Kyong) 가정과삶의질학회 2004 가정과삶의질연구 Vol.22 No.5

임베디드 소프트웨어 인력양성

유준혁(Joon-Hyuk Yoo),이신재(Sin-Jae Lee),송은경(Eun-Kyong Song),최진영(Jin-Young Choi),유혁(Chuck Yoo) 한국정보과학회 2009 정보과학회지 Vol.27 No.1

중환자실 간호사들의 눈 간호에 대한 지식, 인식 및 수행 실태

오의금(Oh Eui Geum),추상희(Chu Sang Hee),고일선(Ko Il Sun),이원희(Lee Won Hee),유지수(Yoo Ji Soo),김소선(Kim So Sun),유해영(Yoo Hae Young),송은경(Song Eun Kyeung),강세원(Kang Se Won),김복희(Kim Bok Hee),조경미(Cho Kyong Mi),최정민(Choi 기본간호학회 2007 기본간호학회지 Vol.14 No.4

Purpose: The purpose of this study was to identify levels of knowledge, perception, and practice of eye care as part of nursing care in the ICU. Method: A descriptive, cross-sectional study design was used. The participants in this study were 269 registered nurses working in the ICUs of five university hospitals in Seoul, Korea. Between November 2005 and December 2005, data were collected using a semi-structured questionnaire developed by the researchers, and analyzed using the SPSS Program. Results: In general, levels of knowledge of nursing care for the eyes were moderate among ICU nurses, but levels of perception of nursing care for the eyes were very high. Of the respondents, 61.7% reported that they would consult a doctor whenever they found an eye problem. Only 42.4% nurses answered that they provide eye care as part of the daily routine and 43.9% nurses responded that they cleanse the eye lids with wet saline gauze. In regression analysis, the practice of eye care as part of nursing was significantly influenced by perception of nursing care for the eyes, and knowledge of interventions for nursing care for the eyes. Conclusion: These results suggest that education on care of the eyes for ICU nurses and the development of a standardized eye care protocol should be done to improve quality of nursing care in the ICU.

상염색체 우성 다낭신에서의 신낭종 경화요법

김연수(Yon Su Kim),안규리(Cu Rie Ahn),한진석(Jin Suk Han),김성권(Shung Gwon Kim),이정상(Jung Sang Lee),이중건(Jung Geon Lee),윤성철(Sung Chul Yoon),박종훈(Jong Hoon Park),송은경(Eun Kyong Song),황대연(Dae Yeon Hwang),박정환(Jung Hwan 대한신장학회 2002 Kidney Research and Clinical Practice Vol.21 No.4

배 경 : 상염색체 우성 다낭신(ADPKD)은 성인에서 가장 흔한 유전성 신질환의 하나로서 낭종의 팽창으로 인한 복부 팽만이나 통증을 흔히 호소한다. 이의 치료를 위해 에탄올을 이용한 경화요법이나 복강경을 이용한 치료, 수술적 감압술 등이 사용되어지고 있다. 본 연구에서는 한국인 ADPKD 환자에서의 경화요법 후의 임상경과를 분석하고, 새로운 경화제인 n-butyl cyanoacrylate(NBCA)의 유용성을 검토하였다. 방 법: 저자들은 한국인 ADPKD 환자 24명을 대상으로 에탄올경화요법(n=9) 및 NBCALipiodol 혼합액을 이용한 경화요법(n=18)(3예는 두가지 치료법 병행 )을 시술하고 이들의 임상 경과를 추적 관찰하였다. 결 과: 대상 환자의 남녀비는 8 : 16이었고 치료 시 평균연령은 50±13세였다. 경화요법의 적응증으로는 동통이 14예, 복부 팽만이 7예였으며, 흡인된 낭종의 직경은 5 내지 16 ㎝으로 다양하였다. 동통이나 복부팽만은 2명을 제외한 전원에서 주관적인 개선을 보였다. 시술 전후의 평균동맥압은 시술 전 112±11.1, 시술 후 1개월 96±9.6, 시술 후 6개월에 98±9.7 ㎜Hg로 유의한 저하를 보였으나(p<0.05), 이러한 효과는 12개월째에 소실되었다. 혈중요소질소 농도는 기저치와 시술 후 6개월 측정치 사이에 유의한 차이가 없었다[24±12.1 대 22±14.6(㎎/dL)]. 출혈이나 감염과 같은 주요 합병증은 발생하지 않았으며, 시술과 관련된 사망례도 없었다. 에탄올과 NBCA 사용군 사이에 치료효과나 합병증에서 차이는 관찰되지 않았다. 결 론: ADPKD 환자에서의 신낭종 경화요법은 대부분에서 증상의 완화를 보였고, 일시적인 혈압강하 효과를 보였으며, NBCA는 신낭종 치료에 새롭게 사용된 경화제로서 단기관찰 결과 유용하고 안전한 것으로 생각된다. Background : Autosomal dominant polycystic kidney disease(ADPKD) is the most common hereditary renal disease in adults, and its major complaints include pain and abdominal fullness due to cyst expansion. So far, for the control of these symptoms, cyst ablation with ethanol or tetracycline, laparoscopic manipulations and surgical marsupialization have been used. Methods : We used conventional ethanol(n=9) or n-butyl cyanoacrylate(NBCA) plus lipiodol solution (n=18) or both(n=3) for separate cysts as the sclerosing agent in 24 adult Korean ADPKD patients. And their clinical courses after treatment were evaluated. Results : The male to female ratio was 8 : 16 and the mean age at the treatment was 50 yrs(S.D. 13.1). Causes for aspiration were pain in 14 and abdominal fullness in 7 patients and the range for the cyst diameters aspirated were 5-16 cm. Flank pain or discomfort were decreased subjectively in most cases except two. Mean arterial pressures(S.D.) (㎜Hg) before and after procedure were as follows 112(11.1)(basal), 96(9.6)(1 month) and 98(9.7)(6 month)(p<0.05, paired-t test). Blood urea nitrogen levels(㎎/dL) were not changed 6 month later[24 (12.1) vs. 22(14.6)]. There was no major complication such as bleeding or infection and no death and associated with procedure. There was no difference of therapeutic effect according to sclerosing agent. Conclusion : NBCA was as effective as conventional ethanol for sclerotherapy in ADPKD and cyst ablation therapy showed a BP-lowering effect in short-term period.