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배수장,김광현,김병우,김영희,Bae, Soo-Jang,Kim, Kwang-Hyeon,Kim, Byung-Woo,Kim, Young-Hee 한국미생물 · 생명공학회 1995 한국미생물·생명공학회지 Vol.23 No.1
For using additives of foods, or cosmetics, a strain A80 producing blue pigment was isolated from soil. The strain A80 was identified as a strain of Azotobacter vinelandii based on morphological and physiological characteristics. The strain A80 was extracellulaly secreted the blue pigment on PYG agar plate, but not secreted it into PYG broth. And then, the strain A80 was extracellulaly secreted the blue pigment in PYG broth containing 2.0% chitin, while the strain A80 was not secreted the blue pigment in PYG broth containing 2.0% chitin and 1% NaCl simultaniously.
수용성 청색색소를 생산하는 Azotobacter vinelandii A80 균주의 분리 및 특성
배수장,김광현,김병우,김영희 한국산업미생물학회 1995 한국미생물·생명공학회지 Vol.23 No.1
식품이나 화장품의 첨가물로 사용하기 위해 청색색소를 분비하는 A8O 균주를 토양에서 분리선별하였다. 분리된 A8O 균주의 위상차 현미경에 의한 형태학적인 특성 및 생리학적인 특성을 조사한 결과 A8O균주는 Azotobacter vinelandii의 유연균으로 판정되었다. 또한 분리된 A8O 균주는 PYG 한천배지에서는 청색색소를 균체외로 분비하였으나, PYG 액체배지에서는 청색색소를 균체외로 분비하지 않았으며, PYG 액체배양에서도 2.0% chitin이 첨가되면 청색색소를 균체외로 분비하였으나 1.0% NaCl이 PYG 액체배지에 첨가되면 2.0% chitin이 첨가되어도 첨색색소는 전혀 생성되지 않았다. For using additives of foods, or cosmetics, a strain A8O producing blue pigment was isolated from soil. The strain A8O was identified as a strain of Azotobacter vinelandii based on morphological and physiological characteristics. The strain A8O was extracellulaly secreted the blue pigment on PYG agar plate, but not secreted it into PYG broth. And then, the strain A8O was extracellulaly secreted the blue pigment in PYG broth containing 2.O% chitin, while the strain A8O was not secreted the blue pigment in PYG broth containing 2.O% chitin and 1% NaCl simultaniously.
Immuno-Affinity Chromatography에 의한 B. thuringiensis H9B균주의 모기살충성 내독소 단백질의 정제
김광현,배수장,이광배 동의대학교 기초과학연구소 1998 基礎科學硏究論文集 Vol.8 No.1
For purification of a 70kDa toxic protein of mosquitocidal delta-endotoxin from B. thuringiensis strain H9B, immuno-affinity chromatography was performed. After separation of 70kDa toxic proteins from the delta-endotoxin of the strain H9B on SDS-PAGE, the 70kDa toxic protein was subcutaneously injected into rabbit for making a polyclonal antibody. A anti-70kDa toxic protein was purified by a column chromatography packed with proteinA-sepharose 4B gels. The 70kDa toxic protein from delta-endotoxin of the strain H9B was also purified by an immuno-affinity chromatography packed with CNBr-activated sepharose 4B gels conjugated anti-70kDa toxic protein after elution with 1/10M citric acid-1/5M Na₂HPO₄buffer(pH3.2) containing 0.5M NaCl. The 70kDa toxic protein was purified through only one step-separation system, was demonstrated by SDS-PAGE and immunoblot.
수용성 청색색소를 생산하는 Azotobacter vinelandii A80 균주의 분리 및 특성
김병우,배수장,김광현,김영희 동의대학교 기초과학연구소 1996 基礎科學硏究論文集 Vol.6 No.1
For using additives of foods, or cosmetics, a strain A80 producing blue pigment was isolated from soil. The strain A80 was identified as a Azolobacter vinelandii based on morphological and physiological characteristics. The strain A80 was extracellulaly secreted the blue pigment on PYG agar plate, but not secreted it into PYG broth. And then, the strain A80 was extracellulaly secreted the blue pigment in PYG broth containing 2.0% chitin, while the strain A80 was not secreted the blue pigment in PYG broth containing 2.0% chitin and 1% NaCl simultaniously.