http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
소호섭,한영희,이기영,임재욱,이부영,이윤형,최근원,박영두 한국원예학회 2009 Horticulture, Environment, and Biotechnology Vol.50 No.1
The objective of this study was to generate a pest-resistant Chrysanthemum morifolium by transforming crylAc gene, which has resistant to pests belonging to Noctuidae family that causes problems that damage to leaves and flowers during cultivation, into Chrysanthemum morifolium. For plant regeneration, standard type Jinba (JB) and Suhonochikara Yellow (SY) cultivars cultured on the medium supplemented with NAA 1.0 mg・L-1 and BA 2.0 mg・L-1 combination and spray type EuroYellow (EY) cultivar supplemented with NAA 1.0 mg・L-1 and BA 1.0 mg・L-1 combination showed the highest regeneration rates, respectively. When selectable reagents were added to selective medium for leaf segment culture, regeneration was inhibited on concentrations of above 0.5 mg・L-1, 5.0 mg・L-1 and 10.0 mg・L-1 hygromycin for SY, EY and JB cultivars, respectively, and 0.5 mg・L-1 phosphinothricin for all JB, SY and EY cultivars. When transgenic Chrysanthemum morifolium transformed by pGR-BT vector was analyzed by PCR, the expected size of 479 bp was observed on 29 JB lines, 13 SY lines and 5 EY lines. For pMJ-BT vector, 3 JB lines, 3 SY lines, and 1 EY line were selected as transformants. Finally, transformed lines selected by PCR analyses were inoculated with Spodoptera litura (tobacco cutworm) and showed that there was no larvae growth from 6 JB lines, 1 SY line and 1 EY line.