유변학적 시험에 의한 연고제제의 물성평가

성학모,도티 무이,오주석 한국인그리에학회 2019 한국인그리에학회지 Vol.1 No.1

Purpose: Ointment type products are mainly viscous, semi-solid and high viscosity products such as vaseline and lanolin. If there is a large physical resistance during use, or if the material is aggregated or separated, it will not only lower the quality satisfaction but also reduce the stability of the product's efficacy. In this study, we conducted a study to confirm whether rheological test analysis is valid as a performance index of ointment. Method: 3 kinds of ointment containing vaseline were treated with electron beam at 0, 10, 15, 20, and 30 kGy. The viscoelastic properties (G′, G″, η*) were analyzed at 24 °C. and 0.1 rad/sec to 10.0 rad/sec using an ARS Rheometer P-200 (diameter 50 mm Fixture, Gap 1.0 mm). The sensory evaluation of the skin was also carried out, and the correlation with the rheological test results was analyzed. Results & Discuss: As a result of the rheological test of each composition according to the electron beam treatment, the deviation of test result in the sample was high, and when the physical force was applied, the partial separation of the components perhaps appeared to show the difference in physical properties. Also, G′(elasticity) of sample A decreased to 66%, while sample B increased to 158% as irradiation dose increased. And sample C decreased gradually up to 30%. G′and G″of each composition showed a non-Newtonian pattern with a gradual increase with increasing frequency but a decrease in viscosity. Specifically, G′values were 11.9%, 55.8% and 43.8% for samples(A, B and C) at the frequency of 0.1 rad/sec, and 36.4%, 23.4% and 8.3% for the 10.0 rad/sec appear. Sensory evaluation of each composition on the 5-point scale showed that the composition of A was 3.0, B was 3.15, and C was the highest at 4.35. The rheological evaluation condition that best corresponds to this scale is G′(10.0). This means that people prefer low elasticity products when measured at high frequencies. Conclusions: The rheometric method, which is a rheological property measurement method, is considered to be a valid method to measure the homogeneity of composition and the influence of electron beam treatment. When ointments are applied to human skin, it will show shear-thinning behavior at body temperature. Therefore, it will be meaningful to evaluate the rheological test at 37 ℃ in the future.

Lactobacillus casei Phage J1 Genome의 Cohesive End Site 염기배열

김영창,성학모,강현삼,Kim, Young-Chang,Seong, Hark-Mo,Gang, Hyeon-Sam 한국미생물학회 1994 미생물학회지 Vol.32 No.3

Lactobacillus casei에 감염하는 bacteriophage J1 게놈의 cohesive end site (cos)의 염기배열을 결정하였다. 또한 환형 cos와 선형 J1 DNA의 왼쪽 말단 염기배열을 비교한 결과 terminase가 절단하는 위치는 다음과 같았다. 5'- GGTCGGCC$\downarrow$ -3' 3'- $\uparrow$CCAGCCGG -5' J1 게놈의 cohesive end는 3' 말단이 돌출되어 있으며 8개의 뉴클레오티드로 이루어져 있고 G+C 함유율이 87.5%이었다. cos 부위는 선형 DNA의 왼쪽 5' 말단 뉴클레오티드의 위치를 +1로 정하였을 때 -33부터 +25까지 대칭이었다. 지금까지 보고된 phage들의 cos 부위 사이에 상동성은 발견되지 않았다. The nucleotide sequence of the cohesive end site (cos) of Lactobacillus casei phage J1 genome was determined. Comparison between the nucleotide sequences of the circular cos and the left end of the linear J1 DNA showed that the nicking sites of the terminase were as follows: 5'- GGTCGGCC$\downarrow$ -3' 3'- $\uparrow$CCAGCCGG -5' The cohesive single-stranded ends of J1 were found to be 3'-protruding and composed of 8 nucleotides. The mol% G + C of the cohesive ends was 87.5. The cos site shows dyad symmetry from -33 to + 25 bp if the 5' terminal nucleotide of the left end of the linear J1 DNA is numbered +1. No homology was found among the cos sites of phages reported so far.

크림의 발림성에 대한 관능평가와 유변학적 평가의 관계 연구

유성민,김지원,박혜란,성학모,오주석 한국인그리에학회 2020 한국인그리에학회지 Vol.2 No.1

This study reveals the close relation between sensory evaluation and rheological measurement about smoothness of creams. A rheological measurement has advantage over sensory evaluation because it gives robust and unchanging results while sensory evaluations cannot. Cosmetics gives satisfaction to users after it has been used. But during the usage of cosmetics, customers can be satisfactory if the smoothness of cosmetics catches the users mind. To test the smoothness of cosmetics large makers pay much to establish and maintain trained panels. But for a small and medium companies the expenditure for the panels are so large and unbearable. If we find a mechanical and unchanging method, it can be helpful to many small cosmetics companies. In this study we conducted sensory evaluation with university students and rheological measurement with rheometer. And we did regression analysis to connect the results of sensory evaluation and rheological measurements. It is concluded that rheological measurements can replace sensory evaluation of smoothness of creams.

혈장분획제제 제조공정에서 크로마토그래피 세척 검증을 위한 모델바이러스로서의 Porcine Parvovirus 정량

길태건,김원중,이동혁,강용,성학모,유시형,박순희,김인섭,Kil Tae Gun,Kim Won Jung,Lee Dong Hyuk,Kang Yong,Sung Hark Mo,Yoo Si Hyung,Park Sue-Nie,Kim In Seop 한국미생물학회 2005 미생물학회지 Vol.41 No.3

혈장분획제제 중 혈액응공인자제제와 일부 면역글로불린제제는 혈장에 존재하는 다양한 단백질로부터 유효한 단백성분만을 선택적으로 분리 정제하기 위해 크로마토그래피 방법을 사용하여 생산된다. 효율적인 세척(cleaning) 공정이 이루어지지 않는다면 크로마토그래피는 다양한 종류의 불순물뿐만 아니라 혈액 중 내재 또는 오염 가능성이 있는 위해인자가 오염될 가능성이 있다. 본 연구에서는 혈장분획제제 제조공정에 사용되는 크로마토그래피의 세척 공정에서 혈장유래 바이러스의 제거 및 불활화 공정의 검토 강화로 혈장분획제제의 안전성을 확보하기 위해 크로마토그래피 세척 검증 시스템을 구축하고자 하였다. 크로마토그래피 세척 공정 중 바이러스 제거 검증을 위해 혈장유래 바이러스 중 물리${\cdot}$화학적 처리에 가장 큰 저항성을 갖는 human parvovirus B19의 모델 바이러스의 porcine parvovirus(PPV)를 대상으로 real-time PCR 정량법을 확립하였다. PPV에 특이적인 primer를 선별하였으며 형광염료 SYBR Green I을 사용하여 PPV DNA를 정량하였다. 세포배양법에 의한 감염 역가와 비교한 결과 PCR 민감도는 1.5 $TCID_{50}/ml$이었다. 확립된 검증법의 신뢰성(reliability)을 보증하기 위해 실험법의 특이성(specificity), 재현성(reproducibility) 등을 검증하였다. 구축된 검증시스템을 thrombin 분리${\cdot}$정제를 위한 SP-Sepharose 양이온 크로마토그래피 공정과 factor VIII 분리${\cdot}$정제를 위한 Q-Sepharose 음이온 크로마토그래피 공정에 적용하여 크로마토그래피 세척 검증을 실시하고, 세척 검증 시스템의 적합성을 확인하였다. Chromatography has now been used successfully to provide the requisite purity for human plasma-derived biop-harmaceuticals such as coagulation factors and immunoglobulins. Recently, increasing attention has been focused on establishing efficient cleaning procedures to prevent potential contamination by microorganisms as well as carry-over contamination from batch to batch. The purpose of present study was to develop a cleaning validation system for the assurance of virus removal and/or inactivation during chromatography process. In order to establish an assay system for the validation of virus clearance during chromatography cleaning process, a quantitative real-time PCR method for porcine parvovirus(PPV) was developed, since PPV, a model virus for human parvovirus B19, has a high resistance to a range of physico-chemical treatment. Specific primers for amplification of PPV DNA was selected, and PPV DNA was quantified by use of SYBR Green I. The sensitivity of the assay was calculated to be 1.5 $TCID_{50}/ml$. The established real-time PCR assay was successfully applied to the validation of PPV removal and cleaning during SP-Sepharose cation chromatography for thrombin purification and Q-Sepharose anion chromatography for factor VIII purification. The comparative results obtained by real-time PCR assay and infectivity titrations suggested that the real-time PCR assay could be a useful method for chromatography cleaning validation and that it could have an additive effect on the interpretation and evaluation of virus clearance during the virus removal process.

사람 혈장으로부터 고순도 혈액응고 제9인자의 산업적 생산

강용(Yong Kang),최용운(Yong Woon Choi),성학모(Hark Mo Sung),손기환(Ki Whan Sohn),신정섭(Jeong Sup Shin),김인섭(In Seop Kim) 한국생물공학회 2008 KSBB Journal Vol.23 No.1