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임현진 ( Hyun Jin Lim ),백승옥 ( Seong Ok Back ),이범희 ( Bum Hee Lee ),유선우 ( Seon Woo You ) 대한임상검사과학회 2006 대한임상검사과학회지(KJCLS) Vol.38 No.2
One of the cardinal findings of the renal diseases is proteinuria, which appears in the early phase of kidney diseases and is very important in diagnosis, prognosis and decision making in the treatment process and results of the treatment. The study subjects were 126 patients who visited the nephrology department of Kangbuk Samsung Hospital. Serum was requested for urine protein electrophoresis. Total protein was measured with Bayer Advia 1650 (Biuret). Quantitation of each fraction was done by multiplying the percentage of each fraction by the total protein. Serum creatinine and BUN were also measured with Bayer Advia 1650 (Jaffe and Urease). Serum protein EP was done with REP(rapid electrophoresis) using Helena Kit reagents (REP Ultra SPE Kit, Ponceau S stain, Acetic acid, Methanol, EP Control). Concentrated urine was used for urine protein EP. The SPSS package was used for statistics analysis. Percentage and quantitation of the level of albumin in renal diseases were significantly lower than those in healthy controls. Total protein was correlated with albumin. In terms of proportion, α1-globulin, α2-globulin, β-globulin, and γ-globulin fractions were increased in the disease group. But, in the quantified level, α2-globulin was increased and β -globulin and γ-globulin were decreased. ESRD patients showed an increased secretion of high molecular proteins in urine protein EP. A decreased level in serum total protein correlated with the decreased level of serum albumin and the total amount of urine total protein. This study revealed the variety in the level of serum and urine proteins and their subgroups by EP.
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열 스트레스가 여성노인들의 근기능 및 근손상에 미치는 영향
박석(Park, Sok),이천호(Lee, Chone Ho),백승옥(Back, Seung Ok),신용업(Shin, Yong Up),김정석(Kim, Jung Suk),조영웅(Cho, Young Wung),이영준(Lee, Young Jun) 한국노년학회 2010 한국노년학 Vol.30 No.3
열 스트레스는 건강의 위험요인으로 장시간 노출시 열손상과 근기능 감소 등 신체 기능을 감소시키며 특히 생리적 기능이 감소되어 있는 노인에서 영향을 받기 쉽다. 본 연구는 여름철 폭염주의보 수준의 열 스트레스가 여성노인들의 근기능 및 근손상 지표에 미치는 영향을 살펴보고자 시도되었다. 65세 이상의 여성 자원자 총 8명을 대상으로 2주일 간격으로 상온(20°C) 그리고 폭염주의보 수준의 고온(33°C)에서 등속성 슬관절 근기능을 측정하였으며, 폭염주의보 수준의 열 스트레스가 근손상에 미치는 영향을 알아보기 위하여 근기능 측정 전과 후에 채혈을 실시하였다. 각속도 60°/sec 운동시, 고온(33°C) 환경에서 우측 굴곡근과 신전근에서 최대우력, 단위체중당 최대우력, 평균파워 그리고 단위체중당 평균파워에서 근기능이 감소하였다(p<0.05). 또한 각속도 180 °/sec 운동시, 고온(33°C) 환경에서 우측 슬관절 신전근 피로도가 증가하였으며(p<0.05), 굴곡근 총일량은 유의하게 증가하였다(p<0.05). 고온환경(33°C)에서 근손상 지표인 HSP70, CK 그리고 LDH는 상온(20°C)과 비교하여 유의하게 증가하였다(p<0.05). 결론적으로 열 스트레스가 여성노인들의 근기능을 감소시키고 근손상을 유발하는 것으로 나타났다. 따라서 여성노인들의 건강유지를 위하여 여름철 폭염환경에서 열 스트레스의 노출 및 신체활동을 최소화 시키는 방안이 효과적이라 사료된다. The purpose of this study was to examine the influence of extreme heat on muscle function and muscle injury marker in elderly women. The subjects of this study were eight post-menopausal elderly women without any metabolic disease. All eight subjects were asked to perform the knee joint isokinetic exercise using isokinetic equipment (cybex) in the laboratory and experimental temperature within laboratory was adjusted to two conditions: extreme heat temperature(33 ±0.5°C) and normal temperature(20±0.5°C) maintained in 50±3% humidity conditions. Each experimental exercise was monitored and analyzed the change of HSP70, LDH and CK. Muscular functions (peak torque, total work, percentage of peak torque body weight, fatigue index, average power and total work) were significant differences at exercise between temperatural conditions (p<.05). In extreme heat temperature, muscular injury markers (HSP70, LDH and CK) were increased, threfore resulted in significantly higher than normal temperature(p<.05). These results show that extreme heat temperature can decrease muscle function in elderly women.
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