저아밀로스 특성 및 쌀가루 적합 벼 품종 육성

박재령,김은경,장윤희,김경민 한국생명과학회 2022 생명과학회지 Vol.32 No.9

우리나라의 쌀 소비량은 지속적으로 감소하고 있으며, 쌀 재고량을 감소시키는 방안이 끊임없이 제안되고있다. 쌀 소비량을 늘려 재고되는 양을 감소시키기 위해서는 단순히 밥으로 이용되는 것 이외에 새로운 쌀 가공 산업이 발전되어야 한다. 또한 최근 우리나라 국민들의 식생활이 다양해지고 있으며, 빵과 면의 소비가 증가하고 있다. 따라서 쌀의 용도를 다양화하기 위해 쌀가루 전용 품종을 육성하였다. 쌀 가루 가공에 적합한 계통을 육성하기 위해 일품을 조직배양 하여 돌연변이 집단을 구축하였다. 돌연변이 집단은 포장에서 주요 농업 형질에 대해서 조사 되었고, 수확 후에는 낟알 특성과 배유의 특성이 조사 되었다. 이들 계통 중 모품종인 일품과 농업형질이 비슷하고 품질이 우수한 29111 계통이 선발 되었다. 또한 식미를 결정하는 이화학적 특성이 일품과 유사하거나 향상 되었다. 하지만 29111는 일품과 달리 전분구조는 균일하지 않고 대형 전분립과 수형전분립이 골고루 분포되어 있어 분상질 배유의 특성을 가진다. 따라서 29111은 가공에 적합하면서 농업형질과 식미가 우수한 계통으로 육성되었다. 쌀 용도를 다양화하여 부가가치를 향상 시킬 수 있다. 또한 식미를 향상 시켰으며 생산자와 소비자 모두가 만족 할 수 있는 반찹쌀용과 분상질 미립 특성을 가진 품종 육성 방법을 제시하였다.

호남평야지 재배시기별 찰벼 품종의 수량과 호화점도 및 식감 관련 특성 분석

박재령,서정환,이창민,박송희,진민아,정오영,백만기,박현수 한국육종학회 2023 한국육종학회지 Vol.55 No.1

The cultivated area of glutinous rice in the Homan Plain of Korea is increasing to diversify the cropping system. Depending onthe time of transplanting, glutinous rice can be divided into early, ordinary, and late cultivation. Eight glutinous rice cultivars (Baegseolchal,Baekogchal, Boramchal, Boseogchal, Dongjinchal, JJ644wx, Nunbora, and Sinseonchal) were used to evaluate yield, pasting properties, andtexture according to cultivation time and to analyze the genotype-environment relationship. There were nine yield-related, six pasting-related,and four texture-related traits studied. Heading date and culm length were shortened as cultivation time increased, while panicle length, numberof spikelets, and 1,000-grain weight increased. Furthermore, late cultivation time also reduced pasting properties, peak viscosity, trough viscosity,and final viscosity. Hardness and adhesiveness among the texture properties increased with cultivation time, but stickiness decreased. Thevariation in 1,000-grain weight is mostly dominated by genotype, whereas heading date and yield are heavily influenced by environmentalfactors. All pasting property characteristics varied with environment changes, and genotype variation was dominant for all texture properties. Boramchal yielded the most in early and ordinary cultivation and Sinseonchal, together with Boramchal, yielded the most in late cultivation. Cultivars with excellent grain quality were Sinseonchal in early cultivation and Baegseolchal and Baekogchal in ordinary and late cultivation. A comparative analysis of characteristics based on cultivation time can assist in the selection of glutinous rice suitable for the Honam Plainand in the breeding of cultivars with improved yield and excellent grain quality.

습식 나노화 공정기술 적용 식물 영양제를 살포한 사과의 칼슘과 마그네슘 이동 및 흡수율 비교

박재령(Jae-Ryoung Park),김은경(Eun-Gyeong Kim),이승현(Seung Hyun Lee),정일경(Il Kyung Chung),김경민(Kyung-Min Kim) 한국생명과학회 2019 생명과학회지 Vol.29 No.7

본 연구에서는 습식 나노화 공정 기술을 적용하여 제조한 식물영양제를 이용하여 사과 잎에 흡수되는 칼슘과 마그네슘의 이동 경로 및 흡수율을 비교 분석하였다. 습식 나노화 공정을 통해 불용성 칼슘과 마그네슘을 나노화 처리한 식물영양제의 성분 함량 및 이동경로를 확인하기 위하여 식물 영양제 살포 후 2주, 4주 및 8주 경과 후 잎자루, 잎몸, 옆면으로 구분하여 SEM, EDS 촬영을 통해 분석하였다. 잎자루는 식물 영양제 살포 후 4주차부터 증가하여 8주차에서는 1,115%까지 증가했으며, 사과 잎몸의 경우 2주차의 미살포구에 비해 살포 후 칼슘과 마그네슘의 함량이 감소하였으나, 4주 이후부터는 증가하였으며, 4주차의 뒷면 증가율이 539%로 가장 높았다. 또한, 잎몸의 옆면은 미살포구에 비해 살포구 모두 칼슘과 마그네슘 함량이 증가하였는데 특히, 4주차에 673% 증가하여 가장 높은 증가율을 보였다. 시간이 경과함에 따라 모든 살포구가 미살포구에 비해 칼슘, 마그네슘 함량이 점점 증가하여 나노화 식물영양제의 불용성 칼슘과 마그네슘 성분이 사과의 잎자루에서 잎몸까지 이동한 것으로 판단된다. 본 연구 결과를 보았을 때 습식 나노 공정 기술을 통해 제조된 식물영양제를 활용하면 사과 이외의 다른 작물 에서도 칼슘과 마그네슘 흡수 효과를 얻을 수 있을 것으로 판단하였다. 또한, 식물영양제 이외에도 천연물이나 바이오산업에도 나노 공정 기술의 적용이 가능할 것으로 기대된다. In this study, the migration route and the absorption rate of calcium and magnesium in apple leaves were compared and analyzed using plant nutrients prepared by wet nano-grinding technology. The plant nutrients were sprayed onto the leaves to confirm the component content and the movement route of the nanoized calcium and magnesium. At 2, 4, and 8 weeks after the plant nutrient treatment, the apple leaves were divided into petiole, lamina, and side, and SEM and EDS were used to measure the calcium and magnesium contents. The calcium and magnesium contents of the petiole increased from the 4th week after plant nutrient application to 1,115% at the 8th week. The calcium and magnesium contents of the lamina decreased after spraying but increased after 4 weeks. The calcium and magnesium contents increased in the side of the leaves compared to the control, reaching 673% after 4 weeks. The calcium and magnesium contents increased with increasing duration in all plots when compared with the control unsprayed leaves, suggesting that the usually poorly soluble calcium and magnesium were transferred from the petioles to the lamina. The results of this study indicate that improved calcium and magnesium absorption could be obtained in crops other than apples using plant nutrients produced through wet nano-processing technology. This technology is also expected to be applicable to natural products and bioindustries.

단백질 함량 증가 고품질 특수미 ‘드리미5호’ 육성

박재령(Jae-Ryoung Park),김은경(Eun-Gyeong Kim),장윤희(Yoon-Hee Jang),이강섭(Gang-Seob Lee),김경민(Kyung-Min Kim) 한국육종학회 2022 한국육종학회지 Vol.54 No.4

Rice is a staple food in most countries, and thus, diversifying its value is necessary. Recently, the focus on good health has increased, and rice cultivars with improved properties should be bred based on consumer demands. At the Plant Molecular Breeding Laboratory of Kyungpook National University, “Hwayeong” was tissue-cultured to derive mutant populations. In the mutant lines, “P35” with high protein content and excellent agricultural traits, was backcrossed with “Ilmi” three times, and the final selected line was called “Drimi5ho.” The heading date of “Drimi5ho” was August 11 (107 d after sowing), which was 5 d later than that of “Ilmi” (August 5, 101 d after sowing). The culm length of “Drimi5ho” was 71 cm, the panicle length was 18 cm, the number of panicles per hill was 13, the number of spikelets per panicle was 113, the ratio of ripened grain was 90.5%, and the 1,000-grain weight was 22.6 g. When “Drimi5ho” was planted ordinary plantation, the yield in the southern mountainous area was 526 kg/10a. Lodging was not observed when “Drimi5ho” was grown in the field with standard fertilization (N-P2O5-K2O = 9-4.5-5.7 kg/10a). This rice cultivar was resistant to leaf/neck blast, bacterial blight (K1, K2, and K3), rice stripe virus, rice black-streaked dwarf virus, and rice dwarf virus. The milled grain of “Drimi5ho” was clear and transparent without any chalkiness; moreover, the grain shape was round and short. Although it is a specialty rice with increased protein content, the taste of the rice and the grain quality were excellent (Registration No. 6829).

패키지 디자인 배색에 따른 단맛 연상 연구

박재령(JaeRyung Park),박영경(YungKyung Park) 한국색채학회 2016 한국색채학회 학술대회 Vol.2016 No.11

본 연구에서는 단맛 제품 패키지 디자인에 있어 배색이 단맛의 연상에 미치는 영향을 연구함으로서 단맛 제품의 패키지 디자인시 배색 적용에 관한 기본자료를 제공하고자 한다. 이를 위하여 KS 기본색 15색을 2색 배색한 샘플을 제작하여 이에 대한 패키지 디자인 배색의 단맛연상도를 알아보는 설문을 진행 하였다. 설문을 분석한 결과 Pk, Y 배색의 경우 단맛을 연상시켰다. Pk 배색의 경우 가장 강하게 단맛이 연상되었고, Wh 또는 난색계열인 R, Y, YR와의 배색 시 강한 단맛의 연상을 보였다. 반면 BG, PB, Gy, Bk의 배색 경우 쓴맛이 연상되었다. Bk 경우 가장 강하게 쓴맛이 연상되었으며, Gy 또는 한색 계열인 BG, PB와 배색시 강한 쓴맛의 연상을 보였다. RP, YR, Wh, R, P, YG, Br, B, G 배색의 경우 단맛과 쓴맛을 제외한 그 외의 맛 혹은 맛이 느껴지지 않는다고 연상하였다. 실험분석 결과를 통해 2색배색시 특정 단색이 갖고 있는 단맛 연상도가 패키지 디자인 배색의 단맛 연상도에 영향을 준다는 것을 알 수 있었으며, 단맛이 연상되는 단색 색상들의 배색시 단맛연상도가 높다는 것을 알수 있었다. 그러나 가장 강한 단맛 연상이 일어난 패키지 배색은 Pk와 Wh의 배색이었다. 본 연구를 통해 단맛 제품의 패키지 디자인시 배색에 따른 단맛연상도를 고려하여 제품의 효과적인 이미지 전달에 기여할수 있기를 기대한다.

미국 벼 품종의 입형과 호화점도 및 식감 관련 형질 특성 분석

박재령(Jae-Ryoung Park),이창민(Chang-Min Lee),백만기(Man-Kee Baek),안주현(Ju Hyeon An),서정환(Jeonghwan Seo),홍하철(Ha-Cheol Hong),정오영(O-Young Jeong),박현수(Hyun-Su Park) 한국육종학회 2022 한국육종학회지 Vol.54 No.2

Temperate japonica is used as the parent for crossing in the breeding of new rice cultivars in Korea, and the grain shape of the developed lines or cultivars is a semi-round and medium-short grain length. To overcome the current low quantity of japonica cultivar germplasm and deterioration in quality caused by climate change, it is necessary to diversify the grain characteristics of japonica. Most of the United States (US) cultivars are classified as medium, long, extra-long, and half spindle- and spindle-shaped grains. The qSW5 allelotype, a major gene involved in grain width, is found in all US cultivars. In addition, the US cultivar ‘Saber’ has both qgl3 and GS3_B alleles, which have low allele frequencies among Korean rice cultivars, and ‘A020’ had gw8, an allele with a reduced grain width. The pasting properties and texture of medium-grain length and half-spindle-shaped cultivars were relatively similar to those of the Korean rice cultivar, but different from those of long-grain and long spindle-shaped cultivars. Therefore, grain shape is associated with pasting properties and texture traits. Among the rice cultivars in the US, tropical japonica belongs to the same japonica as the Korean rice cultivar; however, its grain shape is similar to that of indica. As grain shape is also related to grain quality, US cultivars can be used as a useful genetic resource for diversifying the grain characteristics of Korean japonica cultivars and breeding new cultivars.

거대배 복합 내병충성 벼 품종 ‘드리미4호’ 육성

김은경,박재령,장윤희,손재근,이강섭,김경민 한국육종학회 2023 한국육종학회지 Vol.55 No.1

Rice is an important food crop that feeds most of the world's population. However, due to the increased frequency of pest outbreaks,it is necessary to cultivate complex pest-resistant rice cultivars. A mutant population was derived by culturing tissue of ‘Hwayeong’ at thePlant Molecular Breeding laboratory of Kyungpook National University in the Republic of Korea. The cultivar ‘Drimi4ho’, a line that wasresistant to complex disease, was finally selected by backcrossing with ‘Junam’. The heading date of ‘Drimi4ho’ is August 11 (107 daysafter sowing). Drimi4ho’ has culm length of 69 cm, panicle length of 21 cm, 16 panicles per hill, 115 spikelets per panicle, a ratio of ripenedgrain of 92.7%, and a 1,000-grain weight of 24.3 g. No lodging occurred when ‘Drimi4ho’ was grown in the field by standard fertilization. It is also resistant to leaf/neck blast disease, bacterial blight (K1, K2, K3), rice dwarf virus, rice black-streaked dwarf virus, rice stripe virus,BPH, and SBPH. ‘Drimi4ho’ is a round and short-type grain, and is very clear and transparent without chalkiness. In addition, it has excellentphysicochemical properties that make up food, so its taste is good. When ‘Drimi4ho’ was planted under ordinary conditions in the southernmountainous area of Korea, the yield was 5.53 MT/ha (Registration No. 6124).

고품질 복합내병충성 벼 품종 ‘드리미2호’ 육성

김은경,박재령,장윤희,손재근,이강섭,김경민 한국육종학회 2022 한국육종학회지 Vol.54 No.3

Rice is an important crop used as a staple food in most of the world; however the occurrence of pests is increasing due to climatechange. In particular, the brown planthopper (BPH, Nilaparvata lugens stal) is a key cause of rice yield loss. In response to the era of unpredictableclimate change in the future, the Plant Molecular Breeding laboratory of the Department of Applied Biosciences, Graduate School, KyungpookNational University, Daegu 41566, Korea bred the ‘Drimi2ho’ strain, which is resistance to BPH or various pests and of excellent eatingquality. In order to cultivate ‘Drimi2ho’, ‘Samgang’ and ‘Nagdong’ were crossed and the derived seeds were backcrossed with ‘Junam’, andthe pedigree breeding method was applied. During the application of the pedigree method, agricultural characteristics which are investigatedin the field were found to be excellent, and the best line for various disease resistance, grain quality, and yield was finally selected as ‘Drimi2ho’. ‘Drimi2ho’ has strong resistance to BPH and its range of resistance to major pests and viral diseases such as blast and bacterial blight onrice is quite wide. In the field, the main agricultural characteristics of ‘Drimi2ho’ were similar to or improved from those of ‘Junam’. ‘Drimi2ho’is a cultivar with enhanced disease and pest resistance and a wide spectrum while maintaining the good eating quality. ‘Drimi2ho’ yielded584 kg/10a in ordinary cultivation under normal level of fertilization in south mid-mountainous areas of Korea. Drimi2ho’ can thus be astandard cultivar in Korea due to its varied resistance to multiple diseases and excellent grain quality. (Registration No. 4686).

자포니카 벼멸구 저항성 벼 품종 ‘드리미1호’ 육성

장윤희,박재령,김은경,손재근,이강섭,김경민 한국육종학회 2022 한국육종학회지 Vol.54 No.3

Brown planthopper (BPH, Nilaparvata lugens Stal) is a major agricultural pest that plays a key role in reducing rice yield. Rapidclimate change has emphasized the necessity of cultivating multi-resistant cultivar rather than cultivar with single-resistance to specific pests. Accordingly, the Plant Molecular Breeding Laboratory of the Department of Applied Biosciences, Graduate School, Kyungpook NationalUniversity, Daegu 41566, Korea developed the ‘Drimi 1ho’ rice cultivar with enhanced resistance to blast and bacterial blight as well as BPHbreeding in 2008. In order to breed ‘Drimi 1ho’, ‘Samgang’ and ‘Nagdong’ were crossed and backcrossed with ‘Junam’, after which the pedigreebreeding method was applied. Finally, ‘Drimi 1ho’ was selected through analysis of resistance to insect and viral disease, yield, and grainquality. ‘Drimi1ho’ is strongly resistant to BPH and has a wide range of resistance to pests. ‘Drimi 1ho’ is also resistant to blast and bacterialblight (K1, K2, K3), which occurs most frequently and causes serious damage to crops in the Republic of Korea. The agricultural characteristicsof 'Drimi 1ho' were similar or improved from 'Junam'. In particular, ‘Junam’ is susceptible to BPH and bacterial blight. ‘Drimi 1ho’ madeup for the shortcomings of ‘Junam’ with acquired strong and broad-spectrum resistance. Finally, ‘Drimi 1ho’ had a yield of 563 kg/10 aunder normal cultivation and can thus be an adaptable cultivar in south mid-mountainous areas in the Republic of Korea (Registration No. 3685).

청청/낙동 배가반수체 집단에서 QTL을 통한 출수기와 수량관련 유전자좌 분석

장윤희,박재령,김경민 한국작물학회 2019 한국작물학회지 Vol.64 No.1

A quantitative trait loci (QTL) analysis of traits related to heading and yield was performed develop rice cultivars that are both early maturing and panicle weight type varieties. Our analysis included 120 strains of the Cheongcheong Nagdong doubled haploid (CNDH) variety. An observational growth experiment was conducted to identify genetic agronomic traits of CNDH. Heading date, ten plant weight, moisture, thousand grain weight, and yield had a normal distribution based on the frequency distribution table of the observational growth data. The QTL analysis found one heading-related and nine yield-related QTLs. The LOD of 2.85 was the largest in QTLs for heading date (QHD), 5.39 in QTLs for ten plant weight (QTPW), 3.92 in QTLs for moisture (QM), 4.80 in QTLs for thousand grain weight (QTGW), and 3.7 in QTLs for yield (QY). Genomic analysis detected 58 candidate genes on chromosome 2, 3, 7, 8, and 10. Among those, we found Rcd1 protein and OsERF3 gene in QM, MtN3 and zinc finger protein genes in QTGW, and OsNAC3 protein gene in QY. If further analysis reveals the presence of genes related to water content, thousand grain weight or yield in the CNDH stains, we can develop a selection of varieties that will be capable of coping with climate change and will contribute to global food problems. 본 연구는 2017년 CNDH 계통을 이용하여 출수기와 수량을 QTL 조사하여 다음과 같은 결과를 가졌다. 1. 도수분포표에서 QHD, QTPW, QM, QTGW, QY는 정규분포를 이루고 있었다. 2. 출수기 관련 QTLs은 총 1개가 잡혔고, 수량관련 QTLs 은 총 9개가 잡혔다. QHD에서는 LOD 2.85가 가장 컸고, QTPW에서는 5.39, QM에서는 3.92, QTGW에서는4.80, QY에서는 3.7이 가장 컸다. 3. 유전자 분석을 통해, 2, 3, 7, 8, 10번 염색체에서 총58개의 후보유전자를 찾았다. 이 중 수량요소인 QM에서 Rcd1 protein, OsERF3 유전자, QTGW에서 MtN3, Zinc finger protein 유전자, QY에서 OsNAC3 protein 유전자를 발견하였다. 4. 본 연구를 통해 발견된 CNDH 계통 내의 수분함량, 천립중, 수량에 관련된 유전자의 존재여부를 찾아낸다면조생종, 수중형에 가까운 품종을 개발하는 데 기초자료로 이용될 수 있을 것이라 판단된다.