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게르마니움화합물이 암세포 증식 및 림파구에 미치는 영향 : MOLT-4, L1210, Sarcoma180와 림파구에 대하여 MOLT-4, L1210 and Sarcoma180
정용자,성영기 慶星大學校 1996 論文集 Vol.17 No.4
I could take carboxyethylgermanium sesquioxide from ASAI Germanium Institute. It was experimented in cytotoxicity on carcinoma cell : Sarcoma180,L1210 and Molt-4, and in mitogenecity of lymphocytes in vitro. in vitro WBC contents were observed by Wright's staining under microscope after injected it, and the spleen and tumor size were measured by weight. Molt-4 is a suspension culture derived from the peripheral blood of acute lymphoblastic leukemia patient. L1210 is lymphocytic mouse leukemia and grows in suspension cultures or spinner cultures. Sarcoma 180 was derived from Swiss Webster Sarcoma 180 ascites. These cell lines have been used extensively for routine screening programs of chemical agents and natural products for cytotoxicity activity. These are also utilized in preliminary testing for antitumor activity in cancer chemotherapy screening studies. In order to test in vivo cytotoxicity of germanium compound, BALB/C mice were used. Sarcoma 180 cells were harvested by brief treatment with trypsin-EDTA and then inoculated in abdominal cavities of mice by intraperitoneal transplantation of 2×?cell/mouse. Germanium-30 ㎕ or nomal saline were injected intraperitoneally once a day for 6 weeks. The survival of treated sarcoma mice and the growth of tumor were observed during 6 weeks. At sixth week, sarcoma mice were decapitated and tumors and spleens were weighted. Into each well (0.2ml capacity) of 10³, 5×10³,2×10⁴,?cells/well were inoculated at Day-0. After culture during various time interval, the absorbances measured by MTT. At Day-2 or Day-3 after culture, and 5×10³and 10⁴cells/well, we can take the appropriate value in L1210 and Molt-4. Sarcoma cells were show the lower value compared with the other cells(Fig 1,2,3) To estimate the effect of germanium compound as a mitogen to lymphocytes, the absorbance of human lymphocytes cultured in medium containing germanium compound for 3days was measured by MTT method. Germanium compound increased it significantly at the concentration of 10㎍ and 30㎍/ml. When germanium compound compared with concanavalin A, that has higher ability of mitogenecity than concanavalinA on human lymphocytes.(Fig.4,5) The direct cytotoxicity of germanium compound was estimated by comparing absorbaned to the control which was cultivated without germanium compound. Fig 6,7 show that the absorbances of Sarcoma 180, L1210 of MOLT-4 cultured in medium containing germanium compound was decreased dose-dependently. It means that germanium compound exhibits direct cytotoxicity. However in the case of Molt-4, the absorbance was lower than the other, respectively of administered dose. Following injection of germanium compound of 2mg/kg/day for 2 weeks, whole blood was obtained in vivo. the leukocytes stained with wright's staining solution compared with the control to be injected normal saline instead of germanium compound. As shown in table 1, there was increase in lymphocytes, basophils and monocytes. At long-term treatment, LMP% was increased a little, but the tumor weight decreased or increased on individuality.
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체위 변환과 좌압 측정을 통한 자세교정 앱 서비스의 개발
최정현,박준호,성영기,서재용,박준모 한국융합신호처리학회 2023 융합신호처리학회 논문지 (JISPS) Vol.24 No.1
일반적으로 잘못된 앉은 자세를 장시간 유지하는 것은 척추에 나쁜 영향을 미치는 것으로 널리 알려져 있다. 최근 여러 연구자들이 잘못된 앉은 자세와 척추질환의 인과관계에 관심을 두고 있으며, 척추질환 예방을 위해 앉거나 서는 자세의 변화를 정밀하게 측정할 수 있는 방법을 연구해왔다. 우리는 과거 연구에서 실시간 체위 변환 측정이 가능한 센서 디바이스를 개발하고 운동량 계산 알고리즘을 적용하여 실시간 자세 전환 측정 정확도를 향상시켰으며, 체위 변환 측정 센서의 정확도를 검증하였다. 본 연구에서는 좌압 측정을 통해 체중심의 변화를 고려한 자세 측정 및 분석 장치를 개발하였으며, 개발된 센서는 사용자의 시각적 피드백을 개선하여 자세 교정 훈련 정확도를 높이는 보조 도구로의 활용 가능성을 확인하였다.
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아세틸 아르소네이트의 면역세포와 암세포에 미치는 영향: L1210, Sarcoma 180, MOLT-4 등 사람과 생쥐에 대한 작용비교
정용자(Yong Za Chung),성영기(Yung Ki Sung) 대한약학회 1996 약학회지 Vol.40 No.5
Acetylarsonate was prepared for testing antitumor and immunological effects. It showed cytotoxicity directly on Sarcoma 180. L1210 and MOLT-4 by MTT assay. It did not seemed to trigger the mitosis of human lymphocytes in culture, but that showed the cytotoxicity with higher dose. The rosette formation and spleen weight of mouse which acetylarsonate was administered to for 2 weeks were increased. Furthermore, peripheral helper T- and cytotoxic/suppressor T-lymphocytes were increased in acetylarsonate-injected-mice significantly when it was estimated with simultaneous 2 color analysis using anti Lyt2-FITC and L3T4-PE monoclonal antibody by Flow cytometer.
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토끼의 각막 추출액 중 메치오닌엔케팔린 및 [ D - 알라 2 ] - 메치오닌엔케팔린아미드의 분해 및 안정화
이치호,신영희,전인구,이경진,성영기 한국약제학회 1994 Journal of Pharmaceutical Investigation Vol.24 No.1
In order to study systemic peptide delivery through the ocular route, the stabilities of methionine enkephalin (Met-Enk) and [D-ala²]-methionine enkephalinamide (YAGFM) in the corneal extracts of rabbits were investigated using reversed phase HPLC. Met-Enk was found to be hydrolyzed most rapidly in the corneal epithelium, but YAGFM was relatively stable. Aminopeptidases appeared to contribute over 60% to the degradation of Met-Enk and the degradation rate of Met-Enk followed the first order kinetics. The half-lives of Met-Enk in the extracts of the corneal epithelium and endothelium were 36 and 673 min, respectively. From the effects of enzyme inhibitors, it was found that the application of the mixture of amastatin, thimerosal and EDTA was very useful for the inhibition of peptide degradation.
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