히스톤 단백과 핵과의 결합

정용자 慶星大學校 1988 論文集 Vol.9 No.1

HISTONE은 CYTOPLASM에서 합성되어 진핵 세포의 염색체와 결합하여 있다. 그러나 이들 PROTEIN의 핵막을 통한 이동에 관해서는 거의 알려져 있지 않다. HeLa CELL에서 합성후 곧 이행됨을 나타냈으며 (1967 ROBGIN등), HISTONE은 그것이 양이돈 CHARGE를 가지고 있디 때문인지, 아니면 특별한 TRANSPORT SYSTEM이 관여함으로써 이행하는 것인지을 알기 위해서 RAT LIVER에서 핵을 분리하여 분리한 핵과 ??C-HISTONE을 결합시킨후 (BINDING), 또다시 그 BINDING된것을 SDS 전기영동에 의해 해리되지 않도록 CROSS-LINKER를 사용해서 (DSP) 안정결합(CROSS LINKING)을 시킨 후 ELECTROPHORESIS와 RADIOGRAM에 의해 안정결합(CROSS LINKING)을 시킨후 ELECTROPHORESIS와 RADIOGRAM에 의해 함유 PROTEIN을 CECK함으로서 우선 HISTONE과 NUCLEL가 결합되는 여부의 실험을 행함. 실험결과 : HISTONE과 NUCLEI는 INCUBATION PERIOD와 농도에 따라 결합속도와 범위에 관여하며, 온도에 따라 다르다. HISTONE과, NUCLEISMS DITHIOBIS(SUCCINIMIDYL PROPIONATE)의 존재하에서 핵 PROTEIN과 결합하여 RADIOGRAM에서 BINGING BAND가 나타났음. Interactions between protein components of rat liver nuclei and histone were investigated by using cross-linking reagent, Dithiobis (succinimidyl propionate). The rate and extent of binding was a function of the incubation period and the concentration of histone and nuclei, but independent of the temperature.

식품에 관련하는 미생물상의 위해 및 식품 위생상의 문제점

鄭容子 부산여자대학 1979 釜山女子專門大學 論文集 Vol.1 No.-

The microbial multiplication & the contamination of the food have greatly affected human health. Therefore the quality & freshness of the food become an issue in the epidemiologic problem & the food habits and the protection of the consumer health in consideration of the many conditions participating the growth & exsistence of the microorganism, and the production of the toxin. The major methods of the contamination prevention in food microorganism are as follows. Salmonella group: Block the contamin ation route from the animal to the man because the cycle is among man, animal and environment. Shigella: Block the contamination route from stool because the man is only carrier. Bacterium coli neapolitanum: preventive measure of food contanination & control of the microbial multipication of the food. Vibrio cholera: It has direct connection, and ha ve relation to preventive knowledge. Vibrio parahaemolyticus: Sea food is causative food, its prevention is forbidden vibrio from multiplication in sea food. Clostridium group: Stored food, especially the food of storage life in household affairs is causative food. Its prevention is heating & cooking, and storage required rapid cooking after heating. Fungus & mycotoxin: Fodder need prevention of production of mycotoxin and its establish the method in its harvest, storage and transportation parasites: Best prevention knows whether parasites contamination slaughtering animals. Transmission of drug tolerance intestinal bacteria: A used antibiotics in fodder has possibility that drug tolerance spread from animal to man. Therefore abuse of antibiotics should prohibit in animals. The general run of people need the distribution of information on matters of environ mental hygiene for examples ① microbial multiplication & toxin production due to stability dealing with food , food manufacture, processing & storagc. ② the microbiologic injury in relation to food & countermeasure of prevention. Sanitary distribution of food needs strict hygiene inspection.

식품에 관련하는 미생물상의 위해 및 식품 위생상의 문제점

鄭容子 亞成出版社 1979 釜山女子大學 論文集 Vol.1 No.-

The microbial multiplication & the contamination of the food have greatly affected human health. Therefore the quality & freshness of the food become an issue in the epidemiologic problem & the food habits and the protection of the consumer health in consideration of the many conditions participating the growth & exsistence of the microorganism, and the production of the toxin. The major methods of the contamination prevention in food microorganism are as follows. Salmonella group: Block the contamin ation route from the animal to the man because the cycle is among man, animal and environment. Shigella: Block the contamination route from stool because the man is only carrier. Bacterium coli neapolitanum: preventive measure of food contanination & control of the microbial multipication of the food. Vibrio cholera: It has direct connection, and ha ve relation to preventive knowledge. Vibrio parahaemolyticus: Sea food is causative food, its prevention is forbidden vibrio from multiplication in sea food. Clostridium group: Stored food, especially the food of storage life in household affairs is causative food. Its prevention is heating & cooking, and storage required rapid cooking after heating. Fungus & mycotoxin: Fodder need prevention of production of mycotoxin and its establish the method in its harvest, storage and transportation parasites: Best prevention knows whether parasites contamination slaughtering animals. Transmission of drug tolerance intestinal bacteria: A used antibiotics in fodder has possibility that drug tolerance spread from animal to man. Therefore abuse of antibiotics should prohibit in animals. The general run of people need the distribution of information on matters of environ mental hygiene for examples ① microbial multiplication & toxin production due to stability dealing with food , food manufacture, processing & storagc. ② the microbiologic injury in relation to food & countermeasure of prevention. Sanitary distribution of food needs strict hygiene inspection.

Antitumor and Immunological Effects of Bovine Thymic Extract

정용자,홍기태,정현옥,Chung, Yong Za,Hong, Gi Tae,Jung, Hyun Ok Korean College Of Clinical Pharmacy 1992 한국임상약학회지 Vol.2 No.1

Thymic extract showed antitumor effect to sarcoma mice with higher dose$(200{\mu}g/mouse/day$ i.p., 4weeks) but not with low dose$(5{\mu}g/mouse/day$ i.p., 6 weeks). Direct cytotoxicities were exhibited against sarcoma 180, L1210 and MOLT-4 by MTT assay. The spleen weight of mice were increased but the number of circulating lymphocytes were not increased after long-term(2 weeks) administration of thymic extract. Evaluating the mitogenesis by MTT assay. $\%$ absorbance of human lymphocytes was not increased by thymic extract. Cell cycle statistics of S phase and $G_2/M$ phase was not increased in the presence of that by PI staining. The formation of rosette was induced, irrespectively of exposure time short-term(l hour) and long-term(2 weeks). The population of mouse blood T-cell to bind Lyt2-antimonoclonal antibody and to $L_2T_4$ were increased after administration of thymic extract$(2-200{\mu}g/mouse/day)$. From the above results, it is suggested that thymic extract exerts antitumor activity by stimulating T cells to differeniate in vivo but not in vitro.

소 흉선 추출물의 암세포 증식 및 림프구에 미치는 영향

정용자,홍기태,정현옥 한국임상약학회 1992 한국임상약학회지 Vol.2 No.1

Thymic extract showed antitumor effect to sarcoma mice with higher dose i.p., 4weeks) but not with low dose i.p., 6 weeks). Direct cytotoxicities were exhibited against sarcoma 180, L1210 and MOLT-4 by MTT assay. The spleen weight of mice were increased but the number of circulating lymphocytes were not increased after long-term(2 weeks) administration of thymic extract. Evaluating the mitogenesis by MTT assay. absorbance of human lymphocytes was not increased by thymic extract. Cell cycle statistics of S phase and phase was not increased in the presence of that by PI staining. The formation of rosette was induced, irrespectively of exposure time short-term(l hour) and long-term(2 weeks). The population of mouse blood T-cell to bind Lyt2-antimonoclonal antibody and to were increased after administration of thymic extract. From the above results, it is suggested that thymic extract exerts antitumor activity by stimulating T cells to differeniate in vivo but not in vitro.

수종 식물들의 Hepatoma cell 증식 저해작용

정용자,이은주 한국임상약학회 1999 한국임상약학회지 Vol.9 No.2

고추 부위별추출물에 의한 종양세포의 세포사유도 - Hepatoma 세포와 MCE-7 세포 -

정용자 대한약학회 2003 약학회지 Vol.47 No.2

Under the active search for biologically active novel agents for cancer prevention and treatment, some agents have been found from plants which are easily available. Our previous research on them revealed that C. annuum L. var. angulosum Mill have high antiproliferating effect on cancer cells. However, it has not been known whether the anticancer efficacy is different according to each part of C. annuum L. var. angulosum Mill or whether it can be changed by timing of harvest or solvent for extraction. Thus we compared the efficacy of each part of C. annuum L. var. angulosum Mill and assessed how much difference in the efficacy can be made according to the time of harvest or solvents for extraction. We observed the morphologic change and apoptosis 48 hr after treatment with the extract of each part of C. annuum L. var. angulosum Mill in MCF-7 mammary gland adenocarcinoma cells and human hepatoma cells. We also counted cancer cells by trypan blue method and MTT method to check the cytotoxicity. The leaf extract showed the highest anticancer effect among all the parts of C. annuum L. var. angulosum Mill; 50% and 70% reduction in the number of cancer cells was observed at 25 $\mu\textrm{g}$/mι and 50 $\mu\textrm{g}$/mι, respectively. It was more than 2 times as potent as 5-fluorouracil (5-FU). We found chromosomal fragmentation, clumping, and destuction by PI staining, and DNA fragmentation by electrophoresis. In conclusion, this study suggests that leaf extraction using water as solvent has the highest antiproliferative and apoptotic activity in cancer cells compared with other parts of extraction.

대두 saponin의 성질 Ⅰ

정용자 慶星大學校 1986 부산수산대학 논문집 Vol.7 No.2

大豆(Glycine max. MERRILL, Lequminosae)는 전통적으로 많은 식품에 이용되고 있다. 일반성분으로 steroids, flavonoids, glyceolin, 외에도 필수인 지질, stigmasterol과 saponin등이 함유되어 있고, saponin ⅠㆍⅡㆍⅢㆍ은 혈중 과산화지질의 생성을 억제하므로 성인병에 방제로서의 개발이 기대된다는 보고와 더불어 大豆saponin의 性質및 生理作理에 미치는 영향을 연구하고자 우선 C.M.C.(the critical micelle concentration), bovine serum albumin과의 결합 및 Dipalmitoyl L-α-phosphatidyl choline(DPPC) liposome의 성질에 미치는 영향을 관찰하고 이에 보고하고자 한다. CMC, sap.-albumin binding and phase transition temperature of soyasaponin are following. 1. The critical micelle concentration(CMC) of soyasaponin(sap. ) was determined by fluorometry and surface tensiometry. The CMC values given from these two methods are 8×10??M and 7.4×10??M, respectively. The surface exces of the sap. (Γ) and the area (A) occupied by a sap. molecule at the monolayer absorbed at air and water interface were calculated employing Gibbs absorption equation. The values are Γ×10??=1.205mole/㎠, and A=132.7A??. The presence of buffer increased the surface activity of the sap, and decreased the CMC, the surface tension at the CMC. 2. The binding of soyasaponin to Bovine serum albumin(BSA) was studied by the fluorescence method, 1-anilinonaphthalene-8sulfonate(ANS) as fluorescent probes. The evaluations of the nature and degree of association of sap.-albumin complex are following: the number of binding sites of BSA for sap. is 2.2, and binding constant is 8.4×10₃M??. Competitive bindings between sap. and probe were observed. All of these are affected by pH and metal ions. 3. The differential scanning thermograms of the DPPC liposomes in the presence of soyasaponin showed that the phase transition temperature of the liposomes was significantly lowed and broadened by 0.1mM sap. This was ascribed to the possibility that incorporation of sap. into the lipid bilayer reduced the cooperativity unit of the layer and decreased the phase transition temperature. The order of lowering in the phase transition temperature by sap. were approximately indentical to the order of the pharmacological activity of sap.

Capsicum annuum L var angulosum Mill의 가식부위에 따른 항종양효과

정용자,이원섭,이병황 한국생명과학회 2002 한국생명과학회 심포지움 Vol.35 No.-

식용식물들이 MCF-7 cell의 chromosome형태에 미치는 영향

정용자,곽수영 한국생명과학회 2002 생명과학회지 Vol.12 No.3

Capsicum annuum, Lactuca dentata Makino., Equisetum arvense, 들의 MCF-7 mammary gland adenocarcinoma cell line에서의 항증식효과는 현미경관찰과 세포수 관찰에서 얻은 결과와 거의 동일하게 Chromosome의 condensation, PI염색에서의 핵 변화, 전기영동과 흡광도법을 이용하여 관찰된 세포의 DNA fragmentation 결과에서도 시료에 따른 항종양증식억제효과가 유사하게 관찰되었다. 즉 그 결과는 Capsicum annuum L. var. angulosum Mill, leaves 추출시료틀 첨가한 경우에는 세포증식을 위한 탁상조차도 제대로 되지 않음이 확인되었고, 세포변형과 apoptosis의 일부가 2∼3일후 부터 관찰되었다. chromosome 농축 현상도 시료첨가 37시간에 관찰되었고, PI염색법에서도 시료첨가 3시간에 핵 농축현상이 관찰되기 시작하였다. 그 핵 농축현상을 배양시간에 따라 농축현상이 더해가다가 48시간에 파쇄현상을 보였다. 이 실험결과에서 Capsicum annuum L. var. angulosum Mill 추출물을 첨가한 배지의 MCF-7cell은 apoptosis의 형태학적 특징인 chromosome의 degradation과 PI염색법에서 핵의 농축이 발견되었으며 48시간 위 핵파편들을 관찰할 수 있었다. 전기영동을 이용한 세포의 DNA fragmentation관찰에서도 염색체 DNA의 관찰이 거의 확인되지 않았다. 260 nm 흡광도측정으로 부유층의 흡광도를 측정한 결과에서도 Capsicum annuum L. var. angulosum Mill은 배양초기부터 부유층의 흡광도가 높게 나타났다. 이들의 결과에서 Capsicum annuum L. var. angulosum Mill은 MCF-7 cell의 세포사를 유도하여 강한 항암효과를 나타내는 것을 확인할 수 있었다. Equisetum arvense L와 Lactuca dentata Makino. var. flaviflora Makino에서도 배양 2∼3일에 세포성장이 억제되고 2일에 chromosome의 condensation과 degradation이 관찰되었다. 이상의 결과로부터 Capsicum annuum, Lactuca dentata Makino, Equisetum arvense 추출물에서 모두 항암효과를 관찰할 수 있었다. Equisetum arvense L. and Lactuca dentata Makino. var. Flaviflora Makino. of samples relatively showed anticancer effects on MCF-7 mammary gland adenocarcinoma cell. The most active plant among the samples was Capsicum annuum L. var angulosum Mill. We studied that MCF-7 cells were changing chromosomal morphology and apoptosis on these samples. Capsicum annuum L. var. angulosum Mill. of samples relatively showed good anticancer effects. The cells became vague after 2 days and then destroyed. The supernatant of the cells including medium was measured by UV absorbance. The results showed that Capsicum annuum L. var. angulosum Mill also exerted high level. We also used electrophoresis in order to observe apoptic characterization of DNA fragmentation. The cells treated with Capsicum annuum L. var. angulosum Mill showed the apoptotic characterization. The chromosome of the cells were observed on those samples. The cells treated with Capsicum annuum L. var. angulosum Mill among them were shown the fastest changes. The cells were aggregated and destroyed by treatment with some edible plants. Especially, the case of Capsicum annuum L. var. angulosum Mill, it led MCF-7 cell to apoptosis faster than others. And we can observe chromosomal changes and dispersion by PI staining. These results showed that each sample exerted anticancer effects on MCF-7 cells. Especially Capsicum annuum L. var. angulosum Miff exerted significant anticancer effects.