Comparison of Results between Cytogenetic Technique and Molecular Genetic Technique in Colorectal Carcinoma Patients

박철인,이재식,Park, Cheolin,Lee, Jae Sik Korean Society for Clinical Laboratory Science 2017 대한임상검사과학회지(KJCLS) Vol.49 No.3

Globally, 1.3 million people develop colon cancer every year, and 600,000 people die each year it. In Korea, colorectal carcinoma was associated with the highest death rate, accounting for 8,380 people, among solid cancers in 2015. Among the various methods for the diagnosis and study of colorectal carcinoma, the results obtained by cytogenetic and molecular genetic methods were compared. Detection rate was 47% in 18q, 40% in 17p, 27% in 22q, and 17% in 10q via CGH; detection rate was 57% in D18S59, 50% in D18S68, 50% in TP53CA, 47% in D18S6940% in D22S274, 37% in D22S283, 27% in D10S187, and 23% in D10S541 with LOH. Microsatellite marker matching rates were 100% in D22S274, 100% in D22S283, 100% in D10S186, 100% in D10S187, 100% in D10S541, 93% in D18S69, 93% in D18S68, 92% in TP53CA, and 89% in D18S59. The agreement rate between the two methods was 94.4% based on positive results using CGH. Based on the advantages of CGH, which was the ability to obtain information regarding the entire tumor genome at once, this experiment could identify the region with significant deletion using CGH and the more limited region LOH, with a completely different approach. LOH in the recurrent high-risk group, 18q21, was helpful in the selection of treatment modalities and in prognostic estimation as well as making the most appropriate decision for treatment. Therefore, it is suggested that LOH with surgical site tissues could be one of the treatment methods for recurrent high-risk group among patients with colorectal carcinoma.

교육환경 변화에 따른 전문대학 수업 연한 다양화에 대한 인식조사

박철인(Cheolin Park),박수진(Su-Jin Park),권순무(Soon-Mu Kwon),김원기(Won-Gi Kim),장기환(Ki-whan Chang) 한국산학기술학회 2018 한국산학기술학회논문지 Vol.19 No.4

본 전문대학은 고등교육기관으로 우리나라 산업발전에 필요한 인적자원을 제공함으로서 국가경제 발전의 원동력으로서 큰 역할을 하였으나 최근 학령인구 감소로 인한 전문대학의 어려움이 예상되고 있다. 따라서 급변하는 환경변화 속에서 전문대학이 고등직업교육을 대표하는 기관으로서의 역할과 상호협력과 경쟁을 통한 생존전략이 필요한 시점이다. 이에 본 연구에서는 전국 보건대학의 보건계열 중심으로 현황을 분석하고, 전문대학에 대한 정책변화와 대학에 제시하고 있는 방향성을 조사하여 전문대학 수업연한 및 학위과정 다양화 추진을 위한 구체적인 방안마련의 근거자료로 활용하고자 실시되었다. 본 연구는 현재 전국 보건대학 보건계열학과에 재직 중인 교수와 산업체 종사자 636명을 2017년 5월 1일∼5월 30일까지 설문지를 이용하여 조사하였다. 연구결과, 3년제 보건계열학과의 4년제 전환에 대해 찬성한다는 의견이 70.7%였다. 이유로는 충분한 수업연한으로 현장실습 및 인성교육을 강화할 수 있고, 동등한 학력을 가질 수 있기 때문인 것으로 나타났다. 4년제 전환 시 가장 기대되는 효과는 의료기사의 사회적 위상 향상과 대학의 교육환경개선이었으며, 4년제 전환을 위해 대학, 협회, 교육부가 주도적인 역할을 수행해야 한다고 하였다. 본 연구의 결과를 토대로 다가오는 4차 산업혁명시대에 요구되는 전문보건의료인 양성을 위한 더 체계적이고 고도화된 직업교육체제가 필요한 상황에서 전문대학의 수업연한 조정을 통한 학위과정의 다양화를 자유롭게 열어놓아 일반대학 졸업자들과의 경쟁력을 갖추어 선진 보건의료인 양성에 적극적으로 대비를 해야 할 것으로 사료된다. Junior colleges are higher education institutions that have played a major role in the economic development of Korea by providing the necessary human resources for its industrial development. Recently, however, they have experienced difficulties due to the reduction in the number of students. Therefore, it is time for junior colleges as a representative higher vocational education institution to change their role in this rapidly changing environment, and adopt a survival strategy through mutual cooperation and competition. The purpose of this study was to analyze the current state of the national health universities, to investigate the policy changes adopted by colleges and universities, and to utilize the results as data. This study analyzed the current status of health science colleges nationwide and investigated the policy changes as well as the directions presented to the colleges, in order to use the results as the basic data to promote the diversification of the class periods and degree programs. This study surveyed 636 professors from health sciences departments and industry workers from May 1 to May 30, 2017. 70.7% of the respondents supported the transition of the existing three-year systems of the health science departments to four-year systems. The reason for this is that it is possible to strengthen the field practice and personality education of the students by having a sufficient number of class periods, and to provide them with an equal educational background. The most anticipated effect of the transition to a four-year system is to improve the social status of medical personnel and to improve the educational environment of the colleges/universities. Moreover, the universities, associations of medical personnel and Ministry of Education are expected to play a leading role in the transition to the four-year system. Based on the results of this study, it was concluded that a more systematic and advanced vocational education system for the training of professional healthcare workers is needed in the upcoming fourth Industrial Revolution era. Also, this transition is expected to actively foster the education of advanced health care workers thanks to the diversification of the degree programs through the adjustment of the class periods which can be completed by general university (4-year) graduates.

Management of Diabetes from HbA1c Test

이재식,박철인,김철회 대한임상검사과학회 2010 대한임상검사과학회지(KJCLS) Vol.42 No.3

HbA1c test measures the amount of glycated hemoglobin in blood. HbA1c shows the average of blood glucose levels for the past three months, this is a better indicator of how overall diabetes is doing. HbA1c gives a much better idea of how the body is breaking down the glucose. Therefore, this HbA1c is very important tool for maintaining normal glucose levels for pre-and diabetic patients. Total 408 participants were tested HbA1c voluntarily from Chosunilbo Health Expo (8th~11th, July 2010). Through this small-scaled direct HbA1c, about 54.7% (207 out of 408) was shown glucose tolerance and diabetes. However, 61 from 157 participants who were shown under 6.9% HbA1c (normal and pre-diabetic stage) are taking only antidiabetic drugs to maintain a normal blood glucose. Regular HbA1c test can bring an important management and awareness about controlling blood sugar level and prevention of diabetic complications. .

배후발생동안 누에나방 중추신경계에서 Leucokinin I 면역반응성의 변화

강종석,김나영,박중철,김기성,박철인,이봉희 한국곤충학회 1998 Entomological Research Vol.28 No.2

곤충의 신경펩티드는 곤충의 전 발생과정에서 뉴런이 합성하여 분비하는 펩티드이다. 본 논문에서는 면역 세포화학법을 이용하여 누에나방(Bombyx mori)의 5령유충, 용 및 성충중추신경계에서 leucokinin I 면역반응성 뉴런의 수 및 그 세포에서 뻗어나오는 축색돌기의 변화를 관찰하였다. 5령 유충기에서는 뇌의 양쪽 상단에 대칭적으로 5쌍의 LK I-IR neuron과 뇌 중앙에 1쌍이 위치해 총 12개의 LK I-IR neuron이 분포하였다. 용기단계와 성충기에서는 모두 뇌 중앙부의 양쪽에 모두 3쌍의 LK I-IR neuron이 관찰되었다. 식도하신경절과, 흉부신경절에서는 면역반응성이 발견되지 않았다. 복부신경절에서는 5령유충, 용, 성충 모두에서 3번째 복부신경절, 4번째 복부신경절, 5번째 복부신경절, 6번째 복부신경절, 7/8번째 복부신경절 모두에서 각 신경절하단의 양쪽 가장자리에서 1쌍의 LK I-IR neuron이 분포하는 것이 관찰되었다. LK I- IR neuron에서 뻗은 축색돌기들은 nerve II (N II)로 뻗어나갔다. Antisera against leucokinin I, isolated from head extracts of the cockroach were used to study peptidergic neurons and their innervation patterns in the central nervous system (CNS) of Bombyx mori during postembryonic development. In case of brain, about 12 leucokinin I-immunoreactive (LK I- IR) neurons are found in the protocerebrum of larval brain, but their number decreased to about six LK I- IR neurons in the pupal and adult stages, respectively. In ventral nerve cord, there was no immunoreactivity in the subesophageal and thoracic ganglia, whereas abdominal ganglia 2 to 7 were shown to contain LK I-IR neurons in larval, pupal and adult stages, respectively. Especially, axons of these LK I-IR neurons projected from brain into NII. Therefore, the distribution of immunoreactivity indicates that LK I has an important actions as neurotransmitter or neuro-hormones in different neuromeres of the brain and ventral nerve cord during postembryonic development.

In Vitro Evaluation of Antibacterial Activities and Anti-inflammatory Effects of Bifidobacterium spp. Addressing Acne Vulgaris

이도경,Nam Joo Ha,Mi-Jin Kim,Ji-Wook Ham,Hyang-Mi An,Min-Kyung Cha,Si-Won Lee,박철인,신선희,Kang-Oh Lee,김경제 대한약학회 2012 Archives of Pharmacal Research Vol.35 No.6

The objective of this study was to evaluate the antibacterial and anti-inflammatory effect of Bifidobacterium spp. In the first part of the study, the antibacterial activities of live and sonicated cells, from a total of 23 Bifidobacterium species, on the growth of 5 different strain of Staphylococcus aureus. Six strains, of sonicated Bifidobacterium, exhibited antibacterial activity against staphylococci samples, and seven Bifidobacterium strains exhibited antibacterial activity on the growth of S. aureus S.P.-N2. In the second part of the study, we tested the antimicrobial activity, of Bifidobacterium against Propionibacterium acne KCTC3320, using the co-culture method. The loss of P. acnes viability, caused by B. adolescentis SPM0308 and B. longum SPM1207, was 84% and 75%, respectively (*p < 0.05). In the third part of the study, the anti-inflammatory activity of B. adolescentis SPM0308 and B. longum SPM1207 were assessed; nitric oxide (NO), and tumor necrosis factor-α (TNF-α), production were tested using the murine macrophage RAW 264.7 cell line. Treatment of RAW 264.7 cells, with Bifidobacterium, decreased production of NO and TNF-α rather than LPS (100 ng/mL) treatment. The results suggest that B. adolescentis SPM0308 could be used as an effective control for P. acnes KCTC3320, and S. aureus, and reduce the risk of acne vulgaris development. We suggest that B. adolescentis SPM0308 may be a useful probiotic microorganism, for prevention of acne vulgaris, without adverse effects.