Effects of Short-term Acute Heat Stress on Physiological Responses and Heat Shock Proteins of Hanwoo Steer (Korean Cattle)

백열창,김민석,정진영,오영균,이성대,이유경,지상윤,최혁 사단법인 한국동물생명공학회 2019 한국동물생명공학회지 Vol.34 No.3

This study was performed to evaluate the effect of heat stress on the status of physiological responses, blood parameter, serum T3 and cortisol, and heat shock proteins (HSP 27, 70, and 90) of Hanwoo cattle. Six Hanwoo steers (242.8 ± 7.2 kg of BW) were housed in the climate-controlled respiration chambers. The experiment consisted of 7 days (control; 0 day) at thermoneutral (air temperature (Ta) of 15oC and relative humidity (RH) of 60%; temperature-humidity index (THI) = 64), and by 3 and 6 days (treatment groups) at heat stress (Ta of 35oC and RH of 60%; THI = 87). Body temperature of each parts (frank, rump, perineum and foot) and rectal temperature elevated in heat stress groups (3 days and 6 days) than the control group (0 day). Respiration rates increased in 3 days and 6 days (88.5 ± 0.96 bpm and 86.3 ± 0.63 bpm, respectively) from 0 days (39.5 ± 0.65 bpm). Feed intake significantly decreased in heat stress groups (3 days and 6 days, 3.7 ± 0.14 kg and 4.0 ± 0.15 kg, respectively) than the control group (0 day, 5.0 ± 0.00 kg). In addition, final BW significantly decreased in heat stress groups (3 days and 6 days, 211.8 ± 4.75 kg and 215.5 ± 3.50 kg, respectively) than the control group (0 day, 240.0 ± 25.00 kg). However, heat stress has no significant effect on blood parameter, serum T3 and cortisol. Nevertheless, heat stress increased HSPs mRNA expression in liver tissue, and serum concentration of HSPs. Despite Hanwoo cattle may have high adaptive ability to heat stress, our results suggested that heat stress directly effect on body temperature and respiration rate as well as serum and tissue HSPs. Therefore, we are recommended that HSPs could be the most appropriate indicators of Hanwoo cattle response to heat stress.

39. 흑염소 반추위 유래 복합 섬유소 분해효소의 in vitro 건물 소화율에 관한 연구

백열창,이경태,김도형,오영균 한국초지조사료학회 2014 한국초지조사료학회 학술대회논문집 Vol.2014 No.10

45. 흑염소 반추위 유래 섬유소 분해효소의 in vitro 건물 소화율에 관한 연구

백열창,이경태,박윤정,이강연,김경훈 한국초지조사료학회 2013 한국초지조사료학회 학술대회논문집 Vol.2013 No.09

42. 볏짚, 티모시, IRG, 호밀의 사료가치 평가에 관한 연구

백열창,이슬,이현정,송재용,김민석,오영균 한국초지조사료학회 2018 한국초지조사료학회 학술대회논문집 Vol.2018 No.09

사료용 쌀의 영양가치 및 이용방안

백열창 한국초지조사료학회 2017 한국초지조사료학회 학술대회논문집 Vol.2017 No.09

신이론 신기술 - 한우 사료로서 농식품 부산물 활용 방안

백열창,Baek, Yeol-Chang 한국사료협회 2012 飼料 Vol.55 No.-

호남지역 논 재배에 적합한 곡실 사료용 밀 품종 선발 및 사료가치 평가

백열창(Youl-Chang Baek),최혁(Hyuck Choi),김민석(Minseok Kim),박종호(Jong-Ho Park) 한국산학기술학회 2020 한국산학기술학회논문지 Vol.21 No.3

본 연구는 호남지역 유휴 논토양에서 곡실 사료용 밀 생산에 적합한 품종 선발을 위해 국내산 총 8개(금강, 조경, 백중, 수안, 청우, 고소, 호중, 조중)의 품종을 선택하여 전북 완주군에서 2015년부터 2016년까지 파종 및 수확하였으며, 사료가치 평가를 위해 일반성분 및 반추위 소화율 분석을 실시하였다. 시험품종 중 출수기는 “조중”이 4월 15일로 가장 빨랐으며, 성숙기 또한 5월 24일로 가장 빨랐다. “백중”의 출수기는 4월 18일로 “금강”과 같았고, 성숙기는 5월 29일로 하루 늦었지만 전 시험품종 간의 유의성은 없었다. 경수는 “고소”가 513개/m²로 가장 적었으며, “청우”가 974개/m²로 가장 많았다. “백중”의 경수는 901개/m²로 시험품종 중 경수가 많은 품종에 속하며, 수확량은 542kg/10a으로 가장 많았다(p <0.05). 일반성분 분석, 반추위내 건물 및 조단백질 소화율 평가에서 시험품종 간 유의적인 차이는 보이지 않았으며, 옥수수보다 높은 영양 가치와 소화율을 확인하였다. 따라서 상기 8개 밀 품종은 공히 수입곡물을 대체 할 수 있는 훌륭한 사료자원이며, 수확량이 높은 품종을 선택하여 곡실 사료로의 이용성을 확대하면, 논경지이용효율 증대와 더불어 축산농가 사료비 절감에 도움이 될 것으로 사료된다. The purpose of this study was to select the wheat varieties, among 8 domestic cultivates, that are suitable, for use as grain feed at paddy fields in the Honam region. The seeds of wheat were sown and harvested in Wanju County. Among the wheat varieties, "Jojoong" was the earliest for its heading date on April 15, and it showed the earliest day for the maturing date on May 24. The heading date of "Baegjong" was the same as that for "Keumkang" on April 18, and the maturing date was one day later than that for "Keumkang" on May 29. But there was no significant difference of heading and maturing dates among the 8 wheat varieties. The lowest number of spikes/m² was 513/m² of "Goso", and the highest number of spikes/m² was 974/m² of "Chongwoo". The spikes/m² and yield of "Baegjon" was 542kg/10a and it was the highest yielding variety among the 8 wheat varieties. The chemical compositions of DM (dry matter) and CP (crude protein) on rumen digestibility were not significantly different among the 8 wheat varieties. Furthermore, rumen digestibility of DM and CP, and the nutrients value were slightly higher than those of corn. The domestic 8 wheat varieties for grain feed, with their high nutrient qualities, could eventually replace imported grain feed and wheat grain feed. These 8 varieties are cost-effective feed and paddy field fodder for livestock farmers.

Evaluation of non-conventional feeds for ruminants using in situ nylon bag and the mobile bag technique

Youl-Chang Baek(백열창),Hyuck Choi(최혁) 한국산학기술학회 2017 한국산학기술학회논문지 Vol.18 No.7

본 연구는 반추동물 사료로서 한국에서 생산된 부존사료자원 15종의 화학적 조성, 소화율 그리고 에너지 가를 평가하기 위하여 실시하였다. 반추위 그리고 십이지장 캐뉼라가 장착된 거세한우 3두(평균체중 520 ± 20.20 kg)를 개별 계류식우사에 공시한 후 14일의 순치기간과 3일간의 시험기간을 두었다. 실험 방법으로는 화학조성 분석법, in situ 나일론백과 모바일백 방법을 이용하였다. 사료 내 화학적 조성을 분석한 결과, 엿밥, 깻묵, 장유박 및 두유박의 조단백질 함량은 30% 이상이었다. In situ 나일론백을 이용한 사료의 분해 특성을 분석한 결과, 비트펄프, 맥주박, 커피박, 엿밥, 수수겨, 임자박, 라면박 및 두유박의 반추위 미분해 단백질 함량은 50% 이상이었다. In situ 모바일백을 이용한 사료 내 가소화 영양소총량(TDN)의 분석은 비트펄프, 맥주박, 막걸리박, 수수겨, 깻묵, 라면박, 미강, 장유박, 비지, 두유박 및 밀기울이 50% 이상인 것으로 관찰되었다. 요약하자면, 상기 부존사료자원은 배합사료 또는 조사료를 대체할 수 있는 훌륭한 사료자원으로서의 높은 잠재적 가치를 가지고 있음을 시사하고 있다. 따라서 본 연구에서 얻은 부존사료자원의 화학적 조성, 소화율 및 에너지 가를 반추동물 섬유질배합사료 제조를 위한 기초 자료로 활용하고 이를 통해 사료효율 향상, 사료비용 절감 및 환경오염 감소에 기여하고자 한다. This study was conducted to evaluate the chemical composition, digestibility, and energy value of 15 non-conventional feeds produced in South Korea as ruminant feeds. Three Hanwoo steers (body weight, 520 ± 20.20 kg) fitted with a permanent rumen cannula and duodenal cannula were housed individually in tie-stall barns, followed by a 14-day adaptation period and 3-day experimental period.Chemical composition analysis, in situ nylon bag, and mobile bag technique were used as experiments. As a result of the chemical composition analysis offeeds, crude protein (CP) contentsofmalt meal, perilla meal, soy sauce cake, and soymilk residue were greater than 30%. As a result of the degradability characteristics analysis of feeds using an in situ nylon bag, rumen undegraded protein (RUP) contents of beet pulp, brewer"s grain, coffee meal, malt meal, milo bran, perilla meal, ramen residue, and soymilk residue were greater than 50%. Analysis of total digestible nutrient (TDN) values of feeds using an in situ mobile bag showed that TDN values of beet pulp, brewer"s grain, makgeolli residue, milo bran, perilla meal, ramen residue, rice bran, soy sauce cake, soybean curd cake, soymilk residue, and wheat bran weregreater than 50%. In summary, these non-conventional feeds have high potential value as good feed resources to replace formulated feeds or roughage. Therefore, the chemical composition, digestibility, and energy value of non-conventional feeds obtained from this study can beused as base data for the manufacture of ruminant total mixed ration (TMR) with improved feed efficiency, reduced feed costs, and reduction of environmental pollution.

Next Generation Sequencing을 통한 미생물 군집 분석의 축산분야 활용

김민석,백열창,오영균,Kim, Minseok,Baek, Youlchang,Oh, Young Kyoon 한국축산환경학회 2015 한국축산시설환경학회지 Vol.21 No.3

The objective of this study was to review application of next-generation sequencing (NGS) to investigate microbiome in the livestock sector. Since the 16S rRNA gene is used as a phylogenetic marker, unculturable members of microbiome in nature or managed environments have been investigated using the NGS technique based on 16S rRNA genes. However, few NGS studies have been conducted to investigate microbiome in the livestock sector. The 16S rRNA gene sequences obtained from NGS are classified to microbial taxa against the 16S rRNA gene reference database such as RDP, Greengenes and Silva databases. The sequences also are clustered into species-level OTUs at 97% sequence similarity. Microbiome similarity among treatment groups is visualized using principal coordinates analysis, while microbiome shared among treatment groups is visualized using a venn diagram. The use of the NGS technique will contribute to elucidating roles of microbiome in the livestock sector.

신규 조사료원 사료용 벼 사일리지의 사료가치 평가 및 급여 비율에 따른 반추위 발효성상 비교

박설화,백열창,이슬,김병현,류채화 한국초지조사료학회 2020 한국초지조사료학회지 Vol.40 No.4

This study was to evaluate the feed value of whole crop rice silage (WCRS) and to investigate a suitable ratio of the WCRS and concentrate by an analysis of rumen fermentation. A total of 6 treatments were used according to WCRS: concentrate ratio on in vitro rumen fermentation: T1 (100:0), T2 (60:40), T3 (40:60), T4 (20:80), T5 (10:90), and T6 (0:100). The ruminal pH, total gas emission, ammonia nitrogen, and volatile fatty acid (VFA) were determined as fermentation parameters. Total nutrients digestibility trial was conducted by 4 treatments according to WCRS: concentrate ratio at 40:60 (W40), 20:80 (W20), and 10:90 (W10), respectively. Feed value was analyzed according to AOAC (2019) and nutrient digestibility was calculated based on NRC (2001). The levels of crude protein (CP), crude fat, and neutral detergent fiber of the WCRS were 12.29%, 1.67%, and 59.79%, respectively. It was found to be 51.49% as a result of predicting the total digestible nutrient of WCRS using the NRC (2001) model. In vitro rumen fermentation, T4, T5, and T6 treatments showed a greater gas emission and total VFA concentration compared with other treatments (p<0.05). Acetate and acetate to propionate ratio of T4, T5, and T6 were significantly higher than other treatments (p<0.05). There was a significant difference in the level of propionate and butyrate according to the WCRS: concentrate ratio (p<0.05). The digestibility of dry matter and CP was significantly lower in W40 than in other treatments (p<0.05); however, there was no difference in W20 and W10. In conclusion, the 20:80 (WCRS: concentrate) is beneficial for stabilizing the rumen that does not inhibit rumen fermentation and nutrient digestion. This ratio might have a positive effect on the economics of farms as a valuable feed.