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전창기,석도방랑 ( C . G . Jeon ) 한국축산학회 1974 한국축산학회지 Vol.16 No.4
This experiment was studied of the peritoneal fluid when it was used for culture fluid of fertilized ova of rabbits and it was cultivated in vitro. The peritoneal fluid also can be gained much from the superovulated rabbits. The results were as follow. 1. When 2, 4, 8 cell stage ova of rabbit were cultivated in peritoneal fluid at 37℃ for 48 hours each ova was developed as much as 78.1%, 83.3% and 91.7%, and these records were superior to the useing of blood serum. 2. When 2 cell stage ova of rabbit was cultivated in peritoneal fluid, 88% of ova were rapidly growth for morula stage and 24% became blastcyst stage. These records are nearly same to the records in blood serum. At these points, the rabbit peritoneal fluid can be used effectively for ova culture fluid.
전창기,석도방랑 ( C . G . Jeon ) 한국축산학회 1974 한국축산학회지 Vol.16 No.4
This experiment was studied on the times of fetus-death during the gestation period of superovulated mows, and inspectation of implantation and living fetus number and the implantation points were done after 10, 12, 14, 16, 18 days from gestation. The results are as follows: 1. The coputaion rate was 68.1% (47/69) and the gestation rate was 68.1% (32/47) of superovulated mouse. 2. The average number of implantation was resulted like these, the 10 days after was 20.8, 12days after 18. 2, 14 clays after 17.1, 16 days after 17.0 and 18 days after 19.0, while the average number of living fetus were 10 days after 20.8 (100%: living fetus number/implantation number), 12 days after 16.3 (89.9%), 14 days after16.9 (98.3%), 16 days after 10.7 (62.7%) and 18 days after 10.2 (53.7%), and living fetus number was rapidly decreased from 16 days over. 3. The litter size of superovulated mouse was average 6.9 heads and did not over the control plot of which number is aveage 9.7 heads.