학술발표(學術發表) 연제(演題) 및 초록(抄錄) 제삼부(第三部): 역학(疫學),해부(解剖) : 23. 한국재래산양의 태아 및 신생아의 폐 발달에 관하여

서득록,이국천,이순선,김종섭 한국가축위생학회 1996 학술대회논문집 Vol.1996 No.-

한국 재래산양의 태아 및 신생아의 폐 발달에 관하여

서득록 ( Deuk Lok Seo ),이국천 ( Kuk Cheon Lee ),이순선 ( Soon Sun Lee ),김종섭 ( Chong Sup Kim ) 한국가축위생학회 1997 韓國家畜衛生學會誌 Vol.20 No.1

The morphological development of lungs in fetuses of 60, 90 and 120 days of gestation and neonates of Korean native goats was investigated by light, scanning and transmission electron mictroscope. The results were summarized as follows; 1. Gross findings: In the 60-days-old fetus, the lung was developed and differentiated into six lobes. 2. Light microscopic findings: The gland-like bronchioles were formed in loose mesenchyme at 60 days of gestation and the bronchial wall contained smooth muscles. The loose mesenchyme had been replaced by compact parenchymal tissue at 90 days of gestation and the cartilage plates appeared in bronchial wall which contained blood vessels, submucosal glands and smooth muscles. The lung parenchyma consisted of a fine network of alveoli at 120 days of gestation and the bronchial wall contained well-developed blood vessels, submucosal glands, cartilage plates and smooth muscles. In neonates, the lung tissue was similar to the mature lung tissue and the bronchial wall contained well developed cartilage plates. 3. Scanning electron microscopic findings: The epithelial cells lining the tubules were composed of cuboidal or columnar at 60 days of gestation and the epithelial cells lining the large airways were often ciliated; some were covered with stubby microvilli. The epithelial cells lining the canals were cuboidal at 90 days of gestation and the epithelial cells lining the bronchioles were ciliated cells or nonciliated(clara) cells, The clara cells contained row microvilli. The alvealor development of this stage was rapidly progressed; the subdivision of canals by alveolar crests and assosiated wall attenuation resulted alveoli at 120 days of gestation and the respiratory bronchioles were lined by ciliated or nonciliated epithelial cells. In neonates, the epithelial cells lining the alveolar walls were mainly covered with pneumocyte type Ⅰ; Some were covered with pneumocyte type Ⅱ. 4. Transmission electron microscopic findings: The epithelial cells lining the tubules were adhered with tight junction at apical borders of the adjacent cells at 60 days of gestation, which contained few organells and glycogen. The epithelial cells lining the canals were composed mostly of cuboidal cell at 90 days of gestation and the epithelial cells lining of the bronchioles were ciliated of nonciliated cell, which contained few organelles and abundant glycogen. The epithelial cells lining the alveolar walls were composed of pneumocyte type I and a few pneumocyte type Ⅱ at 120 days of gestation. The epithelial cells lining of the bronchioles were ciliated or nonciliated cells. In neonates, pneumocyte type I was observed as flat and thin cytoplasmic extension in shape. Otherwise, pneumocyte type Ⅱ was observed as cuboidal type with apical microvilli and contained osmiophillic lamellar inclusion bodies. Putting these various experiment results together, the lung development was slowly progressed at early stage, which was rapidly progressed in the late stage of gestation.

경남(慶南) 북부지역(北部地域)에서 도축(屠畜)된 한우(韓牛)의 간질(肝蛭) 감염율(感染率)과 혈액성장(血液性壯)의 변화(變化)에 관한 연구(硏究)

서득록 ( Deuk Lok Seo ),이국천 ( Kuk Cheon Lee ),이순선 ( Soon Sun Lee ),김종수 ( Jong Shu Kim ),김충희 ( Chung Hee Kim ) 한국가축위생학회 1995 韓國家畜衛生學會誌 Vol.18 No.2

An abattoir survey was carried out on 300 Korean native cattles reared and slaughtered in North Kyeongnam area to determine the prevalence of Fasciola hepatica infections. The average prevalence of fascioliasis was 46.4%; the infection rate had a tendency to increase with age. Some hematological and biochemical indices were assayed in Korean native cattle naturally harbouring Fasciola hepatica infection and compared with uninfection control. Affected animal revealed reduction total erythrocyte count and homoglobin, which was consistent in total leucocyte count and packed cell volume. Infected cattle had lower level of serum total protein and albumin, was consistent serum calcium and glucose indices, had higher levels of sGOT, sGPT and choresterol indices.

경남지방의 도태우에 불임과 관련된 난소의 형태학적 관찰 1. 난포와 황체의 출현에 대하여

양재훈,표병민,서득록,고필옥,강정부,곽수동,김종섭 한국임상수의학회 2002 한국임상수의학회지 Vol.19 No.2

Ovaries from total 192 slaughtered cows, 154 Korean native cows and 38 dairy cows were collected during the slaughtering process in Kimhae, Changyoung and Yangsan abattoirs in Kyungnam province from January 2001 to January 2002. Rates of pregnant and non-pregnant and ovarian findings were invested. Rates of pregnant cows in 192 slaughtered cows were 12.5% (24 cows) and in difference of cow breeds, 11.0% (17 cows) in 154 Korean native cows and 18.4% (7 cows) in 38 dairy cows from total 192 cows, respectively. Ages of fetuses in pregnant Korean native cows were mostly less than 4 months and ages of fetuses in dairy cows were mostly about 7-8 months. Cows which each diameter of follicles and corpus luteums in same cow was more than 5-6 mm in diameter were 69.8% (134 cows) in total 192 slaughtered cows and in difference of cow breeds, 64.7% (11 cows) in 17 Korean native cows and 57.1% (4 cows) in 7 dairy cows. Mean diameter of foliicles and corpus luteums in Korean native cows are 13.7±5.6×11.2±4.6mm and 17.5±4.6×14.6 ±4.0 mm in non-pregnat cows, and are 11.0±4.8×9.1±2.6mm and 21.2±2.9×18.3±2.7mm in pregnant cows, respectively. Mean diameter of follicles and corpus luteums in dairy cows are 15.8±7.1×14.3±6.0 mm and 20.3±5.9× 16.9±5.8mm in non-pregnant cows, and are 10.1±3.0×9.2±2.3mm and 23.0±1.7×20.1±1.3mm in pregnant cows, respectivley. The above findings indicate that the co-appearance rate of follicles and corpus luteums in same cows are higher in both pregnant and non-pregnant cows. Compared in pregnant and non-pregnant cow ovaries, mean size of follicles are smaller in pregnant cows but size of corpus luteums are more larger in pregnant cows than in non-pregnant cows. Correlation of the follicle size (Y) and corpus luteum size (X) in same cows developed each other in inversive size. Those correlative formulas appeared to be Y=-0.2022X+17.175 in Korean native cows and Y=-0.5754 X+24.153 in dairy cows.

Mice에서 D-galactosamine과 lipolysaccharide의 유도에 의한 간장의 apoptosis

곽수동,김종섭,강정부,고필옥,서득록,양재훈,Kwak, Soo-dong,Kim, Chong-sup,Kang, Chung-boo,Koh, Phil-ok,Seo, Deuk-Iok,Yang, Je-hoon 대한수의학회 2000 大韓獸醫學會誌 Vol.40 No.2

Experimental induction of apoptosis by bacterial lipopolysacchardie is useful for understanding the role of apoptosis cell death in clinical endotoxin shock or septic shock. Thirty three mice were injected intraperitoneally with D-galactosamine (20mg) and lipopolysac-charide ($5{\mu}g$) per mouse. Five to eight mice per each experimental group were sacrificed at 6, 12, 24, 48 and 72 hrs post administration. The cells with apoptotic bodies in H-E stained sections were investigated histologically. Development of the apoptotic bodies in livers was observed in 11 of 33 mice (33.3%). These cells were diffusely or collectively appeared only in liver but not observed in kidney, thymus and spleen. Mean percentage of the cells with apoptotic bodies in the livers were 0.32, 4.34 and 5.50% respectively at 6, 12, and 24 hrs post administration. But percentage of these apoptotic cells were fairly less in 48 and 72 hrs post administration. The percentages of cells with 3 to 9 apoptotic bodies per cell were 70~90% of all apoptotic cells. The cells with more apoptotic bodies than limit number at 12 to 72 hrs post administration were belived to be necrosed. The percentage of positive cells by TUTNEL methods were 0.00~0.08, 0.00~052, 1.63~4.18, 12.41~20.21 respectively at control, 6, 12 and 24 and less than 0.01% at 48 and 72 hrs. The above results suggest that development of liver cell apoptosis by lipopolysaccharide ($5{\mu}g$) and D-glatosamine (20mg) was less at 6 hrs and markedly increased at 12 to 24 hrs and then was fairly less at 48 and 72 hrs post administration.

Diethylnitrosamine을 투여한 rat 간장의 tumorigenesis에 관하여 3. 간장조직의 전자현미경적 관찰

곽수동,김종섭,고필옥,양재훈,서득록,Kwak, Soo-dong,Kim, Chong-sup,Koh, Phil-ok,Yang, Je-hoon,Seo, Deuk-lok 대한수의학회 1999 大韓獸醫學會誌 Vol.39 No.6

The study was designated to investigate the electron microscopic findings following diethylnitrosamine (DEN) treatment in rats. Forty four male (Srague Dawley) rats were continuously given water containing 0.01% DEN for 13 weeks and livers of five rats with more tumor lesions at 16 and 17 weeks after initial treatment were used as EM materials. In transmission electron microscopic findings, most small-sized hepatocytes were active cells containing large mount of organelles, but light (pale staining) hepatocytes among small-sized hepatocytes were injured cells containg disorganized organelles. Tumor cells among small-sized hepatocytes were irregularly arranged and have pleomorphic nuclei containing electron dense chromatin but the organelles in cytoplasm were swelled. Large-sized hepatocytes were active cells with condensed chromatin but the cytoplasm of these cells were pale due to be injured and dilated organelles. Dark hepatocytes were apoptotic cells with homogenous pyknotic nuclei and cytoplasm, and the cytoplasm of these cells contained dilated smooth endoplasmic reticulum (sER) but these sER were non-vesiculated. Cholangiocarninoma cells were crowded and were pale by far less number of organelles in cytoplasm and nuclei. In scanning electron microscopic findings, the lumens of portal veins, bile canaliculi, bile ductules, bile ducts and sinusoids were dilated and have irregular folded inner surface by protruded parenchyma.

경남지방의 도태우에 불임과 관련된 난소의 형태학적 관찰 : 2. 난소낭종의 발생과 낭종형태에 대하여 2. Incidences and Morphological Findings of Ovarian Cysts

곽수동,표병민,양재훈,김철호,고필옥,강정부,서득록 한국임상수의학회 2002 한국임상수의학회지 Vol.19 No.2

Ovaries from total 192 slaughtered cows(154 Korean native cows and 38 Holstein cows) were collected during the slaughtering process in Kimhae, Changyoung and Yangsan abattoirs in Kyungnam province from January 2001 to January 2002. In order to investigate incidence of the ovarian cysts, anatomical, histological observations were performed and also TUNEL methods and PCNA antibody by immunohistochemical methods for diagnostic accuracy of cysts in a few ovaries were applied. Apoptotic positive cells by TUNEL method appeared not or a few in cystic walls but appeared more number in normal large follicular walls and the proliferative positive cells by PCNA antibody appeared numerous in normal large follicular walls but not or a few in cystic walls. The incident rates of ovarian cysts were 19.5% in Korean native cows and 18.4% in Holstein cows. The incident rates of ovarian cysts in Holstein cows were lower than that of Koran native cows. The incident rates of follicular cysts and luteal cysts in Korean native cows were 11.7% and 7.8% respectively. The incident rates of follicular cysts and luteal cysts in Holstein cows were 10.5% and 7.9%, respectively. Higher incidence proportions of ovarian cysts according to seasons in Korean native cows were ordered as spring (29.8%), autumn (21.4%) winter (14.3%) and summer (6.7%). Rates of cows with single cyst and multiple cysts were 63.3%(19 heads /30 heads) and 36.7%(11 heads/30 heads) in 30 cystic Korean native cows, respectively. Cystic cows with corpus luteums were 50.0%(15 heads) in 30 Korean native cows and 42.9%(3 heads) in 7 dairy cows, respectively. Among 15 cystic Korean native cows with corpus luteums, rates of cows with single corpus luteum were 66.7%(10 heads) and rates of multiple corpus luteum were 33.3%(5 heads ), respectively. The average diameter of cysts and corpus luteums in cystic ovaries were 21.0×17.1 ㎜ and 18.1×13.8 ㎜ in 30 Korean native cows and 20.6×17.7 ㎜ and 19.3×14.9 ㎜ in 7 Holstein cows, respectively. So the average sizes of cysts in cystic ovaries were larger than those of corpus luteums.