분자생물학적 방법인 PCR-REBA를 이용한 대중목욕탕 수질 중 수인성병원성미생물 검출

송운흥(Song, Woon-Heung),최승구(Choi, Seung-Gu),양병선(Yang, Byoung-Seon),이재상(Lee, Jae-Sang) 한국산학기술학회 2011 한국산학기술학회논문지 Vol.12 No.8

수인성 병원성 미생물에 의한 공중목욕탕의 오염은 질병발생의 원인이 된다. 본 연구에서는 공중목욕탕내에 존재하는 수인성 병원성미생물들을 확인하고자 하였다. 서울지역내의 30 곳 공중목욕탕에서 욕조수 시료를 채수하여 진행하였다. 수인성 병원성미생물의 검출은 0.45 ㎛의 여과막을 이용하여 전통적인 배양방법으로 분리 및 동정하였 다. 분자생물학적 기법을 사용하기 위해 미생물학적인 배양을 하지 않고 핵산을 추출하여 16S rRNA유전자를 표적으 로 polymerase chain reaction-reverse blot hybridization (PCR-REBA)을 실시하였다. 미생물학적 배양방법에서는 지표 세균인 Escherichia coli와 Shigella spp.가 검출되었으며, 분자생물학적 기법인 PCR-REBA을 수행한 결과 E. coli, Shigella spp., Salmonella spp., Pseudomonas spp., Mycobacterium spp. 등의 수인성 병원성미생물이 7곳에서 검출되었 다. 본 연구결과를 토대로 공중목욕탕의 욕조수내에 수인성병원성미생물에 의한 감염을 줄이기 위해 적절한 위생관 리과 E. coli를 포함한 유해미생물을 선정하여 지속적인 모니터링이 필요할 것으로 사료된다. Contamination of public bath water by waterborne pathogens can cause disease outbreaks and contribute to background rates of disease. The aim of this study is to determine the prevalence of waterborne pathogens in public baths. A total of 30 water samples were collected from 30 different public baths in seoul, Korea. Pathogens in water samples were concentrated by 0.45 ㎛ nitrocellulose membrane filter, analyzed by both cultivation and polymerase chain reaction-reverse blot hybridization (PCR-REBA) of partial 16S rRNA gene. Various microorganisms including Escherichia coli and Shigella spp. were identified by microbiological cultivation. E. coli, Shigella spp., Salmonella spp., Pseudomonas spp. and Mycobacterium spp. were identified by PCR-REBA. Our results suggest that appropriate hygiene practice and continuous monitoring is needed for reducing health risk associated with public bath houses.

Coulter 자동혈구계 산기 (Coulter S Plus IV) 의 백혈구 histogram 에서 이상을 보이는 검사결과의 검토

송운흥 ( Woon Heung Song ),박효순 ( Hyo Soon Park ),조한익 ( Han Ik Cho ) 대한임상검사과학회 1990 대한임상검사과학회지(KJCLS) Vol.22 No.1

1957 년 aperture-impedance 혈구계 산법 이 개 발 된 이래 자동혈구계산기는 검사실 내의 일반혈액검사의 업무를 혁신적으로 변화시켰다 1) 즉 단시간에 다량의 검체를 능률적으로 재현성 있는 검사를 자동혈구계산기를 이용해서 할 수 있게 되었다. 저자들은 일반혈액검사업무의 자체 정도관리 중에 서울대학교 병원에서 사용하는 혈구계산기인 Coulter 5 Plus IV (Coulter Electronics, INC, Hialeah: 이 하 Coulter) 기종의 검사값 중에서 백혈구 histogram에 이상을 보이븐 값들이 수기법으로 재검한 값과 차이가 있음을 발견하였다. 이들은 기계에서 여러 형태로 표지를 해주게 되는데 이 표지된 백혈구 histogram 에 이 상이 있는 검 사값이 International Committee for 5tandardization in Hematology (IC5H) 가 추천하는 기준검사법인 수기법과 차이가 있는지, 또 다른 원리 인 light scattering을 이 용하는 혈구계 산기 인 H -1 5ystem (T echnicon Instrument Corporation. Tarrytown: 이 하 H-1) 과는어떤 차이가 있는지를 알고 정확하고 신뢰성 있는 일반혈액검사를 위해 이 연구를 시행하였다 2-4) CoulterS Plus series hematologic autoanalyzers (Coulter Electronics, INC. Hialeah) have long been the mainstay of the routine hematology laboratories in Korea. The analyzer flaggs results with abnormal histograms or data out of the range specified, but those flaggs are often neglected and data with them are reported to physicians. We found that the results with abnormal histogram (high take off) are reallu spurious and proceeded the study comparing thoose with results from the manual chamber counting (ICSH reference metho.

영상의학과 흉부 엑스선 촬영 기기의 세균 오염도

최승구(Seung Gu Choi),송운흥(Woon Heung Song),권대철(Dae Cheol Kweon) 대한방사선과학회 2015 방사선기술과학 Vol.38 No.4

영상의학과의 흉부엑스선 검사에서 감염관리에 대한 기기의 오염 정도를 세균학적으로 확인하고 균을 동정하여 병원감염의 예방과 감염관리방안을 마련하는데 목적이 있다. 영상의학과 흉부 엑스선 검사에서 환자의 기기 접촉부위인 어깨, 손, 턱, 흉부 측면촬영의 손잡이 부위를 70% 이소프로필 알코올로 소독하기 전과 후를 면봉으로 검체를 채취하여 세균을 동정하여 비교하여 분석하였다. 흉부 엑스선 기기에서 소독전의 측면촬영의 손잡이에서 그람 양성의 Staphylococcus가 분리되었다. 최종동정을 위해 노보바이오신 (nobobiocin) 항생제 시험을 실시하여 민감성으로 Staphylococcus epidermidis로 확정하였다. 흉부촬영 기기에서 측면촬영의 손잡이 부위를 소독 전에 Staphylococcus epidermidis 균이 검출되었다. 흉부엑스선 검사에서 기기를 주기적인 소독 및 멸균을 실시하여 병원감염을 예방하는 관리방안을 마련하여야 한다. The purpose is to determine the degree of contamination of the equipment for infection control in chest radiography of the radiology department. We confirmed by chemical and bacterial identification of bacteria of the equipment and established a preventive maintenance plan. Chest X-ray radiography contact area on the instrument patients shoulder, hand, chin, chest lateral radiography patient contact areas with a 70% isopropyl alcohol cotton swab were compared to identify the bacteria before and after sterilization on the patient contact area in the chest radiography equipment of the department. The gram positive Staphylococcus was isolated from side shoots handle before disinfection in the chest radiography equipment. For the final identification of antibiotic tested that it was determined by performing the nobo-biocin to the sensitive Staphylococcus epidermidis. Chest radiography equipment before disinfecting the handle side of Staphylococcus epidermidis bacteria were detected using a disinfectant should be to prevent hospital infections.

원저(原著) : 공혈자 선별을 위한 HCV-SPOT 검사의 평가

류광현 ( Kwang Hyun Ryu ),송운흥 ( Woon Heung Song ) 대한임상병리사협회 1996 임상수혈검사학회 발표자료집 Vol.3 No.1

Hepatitis C is a major problem of post-transfusion hepatitis. The best measure for preventing hepatitis C infection after transfusion is blood donor screening. There are many methods for detecting hepatitis C virus antibodies. EIA is one of the widely used, sensitive method. However it needs time consuming, but HCV-SPOT is a simple, rapid test for the detection of anti-HCV. We evaluated comparative results in detection of HCV antibodies by using a rapid HCV test and EIA. The study shows following results. The false positive was 8.3 %(3.36), false negative was 0 %(0.58), sensitivity was 100 %(54.54), specificity was 92.5 %(37.40), accuracy was 96.8 %(91.94). This study relatively shows high efficiency of SPOT test. But this rapid test must use emergency state.

원저(原著) : Aliquot bag을 이용한 소아용 적혈구 제제의 사용 경험

류광현 ( Kwang Hyun Ryu ),송운흥 ( Woon Heung Song ) 대한임상병리사협회 1997 임상수혈검사학회 발표자료집 Vol.4 No.1

It is important to supply the small volume of blood for pediatric and neonatal transfusion. Anemia in newborns may result from red blood cell loss due to the multiple blood sampling for laboratory test or from pathological bleeding. Because small volume requirements of transfusion to newborns may give rise to a large amount of wastage and multiple donor blood exposure that blood bank providing blood for neonates have developed preparing 50~140 ml pediatric units of red blood cells. The authors experienced the use of aliquot bags for pediatric units of red blood cells in the Blood Bank, Samsung Medical Center since October 1994.

비만도에 따른 성인들의 집단별 골밀도 분석

김순근,권대철,오찬호,송운흥,Kim, Sun-Geun,Kweon, Dae-Cheol,Oh, Chan-Ho,Song, Woon-Heung 대한디지털의료영상학회 2008 대한디지털의료영상학회논문지 Vol.10 No.1

Obesity which causing many physical and psychological problems is difficult to treatment. The purpose study was investigate relationships between weight, height, body mass index (BMI) and BMD of according to the low, normal, over, obesity, high group. According to obesity index, each group was divided into five proup. Study subjects were man(n=135) and woman(n=145). We checked the BMD of femoral neck and lumbar spine by using dual energy X-ray bone densitometry. Weight was positively correlation with height (p<0.05) by Pearson's correlation matrix. There was a significant difference in BMD of femoral neck and lumbar spine between four(low, normal, over, obesity) groups except for high obesity groul.

PCR-RFLP에 의한 대중목욕탕 내 Nontuberculous Mycobacteria의 동정

최승구 ( Seung Gu Choi ),송운흥 ( Woon Heung Song ),강치환 ( Chee Hwan Kang ),조규봉 ( Kyu Bong Cho ),이재상 ( Jae Sang Lee ),이장호 ( Jang Ho Lee ),김성일 ( Sung Il Kim ),지수일 ( Soo Il Jee ) 대한임상검사과학회 2008 대한임상검사과학회지(KJCLS) Vol.40 No.1

Thirty two of bathroom water samples from public bathroom in Seoul areas were examined using acid-fast staining, Lowenstein-Jensen (L-J) medium culture and PCR-restriction fragment length polymorphism (PCR-RFLP). In 6.25% (2/32) bathroom water samples, acid-fast bacilli were detected by AFB stain, and in 21.9% (7/32) bathroom water samples, acid fast bacilli grew on L-J media. Of them, six acid-fast bacilli were identified as Mycobacterium avium, and the other AFB as Mycobacterium szulgai by PCR-RFLP. These results are suggested that accidental nontuberculosis mycobacterial infection to a weakness person will be possible in public area.

혈중 OKT3 농도 측정방법 소개

김정해 ( Jeong Hae Kim ),송운흥 ( Woon Heung Song ) 대한임상병리사협회 1999 임상혈액검사학회 발표자료집 Vol.5 No.1

장기이식이 활성화되면서 그에 따른 면역거부 반응의 예방 및 치료효과를 검사할 수 있는 방법도 다양해지고 있다. 그 중 가장 강력한 면역억제제로 알려진 것이 OKT3 Monoclonal antibody이다. 이는 T-lymphocyte에 작용하여 그의 기능을 차단하거나 T-lymphocyte를 제거함으로서 면역거부반응을 예방 및 치료할 수 있는 것이다. 실지로 OKT3를 투여하면 1시간 후 혈중농도가 약 980ng/ml까지 증가하며 T-lymphocyte는 급격히 감소된다. 24시간 후 OKT3농도는 약 195ng/ml로 감소되면서 3일간 약 950 ng/ml로 증가하여 이 농도가 지속된다. 마지막 투여 후에는 약 1006ng/ml까지 증가하였다가 3일 후에는 약 20ng/ml로 감소한다. 그러므로 혈중 OKT3의 농도를 monitering하는 것이 무엇보다도 중요하다. 하지만 OKT3의 농도가 충분하고 T-lymphocyte가 감소가 되더라도 거부반응을 보인 예가 약 10% 있다고 보고되었다. 이런 경우는 T-lymphocyte가 "dimly positive" 를 보인 경우였 다. 따라서 OKT3가 거부반응의 절대적 치료방법은 아니지만 가장 많이 사용되는 면역억제제이다. 일반적으로 치료효과를 볼 수 있는 OKT3의 혈중 농도는 800ng/ml 이상이며 T-lymphocyte의 수는 10~50/ μl 이하여야 한다고 알려져 있다. 본원에서의 OKT3검사방법은 농도를 알고 있는 OKT3와 환자혈청을 정상인의 Lymphocyte와 반응시킨 후 형광염색을 하여 flowcytometry로 분석하여 표준농도 곡선을 그려 환자의 혈중 농도를 구하고 있다. 대부분의 병원에서는 T-lymphocyte 만을 검사하여 간접적으로 OKT3의 치료경과를 관찰하나 혈중 OKT3의 농도를 함께 검사함으로서 보다 신뢰성 있는 결과를 제공할 수 있다고 생각된다. During the last few years, monoclonal antibodies have represented a new specific therapeutic approach in the prevention of allograft rejection. OKT3 is very potent immunosuppressive agent that used in the treatment of rejection as well as in some induction therapy protocols. It is considered that the therapeutic effectiveness of OKT3 is related to the effect of this monoclonal antibodies in blocking the function and T cell depletion. The plasma OKT3 level at 1 hour after the first injection was about 980 ng/ml and decreased to about 195 ng/ml at 24hours.Subsequently, the 24-hour trough level of OKT3 increased to about 950 ng/ml by day 3 and remained steady during the period of the treatment. After the last dose. the mean trough level of plasma OKT3 was 1006 ng/ml at 24 hours and then decreased during the three subsequent 24-hour periods to about 480 ng/ml, 220 mg/ml, 20 ng/ml, respectively. Therefore, the evaluation of plasma levels is important in determining its effectiveness in blocking cellular rejection. But 10% patients who were treated with OKT3 experienced recurrence of rejection despite the absence of CD3+ cells in the peripheral blood and the excess of OKT3 in the plasma. In this case, "Dimly positive cm" lymphocytes was detected. Therefore, OKT3 therapy was not absolute. But it is the most potent immunosuppressive agents clinically available. Generally, studies have suggested that OKT3 serum concentrations >800 ng/ml and CD3+ cells 10~50/ μl are associated with in vitro blocking of T cell effector function. In this study, techniques to monitor OKT3 serum levels utilize flowcytometry. Fresh lymphocytes were added to plasma samples and incubated on ice. Fluorescence intensity was determined by means of FACSort. The standard curve was obtained by serial dilutions of OKT3 corresponding to well-known quantities of the monoclonal antibodies. The flowcytometric monitoring seems to be a useful procedure to evaluate plasma OKT3 levels in transplanted patients with OKT3 prophylaxis.

인간 거대세포 바이러스 감염 진단을 위한 CMV 항원혈증검사의 유용성 평가

이장호 ( Jang Ho Lee ),배재춘 ( Jae Chun Bae ),송운흥 ( Woon Heung Song ),이남용 ( Nam Yong Lee ) 대한임상검사과학회 2000 대한임상검사과학회지(KJCLS) Vol.32 No.3

Early detection and treatment of human cytomegalovirus(CMV) infection is very important because CMV infection is major cause of morbidity and mortality after organ transplantation. CMV antigenemia assay has been reported to be very sensitive and specific for detection of CMV infection among many laboratoη methods. We compared the results of CMV antigenemia(Clonab CMV kit; Biotest AG, Landsteinerstrasse, Dreiceich, Germany), CMV PCR, CMV culture and CMV IgM to evaluate their clinical usefulness. We compared 620 s없nples for CMV antigenemia tests, 2,272 samples for CMV culture, and 423 samples for CMV PCR tests between July 1997 and April 1998 in samsung medical center clinical microbiology For CMV test, the sensitivity of CMV culture, CMV antigenemia, and CMV PCR were 4.3%, 16.6% and 31.0%, respectively. Sixty seven patients were classified CMV antigenemia positive. The results of compared for CMV culture, CMV PCR, and CMV IgM antibody. For CMV antigenemia, the sensitivity of CMV PCR and CMV IgM antibody were 85.3%, 93.5% respectively. CMV antigenemia is thought to be useful for early diagnosis and follow- up of anti띠ral treatment as a quantitative and highly specific method. The sensitivity and specificity are very comparing to CMV antigenemia.

원저(原著) : 건강의학센터 내원자들이 알고 있는 혈액형의 정확성에 대한 조사

유혜영 ( Hye Young Yu ),류관현 ( Kwang Hyun Ryu ),송운흥 ( Woon Heung Song ) 대한임상병리사협회 1997 임상수혈검사학회 발표자료집 Vol.4 No.1

In order to investigate the discrepancy rate between actual and believed groups. ABO blood grouping were performed in the visitors of Health Promoting Center, Samsung Medical Center. The results were as follows : 1. Among 5,245 visitors discrepancies were found in 335, and the discrypancy rate was 6.4% 2. The discrepancies were found 123(36.7%) in group AB, 77(23.0%) in group B, 73(21.8%) in group A, and 62(18.5%) in group O.