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손정필,성승현,서동상 성균관대학교 생명과학자원연구소 1995 生命資源科學硏究 Vol.2 No.1
Transfection, gene transfer into mammalian cells, is used to study gene structure and function. In this study, transfection of Sp2/0 cells using LK4LAC plasmid DNA(containing β-galactosidase gene) with various parameters was investigated. The transformants were identified by the β-galactosidase staning method. Optimal transfection conditions of Sp2/0-Ag14 cells were studied with two methods, calcium phosphate(CaPO_4) precipitation method and liposome method. In calcium phosphate precipitation method, the highest transfection efficiency was obtained when 20㎍ of DNA was transfected for 12hours and incubated for 16hours. In case of liposome method, the optimal. condition was achieved when 25㎍ of lipofectin amount was transfected for 18hrs. The optimal drug concentration of selection with G418 was 400㎍/㎖ in the 20 cells/dish of Sp2/0-Ag14 cell line.
손정필 順天鄕大學校 學生生活硏究所 1996 學生生活硏究 Vol.3 No.-
The purpose of this study was to develop a career search training program for college students. This training program was designed as follows : 1. Understanding career concept as human development aspect. 2. Certifying realistic conditions for oneself which are value, aptitude, interest. 3. Search information about interesting job and Compare it with other conditions. 4. Planning the most suitable career for oneself. These skills are taught in the program mentioned by means of short lecture, various experimential activities, and discussion. This training program consisted of 9 sessions, and each session lasted for 120 minutes. All activities of participants in this training program experience in group which are composed of 10-15 persons.