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정화영 ( Hwa Young Jung ),김미리 ( Mi Ri Kim ),박영민 ( Young Min Park ),박철종 ( Chul Jong Park ),김진우 ( Jin Woom Kim ),김경문 ( Kyung Moon Kim ),이정덕 ( Jeong Deuk Lee ),강훈 ( Hoon Kang ),조백기 ( Baik Kee Cho ),박현정 ( Hy 대한피부과학회 2015 대한피부과학회지 Vol.53 No.1
Background: Rosacea is characterized by erythema of the central face that persists for several months or longer. Reports of the histological changes in rosacea are scarce, and few attempts have been made to correlate suchchanges with clinical findings and pathophysiology. Objective: This study aimed to elucidate the clinical manifestations of rosacea and investigate its histologicalfeatures. Methods: We performed a retrospective analysis of 278 patients with histologically confirmed rosacea who visitedthe Department of Dermatology at the Catholic Medical Center between January 2008 and May 2013. Clinicalsubtypes, disease severity, and precipitating factors were evaluated. In 115 randomly selected patients, histopathologicalfeatures were evaluated as well. Results: The ratio of males to females was 1:1.8. The age distribution showed a peak incidence in the fifthdecade. The most common subtype was papulopustular rosacea (52.9%) followed by erythematotelangiectatic rosacea(34.9%), ocular rosacea (4.0%), and phymatous rosacea (2.9%). Granulomatous rosacea accounted for 5.4% ofrosacea cases. Precipitating factors included hot weather (54.7%), stress (51.8%), sun exposure (37.4%), alcohol(37.4%), and hot baths (33.1%). Histological analysis of skin biopsies from 115 patients revealed solar elastosis in62 patients (53.9%) and telangiectasia in 85 patients (73.9%).Conclusion: In this study, Korean rosacea patients were predominantly female with a peak age in the fifth decadeand the majority suffered from the papulopustular and erythematotelangiectatic types of rosacea. Histologicalobservations pertaining to each rosacea type were also discussed. (Korean J Dermatol 2015;53(1):1∼9)
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체외수정 배양액에 대한 제대혈청 및 단백질 첨가의 난자성숙 및 난구 세포 분산에 미치는 영향
신현우,이여일,박현정 대한산부인과학회 1995 Obstetrics & Gynecology Science Vol.38 No.7
본병원 체외수정 시술후 임신된 배양액군과 임신에 실패한 배양액군에서 각각 Ham`s F-10 기본 배양액 및 소혈청단백 또는 태아제대혈청 첨가 배양액하에서 생쥐의 난자-난구 복합체 를 22시간 동안 배양하고 난자의 성숙도와 난구세포 분산정도를 비교분석한 결과 다음과 같은 성적을 얻었다. 1. 난자성숙의 진행정도는 Ham`s F-10 기본배양액과 소혈청단백 및 태아제대혈청첨가 배양 액 사이에 유의한 차이가 없어 두가지 첨가물 모두 난자 성숙에는 별로 영향을 주지않는 것으로 보인다. 2. 난구세포 분산에 있어서는 태아제대혈청 첨가배양액 만이 저명한 발현을 보여 이 혈청내 난구세포 분산 자극물질의 존재를 시사하였다. 3. 난자성숙과 난구세포 분산 공히 기본 배양액과 첨가 배양액에서 임신군과 비임신군 간에 유의한 차이가 없어 체외수정 성공여부와는 무관한 결과를 나타냈었으나, 생쥐 난자-난구 복합체 배양은 종전의 배아 배양 정도관리시 파악할 수 없었던 수정전 및 수정시기의 배양 조건의 질적평가에 새로운 시야를 제공하였다. OBJECTIVE : To investigate of effect of human cord serum(HCS) and bovine serum albumin(BSA) supplementation on oocyte maturation and cumulus cell expansion in vitro culture media. DESIGN : Random sampling of 6 conceptional media from 7 patients and 8 nonconceptional media from 14 patients resulted from our in vitro fertilization and embryo transfer(IVF-ET) program. Each medium was divided to three groups : Ham`s F-10(unsupplemented), Ham`s F-10 supplemented with 0.4% BSA and Ham`s F-10 supplemented with 10% HCS. Setting, Patients : In vitro fertilization (IVF) program and patients at the IVF clinic of Chonman University Hospital Kwangju. Korea. Interventions : oocyte cumulus cell(OCC) complexes were collected from removed ovaries of pregnant mare serum gonadotropin(PMSG) primed mice, and cultured in three different media. Main outcome Measures : The oocyte maturities were measured by the presence of germinal vesicle breakdown and polar body, and the cumulus cell expansions were analyzed by the degree of cellular dispersion. Results : Oocyte maturity was not significantly different among the three types of media. cumulus cell expansion was significantly increased only by HCS-supplemented media. Comparison of oocyte maturity and cumulus cell expansion among groups showed no significant differences between conceptional and nonconceptional media. Conclusions : The supplementions with HCS or BSA are not able to augment mouse oocyte maturation. The great cumulus expansion in HCS supplemented media suggest the presence of stimulatory in OCC complex. The OCC complex culture provides new insight into qualitative information on prefertilization and perifertilization events.
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