Evaluating Feasibility of Bacillus sp. Application as a Biofertilizer for Stimulating Rice Productivity and Mitigating Greenhouse Gas Emissions in a Paddy Soil

Sang Yoon Kim(김상윤),Chang Hoon Lee(이창훈),Mee Kyung Sang(상미경),Hang-Yeon Weon(원항연),Jaekyeong Song(송재경) 한국토양비료학회 2016 한국토양비료학회 학술발표회 초록집 Vol.2016 No.10

Effect of Bacillus velezensis GH1-13 on Potato Productivity and Soil Bacterial Communities under Different Fertilization Regimes

Sang Yoon Kim(김상윤),Mee Kyung Sang(상미경),Hang-Yeon Weon(원항연),Jaekyeong Song(송재경) 한국토양비료학회 2017 한국토양비료학회 학술발표회 초록집 Vol.2017 No.10

고추 식물의 건조 스트레스 완화를 위한 미생물 선발

김상태(Sang Tae Kim),유성제(Sung-Je Yoo),송재경(Jaekyeong Song),원항연(Hang-Yeon Weon),상미경(Mee Kyung Sang) 한국식물병리학회 2019 식물병연구 Vol.25 No.3

Drought stress is considered as one of major abiotic stresses; it leads to reduce plant growth and crop productivity. In this study, we selected bacterial strains for alleviating drought stress in chili pepper plants. As drought-tolerant bacteria, 28 among 447 strains were pre-selected by in vitro assays including growth in drought condition with polyethylene glycol and plant growth-promoting traits including production of 1-aminocyclopropane-1-carboxylate deaminase, indole-3-acetic acid and exopolysaccharide. Sequentially, 7 among pre-selected 28 strains were screened based on relative water content (RWC); GLC02 and KJ40, among seven strains were finally selected by RWC and malondialdehyde (MDA) in planta trials under an artificial drought condition by polyethylene glycol solution. Two strains GLC02 and KJ40 reduced drought stress in a natural drought condition as well as an artificial condition. Strains GLC02 or KJ40 increased shoot fresh weight, chlorophyll and stomatal conductance while they decreased MDA in chili pepper plants under a natural drought condition. However, two strains did not show biocontrol activity against diseases caused by Phytophthora capsici and Xanthomonas campestris pv. vesicatoria in chili pepper plants. Taken together, strains GLC02 or KJ40 can be used as bio-fertilizer for alleviation of drought stress in chili pepper plants.

근권미생물에 의한 식물의 생물·환경적 복합 스트레스 내성 유도

유성제,상미경,Yoo, Sung-Je,Sang, Mee Kyung 한국식물병리학회 2017 식물병연구 Vol.23 No.2

식물은 재배기간 동안 세균, 진균, 바이러스 등의 생물 스트레스뿐만 아니라 고온, 염, 건조 등 다양한 환경 스트레스에도 노출되어 왔다. 최근에는 기후 이상현상으로 인하여 환경 스트레스의 빈도 및 강도가 불규칙적으로 증가하고 있으며 이로 인해 병원균의 생장과 영향도 변화하여 생물과 환경의 복합 스트레스가 식물 재배에 큰 영향을 주고 있다. 유용미생물을 이용한 식물의 저항성 유도는 다양한 생물과 환경 스트레스로부터 식물을 보호하는 데 도움을 주며, 이러한 스트레스에 대한 피해를 감소시킬 수 있는 가능성을 열어 주었다. 본 리뷰에서는 식물의 생물과 환경 스트레스에 대한 피해를 감소시키는 데 영향을 주는 미생물의 결정인자에 대해 기술하였으며 미생물 결정인자에 의해 유도되는 식물 신호전달 체계 변화에 대해 기술하였다. 또한 복합 스트레스 경감을 위한 미생물의 역할과 연구 방향에 대해 기술하였다. 이 리뷰를 통해 변화하는 환경에 대비하기 위해서 다양한 방안을 마련하고 있는 농민들에게 도움이 되기를 바라며, 실제 유용미생물 연구가 식물 재배 중 발생할 수 있는 다양한 스트레스에 따른 농가 피해를 감소시킬 효과적 대응 방안으로 이어지길 바란다. Recently, global warming and drastic climate change are the greatest threat to the world. The climate change can affect plant productivity by reducing plant adaptation to diverse environments including frequent high temperature; worsen drought condition and increased pathogen transmission and infection. Plants have to survive in this condition with a variety of biotic (pathogen/pest attack) and abiotic stress (salt, high/low temperature, drought). Plants can interact with beneficial microbes including plant growth-promoting rhizobacteria, which help plant mitigate biotic and abiotic stress. This overview presents that rhizobacteria plays an important role in induced systemic resistance (ISR) to biotic stress or induced systemic tolerance (IST) to abiotic stress condition; bacterial determinants related to ISR and/or IST. In addition, we describe effects of rhizobacteria on defense/tolerance related signal pathway in plants. We also review recent information including plant resistance or tolerance against multiple stresses ($biotic{\times}abiotic$). We desire that this review contribute to expand understanding and knowledge on the microbial application in a constantly varying agroecosystem, and suggest beneficial microbes as one of alternative environment-friendly application to alleviate multiple stresses.

복합기능성 Bacillus sp. GH1-13 균주의 특징

김상윤(Sang Yoon Kim),상미경(Mee Kyung Sang),원항연(Hang-Yeon Weon),전영아(Young-Ah Jeon),류재환(Jae Hwan Ryoo),송재경(Jaekyeong Song) 한국농약과학회 2016 농약과학회지 Vol.20 No.3

토마토 뿌리에서 분리한 식물생육촉진 세균 Bacillus velezensis T20E-257균주의 유전체 염기서열

이신애,김상윤,상미경,송재경,원항연,Lee, Shin Ae,Kim, Sang Yoon,Sang, Mee Kyung,Song, Jaekyeong,Weon, Hang-Yeon 한국미생물학회 2017 미생물학회지 Vol.53 No.4

Bacillus velezensis T20E-257 was isolated from the root tissue of a tomato plant and exhibited plant growth-promoting activity. Here we present the complete genome of strain T20E-257. The genome contains 3,900,066 base pairs with a G + C content of 46.7% in 2 contigs. The genome includes 3,708 coding sequences, 27 rRNAs, and 86 tRNAs. We found gene clusters encoding secondary metabolites with an antimicrobial activity and genes related to the production of indole-3-acetic acid and 2,3-butanediol, which play a role in plant growth and health. 토마토 뿌리에서 분리한 Bacillus velezensis T20E-257 균주는 식물촉진효과가 있었고, 본 연구에서 T20E-257 균주의 유전체 서열을 해독하였다. 유전체 초안에서 포함된 2개 contig는 총 염기서열이 3,900,066 bp고, G + C content가 46.7%이었다. 유전체에서 단백질 유전자 3,708개, rRNA 유전자 27개, tRNA 유전자 86개를 확인하였다. 항균활성을 가지는 2차 대사산물 생합성 관련 유전자군과 식물생육촉진에 관여하는 IAA와 2,3-butadiol 생합성 관련 유전자를 T20E- 257 균주 유전체에서 확인하였다.

Trichoderma sp. GL02에 의한 고추 식물의 건조 스트레스 완화 효과

김상태 ( Kim Sang Tae ),유성제 ( Yoo Sung-je ),송재경 ( Song Jaekyeong ),원항연 ( Weon Hang-yeon ),상미경 ( Sang Mee Kyung ) 한국유기농업학회 2020 韓國有機農業學會誌 Vol.28 No.3

Drought stress is one of major environmental stresses in plants; this leads to reduce plant growth and crop yield. In this study, we selected fungal isolate for mitigating drought stress in pepper plants. To do this, 41 fungi were isolated from rhizosphere or bulk soils of various plants in Jeju, Gangneung, Hampyeong in Korea. Out of 41 isolates, we screened two isolates without phytotoxicity through seed germination of tomato, pepper, and cabbage treated with fungal spores; through following plant assay, we selected GL02 as a candidate for alleviating drought stress in pepper plants. As a result of greenhouse test of pepper plants in drought condition, the stomatal conductance on leaves of pepper plants treated with GL02 was increased, whereras the malondialdehyde (MDA) and electrolyte leakage were decreased compared to that in control plants. When stressed plants were rewatered, stomatal conductance of the plants treated with GL02 was increased; the electrolyte leakage was decreased. Based on internal transcribed spacer (ITS) sequencing analysis, isolate GL02 was belonging to genus Trichoderma. Taken together, drought stress in pepper plants treated with GL02 was alleviated, when it was rewatered after drought-stressed, the plants could be recovered effectively. Therefore, Trichoderma sp. GL02 could be used as a bio-fertilizer to alleviate drought stress in pepper plants.

Complete genome sequence of Chryseobacterium sp. T16E-39, a plant growth-promoting and biocontrol bacterium, isolated from tomato (Solanum lycopersicum L.) root

이신애,김상윤,상미경,송재경,원항연,Lee, Shin Ae,Kim, Sang Yoon,Sang, Mee Kyung,Song, Jaekyeong,Weon, Hang-Yeon The Microbiological Society of Korea 2017 미생물학회지 Vol.53 No.4

토마토 뿌리에서 분리한 Chryseobacterium sp. T16E-39 균주는 식물생육촉진과 역병, 시들음병에 대한 억제효과가 있었다. 이 균주의 유전체 염기서열은 4,873,888 염기쌍이었으며 G + C 함량은 35.22%이었다. 이 유전체는 4,289개 단백질 유전자, 15개 rRNA 유전자, 71개 tRNA 유전자를 포함하였다. T16E-39 균주의 유전체에서 인산가용화, 식물호르몬 조절, 항산화 활성, 키틴 분해, 제9형 분비시스템에 관여하는 유전자를 확인하였으며, 이들 유전자는 식물의 생육을 촉진하고 병발생을 억제하는 기작과 관련되어 있을 것으로 판단된다. Chryseobacterium sp. strain T16E-39, isolated from roots of a tomato plant, promotes plant growth and suppresses phytophthora blight and bacterial wilt diseases. The complete genome of strain T16E-39 consists of a circular chromosome with 4,872,888 base pairs with a G + C content of 35.22%. The genome includes 4,289 coding sequences, 15 rRNAs, and 71 tRNAs. We detected genes involved in phosphate solubilization, phytohormone regulation, antioxidant activity, chitin degradation, and the type IX secretion system (T9SS) that may be related to growth promotion and disease suppression in plants.

근권세균 Bacillus sp. CT16과 Neobacillus sp. JC05의 배양액 추출물에 의한 뿌리혹 선충의 알 부화 억제 효과

장화진(Hwajin Jang),김상태(Sang Tae Kim),상미경(Mee Kyung Sang) 한국식물병리학회 2021 식물병연구 Vol.27 No.2

식물 근권에서 180 균주를 분리하여 뿌리혹 선충(Meloido- gyne incognita)의 알 부화율 억제 효과가 있는 34개 균주를 일 차적으로 선발하였고, 이들 중 알 부화율과 유충 억제에 모두 효과가 있는 세균 JC05와 CT16을 선발하였다. 두 균주는 각각 Bacillus sp. CT16과 Neobacillus sp. JC05로 동정하였으며, 두 균 주의 배양액을 용매로 추출 및 농축한 후 선충의 알 부화율을 평가한 결과, JC05의 n-butanol 추출물은 25 μg/ml이상의 농도 에서 알의 부화를 효과적으로 억제했으며, CT16의 n-hexane과 n-butanol 추출물에서는 각각 25 μg/ml, 10 μg/ml 이상의 농도 에서 알 부화를 효과적으로 억제하였다. 오이 유묘 식물에 검정 한 결과, 100 μg/ml의 CT16와 JC05의 n-butanol 추출물을 오 이 유묘에 처리했을 때, 뿌리에 감염된 난낭의 수가 감소하였다. 이를 통해 JC05와 CT16가 생성하는 대사산물이 알 부화율 억제 활성을 보이는 것으로 생각되며 JC05와 CT16의 n-butanol 추출물이 선충방제로서 사용 가능성을 제시할 수 있다. 이후에 는 뿌리혹 선충 억제에 효과가 있는 추출물의 활성 물질을 동 정하고 오이 식물에서 작용 메커니즘 규명하기 위한 연구를 수 행하여 오이를 비롯한 박과류 뿌리혹 선충 억제를 위한 농업용 소재로 활용할 예정이다. Root-knot disease caused by Meloidogyne incognita is major soil pathogen and cause severe economic dam- ages to vegetable crops. In this study, we selected rhizobacteria for biocontrol of the root-knot nematode, M. incognita, and identified; performed bioassay of the bacterial extracts in cucumber seedlings. The crude extracts of strains CT16 and JC05 out of 180 strains inhibited egg hatching and increased juvenile mortality in vitro assay; based on 16S rRNA sequences analysis, the two strains were identified as Bacillus sp. CT16, and Neobacillus sp. JC05. After extracting the bacterial supernatants by using various organic solvents, n-butanol and n-hexane extracts of strain CT16 and n-butanol extract of strain JC05 showed inhibitory activity of egg hatching depending on concentrations. Subsequently, n-butanol extracts of two strains significantly sup- pressed formation of egg masses in cucumber seedling. Therefore, these results indicated that strains CT16 and JC05 could be used as potential biocontrol agents against M. incognita.

유용한 바실러스의 토양 접종에 따른 토착 세균 군집의 변화

김이슬 ( Yiseul Kim ),김상윤 ( Sang Yoon Kim ),안주희 ( Ju Hee An ),상미경 ( Mee Kyung Sang ),원항연 ( Hang-yeon Weon ),송재경 ( Jaekyeong Song ) 한국미생물생명공학회(구 한국산업미생물학회) 2018 한국미생물·생명공학회지 Vol.46 No.3

Beneficial microorganisms are widely used in the forestry, livestock, and, in particular, agricultural sectors to control soilborne diseases and promote plant growth. However, the industrial utilization of these microorganisms is very limited, mainly due to uncertainty concerning their ability to colonize and persist in soil. In this study, the survival of beneficial microorganisms in field soil microcosms was investigated for 13 days using quantitative PCR with B. subtilis group-specific primers. Bacterial community dynamics of the treated soils were analyzed using 16S ribosomal RNA (rRNA) gene amplicon sequencing on the Illumina MiSeq platform. The average 16S rRNA gene copy number per g dry soil of Bacillus spp. was 4.37 × 106 after treatment, which was 1,000 times higher than that of the control. The gene copy number was generally maintained for a week and was reduced thereafter, but remained 100 times higher than that of the control. Bacterial community analysis indicated that Acidobacteria (26.3 ± 0.9%), Proteobacteria (24.2 ± 0.5%), Chloroflexi (11.1 ± 0.4%), and Actinobacteria (9.7 ± 2.5%) were abundant phyla in both treated and non-treated soils. In the treated soils, the relative abundance of Actinobacteria was lower, whereas those of Bacteroidetes and Firmicutes were higher compared to the control. Differences in total relative abundances of operational taxonomic units belonging to several genera were observed between the treated and non-treated soils, suggesting that inoculation of soil with the Bacillus strains influenced the relative abundances of certain groups of bacteria and, therefore, the dynamics of resident bacterial communities. These changes in resident soil bacterial communities in response to inoculation of soil with beneficial Bacillus spp. provide important information for the use of beneficial microorganisms in soil for sustainable agriculture.