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정경희,이택기,손미권,송순욱,홍순선 대한약학회 2015 Archives of Pharmacal Research Vol.38 No.5
Severe acute pancreatitis (SAP), a commonnecroinflammatory disease initiated by the premature activationof digestive enzymes within the pancreatic acinarcells, is associated with significant morbidity and mortality. In this study, we investigated whether human bone marrowderivedclonal mesenchymal stem cells (hcMSCs), isolatedfrom human bone marrow aspirate according to our newlyestablished isolation protocol, have potential therapeuticeffects in SAP. SAP was induced by three intraperitoneal(i.p.) injections of cerulein (100 lg/kg) and sequentialLPS (10 mg/kg) in Sprague-Dawley (SD) rats. hcMSCs(1 9 106/head) were infused on 24 h after LPS injection viathe tail vein. The rats were sacrificed 3 days after infusion ofhcMSCs. We observed that infused hcMSCs reduced thelevels of serum amylase and lipase. Infused hcMSCs amelioratedacinar cell necrosis, pancreatic edema, and inflammatoryinfiltration. Also, infused hcMSCs decreased thelevel of malondialdehyde, and increased the levels of glutathioneperoxidase and superoxide dismutase. The numberof TUNEL positive acinar cells was reduced after hcMSCsinfusion. In addition, hcMSCs reduced the expression levelsof pro-inflammation mediators and cytokines, and increasedthe expression of SOX9 in SAP. Taken together, hcMSCscould effectively relieve injury of pancreatitis as a promisingtherapeutics for SAP.